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Occurence and spreading of box tree moth (Cydalima perspectalis Walker 1859) on the North-East region of Great Hungarian Plain
45-50Views:205Box tree moth shows (Cydalima perspectalis) rapid spread in Europe. In Hungary it appeared first near to the western border of the country in 2011. In the eastern part of Hungary the first specimen was caught in 2015 with blacklight trap. Here we summarize its distribution in northern part of the Alföld (Great Hungarian Plain) on the basis of blacklight, pheromone and feeding attractant traps. We publish five new distribution data from northern part (4 from Hungary, 1 from Transcarpathia) and two from southern part of the Alföld. Beyond that the flight of three generations was observed both in the year 2015 and 2016.
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Elemental content of propolis samples from Pest, Zala and Bács-Kiskun county (Hungary)
149-153Views:261The elemental content of 61 raw propolis samples from Pest, Zala and Bács-Kiskun county (Hungary) were analyzed by ICP-OES. The content of Al, B, Ca, Cu, Fe, K, Mg, Mn, Na, P, S, Sr and Zn were measured in the samples. Median is higher than mean in all cases based on the summarized results. It can be explained by the outliers, moreover it has a non-normal distribution. The concentrations are in an extremely wide range, the ratio of the maximum and minimum is under 10 in case of six elements, between 10 and 35.1 in the case of other six elements,and more than 300 in case of Zn. Significant differences are between counties only in the case of B, K and P based on ANOVA. Other elements have no significant differences. The following order can be set up based on the mean concentrations and the wide ranges: K≥Ca>P≥S≥Fe≥Mg≥Al>Zn≥Na>Mn≥B>Cu≥Sr. Moreover it can be established, that the measured concentrations are near the same or in some cases lower than in other publications.
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The applicability of 10 ml cryotubes for sperm cryopreservation in a Hungarian carp landrace (Cyprinus carpio carpio morpha accuminatus)
93-97Views:245In our study, the comparison of 5 ml straw and 10 ml cryotube during sperm cryopreservation in a Hungarian carp landrace (Cyprinus carpio carpio morpha accuminatus) was carried out. Three different dilution ratios (1:1, 1:4 and 1:9) were also tested using the cryotube. A significantly higher pMOT was recorded using the cryotube in comparison with the straw. VCL and STR were similar in both groups. Cryopreservation had a negative effect on pMOT and VCl using the cryotube and also the straw where, STR was not reduced significantly. An increasing tendency was observed using higher dilution of sperm during cryopreservation; however, significant difference was not recorded between the three groups. VCL and STR were similar in all groups. Cryotube was applicable for the sperm cryopreservation of the Hungarian carp landrace. However, the standardization for the freezing method specified for this volume is recommended. The different dilution ratios needed be tested also during fertilization.
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The effect of the preparation method on the physical and chemical characteristics of propolis tinctures
163-168Views:278The effect of the preparation method was examined with regards to the physical and chemical characteristics of the propolis tincture, namely the extraction time and the ethanol content of the extraction solvent to the dry matter, polyphenol, flavonoid, phosphorus, calcium and copper concentration, respectively. The dry matter, the polyphenol and the flavonoid content were the lowest in the water extract of the propolis; however, significant increase was noticed depending on the extraction time. Significantly higher concentrations were found in 50 V/V% tinctures. The highest dry matter and flavonoid contents were analysed in 100 V/V% tinctures, whereas highest polyphenol content was found in 80 V/V% tinctures. However, the differences were not significant in several cases between latter tinctures. Moreover, the increase was not determined in some cases depending on the extraction time. Phosphorus and calcium concentrations were decreased depending on the increasing ethanol content, whereas copper concentration was increased up to 80 V/V%. Higher increase was found in the case of 0 and 50 V/V% extracts than in 80 and 100 V/V% tinctures. Moreover, in latter cases, no significant differences were found on several occasions, depending on the extraction time. There was no connection between the flavonoids and the calcium as well as the phosphorus content, whereas flavonoids may be made complex with copper. However, the amount of the possible complex was negligible.
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The comparison of sperm motility and density in four different goldfish (Carassius auratus) types
135-140Views:192Different goldfish types play an important role both in ornamental fish farming and science. Considering its historical background, the goldfish is a suitable model animal for the study of artificial selection as well as for developmental biological studies. Sperm motility and cell density is an important parameter in determining sperm quality. The aim of our study was to examine the effects of different goldfish types on the sperm quality. Several sperm motility parameters (progressive motility (pMOT, %), straight line velocity (VSL, μm s-1), curvilinear velocity (VCL, μm s-1), linearity (LIN, %), amplitude lateral Head Displacement (ALH, μm), Beat Cross Frequency (BCF, Hz)) of four different goldfish types (Common goldfish-“wild type” N=5, Shubunkin N=4, Black Moor N=4, Oranda N=5) was compared during 60 hours (at 12-hour intervals) at refrigerated storage (4 °C). The variability of sperm density was also investigated in all types. A similar cell concentration was determined in the four goldfish variants (Common goldfish 2.01*1010 ± 3.46*109; Shubunkin 1.71*1010 ± 3.25*109; Black Moor 1.66*1010 ± 3.02*109; Oranda 1.56*1010 ± 5.83*109). Statistically significant difference between the 4 goldfish types in the motility parameters and cell density was not noted. However, a decreasing tendency in Black Moor sperm motility parameters (pMOT, VCL and VSL) was observable, as well as a reduced spermatozoa density in Oranda was also recorded. Our results can contribute to the improvement of the common hatchery propagation of goldfish. Future studies can add more evidence of the possible effects of artificial selection on the reproduction in different goldfish types.
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Optimizing fish structure in angling ponds focusing on white fish
33-36Views:184According to new amendments of laws, commercial fishing licenses of natural waters shall not be issued from the calendar year 2016 in Hungary. However, the most settlement of white fish to angler ponds originated from natural waters. Currently, there is no sufficient quantity of fish species belonging to this group available to fulfil the settlement commitments of fish farmers. The aim of this recent study is to develop a breeding and production technology that is suitable for settlement of large quantities of this fish species of appropriate length of (4–5 cm). The following methods are investigated: aquaculture cage system (pond-in-pond system), monoculture and mixed population with different ratio of white fish in fish structure. Due to the different environmental needs of different fish species, many parameters need to be investigated, for example: stocking density, feeding, oxygen level, monoculture or mixed populations, growth potential and mortality. A preliminary experiment was carried out following the artificial propagation of ide (Leuciscus idus) following a 21-day larval rearing experiment in which the effect of different stoking density was investigated for mortality and growth. On the basis of the expected results, the optimal conditions for the aquaculture cage system will be described, regarding to the white fish stock.