Mineral water consumption increased dramatically in the last 30 years. One reason for this change lies in the changing consumer behaviour: consumers are increasingly recognizing the importance of healthy nutrition and appreciate the beneficial nutritional physiological properties of mineral water. Local mineral water harmonizes well with import...ed waters. Bottled mineral water may travel several hundred kilometres until it reaches consumers. In the present study, the dynamic mechanical vibration caused by transporting on public roads was simulated in laboratory vibration tests then samples were subjected to microbiological examinations in compliance with legislations currently in force. Due to this vibration, the initial microbe count increased by two orders of magnitude, while after terminating the 4-hour mechanical action it decreased gradually. Growing dynamics of microbes constituting the total germ count at 22 °C and 37 °C were almost similar.
The aim of the current experiment was to optimize the creation process of single cell protein on plant-based substrate solution with the intention to improve end-product turn out by means of adding vitamin solution. Based on the results of the fermentation processes of yeast strains, it was concluded that the vitamin-supplementation produced it...s greatest effect on the dry matter production, primarily on the K. marxianus DSM 4908 strain, while it was less beneficent when it comes to the figures of wet cell mass. In addition, it can be assumed that vitamin supplementation increased the maximum specific rate of growth (μmax) and decreased the generation time (tg) significantly. In the case of the K. marxianus yeast strain on corn steep liquor treated with vitamin-supplementation, the highest (μmax) and the lowest (tg) data were observed [(0.226 h-1) and (4.4 h), respectively]. Based on the results it was found that K. marxianus DSM 4908 is expedient to be applied on corn steep liquor medium in order to determine its suitability to produce additive for feeding.