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DHAC-induced transgene overexpression in 35S-gshI GMO poplar (Populus × canescens)
78-83Views:37Relative gene expression levels of transgene gshI (γ-glutamylcysteine synthetase cloned from E. coli) were analyzed by qRT-PCR in two transgenic poplar (Populus × canescens) clones (11ggs and 6lgl) and wild type (WT). An extremely high expression level of transgene gshI was observed in the 6lgl clone (13.5-fold) compared to 11ggs (1.0) samples, which level was doubled (1.8-fold) in the DHAC (5.6-dihydro-5'-azacytidine hydrochloride) treated (at 10-4 M for 7 days) 6lgl samples but not in the 11ggs clone (0.4-fold). Contrary to this result, relative copy number of transgene gshI in the 6lgl clone was found to be less 60% less (1.0) then in the 11ggs samples (1.6). Relative expression levels of proper poplar gene gsh1-poplar showed significantly higher responsiveness to DHAC treatment than transgene gshI with the highest expression level in the untransformed (WT) poplar
clone (19.7-fold) compared to transformed 6lgl (8.7-fold) and 11ggs (2.5-fold) clones. For internal controls constitutively expressed housekeeping genes a-tubulin were applied. For data analysis, the 2−ΔΔCt method was used. DHAC applied in long-term cultures (27 days) at low concentrations (10-8 – 10-6 M) showed morphogenetic activity by initiating de novo root development of leaf discs. -
How can we determine the expected prices for lambs? – Analysis of the Hungarian and Italian lamb prices
59-67Views:70The Hungarian sheep sector has become a one-market sector, almost the whole amount of slaughter lamb went to Italy, which increased its defenselessness. In addition to the sole Italian market showing a permanent demand for Hungarian lambs, it would worth to exploit possibilities in other European markets considering the seasonal differences in Italian prices. Such markets can be the Spanish and Greek for ”light” and the French, German and English markets for ”heavy” lambs. Both the Italian and Hungarian prices have three major periods: Easter, Ferragusto and Christmas. Due to the changes in the prices, the producers often suffer great losses, therefore, the temporal adaptation of production, market research, technological and development activities need to be improved. It is important that the living of Hungarian sheep breeders should not be dependent solely upon the Italian market, distribution of products should be ensured in other markets too, so that the safety of sales be increased and higher prices and higher income could be achieved. The European lamb prices are characterized by large seasonal fluctuation and the degree and timing of changes are different in the different countries. Study of the literature and the results obtained in my study on lamb sales called for an analysis and study of the possibilities of price forecasting. In my study, I performed a forecasting of lamb prices in Hungary and Italy for the period between 1996 and 2006 based on the data of the European Committee. Among the forecasting methods, seasonal decomposition and SARIMA models are the most precise, producers can achieve a better market position by using these in the practice.
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The possibilities of the development of functional dairy products
33-36Views:150Because of our new aged world’s emerging stress, over exhausting and move less lifestyle the pollution, the not appropriate food consuming and the low calories in the consumed food there are more and more the called „civilian” disorders.
Many people have diabetes and osteoporosis others fight with lung, cardiovascular system, problems and find cancers of many kinds without age exemptions.
There could be a solution in changing lifestyle. In the developed side of the world there are presence of food lines with higher content in nutritious and/or vitamins and fibers such as: kalium, calcium, selen, magnesium, plus it contains less saturated fat. Because of that there is a new word in dietetics as functional foods.
Food having more inner content and/or biological values, so healthier, are called functional foods. Those foods components picked carefully for healthiness by modern knowledge of dietetics.
One of the best raw materials for functional food is the milk. It is already healthy by itself. The Körös-Maros Biofarm Ltd. has a goal of developing and marketing, health protecting organic functional foods in hungaricum products from cured milk. The Jedlik Ányos project helps the Ltd. to achieve this goal. We just finished an antioxidant test and we check if the antioxidant in present in the milk than we check the final product of the presence of the original antioxidant and the form and amount of it.
It had been feeding, for two weeks for three herd of cows selected for age, milk output and consanguinity. All of the three herd had been feeding with basic forage. One of the herds had been getting vitamin-E in the amount of 250 mg/bwkg the other group got licopin in the amount of 200 mg/bwkg once a day each by each orally, the third herd was the control group.
After two weeks we toke a sample of every herd were processed the samples into yogurt and cheese. Than we checked the raw milk, the cheese and the yogurt for antioxidant content with HPLC method. The test ended with good results by finding a great amount of antioxidant, in not only the raw milk but also in the final product. -
Effect of two different cropyear and the agrotechnological factors on the agronomic characteristic of the winter weat in a lon-term experiment
143-149Views:125We studied the effects of crop rotation, fertilization and crop protection technologies on occurence of the major ear- and leaf-diseases (powdery mildew, helminthosporiosis, leaf rust, Fusarium wilt) and the degree of lodging in the winter wheat variety Mv Pálma in two very different years (2006/2007=dry; 2009/2010=rainy). The experiments were carried out at the Látókép Experimental Farm of the University of Debrecen CAAES in triculture (pea-wheat-maize) and biculture (wheat-maize) at five fertilization levels by applying three different crop protection technologies (extensive, average, intensive).
In the cropyear of 2006/2007, the disease severity of leaf diseases was higher than the average in both crop rotations in spite of the fact that the weather during the whole vegetation period was dry. Infection by powdery mildew, helminthosporiosis and leaf rust increased with increasing fertilization, the highest infection was measured at the highest fertilization level (N200+PK) in the extensive crop protection technology. According to the results, no infection of ears by Fusarium and no lodging occurred in either bi- or triculture due to the dry year. The extremely rainy weather in 2009/2010 was favourable not only for the vegetative development of the stand, but also increased the occurance of leaf-, stalk- and ear-diseases and a high degree of lodging was observed. The highest infection by powdery mildew was observed in the plots with the highest fertilizer dosage under an extensive crop protection technology. A much higher helminthosporiosis infection was measured than in the cropyear of 2006/2007. The wet weather and higher than average temperature promoted the occurence and spreading of leaf rust. Under an extensive crop protection technology, a leaf rust infection of 24% and 31% was detected after maize and pea as a forecrop, respectively, in the N200+PK treatment. As opposed to 2006/2007, the disease severity of Fusarium was 3–8% and 2–7% in the control after maize and pea as a forecrop, respectively. This value, similarly to that of other pathogens, increased with increasing fertilization levels. Due to the large vegetative mass, a significant lodging was observed in the wheat stands in both bi- and triculture (17–100%, 12–100%). -
Computer simulation modeling of Leaf Area Index (LAI) in maize
5-8Views:142This study presents a PHP-based model capable of calculating maize leaf area index. The model calculates LAI from emergence to 75% silking. The basis of calculation is represented by the daily average temperature values. The usability of the model was tested using three years' temperature and LAI data series from the values obtained by the weather station set up at the Látókép Experiment Site of the University of Debrecen, Centre for Agricultural Sciences between 1994 and 1996. During the running of the model, it was observed that temperature affects the intensity of leaf development to a various extent.
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Biomethane production monitoring and data analysis based on the practical operation experiences of an innovative power-to-gas benchscale prototype
399-410Views:369Power-to-gas (P2G) is referred to technologies that convert carbon dioxide into methane. Both bio- and chemical catalysts may be used for conversion purposes. One of the most disruptive biotechnologies was developed by the University of Chicago (IL) (publication number: EP2661511B1), using a robust, highly selective, patented strain of Archaea. Electrochaea GmbH has developed an innovative bench-scale P2G prototype unit, which uses this highly efficient Archaea strain, specialized components and specifically developed control strategies. The structure and the components of the prototype are equivalent with the functional parts of the currently largest commercial scale biomethanation BioCat plant located in Avedøre, Denmark (www.biocat-project.com). Power-to-Gas Hungary Kft. has committed to further develop this innovative technology. The first steps of this development have been taken by operating the benchscale unit and analyzing the data of the operating periods.
The prototype is operated based on weekly campaigns. During continuous operation, H2O is generated as a by-product of methane. Therefore, approximately 200 ml of biocatalyst is discharged each day and concentrated media containing macro and micronutrients are injected into the reactor to maintain media composition. The laboratory staff records all gas composition data each morning. The gas composition is measured every 12 minutes by an Awite AwiFlex Cool+ gas analyzer. Within this article, we analyze the collected datasets containing more than 12 000 records and present the first practical experiences of the operations of the innovative power-to-gas bench-scale prototype.
The analysis of the collected gas composition data of the product gas already provides important data for modelling the commercial-scaled processes. The average value of VVD was about 40 l/l/d in the period under review. Further increase of the methane content can be achieved by introduction of higher mixing energy and by increasing pressure levels in the bioreactor (as demonstrated in the BioCat plant – data not shown here) – both of which are strategies envisioned for the commercial plant. In routine activities (turn on, shut down, continuous operation) we could verify the high robustness of the biocatalyst and the base connection between the registered datasets and performed test results.
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Nutrient deficiency and effects of various nutrition technologies on crop health
109-113Views:241The impacts of climate change on crop production are increasingly noticeable. Extreme weather conditions – such as devastating droughts, which occur more often – have serious effects on crop conditions, thus damaging their defence ability against pathogens and pests. Therefore, in order to achieve high-quality and high yielding crops, it is urgent to elaborate new technologies that improve general condition of crops and prevent development of nutrient diseases. Those crops which suffer from the lack of certain nutrients are more sensitive and their tolerance against diseases are decreased. Nitrogen – as the most influencing macronutrient in yield – is also essential in maintaining crop health. Nevertheless, due to the complicated processes in soil (such as leaching, denitrification), the utilization of nitrogen is not nearly complete, therefore nitrogen stabilizers may be needed to maximize this factor. The use of these stabilizers can be promising where plants with high nitrogen content are grown, although further experiments are needed in which impacts of nitrogen stabilizers on crop protection aspects are examined as well, since there is a close correlation between exaggerated nitrogen fertilizing and sensitivity to pests. During my research I am going to examine the combined effect of foliar fertilizer and nitrogen stabilizer on crop health. Furthermore, my goal is to find clear correlation between pathogens and the different technological variants of nutrition.
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The Role of Trichoderma in the Rhizosphere of Tomato Plants
67-69Views:64It is well established that microorganisms are closely associated with the roots of plants can directly influence plant growth and development. Species of Trichoderma, on the other hand are primarily studied for their ability to control plant disease. The ability of species of Trichoderma to directly promote or inhibit plant growth has been noted for many years.
Tomatoes were treated with different Trichoderma strains by seed treatment and soil inoculation. The Trichoderma were grown on malt-agar medium and conidia were washed off by sterile water for making suspension which contained 107 CFU/ml (colony forming unit/ml). The suspension was used for seed treatment and for the soil inoculation by watering as well. The artificial soil inoculation was made by Trichodermas growing on grounded maize were mixed in mould and tomato seed were sown in it. Tomato seeds were also sown in bags made of close-meshed material which allowed the soil microorganisms to colonize the roots but it simultaneously protected the roots from soil contamination. Roots were put on Trichoderma selective medium to check the root colonization of the Trichoderma.
The tomato plants were bedded out in a field in four repetition. After harvesting by hand the results supported by statistics shown that there was significant differences between the yield of the untreated and treated tomato plants by Trichoderma strains. -
Effect of Auxin-Synergistic Preparation and Fertilization on Fruit Setting and Fruit Quality ofApple
21-26Views:131On seven apple cultivars (Gala Must, Gloster, Granny Smith, Idared, Jonagold, Jonathan Csány and Mutsu), the author studied the influence of N-phenyl-phthalamic acid (Nevirol 60 WP) and fertilization on fruit setting and fruit quality.
The research results show that by using Nevirol 60 WP and applying fertilization, we can improve the fruit setting and number of fruit per tree. As a result of this process, the yield amount will increase.
Nevirol 60 WP has a great effect on the improvement of yield amount, especially by good nutrient supply. There is a significant difference between the reactions of different cultivars. The most sensitive to these treatments were the cultivar Gala Must and Granny Smith. Before using Nevirol 60 WP we have to take into consideration that the increased yield needs a higher nutrient supply; otherwise, fruit mass, diameter and fruit height will decrease, and the yield will frittered away. In the study, the most favourable effect of Nevirol 60 WP was detected under additionally fertilized conditions by the author. -
N-Phenyl-Phthalamic Acid and Fertilization Effects on Flowering, Fruit Set and Fruit Quality of Apple (Malus domestica Borkh.)
24-28Views:121On seven apple cultivars (Gala Must, Gloster, Granny Smith, Idared, Jonagold, Jonathan Csány 1, Mutsu), the authors studied the influence of N-phenyl-phthalamic (PPA) acid and fertilization on flowering, fruit set and fruit quality in the years 2003-2004. The research results showed that PPA application extended the flowering time of the most cultivars. Fruit set of apple cultivars increased in many cases when regulator was applied. The additional nutrient supply could increase the fruit set too. The increase of fruit set increased the number of fruit per tree. Quality parameters determined by fertilization and fruit number per tree. For example, when N-phenyl-phthalamic acid was applied without fertilization the fruit number per tree increased considerably however, it caused a considerable frittering away. The reason for this is that fruit set was high and fruit received not enough nutrition for growing up. The skin colour of fruits was decreased in several cases by the treatments. In one respect the enlarged fruit number was shaded the others and the fertilization increased the vegetative shading leaf area.
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Agronomical and economic evaluation of various cultivation systems on meadow soil
103-106Views:108The requirements and objectives of cultivation are in constant change. There are different cultivation aims if the objective is soil protection, the prevention of its moisture content or on areas with different precipitation supply or production site endowments. Based on the experimental database of the Institute for Land Utilisation, Regional Development and Technology of the University of Debrecen, Centre for Agricultural and Applied Economic Sciences and the KITE Plc., the various cultivation systems in Hajdú-Bihar country were examined with maize as indicator plant. The sample area can be found in the outskirts of Biharnagybajom on meadow soil. On the examined plot, spring strip basic cultivation, loosening and autumn ploughing were applied on 15-15-15 ha, respectively. At the time of taking undisturbed soil samples, soil conductivity measurements were also performed with a Penetronik penetrometer. Undisturbed soil samples were taken from each treatment before sowing (on 5th April 2012). The yield obtained in the strip cultivation treatment increased that of the ploughing and the loosening technology. The economic indexes are the most favourable in the strip cultivation.
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Renewable energy on animal farms: support system and practical application
13-17Views:152Renewable energy applications were examined in animal farms. The investment support system for animal farms – which has renewable elements – has been summarized through analyzing the legislation and future prospects. The most important in this topic is the Animal Farm Modernization Support, which had several application periods during the 2007–2013 CAP budgets. For analyzing practical application of renewable energies interviews and farm visits have done in Hajdú-Bihar County. The questions covered the general attributes of the farms and the details of the (existing or planned) renewable energy application. In terms of economic analysis saving, the investment return time and the employment effect were examined. The results show wide variety of applied renewable energy application. The investment supports plays great role in renewable energy projects by lowering the return time; and through additional points in application assessing, which is an incentive for farmers to include renewable energy element in their modernization projects.
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Herbaria and foods in the development of microregional spaces
87-89Views:178This study aims to define the concept and significance of herbarium in space and time. It compares the basic ideas of traditional and modern paradigms paying special attention to medicine and nutrition. It examines the notion and role of functional foods in the light of paradigm changes, health preservation, and healing. Concerning the improvement of microregional areas, it evaluates the possible role of herbaria in creating social cohesion, community building, education, and economy improvement.
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Characterization of two rust fungi related to biological control concept in Hungary
195-199Views:286Weeds cause serious problems in agriculture on a global scale. These plants reduce yield and the quality of crops by competing for water, nutrients and sunlight. The improper or excessive usage of herbicides have led to development of resistance in some weed species while contaminating the environment; therefore, biological control has an increasing role as an alternative method for controlling special weed species.
The aim of this study is to make a brief review of biological control of weeds by pathogens and to characterize two rust fungi (Puccinia lagenophorae and Puccinia xanthii) which are broadly examined recently in a biological control concept and have been found on their hosts, such as common groundsel (Senecio vulgaris L.) and common cocklebur (Xanthium strumarium L.), two common and difficult to manage weeds both in horticultural and agricultural lands also in Hungary.