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Phosphorus (from Different Phosphorus Sources) Utilisation in Piglets, and the Effect of the Addition of Phytase into the Feedstuff
30-36Views:245The experiments were carried out in a 2x2 factorial treatments with three replicates, and were completed with 32P phosphorus metabolism measurement. Hungarian Large White x Dutch Landrace growing pigs with 15–18 kg starting live weight were involved in the experiment.
The experimental scheme was the following:
Diet consisted of maize and extracted soybean meal. Both components have high phytase content and low phytase activity. 1/a animals received their P-supply according to their needs and 1/b animals got 10% less than their actual P-need in the first part of the experiment.
In the second part of the experiment both groups (2/a, 2/b) received identical P-supply and 500FTU/kg P supplementation. Apart from P- and phytase-supplementation, the piglets’ diet was identical.
Total P digestibility was 52% without phytase supplementation, which increases by 4% when P was added according to need and by 12% increase of decreased P-supply. Digestibility of nutrients somewhat increased as effect of phytase supplementation. According to the results of 32P experiments, inorganic P digestibility of MCP was 82–90.8%, which decreases to 73.4–87.2% in case of phytase supplementation.
Parallel with tendency, native P digestibility of the diet was 31.5–32.2%, which increased to 42.5–54.5% in the case of phytase supplementation.
Results support the that inorganic P input can be decreased by phytase supplementation and as a consequence P output, the concept and environmental pollution can at the some time be decreased. -
Storage of ram semen in gelatin supplemented extender
7-10Views:374The aim of our study was to examine how different gelatin concentrations affect ram semens viability in liquid storage at 5 oC for five days. Our hypothesis was if we add gelatin to the semen extender, than the viability of ram semen will be better in the extenders containing gelatin, than the control. We used two different semen extenders:1.5% UHT milk and 1.5% UHT milk + 5% egg yolk. We added 0; 0.5; 1.0; 1.5; 2.0% Dr. Oetker gelatin to the semen extenders. We stored the semen for five days at 5 oC and in every 24 hour we made sampling.
We stained the smears with Kovács-Foote staining and evaluated them with light-microscope. We categorized the cells in five groups like: live and intact cells, live cells with injured acrosome, dead cells, live head with dead tail and live tail with dead head. We used one-way analysis of variance (ANOVA) to assign how gelatin concentration affects the number of the categorized cells. On the fifth day, the viability was the best in the following semen extenders: 1.5% fat UHT milk + 1.0% gelatin and 1.5% fat UHT milk + 1.5% gelatin, but it was not significant (p>0.05).