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Effect of floor type and bedding system on sows lifespan
155-160Views:306The paper studies two nucleus pig farms in the Great Plain region of Hungary which have same management, same feeding system but different breeding technology (Farm A had solid floor with straw and Farm B had slatted floor without straw). The genetics of sows were crossbred Dutch Large White and Dutch Landrace. The comparative examination was based on the causes of culling according to the different floor type. To the analysis of culling reasons was applied the methods of survival analysis.
In the analysis there were used the Kaplan-Meier method and the Cox Proportional Hazard model to the estimate the difference between the farms. The results of survival analysis showed significant difference (p<0.05) between the lifespan of sows that caused the difference between the survival probabilities of sows culled due to lameness. Furthermore there was showed that the sows kept on solid floor with straw have stayed in production longer than on slatted floor without straw. The hazard ratio was 1.434 that means that the culling due to lameness is 1.434 times higher by the slatted floor without straw facing by solid floor with straw.
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Application of mycorrhizae and rhizobacteria inoculations in the cultivation of processing tomato under water shortage
111-118Views:406The effect of mycorrhizal fungi and plant growth promoting rhizobacteria on some physiological properties, yield and soluble solid content (Brix) of ‘Uno Rosso’ F1 processing tomato was studied under water scarcity. Inoculation was performed with mycorrhizal fungi (M) and rhizobacteria preparation (PH) at sowing (M1, PH1) and sowing + planting (M2, PH2). The treated and untreated plants were grown with regular irrigation (RI = ET100%), with deficit irrigation (DI = ET50%) and without irrigation (I0). In drought, the canopy temperature of plants inoculated with arbuscular mycorrhizal fungi (M1, M2) decreased significantly, however, the decrease was small in those treated with the bacterium (PH1, PH2), while the SPAD value of the leaves of plants treated only with Phylazonit increased significantly. On two occasions, inoculations (M2, PH2) significantly increased the total yield and marketable yield, however, under water deficiency, a higher rate of green yield was detected than untreated plants. In dry year using deficit irrigation, the one-time inoculation (M1, PH1) provided a more favorable Brix value, while the double treatments reduced the Brix. In moderate water scarcity, the use of mycorrhizal inoculation (M2) is preferable, while under weak water stress, the use of rhizobacteria inoculation (PH2) is more favorable.
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Comparison of RAPD and AFLP Analysis in Some Maize (Zea mays L.) Lines and Hybrids
3-7Views:239The use of molecular markers to enhance plant breeding efforts is being widely studied. DNA-based fingerprinting technologies (RAPD and AFLP) have proven useful in genetic similarity studies. We estimated different maize (Zea mays L.) inbred lines and hybrids originated from mutant ones based on their genetic differences.
We carried out RAPD analysis with different primers and the 707 (CCCAACACCC) and 792 (CAACCCACAC) primers with 50% similarities provided quite good DNA fragments. By applying the DNA based-AFLP technique, we had very dense DNA fingerprinting. We differentiated 15-32 polymorphic bands, the highest number of bands were found in P-T/H-CA (32). AFLP seems to be the more efficient method of comparing genetic similarities/differences among different genotypes. -
Identification of Hucul mare families by mtDNA markers
75-79Views:620Hundred animal species have disappeared during the last century. By this time, approximately one-third of domestic animals have been in the endangered category. Hucul horses are also in this category; furthermore saving the genetic diversity beside the race preservation is an important challenge as well. The number of mares and stallions is only one of the expressive elements of genetic diversity; together with their quality determine the genetic variability of this breed. Beyond that, if an exact breed can originates from more founders, it can be more renewed genetically. Stud book documents these data by registering the mare families and stallions’ genealogical lineage. Molecular genetics, especially mitochondrial DNA analysis can make the precise identification of mare families possible. As a result of these molecular based methods, protection of genetic diversity, as well as breed preservation became more reliable. After the primer designing, the optimal primer pair was chosen which targets a 1092 bp length DNA sequence in the cytochrome b region. After the successful PCR optimalisation, we determined 170 Hucul mares’ sequences. According to our results, the samples compose ten haplotypes, which are much less, than the registered number of mare families in the stud book. Further investigations are needed to reach more representative results, and drawn the further consequences.
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The effect of β-glucan, carotenoids, oligosaccharides and anthocyanins on bacteria groups of excreta in broiler chickens
15-20Views:786This study was conducted to examine the effect of natural compounds, such as β-glucan, carotenoids, oligosaccharides, and anthocyanins in the diet on bacteria gropus of excreta in Ross 308 broiler chickens. Chickens were fed 5 diets: control (basal) diet, a diet supplemented by β-glucan at 0.05%, and diets supplemented by carotenoids, oligosaccharides, or anthocyanins at 0.5% of each compound. On experimental day 19, excreta were collected to determine the proportion of Lactobacillus, Bifidobacterium, Campylobacter, Clostridium, and Escherichia coli. Samples were collected aseptically and snap-frozen in liquid nitrogen. Bacterial DNA was isolated from samples, then polymerase chain reaction using primer pairs designed to the 16S rDNA of bacterial groups were applied to define the proportion of the mentioned bacteria. Another universal primer pair was used to amplify a region of 16S rDNA of all the examined bacteria. Proportion of each bacterial groups was determined relatively to the intensity of universal PCR product band by gel documenting system and ImageLab software. Based on the results, carotenoids and anthocyanins increased the proportion of Bifidobacterium, which might imply the beneficial effects of the mentioned compounds on the bacteria composition of excreta.
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Milestones in the development of agronomic management practices in crop production
203-209Views:282From the dawn of the history of the human race, agriculture has always been a profound activity of mankind producing food and feed as well as various plant originated materials for further processing.
Agronomy, like any other human activity, depends on the perpetual development of knowledge and technical skills, - in a modern context – science and innovation. This paper is intended to provide the reader with information regarding the main phases of the development of agricultural production from the Neolithic societies through the early Mesopotamian and Egyptian empires to the inventions of first organised learned society of Rome. The major research findings of the past two millennia including agro-chemistry, genetics and technical development are presented.
Such a review should not lead to any scientific conclusions, but rather a philosophical postulate similar to that of Jonathan Swift written some centuries ago: “And he gave it for his opinion, that whoever could make two ears of corn, or two blades of grass, to grow upon a spot of ground, where only one grew before, would deserve better of mankind, and do more essential service to his country, than the whole race of politicians put together”.