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Investigation of combining ability and superiority percentages for yield and some related traits in yellow maize using line × tester analysis
Published May 20, 2020
5-14

Combining ability estimation is an important genetic attribute for maize breeders in anticipating improvement in productivity via hybridization and selection. This research was carried out to investigate the genetic structure of the 27 F1 maize hybrids established from nine lines derived from Maize Research Department and three t...esters, to determine general combining ability (GCA), determine crosses showing specific combining ability (SCA) and superiority percentages for crosses. Nine lines, three testers, 27 F1 hybrids and two check commercial hybrids (SC162 and SC168) were studied in randomized complete block Design (RCBD) with three replications during 2016. The results of mean squares showed that significant and highly significant for most studied traits (days to 50% tasseling, days to 50% silking, plant and ear height, ear position, ear length, no. of kernels per row, 100-kernel weight and Grain yield). Estimates of variance due to GCA and SCA and their ratio revealed predominantly non-additive gene effects for all studied traits. Lines with the best GCA effects were: P2 (line 11) and P6 (line 21) for grain yield, for testers Gm174 and Gm1021 had significant GCA effects for grain yield. The hybrids P5×Gm1021, P6×Gm1021, P7×Gm1021, P8×Gm1002, P9×Gm1002 had significant and negative SCA effects for grain yield. Crosses P1×Gm174, P2×Gm1002, P5×Gm1021, P6×Gm174, P6×Gm1021, P7×Gm1021, P8×Gm1002, P9×Gm1021 were the best combinations manifested and significant superiority percentages over than check varieties (SC162 and SC168) for most studied traits. Therefore, these hybrids may be preferred for hybrid crop development.

Abbreviations: GCA general combining ability; SCA specific combining ability

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Development of an InDel marker set to establish hybridization between wild boar and domestic pig (Sus scrofa) breeds
Published May 23, 2019
21-25

Wild boar and domestic pig breeds belong to the same species (Sus scrofa), so they can easily have viable offspring. This could be a problem in preserving the genetic lines of wild boars, keeping clean the food industry from lower-grade hybrid boar meat, and „producing” ethically questionable trophies, too. The aim of our study was to devel...op a cost-efficient, fast, easy and accurate marker set which can separate the wild boars from hybrids and domestic pig breeds.

The InDel markers were developed using 59 full pig genomes of 17 different breeds (e.g. Duroc, Large White, Landrace, Mangalica, wild boar). Sequence differences between the genomes of wild boars and domestic breeds were identified in variant call files, and verified using the IGV software. Wild boar, mangalica and duroc specific primers to amplify the chosen InDel regions were designed using Primer3.

After preliminary tests five markers were chosen, three wild boar specific, one Mangalica specific and one Duroc specific one. Fluorescently labelled primers were used to make the valuation easier and more accurate with capillary electrophoresis instead of gel-electrophoresis. The markers were optimised individually and in multiplex conditions and tested in samples of 11 breeds.

In conclusion, a new, faster and cheaper set was developed to separate the wild boars from the hybrids and domestic breeds. Based on the preliminary testing on wild boars, duroc and mangalica breeds zero samples resulted false negative, so it is 100% accurate. In addition, it is a much more cost- and time-effective way than testing every single sample with STR sets.

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