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Effect of selenium supplement on proteome of chicken liver
9-13Views:137The aims of the present study were to optimize a two dimensional polyacrylamide gel electrophoresis method to chicken liver proteome and to determine the changes of protein expression caused by selenium. Twelve broiler chicken were used in this experiment. The selenium intake was 0,2 mg/kg in the control group (6 chicken) and 4,25 mg/kg in the experimental group (6 chicken). Using the optimized proteomic approach, we have succesfully separated 747 proteins in the experimental group and 741 proteins in the control group. We found six proteins, wich expressed only in the samples of experimental group. Further investigations need to determine these six proteins with mass spectrometry (MS) and look for the correlation between the physiological effects of selenium and the expression of these proteins.
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Single nucleotide polymorphism analysis in meat-production related genes in broiler chickens
79-82Views:157In broiler chickens, the intensive selection for growth rate, feed efficiency, body composition (breast muscle weight) traits in the last decades was successful. To improve economically important characteristics, it is possible to use molecular markers associated with meat production traits. The aim of this study was to examine genotype polymorphisms in ROSS 308 broilers for thyroid hormone responsive Spot14α, insulinlike growth factor 1 (IGF1), IGF-binding protein 2 (IGFBP2), somatostatin (SST) and prolactin (PRL) genes. A further goal of this investigation was to study the relationship between the polymorphisms and phenotypic characteristics.
In the investigated broiler population, the frequency for CC homozygous genotype was 0.77 in Spot14α (AY568628), AA homozygous genotype was 0.80 in IGF1 (M74176), GG homozygous genotype was 0.85 in IGFBP2 (U15086), DD homozygous genotype was 0.60 in PRL (FJ663023 or FJ434669). Only the AA homozygous genotype was found in SST (X60191). Chickens with AC genotype in Spot14α, and with GG genotype in IGFBP2 had higher body weight (BW) and carcass weight (CW), compared to CC and GT genotypes. However, the differences were not significant (P>0.05). There was significant association (P<0.05) between PRL genotypes and body and carcass weight, where chicken with homozygous DD surpassed individuals with homozygous II genotypes.
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Effect of change of natural efficiency indicators for profitability of broiler chicken fattening
57-61Views:115I consider it important that the fattening of broiler producers in an economic sense to remain alive in the present difficult situation. This does not mean to me, that just does not generate a loss, but they do remain in their activities after income. Because if you do not have sufficient income in a given sector then the technology becomes obsolete over time and income deficit due to the possible improvements are still lagging behind, resulting in competitive disadvantages occur. The natural efficiency remains a priority, because fundamentally determine our competitiveness in the broiler production. In the present study, scenario analysis can prove the veracity of my claim, in point of the three most important natural efficiency indicators. The natural efficiency indicators of a company compared to the natural effectiveness of our country and the our competitions (Western-European countries).
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A growth model to predict body weight and body composition of broilers
17-24Views:333Models predicting the nutrient partitioning and animal performance have been developed for decades. Nowadays, growth models are used in practical animal nutrition, and they have particular importance in precision livestock farming. The aim of the present study was to introduce a broiler model and to provide examples on model application. The model predicts protein and fat deposition as well as the body weight of an individual broiler chicken from digestible nutrient intake over time. Feed intake (FI) and the digestible nutrient content of the feed are inputs as well as some animal factors like: initial BW, feed intake at 1 and 2 kg of BW, precocity and mean protein deposition. The protein and energy metabolism is represented as in the classical nutrient partitioning models. The protein deposition (PD) is driven by digestible amino acid supply and is under “genetic control”, the so-called potential PD limits the actual PD if protein is oversupplied.
The authors discuss how the model can be used to simulate the animal response upon different scenarios. Examples are given to show that the diet might be limiting if some animal trait is changed. Applicability of the model has shown through running the model by using different feed strategies (three- vs five-phase-feeding) and variations with animal factors. In conclusion, growth models are useful tools to support decision making for defining the most suitable feeds used in a broiler farm. The model presented in this paper shows a high sensibility and flexibility to test different scenarios. By challenging the model with different inputs, the animal response in terms of changes in body weight and feed conversion can be understood more by studying the shift in deposition of chemical constituents. The examples provided in the present paper shows the benefit of using mathematical models and their applicability in precision nutrition. It can be concluded that the growth model helps to apply “from desired feed to desired food” concept.
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Some Variable Traits of Broiler Genotypes
25-30Views:132One the most effective ways to satisfy the requirements of mankind for food of animal origin is the fattening of chicken. Healthy and cheap meat with low fat and high protein content can be produced rapidly and in large quantities by broilers with low feed consumption. This paper presents the production results and carcass traits of three genotypes (Ross 308, Cobb 500, Ross broiler parent) females and males at the age of 42 days.
In both sexes, we found that the average body weight of the Ross parents genotype was higher (females: 2643 g; males: 3089 g) than the
average body weight of the other two hybrids – significant difference was found only between pullets. However, the smallest difference in the
average body weight between the two sexes (446 g) was found in this genotype. In both sexes, the lowest average weight was detectable in
the case of the Cobb 500 hybrids (females: 2443 g; males: 3052 g).The feed consumption ability of the females were worse – in all but one
case (Ross pullet) – than that of the males. For one kg of weight gain - in the order of genotypes – 1.79kg, 2.08kg and 2.08 kg of feed was
used. The same values for the males were as follows: 1.81kg, 1.80kg and 1.89 kg, respectively.
Carcass traits were examined and it was found that the average grillfertig weight, the weight of whole thigh meat and thigh meat of the
Ross parents’ genotype was higher than the average weight of the other two hybrids. Significant difference was found only in grillfertig- and
whole thigh meat weight. Pullets of the Ross parents’ paternal line had worse results in the average weight of breast meat with bone and
breast meat fillet than the other two genotypes. In the case of cocks was detectable the inverse of this: the average weight of breast meat with
bone was the highest in the cocks of the Ross parents’ maternal line. -
The effect of diet composition, a probiotic and a symbiotic treatment on the ileal microbiota composition of one-week-old broiler chickens
213-220Views:125A healthy microbiota present in the small intestine contributes significantly to small intestinal function, including digestion, nutrient absorption and health. The current study investigated the effects of a prebiotic, a probiotic and a symbiotic supplementation on ileal microbiota composition of broilers at 7 days of age. In a total of 574 male Ross 308 day-old chickens were divided into four groups using six replicate pens and 24 chickens per pen. A maise-soybean based control diet (C), a control diet supplemented with probiotics (Broilact; Br), a control diet supplemented with symbiotic (inulin, yeast, Bacillus subtilis; Sy) and a wheat based diet supplemented with wheat bran (W) were formulated. On day 7 of life, two chickens per pen were slaughtered and ileal chymus samples were collected. For microbiota analysis 16S rRNA (V3-V4 region) gene targeted Illumina MiSeq sequencing was used. Feeding all diets supplemented increased the diversity to varying degrees compared to the control (C) diet (p=0.006). As a conclusion, all supplementation substantially influenced ileal microbiota of broiler chickens at an early age. All these results could offer some information for the future study on the relationship between early intestinal microbiota and the compounds of the feed.
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The effect of β-glucan, carotenoids, oligosaccharides and anthocyanins on bacteria groups of excreta in broiler chickens
15-20Views:175This study was conducted to examine the effect of natural compounds, such as β-glucan, carotenoids, oligosaccharides, and anthocyanins in the diet on bacteria gropus of excreta in Ross 308 broiler chickens. Chickens were fed 5 diets: control (basal) diet, a diet supplemented by β-glucan at 0.05%, and diets supplemented by carotenoids, oligosaccharides, or anthocyanins at 0.5% of each compound. On experimental day 19, excreta were collected to determine the proportion of Lactobacillus, Bifidobacterium, Campylobacter, Clostridium, and Escherichia coli. Samples were collected aseptically and snap-frozen in liquid nitrogen. Bacterial DNA was isolated from samples, then polymerase chain reaction using primer pairs designed to the 16S rDNA of bacterial groups were applied to define the proportion of the mentioned bacteria. Another universal primer pair was used to amplify a region of 16S rDNA of all the examined bacteria. Proportion of each bacterial groups was determined relatively to the intensity of universal PCR product band by gel documenting system and ImageLab software. Based on the results, carotenoids and anthocyanins increased the proportion of Bifidobacterium, which might imply the beneficial effects of the mentioned compounds on the bacteria composition of excreta.
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Some strategic aspects of animal protein production
11-19Views:178The access to food shaped human societies and dietary models throughout the history of mankind. Animal protein consumption became a part of human culture. Data are presented showing the relationship of daily calorie and animal protein consumption as affected by capita GDP changes. Examples are presented how genetic improvement of animal and fodder plants influenced the resource efficiency and the overall environmental footprint per unit product. The two examples presented are: the dairy industry of the USA the 1944 and 2007 situation, and the Hungarian broiler chicken sector considering data relevant to 1930, 1960 and 2010. In both cases, dramatic improvements in resource efficiency could be demonstrated. The agricultural area required to animal feed production was reduced by more than 80% in both cases per unit product. Future possibilities are briefly discussed, referring to the still unutilized land reserves of the Globe, the new evolving technologies in progress inclusive the CASPR/Cas 9 genetic editing methods.
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Testing laboratory parameters of compost tea
31-36Views:383During the industrial production of broiler chicken, a large amount of manure is produced, of which easily contained nitrogen content (without pre-treatment) is released into the atmosphere as an air pollutant. In our experiments, we aimed to prepare compost tea, also known as water extract of compost, from pre-treated poultry manure in order to create a product can be utilized as liquid nutrient supply. The poultry manure source was the Baromfi-Coop Ltd. located in Nyírjákó, Hungary, where it was treated by composting. As a result of this pre-treatment of the poultry manure, its nutrient parameters improve and nitrogen is present in a form that is better utilized for plants. Furthermore, this product is suitable for further utilization and also can be the base material for a brand-new product. For this reason the effects of compost/water ratio, incubation time, low oxygen level, and extraction time on the parameters of the resulted product were studied in the frame of developing new soil-life enhancing microbial product, so-called compost tea.
Chemical parameters of the compost used as base material strongly determined the properties of the resulted compost tea, especially the ratio of the various nitrogen forms, their concentration and the salt content. It was found that adding water at a higher rate that means 1/40 and 1/50 mixing ratios results in more cost-effective production. In the experiment the compost tea were held under oxygen-poor conditions, therefore pH of the extractions decreased, which influenced the quality and quantity of their nutrient content.