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Assessing animal species at risk for SARS-CoV-2 transmission: Bioinformatic analysis based on Angiotensin-Converting-Enzyme (ACE2) homology in edible and other animals
127-137Views:432A novel coronavirus called SARS-CoV-2 was detected in December 2019, leading to the COVID-19 pandemic that began in Wuhan, China. This virus is classified as severe acute respiratory syndrome SARS-CoV-2 due to its significant similarities with the SARS-CoV virus. Initially, bats were recognized as the primary animal hosts, but later research indicated that other animals could also serve as reservoirs, posing health risks, particularly for those species consumed by humans. SARS-CoV-2 binds to the angiotensin-converting enzyme 2 (ACE2) as its cellular receptor, utilizing the receptor-binding domain (RBD) of its spike protein, much like SARS-CoV does.
The study aimed to identify animals, particularly edible animals, that may be susceptible to infection by SARS-CoV-2. This was achieved through bioinformatics techniques, including alignment analysis of genomic sequences from selected animals, identity percentages comparison, and phylogenetic analysis based on the interaction between ACE2 and the receptor-binding domain (RBD) of SARS-CoV-2. This analysis identified rabbits, donkeys, alpacas, horses, wild boars, field rats, and monkeys as potentially susceptible edible animals. Additionally, primates were highlighted due to their close genetic resemblance to humans. Overall, 22 animals worldwide were identified as susceptible, marking them as possible reservoirs and hosts for the virus, emphasizing the need for vigilance around animals that humans may contact or consume.
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Isolation of promoters of tissue and ripening-specific strawberry genes by TAIL-PCR and bioinformatic characterization of the sequences
91-99Views:148Isolation of ripening- and tissue-specific promoters has become a very important subject of the genetic regulation and plant physiology research in recent years. It could be possible to reveal the regulation of gene expression, and it may be a very useful approach in the biotechnology. In our work, we have isolated promoter regions of genes exhibiting ripening- and tissuespecific expression in our previous experiments, and the data were
characterized by bioinformatic methods. In the sequence of the ripening-specific Spatula and ACC-oxidase promoters (ACCoxidase is one of the key-enzymes of ethylene biosynthesis, directly related to the process of ripening); we could identify auxin- and ethylene-related cis-regulatory elements. This suggests that there is an interaction between ripening and ethylene-synthesis, in case of non-climacteric strawberry, too. We investigated the promoter regions of three green receptacle-specific genes (putative nitrilaselike protein, Ring transcription factor and an aquaporin protein)
and we could identify several regulatory elements, which refer to hormonal regulation. Additionally, we could find several cisacting elements which associated with stress-responsiveness and endospermium-specific expression.