Search
Search Results
-
Application of the SDS-PAGE method for the characterisation of winter wheat flour quality
112-118Views:101The principle, development and importance of the SDS-PAGE method are presented in this article. The SDS-PAGE method has become one of the basic methods of molecular biological research, because it is widely applicable and its sensitivity is excellent in the separation of wheat storage proteins.
We have shown the application of this method with a concrete example. It was also tested whether, it was possible to obtain a better baking quality product from a large amount of poor quality less valuable wheat by fractioning the flour according to particle sizes after grinding. We studied the rheological properties of flours with different particle size fractions from the original flour. The baking quality of the original flour was B2. The 125-90 and 90-63 μm fractions have significantly better baking quality (B1) than the original flour. The protein contents of these flour fractions were also significantly higher than the protein content of the original flour. We had a question: what has influenced the baking quality: the protein content or other factors? We searched for an explanation on these results in the protein composition of the flour samples. We studied the distribution of glutenin-fractions by SDS-PAGE method and evaluated them. We found with correlation analysis that the amount of LMW-Glutenin D-group (52-60 kDa) is in a strong, negative correlation to the baking quality (r = – 0.855*). Therefore, the baking quality of flour samples was influenced by this glutenin fraction. -
Comparative analysis of Staphylococcus aureus strains by molecular microbiology methods
34-39Views:108Staphylococcus aureus is a very important pathogen for dairy farms and milk processing plants. Subclinical mastitis is often caused by this species, and it can contaminate bulk tank milk when milking cows are suffering from mastitis. Additionally, thermostable enterotoxins (SE) produced by some types of this bacterium can cause food poisoning.
The aim of our research was to examine the number of S. aureus in bulk tank milk in two dairy farms and the enterotoxin-producing ability, genetic relation (pulsotype) and antibiotic resistance of S. aureus strains from different sources (bulk tank milk, udder quarter milk and environment).
The results show that the mean number of S. aureus of bulk tank milk of two farms significantly differed (P<0.05). Fourteen isolates were selected for further molecular genetic studies (five isolates were from bulk tank milk and nine isolates were from udder quarter milk). S. aureus was not recovered from the environmental samples. Three of the fourteen isolates (21.4%) tested by multiplex PCR were positive for SE genes. Two isolates carried one gene (seb) and one isolate carried two genes (seg and sei). The fourteen strains were classified into three pulsotypes and two subtypes at 86% similarity level. Isolates from bulk tank milk (n=5), were divided into 2 pulsotypes (A, C) and one subtype (C1). The isolates from udder quarter milk (n=9) belonged to three different pulsotypes (A, B, C) and two subtypes (A1, C1). The distribution of pulsotypes in the present study revealed genetic relationship between S. aureus isolated from udder quarter milk and bulk tank milk. This could be explained by the fact that in farms with a high number of infected cows, these cows could represent the main source of contamination. The results of the antibiotic resistance investigations show, that all strains were susceptible to methicillin, cefoxitin, lincomycin, tetracycline, erythromycin and sulfamethoxazole/trimethoprim. Thirteen out of fourteen strains were resistant to penicillin (A and C pulsotypes, A1 and C1 subtypes) and just one isolate was susceptible (B pulsotype) to all antibiotics tested. -
How can we determine the expected prices for lambs? – Analysis of the Hungarian and Italian lamb prices
59-67Views:65The Hungarian sheep sector has become a one-market sector, almost the whole amount of slaughter lamb went to Italy, which increased its defenselessness. In addition to the sole Italian market showing a permanent demand for Hungarian lambs, it would worth to exploit possibilities in other European markets considering the seasonal differences in Italian prices. Such markets can be the Spanish and Greek for ”light” and the French, German and English markets for ”heavy” lambs. Both the Italian and Hungarian prices have three major periods: Easter, Ferragusto and Christmas. Due to the changes in the prices, the producers often suffer great losses, therefore, the temporal adaptation of production, market research, technological and development activities need to be improved. It is important that the living of Hungarian sheep breeders should not be dependent solely upon the Italian market, distribution of products should be ensured in other markets too, so that the safety of sales be increased and higher prices and higher income could be achieved. The European lamb prices are characterized by large seasonal fluctuation and the degree and timing of changes are different in the different countries. Study of the literature and the results obtained in my study on lamb sales called for an analysis and study of the possibilities of price forecasting. In my study, I performed a forecasting of lamb prices in Hungary and Italy for the period between 1996 and 2006 based on the data of the European Committee. Among the forecasting methods, seasonal decomposition and SARIMA models are the most precise, producers can achieve a better market position by using these in the practice.
-
Applicability of precision weed detection technologies
163-168Views:157In an agricultural field or horticultural plantation, weeds compete with cultivated plants for water and nutrients. The transpirated water by the weeds is needed to be replaced, which saddles surplus costs on the farmer, which could reduce the profitability of crop production. The aim of the precision plant protection system is to protect cultivated plants by applying site-specific technologies and optimized herbicides combination and methods, without environmental damage. The first step of precision weed control is the scouting for weeds. Traditional and modern (passive and active remote sensing) weed surveying technologies are available to detect weeds. The examination was carried out in an intensive apple orchard with drip irrigation system, protected by hail net of the Study and Regional Research Farm of the University of Debrecen near Pallag. The spectral-based weed detection was worked out by the Tetracam ADC broadband multispectral camera and the GreenSeeker 505 vegetation indexmeter. A strong correlation observed between vegetation indices and weed coverage. Based on the collected data, weed maps are created in appropriate software environment, thus the spatial distributions of the weeds are determined. The species level discrimination and the recognition of weed structural parameters were executed based on the 3D point cloud data by Leica ScanStation C10 laser scanner.
-
An Efficient Method for Making an Educational Program
3-8Views:58Distance education, a new teaching method widely-spread in higher education nowadays, requires the extent use of multimedia self-study educational programs besides the traditional means and methods.
Experience shows that besides certain types of software like encyclopedias, dictionaries, language manuals etc. there is an ever growing demand for appropriate educational programs that can be tailor made, adapted or modified for various kinds of students.
These programs reflect the teacher’s approach to the subject and contain the relevant, up-to-date information.
Since they are used in a relatively limited circle, usually within a faculty of an institution or within a single subject area, it is crucially important to be able to produce them with as little energy and time as possible.
There is a PhD research program, aiming at creating a flexible multimedia framework, that can be a possible solution to the problem. This framework can be easily filled with different teaching materials compiled by certain teachers without advanced knowledge of informatics. The framework itself is carefully designed and guarantees high quality. -
Surveys of residents’ attitudes to tourism development
220-227Views:92Tourism has an important role in the various development plans and strategies around the world. It has also become an important component of rural development programs, as rural communities experiencing serious economic downturns often consider tourism to be a possible way of development. The expected positive economic and social impacts may not occur and negative tendencies are reinforced if communities do not support or even oppose tourism development. For this reason, it is highly important for planners and decision-makers to understand how the public perceives the tourism industry.
In the international literature there is a growing number of studies about residents’ attitudes toward tourism development. In the present study I review the social and economic factors that influence the attitudes of residents and communities, as well as demonstrate the typologies that were created on the basis of the results. Finally, I summarize the theoretical models that are used to interpret the results of the investigations. -
Daily behaviour of Hungarian Grey Cattle under range grazing conditions
45-48Views:156Cattle behaviour on rangeland depends on external factors, such as grass allowance and quality, temperature, net solar radiation, distance from water-source, wind speed and direction, air pressure changes and the applied breeding technology. Our research is based on previous empirical observations and modern methods to analyze the behaviour of the Hungarian Grey Cattle. Today it is crucial to use cost-effective solutions in modern beef cattle farming therefore we introduce a cost-efficient method to study and follow cattle herds. We are studying relation between traveled daily distance and air pressure. The various weather fronts influence behavioural characteristics and traveled daily distance. According to our hypothesis, the pressure-change and the wind direction has significant effect on cattle activity on pasture. As the different air masses alter the barometric conditions and unbalance the neuroendocrine system, indirectly cause relaxed or agitated behaviour
-
Búzaminták fungicid szermaradvány ellenőrzése és gombafertőzöttségének kimutatása
45-51Views:74To protect crops with chemicals against different microorganisms are very important because residues of toxins could pollute infected grains. This could have the meaning of serious danger to the human and animal health. Similarly important is knowledge of details about presence or absence of any practically applied protective compounds. Experimental studies were performed on fungicide treated cereals of the Cereal Research Non-Profit Company, Szeged. Chromatographic methods were used (GC and some GC-MS) to find low level fungicide residues, microwave-assisted isolation of ergosterol was followed by HPLC to detect possible fungal infections and a more detailed microbiological analysis completed the work. Concerning the dry year 2000, no fungicides were found in the samples (by November), the ergosterol level was found to be less than 8 ppm, meaning good quality, coupled with an extremely low level of fungal infections. This was confirmed by direct microbiological testing. In comparison with the microbiological results from 1999, a negative difference can be recognized, most probably due to the rainy weather throughout the year.
-
Quality comparison of selected apple cultivars under ULO-storage
62-75Views:79Our examination was carried out in the 103 ha apple orchard of the Kasz-Coop Ltd.. The plantation works with modern varieties standing on M9 root stock, planted in a tree density of 2500 tree/ha and treated with professional integrated technology. The date of harvest is defined by a combination of several ripening analyses methods. The orchard has its own ULO-system warehouse.
Used in the experiment were four apple varieties: ’Idared’, ’Golden Reinders’, ’Jonagold’ and ’Braeburn’.
While under ULO-storage, we took 20-25 piece samples 6 times regarding the four varieties, from which 5-5 pieces were kept under three weeks’ shelf life, measured on a weekly bases. In our research, we analysed the dynamics of the flesh firmness and the refractometric value under ULO-storage and three weeks’ shelf life, and the standard deviation dynamics of the refractometric value under ULO-storage.
Analyses were based on the following measurements: flesh firmness, refractometric value and number of mature seeds.
We looked for morphological and morphological-quality correlations.
Through these we described the dynamics of the flesh firmness and the refractometric value under ULO-storage and three weeks’ shelf life for each variety. We referred to the stability of these attributes and to the dynamics of their change. Later, with the help of these, and also considering our practical experience related to the measurements, we characterised the four varieties.
We found a positive correlation between the seed number and the flesh firmness in the case of the ‘Idared’, ‘Jonagold’ and ‘Braeburn’ varieties, but no correlation was found in the case of ‘Golden Reinders’ in this concern. We visualized the standard deviation dynamics of the refractometric values under ULO-storage. This allowed us to categorise the varieties.