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The Effect of Soil Coverings on Soil Respiration in Sandy Soil
21-25Views:229The purpose of our experiments is to study effect of different soil coverings (porous black polyethylene called agroszövet and black polyethylene) on CO2 production in sandy soil. The CO2 production was measured in our laboratory according to Witkamp (1966 cit. Szegi, 1979), after 5 days’ incubation period. Samples were taken off four times (March, May, July, September) in every year of the experiment. In May, July and September of 2000, the CO2 production was significantly higher in the control than in the treatment soil. With the exception of September, the value of CO2 production was significantly higher under black polyethylene than under agroszövet. In March and May of 2001, the soil under black polyethylene, and in July and September the control soil produced the greatest quantity of CO2. With the exception of July, significantly more CO2 was produced under black polyethylene than under agroszövet. To study the dynamic of CO2 production there was find a significantly higher value May and September of 2001 than 2000. Similarly significant higher CO2 production was detected in September than in the other months In average of two experimental years the difference between the produced CO2 under different coverings was occasionally. Explicit upward tendency in soil CO2 production was detected only in case of control soil. There was a medium (r=0,413) relationship observed between the moisture content and the CO2 producing ability of soil. To sum up the soil coverings had favourable effect on soil CO2 production very rearly, but they could help to conserve the moisture content of soil.
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Effect of Soil Covering on CO2 Production and Cellulose Decomposition Activity
198-201Views:113In our experiments the effect of different soil cover matters (agroszövet and black polyethylene) on the CO2 production capacity and on the soil cellulose decomposition activity was studied. We also examined the relationship between the CO2 production and the cellulose decomposition activity. The trial was carried out in an apple plantation in Újfehértó. The CO2 production were determined after Witkamp with 5 days’ incubation period, the cellulose decomposition activity was measured according to Unger. Results showed that CO2 production and cellulose decomposition activity was higher in control soil almost in every cases. In respect of CO2 production the black polyethylene gave better values than the agroszövet.
In 2000 the cellulose decomposition activity was significantly higher under covered soil than under uncovered every time. In spring of 2001 the controll but in summer and autumn agroszövet was significantly better. In summer and autumn black polyethylene prove non-significantly better than control. In average of two experimental years significantly higher cellulose decomposition activity was detected in covered soils than in control.
In the most occassion a close connection was observed between cellulose decomposition activity and CO2 production. In spite of this the correlation was not significant in controll at all. Significant relationship was only found in spring and summer of 2001 under agroszövet, in spring and autumn of 2000 and in summer of 2001 under black polyethylene. -
The impact of different fertilization methods on some microbiological soil characteristics
119-126Views:303In our experiment, we studied the impact of an organic fertilizer, Bactofil® A10 (half- and full dosage applied in field practice) and an artificial fertilizer of Ca(NO3)2 content in different dosages (20-40 mg kg-1) – in addition to control treatments – on two different soils (calcareous chernozem, humus sandy soil) in 2005-2006, the experiment was complemented with treatments applying 250% dosage (100 mg kg-1 N, Bactofil® A10 2.5 times the field dosage) and a compost from urban sewage (25 g kg-1 compost) was also tested on these two soil types. In the
experiment, several soil microbial parameters were studied. The experiment was set up at the Department of Agrochemistry and Soil Science using 1-kg pots.
Our laboratory experiments were performed at the soil microbiology laboratory of UD CAS Department of Agrochemistry and Soil Science, the total number of bacteria, microscopic fungi, nitrifying and aerob cellulose-decomposing bacteria were determined together with the CO2-production of soil, N content of the biomass and urease enzyme activity.
Statistical analysis of the data was done using the program SPSS 13.0, means of the measurements, deviation and significance values were calculated.
In 2005-2006, the effect of the different dosages of Bactofil® A10, and the Ca(NO3)2 fertilizer on the examined microbial parameters of calcareous chernozem and humus sandy soils can be summarized as follows:
• Concerning the total number of bacteria, both treatments were effective on calcareous chernozem soil, the higher (significant) increment in bacteria number was observed in the artificial fertilizer treatments, while in the humus sandy soil Bactofil treatments had a beneficial effect. The number of microscopic fungi also increased in both treatments, higher numbers were observed in the average of two years in the Bactofil treatments.
• The number of nitrifying bacteria was 2.5 times higher in both high-dosage treatments on calcareous chernozem soil, while on humus sandy soil a slight (not significant) increment was observed only int he high-dosage Bactofil treatment. The amount of aerob cellulose-decomposing bacteria significantly increased on calcareous chernozem soil in both the highdosage artificial fertilizer and the small-dosage Bactofil treatment, however, on humus sandy soil no significant increase was observed in either treatment.
• The CO2-production increased in both soil types, although it was not significant in either treatment. A higher (though not significant) soil respiration was observed in the Bactofil treatments in both soil types.
• The microbial biomass N values were significantly higher in the high-dosage Bactofil treatments, however, the high-dosage artificial fertilizer treatment also increased these values significantly on calcareous chernozem soil.
• On calcareous chernozem soil, urease activity was significantly increased and reduced by high-dosage artificial fertilizer treatments and Bactofil treatments, respectively. On humus sandy soil, urease activity was also reduced except for the high-dosage artificial fertilizer treatment. In 2007, the pot experiment with 250% dosages was complemented with the application of compost rich in organic matter, the results of these treatments are sumnmarized as follows:
• In the case of the total number of bacteria, all three treatments resulted in a significant increase on calcareous chernozem soil with the highest values in the Bactofil treatment. The Bactofil treatment was the most effective on the humus sandy soil, but the artificial fertilizer treatment also
resulted in a significant increment. In the case of the total number of fungi, Bactofil treatments resulted in the highest values on both soils, but the compost treatment also increased the number of fungi in calcareous chernozem significantly.
• The number of nitrifying bacteria was increased most (significantly) by the Bactofil and compost treatments on both soil types. The amount of cellulose-decomposing bacteria was significantly increased by he compost treatment on calcareous chernozem soil, while its effect was not significant on humus sandy soil. The number of these bacteria was increased significantly by the Bactofil treatment on humus sandy soil.
• On calcareous chernozem soil, all three treatments significantly increased CO2-production, while the compost treatments had the resulted in the largest increment in soil respiration on both soil types.
• The soil biomass N content was significantly increased in both soils by the compost treatment, while in the case of the humus sandy soil, the Bactofil treatment also resulted in a significant increment.
• Urease enzyme activity was significantly increased by the artificial fertilizer treatment on both soils. In calcareous chernozem soil, the Bactofil treatment resulted in a slight (not significant) reduction in enzyme activity. In humus sandy soil, the Bactoful treatment also resulted in a slight reduction, while the compost treatment increased (though not significantly) the urease activity.
Based on our results, it can be stated that all three treatments were effective with respect to the studied soil microbial parameters. For both the calcareous chernozem and the humus sandy soil, the organic fertilizer Bactofil and the compost with high organic matter content had a stronger effect on some soil microbial parameters than the artificial fertilizer. -
The effect of bentonite on the quantity change of soil microorganisms, the CO2-production and the activity of saccharase enzyme
287-293Views:229We examined the impact of bentonite – the perspective improving material of sandy soils – and treatments of livestock manure composted with bentonite on sandy soils, within the framework of a small-plot experiment.
The adjustment of the experiment was made on the Experiment Site of the Nyíregyháza Research Centre of the University of Debrecen, Centre of Agricultural Sciences (UD CAS). We collected soil samples from parcels treated with increasing bentonite doses (5, 10, 15, 20 t/ha) on the one hand, and from the parcels treated with livestock manure composted with increasing doses of bentonite, on the other.
We performed laboratory research in the soil microbiology laboratory of the Soil Science Faculty of UD CAS DAS, during which we determined the total number of bacteria, the quantity of microscopic fungi, the number of cellulose-decomposing bacteria, the CO2-production of the soil and the activity of saccharase enzyme.
During the evaluation of the examinations, we made a statistical analysis using SPSS 9.0. We determined the average of measurements, the standard deviation of controls, the standard deviation, the significance value and we also performed a correlation analysis.
Concerning the impacts of bentonite treatment and the treatment of livestock manure composted with bentonite on the examined microbiological features of sandy soil, we can summarize the following:
• Our results prove that the microbiologic activity of the soil has increased owing to the impact of bentonite treatments regarding total number of bacteria and the quantity of microscopic fungi. ”Pure bentonite” treatments – although not significantly in every case – increased these values, but larger doses decreased them. The treatments of livestock manure composted with bentonite resulted in a larger increase regarding both parameters.
• The number of cellulose-decomposing bacteria was increased by the low doses of both series, and was decreased by the higher doses. Higher bentonite doses decreased it in a higher – significant – degree than those of treatments of livestock manure composted with bentonite, whose low dose caused salient number of bacteria.
• Regarding the carbon-dioxide formation, we have experienced an increase even in the case of low dose treatments (nevertheless, the increase did not prove to be significant), but – similarly to the quantitative changes in the number of cellulose-decomposing bacteria – the large doses of both series of treatment decreased the CO2-production of the soil.
• Bentonite also increased the activity of saccharase enzyme significantly. We learned that”pure bentonite” treatments increased the activity of the enzyme to a higher degree than composted treatments. Moreover, it can be stated that the treatments of larger doses of both bentonite and livestock manure composted with bentonite have decreased the enzyme activity – not significantly, though.
• Based on the correlation analysis, it can be stated that as an impact of the treatments, the microbiological activity of the soil has also increased with the increase of the number of soil microbes, as in both treatment series we have experienced a tight positive correlation (r=0.81-0.82) between the change of total number of bacteria and the CO2-production of soil. In both treatments, there was a medium correlation between the total number of fungi and soil respiration (r=0.63-0.63). Furthermore, it can be stated that the usage of organic manure had a positive effect on the activity of cellulose-decomposing bacteria, as in this treatment series there was a positive correlation (r=0.65) between this physiological group and CO2-production. Both treatments prosperously impacted the activity of saccharase enzyme, because there was a medium correlation (r=0.62-0.64) between the activity of the enzyme and soil respiration. -
The impact of fertilisation on a few microbiological parameters of the carbon cycle
45-50Views:471The 30 years old long-term experiment of Látókép is continued in our experiments. The long-term fertilization experiment was set in 1983, and our sample was taken in spring 2014. The examinations of soil respiration processes and factors that influence soil respiration are required in optimal management. In our study, we interested to know how the growing levels of fertilization influence the microbial processes under nonirrigated and irrigated conditions in maize mono, bi, and triculture. The experimental results and those statistics suggest that the bi and triculture influenced higher microbial activity which was reflected in number of fungus, soil respiration, and microbial biomass carbon (MBC).
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Evaluation some important microbiological parameters of the carbon cycle in chernozem soils profiles
33-39Views:393Some chemical and microbiological properties of the carbon cycle were investigated in three chernozem soil profiles. The soil profiles originated from a long term fertilization experiment (potato) of the University of Debrecen, Látókép, Kryvyi Rig Botanic Garden (grassland) and a large-scale farm (sunflower) of Ukraine. The results of the organic C-content, total number of bacteria, microscopical fungi, cellulose decomposing bacteria, CO2-production, microbial biomass carbon and saccharase and dehydrogenase activities were compared and evaluated with the help of correlation analyses. Close correlation was found between the organic carbon content and the number of microscopical fungi,, saccharase and dehydrogenase enzymes’ activities, as well as close correlation was found between the dehydrogenase activity and microbial biomass-C and saccharase activity.
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Comparative analysis of certain soil microbiological characteristics of the carbon cycle
137-141Views:296In our researches, we examine the soil microbial parameters related to the carbon cycle. In this study, we compare the changes of microbial biomass carbon (MBC) and the soil CO2 production in soil samples which were taken in spring and autumn. The 30 years old long-term experiment of Debrecen-Látókép is continued in our experiments. The long-term fertilization experiment was set in 1983, and our sample was taken in spring 2014. The examinations of soil respiration processes and factors that influence soil respiration are required in optimal management. In our study, we interested to know how the growing levels of fertilization influence the soil respiration and microbial biomass carbon under non-irrigated and irrigated conditions in maize mono, bi, and triculture.