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Integration of Transformation Technology and Conventional Plant Breeding of Cereal Plants
75-80Views:183The integration of plant breeding and plant transformation is needed because we have to create a homozygous genotype of great agronomic value by conventional breeding before the application of genetic technology with which we modify it by using a gene or genome sequence. The aim of integrated plant breeding is to trigger such advantegous changes by genetic technology which can not be achieved via conventional breeding or just with considerably weaker efficacy. By transformation, the plant’s agronomic performance, the efficiency and security of its production will improve and it will enable more versatile uses of the plant. Genetic technology is one sequence of a new plant variety’ breeding. To create a transgenic variety, the isolation of a gene or a sequence of a gene from the donor genome for tranformation, a homozygous plant or target genome that is suitable for transformation and is created via conventional breeding methods, an effective transformation technique and the establishment of the new variety from the transformed, fertile plant are needed. The transgenic plant should be made suitable for establishing a variety by conventional breeding so that it could be produced securely, its growing could contribute to the development of modern, sustainable agriculture, its seed could be produced profitably, it could meet the reqiurements of DUS and that the changes indicated by the transgene could provide such economic advantages compared to the original variety, which have real commercial value.
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Changes of the fatty acid composition of sprouts during germination
89-92Views:327During our research we studied the fat content and fatty acid composition during the germination and sprouting periods of the most important sprouts: wheat, lentil, alfalfa, radish and sunflower seed. In this article we present our research results during this sprouting study. The concentration of the saturated fatty acids (palmitic acid, stearic acid) decreased, the concentration of the unsaturated fatty acids increased during germination, but the tendency was not so high than was published in the literature.
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Mass occurrence of a Phoma-like fungus on common ragweed (Ambrosia artemisiifolia) in Hajdúság region, East Hungary
55-60Views:427Common ragweed (Ambrosia artemisiifolia L.) is one of the most important, allergenic weed species in Hungary. A. artemisiifolia invades both a broad range of often disturbed areas (brownfields) and either undisturbed ones like waste lands, roadsides, riverbanks and railway tracks. In field crops it can cause considerable yield losses mainly in sunflower, maize, cereals and soybean. In Hungary many inhabitants suffer from allergy caused by Ambrosia pollen which results a serious human-health risk. The aim of the control is to prevent flowering and seed propagation of A. artemisiifolia. Until now the occurrence of numerous pathogenic fungi which attack common ragweed plants have been identified in Hungary, however there is not yet available biological weed control program because of shortage in acceptable effectiveness, and endangering cultural plant species. During our weed surveys in the region of Hajdúság (East-Hungary) we found numerous common ragweed plants showing heavy necrotic lesions on leaves and stems. The objective of this study was to identify the fungus which was isolated from diseased tissues of common ragweed (A. artemisiifolia). The identification of fungus based on morphological characters of colonies and features of conidia and chlamydospores developed on malt extract agar (MEA) plates. After examination of axenic cultures we revealed that the fungus isolated from the leaves ands stems of common ragweed was a Phoma-like species.
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Effect of Storage on Fruitquality of Apricot (Prunus Armeniaca)
164-169Views:304The quality of a product is determined by numerous characteristics. As quality characteristics are polygenic, they cannot be improved easily. Moreover, there often is a negative correlation between the different parameters in the case of apricot. The firmness of fruits decreases with increasing size. The year effect also has a great influence on the expression of the potential quality of the given cultivar.
Our examinations were carried out in 2004 at Boldogkőváralja, in the cold storage plant of the Northcot Ltd. We monitored the development of fruits from fructification until harvest, and also the quality changes during storage. 100 fruits were observed from each cultivar directly after harvest. We measured height, width, weight, firmness, dry matter content and seed weight. From each cultivar, 100 fruits were stored, of which 25 fruits were observed each week. The value and the dynamics of the changes in the parameters compared to the values at harvest varied greatly for the different cultivars. Strong correlations between the parameters have not been found within one cultivar either. The heavier fruits did not lose more weight, either in absolute value or relatively, than the lighter fruits. The increase in the firmness of fruits was not accompanied with a desirable decrease in the dry matter content. -
Investigation of directions of crossing in maize (Zea mays L.)
43-48Views:260In Hungary, we examined eight features of twelve direct cross hybrids over a period of three years. The twelve hybrids were derived from direct and reciprocal crosses of four parental lines. We did not find significant differences in the cases of stalk diameter and leaf number in the average of 3 years in any of the hybrids. Statistically explainable differences in primary branches were observed in hybrids UDH5 and UDH8 and also in UDH6 and UDH11. It was evident in both instances that degrees of heterosis in reciprocal crosses far exceeded those of direct ones. High tassel branch number was dominant over the low one; consequently, parents with higher tassel numbers enforced their effects during the formation of this trait in hybrids. We experienced positive correlation (r=0.67**) between plant height and main ear height. A positive correlation (r=0.89**) was also found between the tassel length of the main axis above the lowest and the upper side branch. We observed a medium correlation (r=-0.42**) between the number of primary lateral branches and plant height. The number of primary lateral branches showed the highest heterosis. These results can be utilized in practical selection and seed production.
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Land use, water management
81-87Views:603Due to the prognosed population increase to 9.2 billion people by 2050, the world’s crop production does not have any other chance than to increase production. This demand is a huge challenge for agriculture. Based on the forecasts, the growth rate of production of the main cereals will decrease as a result of the effect of soil, water, the increasing fuel and fertiliser prices and the impacts of climate change. Methods ensuring sustainability have to be preferred. Precision agriculture is the most effective method of crop production. We have to apply minimum cultivation in order to protect the soil surface, maintain its moisture content and increase its water reception ability. In addition to the localised use of fertiliser, sowing seed, irrigation and pesticides, it is also important to apply them in a targeted way on the basis of plot imaging. The use of the new technology results in significant cost saving and it could also reduce environmental load.
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Evaluation of the role of common vetch (Vicia sativa L.) green manure in crop rotations
161-171Views:434Common vetch (Vicia sativa L.) is an annual legume, grown as green manure provide rapid soil cover, can increase soil moisture and organic matter content and reduce soil erosion during fall. During the fallow period, legumes grown as catch crops are known by releasing large amounts of mineral nitrogen (N) for the subsequent crop. By taking advantage of these benefits, it is possible to increase the yield of the next crop in an environmentally friendly and sustainable way. The goal of this study was to determine the value of common vetch as a green manure, considering its effect on soil conditions and the yield of next crops. We examined three different common vetch seed rate as a green manure in a crop rotation with triticale, oat and corn. Next to the green manured treatments, we used fertilized and bare fallow control treatment for comparison. In our study we evaluated the aboveground biomass weight of spring vetch green manure and its effect on the moisture content of the soil. We examined the green manure’s effect on the next crops plant height and yield. We found that the moisture content of the green manured plots was significantly higher during summer drought. On the green manured plots, 37.9% higher triticale yield, 50% higher oat yield and 44% higher corn yield were measured compared to the control plots. The insertion of spring vetch green manuring into crop rotations could be a good alternative to sustainable nutrient replenishment methods. It can be used to reduce the input needs of farming, reduce carbon footprint, contribute to the protection of soils and increase the organic matter content of the soil.
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Effect of dietary fatty acids on the oxidative stability and the colour of broiler meat
25-30Views:204The aim of our investigation was to determine the effects of increased PUFA (polyunsaturated fatty acids) content on the colour, total pigment content, organoleptic characteristics and oxidative stability of poultry meat. The experiment was carried out with 1200 Ross-308 cock chicklings. Animals were fed with a 3 phase diet, and in each phase, additional fat was added to the feed. The isocaloric and isonitrogenic feed was produced as the breeder organization suggested; only the fat content differed (4 treatments: pig fat (lard), sunflower oil, soy oil, flax-seed oil).
The different fat complements did not influence broiler production. However, the fatty acid composition of meat was similar to the fatty acid composition of feed (additional fats). The analyses of meat samples, after a storage period, did not significantly prove the possible negative effects of higher PUFA content. -
Growth regulators influence on stability of shoots and ascorbic acid content at cadmium and nickel joint action
9-12Views:227It is shown that for a maize the most effective protector of cadmium and nickel influence was zeastimulin, for a pea – agrostimulin.A protective function of emistim C for both cultures was insignificant. It is set that zeastimulin is influential in the increased metals absorption of a root system, however substantially (on 15% for a nickel and twice for a cadmium) reduces their translocation to above-ground part of plants. Previous treatment of pea seed by agristimulin also intensified the accumulation of nickel roots on 60% and did not influence on cadmium absorption and toxicants translocation to tissues of assimilatory organs. The protector role of growth regulators to cadmium and nickel joint influence shows up in the increase of ascorbic acid maintenance in the roots cells of both species to 34%, where as in leaves – at a maize resulted in the lowering of vitamin С amount (on 28%), but at a pea – increase on 20%.
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Performance of agricultural factors on yield of sweet corn (Zea mays L. Saccharata ) - A review
143-156Views:964Sweet corn producers and industries require more reliable cultivars which could be accomplished by hybrid breeding. However, progressive phenological growth may be affected by different factors. In this paper, we analyze the key factors that determine the growth and yield of sweet corn. Environmental factors such as temperature and photoperiod were strong determinants of dates of flowering and harvest which are often crucial to yield in diverse climates and agricultural systems, besides the country's pedological conditions, especially soil fertility, affected phenological development. The effectiveness of fertilization in improving sweet corn growth performance was significantly influenced by the soil characteristics, the water supply, the genotype, and the agrotechnological factors. Therefore, genetic improvement of hybrids should be incorporated into the climate and soil elements to stabilize sweet corn yields in various agroecosystems. Decisions made in the sowing period are very significant, as up to 30% of the obtained yield may depend on making the proper choice. Deviation from the optimum date (either early or late sowing) may decrease yield. When deciding about the sowing date of maize, one needs to consider climate, soil quality, geographical location, temperature, weed infestation, sowing seed quality, and the ripening time of the hybrid to be produced.
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Analysis of the situation of domestic game damage and forage ground management
5-8Views:366Game damage is an important problem in home wildlife management, because game managers pay more and more money for game damage year by year. Crop fields can do an appreciable part in reduction of game damages. The aim of the research is to survey the situation of domestic game damage and wildlife forage ground management. (Which are the most used plants on crop fields, how current are the using of seed mixtures, which are the most typical game damages, as well as which are the most applied game control methods?)
It can be stated that the number of plant species sown on crop fields is quite low. Mostly corn and alfalfa are raised, though besides those many other plants are suitable to grow on crop fields. Rising value of game damage and big proportion of the agricultural game damage are well traceable from the questionnaires, too.
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The effect of irrigation on the yield and quality of maize (Zea mays L.) hybrids
143-147Views:358In this study, the effect of water supply on the quality and productivity of different maize hybrids was observed.
Maize production is influenced by many agro-technical factors such as nutrient supply, plant density, environmental factors, water supply and temperature. Good soil quality and adequate technology significantly reduce the unfavourable effect of crop year. The impact of fertilization, crop rotation, irrigation and plant density on maize yield was greatly affected by crop year and crop rotation. The main constituent (69–75% dry matter concentration) of maize seed is starch, however, its increase can be achieved only to a limited extent, as increasing the starch content will reduce other parameters, especially protein content. Significant nutrient content improvements can be achieved by appropriate hybrid-site connection and the use of adequate technology. Protein content reduction with optimal water supply can be positively influenced by the appropriate nutrient supply for the hybrid.
The experiments were carried out at the Látókép Experimental Site of Crop Production on calcareous chernozem soil. The weather of the examined year, which was partly favorable for maize and partly unfavourable in other respects, was also reflected in the development of maize and yields achieved.
In 2019, we were examining the hybrids of Kamaria (FAO 370), P 9903 (FAO 390), DKC 4351 (FAO 370) and KWS Kamparis (FAO 350–400). Due to the dry soil condition, sowing was delayed, however, the hybrids emerged ideally because rain arrived soon after sowing, which facilitated initial development. Young plants evolved rapidly and dynamically in the case of all hybrids. In terms of heat-demanding bread, the month of May was unfavourable to temperatures below the annual average, but the higher amount rainfall helped the development. However, due to the drier period in early July, 25–25 mm of irrigation water was applied to half of the experimental areas on July 1 and 15.
The aim of our research was to determine the best population density for hybrids under favourable soil conditions (calcareous chernozem soil). In both irrigated and dry conditions, a crop density of 75,000 crops were the most favourable for the Kamaria hybrid. The P9903 hybrid in the case of a crop density of 85 crops proved to be ideal and the DKC4351 had an optimal population density of 95,000.
However, it should be taken into consideration that, in the case of soils with poor water management, the drought sensitivity of the crop stand may increase at a population density of 95,000.
As a result of irrigation, yield increased and the difference between the examined plant numbers decreased. The yield growth was relatively moderate (341 kg ha-1 – 1053 kg ha-1), which makes the economicalness of irrigation doubtful in the given year.
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Sequence stability at SSR, ISSR and mtDNA loci of common millet (Panicum miliaceum) from the middle ages
10-19Views:321Seed remains of medieval millet, recovered from a 15th century layer (King’s Palace, Budapest, Hungary), showed reddish yellow grain color after rehydrating on tissue culture medium that was close to grain color of modern cultivar Omszkoje. aDNA of medieval c. millet was extracted successfully, analyzed and compared to modern common millets by ISSR, SSR, CAPS and mtDNA. Analyses of fragments and sequences revealed
polymorphism at seven ISSR loci (22 alleles) and at the 5S-18S rDNA locus of mtDNA. CAPS analysis of the 5S-18S rDNA fragment revealed no SNPs in the restriction sites of six endonucleases TaqI, BsuRI, HinfI, MboI, AluI and RsaI. Sequence alignments of the restriction fragments RsaI also revealed
consensus sequence in the medieval sample compared to a modern variety. Morphological characterization of twenty common millet (Panicum miliaceum L., 2n=4×=36) cultivars and landraces revealed four distinct clusters which were apparently consistent with the grain colors of black, black and brown, red, yellow, and white. In the comparative AFLP, SSR and mtDNA analysis modern millet cv. ‘Topáz’ was used. AFLP analysis revealed that extensive DNA degradation had occurred in the 4th CENT. ancient millet resulting in only 2 (1.2%) AFLP fragments (98.8% degradation),
compared to the 15th CENT. medieval millet with 158 (40%) fragments (60% degradation) and modern millet cv. ‘Topáz’ with 264 fragments (100%). Eight AFLP fragments were sequenced after reamplification and cloning. Microsatellite (SSR) analysis at the nuclear gln4, sh1, rps28 and rps15 loci of the medieval DNA revealed one SNP (single nucleotide polymorphism) at the 29th position (A to G) of rps28 locus compared to modern millet.
Mitochondrial (mtDNA) fragment (MboI) amplified at the 5S-18S-rDNA locus in the medieval millet showed no molecular changes compared to modern millet. The results underline the significance of survived aDNA extraction and analysis of excavated seeds for comparative analysis and molecular reconstruction of ancient and extinct plant genotypes. An attempted phenotype reconstruction indicated that medieval common millet showed the closest morphological similarity to modern millet cultivar Omszkoje. -
Susceptibility of stem infected sweet corn hybrids to common smut disease
55-57Views:909The common smut of maize (corn smut, Ustilago maydis /DC/ Corda) can cause large economic losses in susceptible sweet corn hybrids as well. The protection against this pathogen is fundamentally based on prevention. Many methods to control corn smut have been recommended or evaluated, including crop rotation, sanitation, seed treatments, modification of fertility, and biological control. In spite of these frequently mentioned control strategies, the host resistance seems to be the only effective method to manage common smut in those areas where Ustilago maydis is prevalent.