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• Effect of G2548A polymorphism in the leptin gene on the BMI level in human population

  5-10

  Kritína Candráková

  Anna Trakovická

  Views:

  128

  The polymorphism in leptin (LEP 2548A) seems to influence obesity among others genes. The aim of this study is to investigate the effect of the G2548A polymorphism on body mass index. We included 79 people from Slovakia with some genetic relatedness and used barrels kit to isolate the genomic DNA from an adenoblast swab- from the salivary. PCR products were amplified by pursued polymorphisms and G2548A, we restriction-analyzed them and then we identified the specific fragments describing the presence of chosen SNP polymorphism by the agarose electrophoresis, to analyze SNP polymorphism by PCR-RFLP method.

  The LEP gene had increased frequency of G allele (0.5506). The most common genotype occurring in the gene LEP was heterozygous genotype (AG) and the least frequent genotype in LEP was AA (0.1899). Taking the age into account the BMI is higher if the G allele occurs in the LEP gene. Moreover, if the G allele genotype was situated in dominant form, then the highest average BMI was present.

  According to the results we can assume that the AA genotype (LEP) has a protective effect on the prevalence of obesity compared to the other genotypes.

• Agricultural land use and food safety

  55-60

  János Nagy

  Views:

  138

  The world’s food production needs to be doubled in order to cover the need of the population by 2050 even if it exceeds 9 billion. The output of agriculture is expected to increase by 1.7% every year until 2020 (OECD, FAO 2011) which is a major decrease in comparison with the average 2.6% increase of the previous decade. At the same time, the meat, dairy, sugar and vegetable oil consumption is likely to increase by 2020 to a higher extent than so far. Due to the increasing food prices, the amount of starving people will increase and food consumption will decrease – especially in developing countries – as people will be able to buy less and only cheaper food products. Also, obesity may become a more severe problem and the inequality of the population’s health status could increase.

  One of the most important elements of adapting to global climate change is food safety; therefore, it is especially important to breed new biological bases and to introduce production systems which contribute to adapting to changed circumstances.

• Effect of anthocyanin-rich Hungarian tart cherry extract on blood antioxidant status in C57BL/6J mice

  335-341

  Andrea Nemes

  Judit R. Homoki

  Rita Kiss

  László Stündl

  Judit Remenyik

  Views:

  189

  In this study male C57BL/6J (B6) mice were used to determine the possible effects of our Hungarian tart cherry extract in a chronic obesity mouse model on antioxidant capacity.

  The control group received standard mouse chow, the high fat control group was switched to high fat diet and tap water supplemented with 5% sucrose. The high fat+anthocyanin group received the high fat and sucrose diet, but dissolved in the drinking water they received anthocyanin-rich tart cherry extract. After six weeks, the antioxidant capacity and SOD activity were measured. Antioxidant capacity was decreased with a significant elevation of SOD activity. The tart cherry extract made a significant enhancement in antioxidant capacity and SOD activity. Our results show that chronic anthocyanin intake has a potential to enhance redox status associated with obesity.