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Management of phytopathogens by application of green nanobiotechnology: Emerging trends and challenges
Published June 2, 2015
15-22

Nanotechnology is highly interdisciplinary and important research area in modern science. The use of nanomaterials offer major advantages due to their unique size, shape and significantly improved physical, chemical, biological and antimicrobial properties. Physicochemical and antimicrobial properties of metal nanoparticles have received much a...ttention of researchers. There are different methods i.e. chemical, physical and biological for synthesis of nanoparticles. Chemical and physical methods have some limitations, and therefore, biological methods are needed to develop environment-friendly synthesis of nanoparticles. Moreover, biological method for the production of nanoparticles is simpler than chemical method as biological agents secrete large amount of enzymes, which reduce metals and can be responsible for the synthesis and capping on nanoparticles.

Biological systems for nanoparticle synthesis include plants, fungi, bacteria, yeasts, and actinomycetes. Many plant species including Opuntia ficus-indica, Azardirachta indica, Lawsonia inermis, Triticum aestivum, Hydrilla verticillata, Citrus medica, Catharanthus roseus, Avena sativa, etc., bacteria, such as Bacillus subtilis, Sulfate-Reducing Bacteria, Pseudomonas stutzeri, Lactobacillus sp., Klebsiella aerogenes, Torulopsis sp., and fungi, like Fusarium spp. Aspergillus spp., Verticillium spp., Saccharomyces cerevisae MKY3, Phoma spp. etc. have been exploited for the synthesis of different nanoparticles. Among all biological systems, fungi have been found to be more efficient system for synthesis of metal nanoparticles as they are easy to grow, produce more biomass and secret many enzymes. We proposed the term myconanotechnology (myco = fungi, nanotechnology = the creation and exploitation of materials in the size range of 1–100 nm). Myconanotechnology is the interface between mycology and nanotechnology, and is an exciting new applied interdisciplinary science that may have considerable potential, partly due to the wide range and diversity of fungi.

Nanotechnology is the promising tool to improve agricultural productivity though delivery of genes and drug molecules to target sites at cellular levels, genetic improvement, and nano-array based gene-technologies for gene expressions in plants and also use of nanoparticles-based gene transfer for breeding of varieties resistant to different pathogens and pests. The nanoparticles like copper (Cu), silver (Ag), titanium (Ti) and chitosan have shown their potential as novel antimicrobials for the management of pathogenic microorganisms affecting agricultural crops. Different experiments confirmed that fungal hyphae and conidial germination of pathogenic fungi are significantly inhibited by copper nanoparticles. The nanotechnologies can be used for the disease detection and also for its management. The progress in development of nano-herbicides, nano-fungicides and nano-pesticides will open up new avenues in the field of management of plant pathogens. The use of different nanoparticles in agriculture will increase productivity of crop. It is the necessity of time to use nanotechnology in agriculture with extensive experimental trials. However, there are challenges particularly the toxicity, which is not a big issue as compared to fungicides and pesticides.

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Stand evaluation, crop estimation and yield analysis of winter wheat for the optimization of yields
Published May 23, 2019
103-109

The authors have been carrying out stand evaluation, crop estimation and yield analysis in winter wheat since 2012. The sampling areas were assigned at the fields of the Training Farm of the Faculty of Agricultural and Food Sciences of Széchenyi István University Mosonmagyaróvár according to the structure of the cropping system. According t...o their observations the value of field emergence is always lower than the laboratory germination. The weak emergence is important because the lower plant density cannot be compensated by the increased tillering in spite of having larger plant growth space. It is proven by the fact that they detected strong productive tillering even at 5 and 10 mm plant spacing while there were single-spiked plants at 40-50 mm plant spacing as well. The analysis revealed that the total ear mass and grain mass of wheat plants bearing two or more ears is almost the double than that of the single-spiked plants. It was a further basic experience that the largest ear of ”multiple-spiked” plants is always heavier than the single ear of one-spiked plants. Plants with intense tillering and more ears demonstrate the importance of proper seedbed preparation and drilling and the significance of sowing good quality seeds. These are the factors that determine field germination and emergence, influence the speed and intensity of initial development and by all these factors the sufficient productive tillering. The authors emphasize the use of exact and objective methods at crop estimation, e.g. the relationship between the ear mass and the yield which is in strong correlation whilst ear length and grain mass are not suitable for a precise estimation. The authors conclude that crop estimation and yield analysis must be inevitable tools of modern crop production and will be particularly important in precision agriculture. These tools also qualify the job done by farmers and helps to identify the areas that require special attention.

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