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  • Effects of paraffine oil on leaf and berry mycobiota on two grape varieties
    61-66
    Views:
    576

    Application of fungicides have advantages and also some direct or indirect disadvantages, such as imbalance and/or fungicide resistance in microbe population. To avoid these problems the development of alternative, eco-friendly methods like mostly spraying with oils are in the focus nowadays. The investigations of the effects of fungicides on microbiota in some cultivations can give a more complex view to this topic and developmental possibilities. In the present study, our aim was testing of the effects of paraffine oil (as alternative fungicide) on microbial properties (CFU and rate of filamentous fungi and yeasts) of Chardonnay and Kékfrankos leaves and berries.

    Our results from 2014 showed that the application of paraffine oil as sole spray agent can decrease the presence of saprophytic filamentous fungi on the berries of Chardonnay (susceptible for fungal infections). In the case of Kékfrankos berries opposite properties were observed, which may be the result of the absorption of oil by the thick wax layer of this variety. The oil treatment did not affect the yeast population of Chardonnay and Kékfrankos berries contrary to negative effect of the regular pesticide treatment. The selective fungicide effect of paraffine oil against filamentous fungal population caused the accumulation of yeast cells in the mycobiota of grape berries. The careful use of this yeasts in spontaneous fermentation can improve the aroma profile of wines. The year of 2015 did no prefer the growth of fungi, therefore no interesting properties were detected in the mycobiota of grape varieties. The occurence of the harmful saprophytic filamentous fungi predicted to be increased in mild climate agricultures as the result of the climate change.

    In summary, the paraffine oils are seem to be promising tools for the eco-friendly control of harmful fungi of grapes.

  • Element content analyses in the Institute for Food Sciences, Quality Assurance and Microbiology
    203-207
    Views:
    435

    The role of chemical elements to ensure and promote our health is undisputed. Some of them are essential for plants, animals and human, others can cause diseases. The major source of mineral constituents is food, drinking water has a minor contribution to it, so the knowledge of elemental intake through food is crucial and needs continuous monitoring and by this way it promotes the food quality assurance and dietetics.
    With the evolution of spectroscopic methods increasingly lower concentrations could be determined, so the elemental composition of a sample could be more precisely and fully described. Due to the results the gathered knowledge up to the present is supported and new observations can be done helping us to understand such complex systems as biological organisms are.
    The quality of a food is determined by the full process of its production, consequently it starts with agricultural production so elemental-analysis usually cover the whole soil – plant – (animal) – food chain, by this way the „Fork-to-Farm” precept is true in elemental analysis field also.
    The history of elemental analysis in the University of Debrecen, Centre for Agricultural and Applied Economic Sciences, Faculty of Agricultural and Food Sciences and Environmental Management, Institute of Food Processing, Quality Assurance and Microbiology goes back to 1980s when the so called Regional Measurement Central gave the background for research. The continuous deployment resulted in an obtain of an inductively coupled plasma atomic emission spectrometer (ICP-AES) in 1988, which extended the scope of examinations due to its excellent performance characteristics
    compared to flame atom absorption (FAAS) and flame emission spectrometers (FES). The instrumental park retain up to date correlate to the developing analytical techniques due to acquiring a newer ICPAES in 1998 and an inductively coupled plasma mass spectrometer in 2004 – which sensitivity is three order of magnitude better compared to ICP-AES. The Institute supports the work with its own ICP-AES and ICP-MS since 2011. 

  • Development of the antioxidant indexes (FRAP, TFC, TPC) of scabbing resistant apple varieties in storage
    89-94
    Views:
    301

    One of the most important groups of bioactive substances in apples are antioxidants, which have a health-preserving effect. The amount of these compounds varies not only during the ripening of the apple fruit, but also continuously during post-harvest storage. A growing group of health-conscious consumers are looking for apple varieties with a nutritional value that satisfies their needs.

    In the scope of our research, we examined 5 apple varieties originating from the Derecske horticultural site of KITE cPlc. in 2016. The plantation was planted to be suitable for intensive apple production. Samples were collected at the time of maturity (August-October) of the given variety. The 5 examined apple varieties (Gaia, Isaaq, Modí, Smeralda and Fujion) are all resistant to apple scab (fungal disease caused by Venturia inaequalis). The 2-month storage experiment was conducted at 16–17 °C, which had an aggressive effect on our stored apples. Total polyphenol (TPC) and flavonoid (TFC) content as well as FRAP values of the apples were measured. Measurements were taken immediately after harvest and after 1 and 2 months of storage. Our results were evaluated by using the IBM SPSS Statistics 20 software. Our aim was to compare the antioxidant results of 5 scab-resistant apple varieties, which change continuously during storage.

    The antioxidant content of our apple varieties increased during 2 months storage. The best result was achieved by the Modí apple variety (FRAP: 467.32 mg/100g; TFC: 317.76 mg/100g; TPC: 1771.97 mg/100g). Consumers may want to consume apples stored for a longer period of time if they are to absorb large amounts of antioxidants.

  • Flesh firmness examination of scab-resistant apple varieties in a storage experiment
    93-98
    Views:
    340

    Of the different physical characteristics of fruits, one of the most essential qualities is flesh firmness, as is an important parameter when selling fresh apples, processing the fruit (processing industry) and during its storage. In the scope of our research, we examined five apple varieties originating from the Derecske horticultural site of KITE cPlc. in 2016. The plantation was planted to be suitable for intensive apple production. Samples were collected at the time of maturity (August-October) of the given variety. The five examined apple varieties (Gaia, Isaaq, Modí, Smeralda and Fujion) are all resistant to apple scab (fungal disease caused by Venturia inaequalis). The two-month storage experiment was conducted at 16-17 °C, which had an aggressive effect on our stored apples. Flesh firmness, weight and diameter were measured on three dates for each of the five apple varieties. Our results were evaluated using IBM SPSS Statistics 20 software. We aimed to compare flesh firmness, weight and diameter of the five apple varieties and their changes during storage. Isaaq (86.34 N/cm2), Modí (94.06 N/cm2) and Fujion (84.90 N/cm2) had outstanding initial flesh firmness results. The Modí apple variety provided the best storage parameters.

  • Impact of fertilisation and the fluctuation of precipitation on the ecophysical and production characteristics of maize
    39-44
    Views:
    571

    The aim of this study was to analyse the problems caused by the unfavourable (dry and wet) weather and its consequences in the R1 growth stage of maize (Zeamays L.), as well as their management and the alternatives of preventing yield reduction by using agrotechnical measures fertilisation, irrigation), also, we wanted to examine whether the Chl content measured in the R1 growth phase provides reliable prediction of yield per hectare.

    The examinations were carried out in a moderately warm and dry production area at the Látókép Experiment Site of the University of Debrecen, Centre for Agricultural Sciences on calcareous chernozem soil in 2007 and 2008. Six different N doses (0,30,60,90,120,150 kg ha-1) were used in the irrigated and non-irrigated treatments of the field experiment.

    The results showed that there is a significant strong positive correlation between Chl content and yield both in the non-irrigated (P<0.001, R=0.777) and the irrigated (P<0.001, R=0.801) treatment. The results of the correlation analyses performed yearly showed that weather factors significantly influence the strength of correlations, but these correlations are always positive.

    The Chl content of maize leaves provided a reliable prediction of yield per hectare in the R1 growth stage. In the irrigated treatment, the correlation is always closer than in the non-irrigated treatment.

  • The effect of and interaction between the biological bases and the agrotechnical factors on maize yield
    83-87
    Views:
    301
    The effect of and interaction between the biological bases and the agrotechnical factors on maize yield In our research, we examined the effect of the hybrid, the nutrient supply, the number of plants and the abiotic factors (temperature, amount of precipitation) on the yield, crop quality and yield stability of maize. We devoted special attention to the natural nutrient utilization ability and fertilizer reaction of maize. The experiment took place in Hajdúszoboszló on chernozem soil, on a nearly 8 ha field. The size of one plot was 206 m2; therefore, this experiment was half-industrial. We tested six hybrids with different genetic characteristics and growing seasons.
    We analysed the correlation between the nutrient supply and the yield of maize hybrids with a control treatment (treatment without fertilization) and with N 80, P2O5 60, K2O 70 kg ha-1 and N 160, P2O5 120, K2O 140 kg ha-1 fertilizer treatments. The yield increasing effect of the fertilizer also depended on the number of plants per hectare to a great extent. The number of plants of the six tested hybrids was 60, 70, and 80 thousand plants ha-1.
    In 2015, the highest yield was produced by hybrid P9241 with N80+PK and 70 thousand plants per hectare. With the N160+PK fertilizer dosage, the same hybrid responded the best, followed by hybrids P9486 and DKC4717. Using the same fertilizer treatment, the 80 thousand plants per hectare population density resulted in decrease in the yield with most of the examined hybrids. In 2016, with the increase in the number of plants per hectare, even with non-fertilised treatment (control treatment), the yield could be increased in the case of each hybrid.
    Averaged over the different hybrids and fertilizer treatments, applying 80 thousand plants ha-1 instead of 60 thousand resulted in 1.0 ha-1 yield increase. In 2017, the number of plants had a slighter effect. With N160+PK treatment, in most cases no significant difference can be observed. The value of LSD5%: plant number: 0.20 t ha-1, hybrid: 0.28 t ha-1, interaction: 0.48 t ha-1. With N160+PK treatment, the hybrids produced yields between 10.07 and 12.45 t ha-1. When examining the three years in the average of the number of plants, with treatment without fertilisation, the average yield of hybrids reached 7.53 t ha-1. With N80+PK treatment, this value was 9.71 t ha-1 and with doubling the fertilizer dosage, this value increased to 10.42 t ha-1. No economic profit was gained as a result of applying double dosage of fertilizer; therefore, the N80+PK dosage can be considered ideal.
  • Examination of different fungicides against Macrophomina phaseolina in laboratory conditions
    65-69
    Views:
    419

    In Hungary, sunflower is the third most important arable crop, which has a lot of pathogenic fungi. One of these fungi is the Macrophomina phaseolina, which is a well-known fungus in all over the world, since this pathogen has more than 700 host plants. In Hungary, several host plants can be found as well. The M. phaseolina produces microsclerotia, which can survive in the soil and residues for almost 10 years. For now, there is no efficient treatment against this pathogen because of this fungus, since it is extremely resistant and cannot be destroyed easily. The only effective treatment against the fungus is genetic defence. In this study, three different fungicides were tested in vitro against the fungus. The Mirage (prochloraz) seemed to be the most effective fungicide as it completely arrested the hyphal growth. In contrast, the Amistar Xtra (azoxystrobin and ciprochonazol) has only a minor effect on the growth of M. phaseolina. Thirdly, the Retengo (pyrachlostrobin) arrested the hyhpal growth of the fungus with 71% at 100 ppm, in other words, the use of this fungicide seems promising.