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• Reference genes for livestock gene expression profiling – Literature review

  81-89

  Ádám Simon

  Zsuzsa Zakarné Aszalós

  András Jávor

  Levente Czeglédi

  Views:

  195

  Quantitative real-time polymerase chain reaction (qPCR) is an essential tool for understanding animal cell’s response to developmental progression or to different experimental conditions at gene expression level. However the reliability of this method heavily lies on proper normalization (measuring a target and a reference gene’s expression from the same sample to correct for technical related variations).
  Our literature review aimed to summarize the articles addressing the most important livestock species in regards of reference gene stability used as normalizers for quantitative real-time polymerase chain reaction experiments. Stably expressing reference genes were categorized into 14 distinct groups according to gene function. The number of reference genes tested and the publication numbers according to years and the ranking algorithms were also noted.
  Counting showed that genes encoding ribosomal protein components are ranked as most stable in majority of cases and therefore should be taking into account for qPCR stable normalizer gene finding experiments.

• Nutritional disorders of arable crop growth in eastern Croatia

  273-290

  Vlado Kovačević

  Views:

  154

  Nutritional imbalances accompanied with growth retardation of crops at early growth stage were found since the last 40 years on certain arable lands in eastern Croatia. In this regard, phosphorus (P) deficiencies in maize and wheat were found mainly on acid soils of the western part of the region, potassium (K) deficiencies in maize, soybean on the hydromorphic neutral to alkaline calcaric drained gleysols of Sava valley lowland, while zinc (Zn) deficiencies were observed mainly in seed-maize and soybean on neutral calcaric eutric cambisols of the eastern part of the region. Cold and moist spring is factor promoting P deficiency symptoms. As oasis of normal crops existed on same arable land, comparison of plant and soil composition was possible from typical sites. P nutrition disorders were in connection with the lower P and the higher aluminum (Al) and iron (Fe) concentrations in the top of plants and the lower soil pH values. K-deficiency as result of strong K fixation and imbalances with high levels of magnesium (Mg) were the main responsible factors of low maize and soybean yields on some drained gleysols. Chlorosis incidences typical for Zn deficiency in maize and soybean were in close connection with the higher soil pH, the lower quantities of mobile Zn, here and there the higher mobile P in soil, the lower concentrations of Zn and the higher levels of Al and Fe in plants. Overcoming the above mentioned disorders and normalization of yields were achieved using ameliorative fertilization either by K or P fertilizers and in case of Zn by foliar spraying of crops with 0.75% ZnSO4 solution. Also, alleviations are possible by selection of more tolerant genotypes of field crops to specific types of nutritional disorders. From this aspect, some practical solutions were recommended for maize with reference to K nutritional problems.