Search
Search Results
-
Genetic structure of the Lipizzan horse breed in Hungary through the mare families
71-78Views:115Modern animal husbandry has drastically changed the genetic structure of some domestic species. The varieties, genotypes that we think we do not need at the moment can only be saved from extinction with the help of gene conservation. Traditional Hungarian horse breeds have a long history (more than 200 years) and a demonstrably different genetic structure from other horse breeds in Europe and the rest of the world. Consequently, their enormous genetic value is undoubted. The subject of our research was to study the structure of the mare families found in the population of the Lipizzan horse breed and the genetic structure of the Hungarian population. Out of the total 61 mare families in the world, 35 are present in Hungary. There are 11 Hungarian, 12 families of Fogaras, 7 original, 4 Croatian and 1 Slovenian mare families in the Hungarian population. The proportion of mare families in Hungary and Fogaras is almost the same. The proportion of Croatian and Slovenian families is negligible. In terms of their number, they are not significant in Hungary. For this reason they have little effect on the Hungarian Lipizzan population. Except one of the original mare families are in the same situation as the Croatian and Slovenian families. The Presciana / Bradamanta mare family is the most populous of all families due to their long stay in Hungary. The proportion of families is unbalanced. Seven mare families accounting for 56.36% of the total population. Given the basic requirements of gene conservation work, this condition is far from optimal.
-
Coincidences between molecular genetic and studbook data of gidrán mare families on the basis of mtDnA
69-73Views:182The traditional Hungarian horse breed, Gidran has been close to the edge of extinction several times. Despite the multiple bottleneck effect, the breed has retained a part of its genetic variability, and performed prominently in carriage driving and show-jumping competitions. Maintaining of the Gidran breed is important in the point of view of world heritage; because besides Hungary, smaller Gidran populations exist only in Bulgaria and Romania. Taking advantage of the special inheritance features of mtDNA, our study focused on two mtDNA regions of Gidran mares. Altogether, 251 hair samples from various Hungarian studs were examined. The analysis was successfully made in case of 251 samples of the cytochrome b and in case of 246 samples of D-loop regions. Because of the distinct mutation rates of the two mtDNA markers, the number of the haplotypes and the way of grouping samples into haplotypes was different. Our key finding was that most haplotypes may be compatible with mare families of the stud book; however incidental mistakes in stud book have occurred only in a few cases. Our results indicate the importance of the preservation and breeding those mare families, which are molecular genetically more diverse than the others, and are in the edge of extinction.
-
Identification of Hucul mare families by mtDNA markers
75-79Views:177Hundred animal species have disappeared during the last century. By this time, approximately one-third of domestic animals have been in the endangered category. Hucul horses are also in this category; furthermore saving the genetic diversity beside the race preservation is an important challenge as well. The number of mares and stallions is only one of the expressive elements of genetic diversity; together with their quality determine the genetic variability of this breed. Beyond that, if an exact breed can originates from more founders, it can be more renewed genetically. Stud book documents these data by registering the mare families and stallions’ genealogical lineage. Molecular genetics, especially mitochondrial DNA analysis can make the precise identification of mare families possible. As a result of these molecular based methods, protection of genetic diversity, as well as breed preservation became more reliable. After the primer designing, the optimal primer pair was chosen which targets a 1092 bp length DNA sequence in the cytochrome b region. After the successful PCR optimalisation, we determined 170 Hucul mares’ sequences. According to our results, the samples compose ten haplotypes, which are much less, than the registered number of mare families in the stud book. Further investigations are needed to reach more representative results, and drawn the further consequences.