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Studying the reproduction-biological effect of the GPR54 receptor gene in sheep
48-51Views:457One of the main economical problems of sheep breeding is that continual production is not possible due to the seasonality of animals. Today, genes, mutations that may develop aseasonality in sheep are extensively researched in order to make the establishment of populations capable of aseasonal estrus possible.
According to the current knowledge, the GPR54 gene participates in the GnRH release from the hypothalamus, and thus in the alteration of the reproduction state of the organism. Our research is aimed to determine whether the GPR54 gene has allele variations that influence the proneness to aseasonal estrus in sheep in a different extent. Therefore, the GPR54 gene sequence of sheep is first examined. -
Microbiological and Chemical Characterization of Different Composts
106-111Views:202Composting of agricultural waste is considered particularly important from the point-of-view of environmental protection. Degradation of organic substance results in a significant reduction of waste volume.
The end product of the composting process, mature compost, can be used as soil coverage against excess loss of wastes, for mulching, for organic manure etc. The problem of composting has come into limelight in environmental studies and in agriculture.
The quality of the mature compost is determined by physical, chemical and biological parameters of the composting process which, in turn, depend on initial composition of the raw materials, the technology, e.g. regular mixing and moistening and on environmental factors. Quality is the key question in compost use.
We studied the composting process in compost windrows of different raw material composition. We measured temperature, humidity content, pH, organic substance content, nitrogen and carbon content.
We counted the number of bacteria, microscopic fungy, ammonifying and cellulose decomposing microorganisms. We directed the composting process with turning weekly (to provide oxygen) and watering (to provide humidity content 40-60%).
We set up windrows of 1 m3 volume from dry plant substances (cornstalk, pea straw, tomato stalk and crop, weeds) and cow manure not older than 1 week. The cow manure was used at ratios of 0%, 35%, 50%, 65% and 100%, respectively.
We measured changes in compost temperature relationship with outside temperature until they were almoust the same. Humidity was 40-60% in most cases.
At the beginning of the process, pH was slightly acidic-neutral; it later becomes neutral-slightly alkaline (pH: 6.93-8.02) as ammonia is liberated from proteins.
At the end of the process, pH decreased again, due to humification.
Organic substance content decreased as microorganisms mineralized them. Organic carbon content decreased gradually due to microorganisms used it as an energy.
Total nitrogen content increased until middle of july and decreased gradually until than.
The carbon/nitrogen rate were higher in the beginning, it decreased until july-august and increased by smaller degree until end of the process.
The number of bacteria was higher in the first three weeks and between june-september. The number of cellulose degrading bacteria was the highest in the first three month, the number of ammonifying bacteria was the highest from the end of may until sepember.
The number of microscopic fungy was significant in the second part of process, after july. -
The Biology, Ecology and Medicine Faculty of Dnipropetrovsk National University after Oles’ Gonchar
15-17Views:218The historical rewiev of Biology, Ecology and Medicine Faculty is presented. The Faculty of Biology, Ecology and Medicine has 7 Departments, Aquarium complex, Zoological Museum, Vivarium and Herbarium. It works in cooperation with the Research Institute of Biology, Botanical Garden, O. L. Bel’gard International Biosphere Station, and Biological Station of DNU and forms the regional Centre of Science, Education and Culture in the field of Biology, Ecology and Nature Conservation in Central Ukraine. The Faculty proposes courses in the following specialities: Biology, Zoology, Botany, Microbiology and Virology, Biochemistry, Physiology, Ecology, Environmental Protection and Balanced Nature Management. All of them have the highest IV level of accreditation. Students get a pedagogical education.
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Studies of plant density increase – on maize hybrids of various genotypes on chernozem soil
87-92Views:617The yield and crop safety of maize are influenced by numerous ecological, biological and agrotechnical factors. It is of special importance to study one of the agrotechnical elements, the plant density of maize hybrids, which is influenced by the growing area conditions and the selected hybrid.
We have investigated the effects of three different plant numbers (50 thousand plants ha-1, 70 thousand plants ha-1 and 90 thousand plants ha-1) on the yield of 12 maize hybrids of different genotypes in Hajdúság, on calcareous chernozem soil, in the Látókép Research Farm of the University of Debrecen, Centre for Agricultural Sciences, in 2013. The experiment was set in four replications, besides commonly applied agrotechnical actions. In the experiment, 1 hybrid of very early (Sarolta), 9 of early (P 9578, DKC 4014, DKC 4025, P 9175, NK Lucius, Reseda, P 37N01, DKC 4490, P 9494) and 2 of medium (Kenéz, SY Afinity) maturation were used.
With the increase of the plant number, the number of individuals per unit area increases. According to our experimental results, we have concluded that with the increase of the plant number, the yield increased in the average of the hybrids. In the average of the hybrids, in the case of 50 thousand plants ha-1, the yield was 13 130 kg ha-1, in the case of 70 thousand plants ha-1, it was 13 824 kg ha-1, while in the case of 90 thousand plants ha-1, the yield became 13 877 kg ha-1.
In addition to plant density increase, it is necessary to determine the optimal plant number that is the most favourable for the certain hybrid under the given conditions. To fulfil this aim, we have determined the optimal plant number corresponding to the maximum yield of the given hybrid, within the given plant number range. The optimal and applied plant numbers differ, since the optimal one could only be applied under ideal conditions. Since the agrotechnical actions cannot always be carried out in appropriate quality and one has to adapt to the weather conditions, thus we have determined a plant number range in the case of each hybrid. The hybrids were classified into categories of producible in narrow and broad plant number range.
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Investigating the above-ground biomass values of sweet potatoes (Ipomoea batatas)
35-39Views:477The role of sweet potato tubers in human nutrition is not new. The above-ground biomass of sweet potatoes is not used for nutritional purposes in most countries, but it has a high biological value. Therefore, the aim of the present study is to investigate the production of press fibre from above ground biomass by wet fractionation. Two sweet potato varieties (purple- and white-fleshed sweet potato) and two types of irrigation system were used: bubbling water flow system (BWS) and continuous water flow system (CWS). Glucan, xylan, arabinan were analysed by HPLC and elemental content was measured by ICP-OES. Our results show that the total carbohydrate content in the pressed fibre of the leaf blades (27.64–29.88 %w/w) is lower than in the stem with petiole (51.14–57.36 %w/w). No significant difference in glucan, xylan and arabinan content was observed in the leaf blade. In the stem with petiole, significant differences were observed for xylan and arabinan contents. For elemental content, generally higher values were measured in the leaf blade than in the stem with petiole. This information may be relevant for the selection of the appropriate variety and treatment, even for the production of functional food.
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Comparison of Reproductive Performance of the Roe Deer (Capreolus capreolus, L.) Among Different Regions
41-46Views:264The potential and actual number of offspring of roe deer and the difference between these figures (prenatal and postnatal loss) significantly vary in each population yearly. The objective of this study is to examine the potential and actual number of offspring, the number of losses, and to find a link between the most important biological characteristics of does (body weight – BW, condition – KFI) and the number of raised offspring on four territories on the Great Hungarian Plain.
Where the number of corpora lutea (CL) is the highest, there the losses are the highest as well, and the number of raised offspring is the lowest (region I.). Here, the rearing loss is double that of the weakest territory (region IV.). Rearing losses can be associated with the fenotype of does (BW, KFI) but environmental factors also have determinative importance. Where the number of twin-calving does was the highest, I found four times more does without a fawn than where the number of twin-calving does was the lowest. The nursing success was the best (the losses were lowest) in the region where the potential offspring (number of CL) was also the lowest, but the coverage of the habitat and the proportion of forests were largest. The food supply for the animals in autumn and winter are not enough, the structure of the habitat has to be improved as well, so that it might become adequate for game protection in extreme weather conditions.
The results have to be considered as preliminary ones. It is essential to continue and extend the research to increase the reliability of the results. -
Preliminary estimation of the efficacy of Fusarium sporotrichioides Sherb. as biological control agent against common milkweed (Asclepias syriaca L.)
201-204Views:670A study of fungi responsible for severe leaf spots of common milkweed (Asclepias syriaca L.) in the Hajdúság region (East Hungary), Fusarium sporotrichioides and Alternaria alternata were isolated from infested leaf tissues. F. sporotrichioides was the most virulent fungus in pathogenicity tests conducted on healthy leaves of common milkweed plants. Inoculation of common milkweed (A. syriaca) in different growth stages with F. sporotrichioides yielded similar symptoms as the original ones. Spray mixtures containing 1.0×106 conidia/ml gave effective control when common milkweed plants were sprayed until runoff occurred. Laboratory (wet chamber) and field experiments showed that asexual spores of the fungal pathogen, F. sporotrichioides, exhibited bioherbicidal activity against common milkweed (A. syriaca).
More efficient control efficacy was observable on elder plants (at flowering stage) than younger ones. These results initiate that this fungus may be a biocontrol agent for controlling this invasive weed but should clarify its hosts because it could infect cultivated plants as well.
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The effect of lead and copper heavy metal salts on soil microorganisms under laboratory circumstances
55-59Views:446he population dynamics of calcareous chernozem soils polluted with different concentrations of lead and copper heavy metal saline solutions was examined.
The experiment was carried out in the soil biological laboratory of the Institute of Agricultiral Chemistry and Soil Science at DE AGTC MÉK in 2012. For the determination of the concentration of the undiluted stock solutions we multiplied the smallest toxic concentration values of the MSZ 08-1721/1-86 Hungarian standard by forty. The intermediary concentrations of the treatments were produced with adequate dilution of the stock solutions until a dilution level equal to the values of the standard. The statistical evaluation of the data was performed with ANOVA (Analysis of Variance) including the determination of the standard deviation and significant difference. Investigating the effects of the different treatments on the soil microbes we established that both heavy metal saline solutions had a negative effect on the population dynamics of bacteria and microscopic fungi living in the soils. The negative effect of copper – as a potential toxic micro nutrient – turned out to be less strong than the negative effect of the toxic lead. According to our results the correction of the treatment levels is recommended in order to further tolerance examinations and the determination of the tolerance levels.
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Balancing chemical fertiliser application for optimal maize yield and environmental safety
65-76Views:247Chemical fertilisers play a crucial role in enhancing maize productivity by supplying essential nutrients, particularly nitrogen, phosphorus, and potassium. However, excessive and imbalanced application of these fertilisers may result in soil degradation and environmental pollution. This review presents a comprehensive, bibliometric, and literature-based analysis of research on the balanced use of chemical fertilisers in maize production, spanning 2000 to 2025, and uses VOSviewer 1.6.20 as the analytical tool. The study analysed data from the Web of Science to examine publication trends, the configuration of keyword networks, and the dynamics of international research collaborations. The Mann-Kendall test revealed a significant positive trend (p = 0.001) in the volume of publications focused on chemical fertiliser balancing, with a coefficient of determination (R²) of 0.5153. A total of 549 relevant studies were identified globally, with no language restrictions applied, indicating sustained growth in research output over time. Field studies demonstrate that reducing chemical fertiliser use by up to 25% when combined with biological amendments can maintain or enhance yields across diverse agroecological conditions. The emphasis is placed on sustainable fertilisation practices that balance productivity and environmental safety, highlighting challenges and future directions for adoption. Balanced fertiliser management is crucial for sustainable maize production systems, which simultaneously contribute to food security and environmental conservation. This study is significant as it systematically maps the evolution and current state of research on balanced chemical fertiliser use in maize production, providing valuable insights into publication trends, collaborative networks, and practical agronomic outcomes. Despite the progress, there remains a significant gap in region-specific guidelines and long-term impact assessments of integrated fertiliser management practices, which future research should address to optimise sustainable maize production tailored to diverse agro-ecological zones.
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Introduction of DNA-based Methods to Agriculture Through Molecular Taxonomic Examination of Poa Species
139-142Views:443biological methods, one among them is AFLP that is well applicable for taxonomic research. Bluegrass species, that are important components of meadow associations, thus their thorough knowledge is necessary in maintaining biodiversity, were examined with bringing this method to perfection.
Taxonomic relationship of the members of Poa pratensis aggregation is a controversial issue. Present study aimes to identify the members of this group, with a developed AFLP method through molecular taxonomic examination of Poa species in meadows nearby Debrecen, revealing their genetical distances. Species of the aggregation show a great genetic variability, but their genetic proximity approves the use of the term aggregation. Results established wider geographical investigation of three species of the aggregation. The distinctness of the species based on their morphological features was confirmed according to their genetical basis as well. The method overall turned out to be appropriate for the taxonomic research of bluegrass species like Poa pratensis and its aggregation. -
Fusarium culmorum isolated from rhizosphere of wooly cupgrass (Eriochloa villosa) in Debrecen (East Hungary)
93-96Views:364Wooly cupgrass (Eriochloa villosa) is an East-Asian originated weed species and it has been spreaded worldwide by now. The first occurrence of this species in Hungary was observed and published in 2008 nearby Gesztely village (Borsod-Abaúj-Zemplén county, North-East Hungary) than in the summer of 2011 a significant population was discovered next to Debrecen city (Hajdú-Bihar county, East Hungary).
In 2013 this weed was also reported from Szentborbás village, Somogy county (South-West Hungary). These observations of spreading and its biological features (production of stolons and large number of seeds, moreover herbicide tolerance) indicate that wooly cupgrass (E. villosa) has a great potential of invasiveness, so it may become a hazardous weed not only in Hungary but in all over the world.
The objective of this study was to identify the fungus which was isolated from wooly cupgrass (E. villosa) root residue samples which were collected after maize harvesting on arable land in late autumn, near Debrecen. The identification of the fungus based on morphological characters of colonies and the features of conidia developed on potato dextrose agar (PDA) plates. After the examination of axenic culture we revealed that the fungus from rhizosphere of wooly cupgrass was Fusarium culmorum. Pathogenicity and/or endophytic relationship between the fungus and wooly cupgrass is still uncertain so pathogenicity tests and reisolations from plants are in progress.
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Effect of plant extract against opportunist human pathogen soil bacteria
89-93Views:256Our experiments have repeatedly shown that the extract of ragweed (Ambrosia artemisiifolia) is an antifungal and antibacterial component.
In our paper we tested of the biological activity of the herbal substance against opportunistic human pathogenic bacteria strains (Staphylococcus
aureus 110003 and 25923; Staphylococcus saprophyticus 110008). Our laboratory tests show that the extract is bacteriostatic and in several cases bactericid. We can assume that from the the agricultural and public health aspects can be extremely dangerous weed, contains biologically active components and it may be suitable for the prepare of antibacterial agents. -
The effect of various composts on vegetable green mass on two soil types
179-183Views:512Composting of sewage sludges makes easier the utilization of sewage sludge in the agriculture and the composts in good quality could increase the nutrient content of soil. Due to the composting process, the sewage sludge composts with high organic matter content can be utilized in the same way as other composts or farmyard manure.
Composts produced in different ways have different effects on the physical, chemical and biological properties of different soils, although their positive effects have already proved in the literature. In our study the effects of composts from different composting processes were investigated in soil-plant systems. The different physical and chemical properties of the two examined soil types (arenosol and chernozem)strongly influenced the nutrient supply capacity of composts which could be characterized by the growth of ray-grass as a test plant in the pot experiment. In this work we examined the effects of three different composts on the green weight of plants on the fourth and eighth weeks after the treatment and sowing.