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Effect of the media on morphology of Cryphonectria parasitica (Murr.) Barr isolates and their Vegetative Compatibility Groups
38-42Views:176The most dangerous pathogen for the European and American chestnuts is the blight fungus Cryphonectria parasitica (Murrill) Barr. Short after its introduction a big number of chestnut trees were destroyed on the infested area. The control could be really complicated, because of the numerous vegetative compatibility groups of the fungus. There is a type that carries a mycovirus viz. hypovirus in the cytoplasm. We are able to control effectively this pathogen by using mycovirus-carrying strains (called hypovirulent fungal strains also). In laboratory it is easy to multiply the virulent and the hypovirulent strains of the fungus but do not easy to differentiate colonies visually on simple PDA medium. During our research, we tested different types of media, based on potato and chestnut bark extract respectively. It was observed that on potato medium the virulent strains produce more orange pigments. So it is more easy to differentiate virulent or hypovirulent isolates of chestnut blight fungus based on colony colour and morphology.
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Isolation and identification of endophytic fungi connected to Grapevine Diseases, from the Tokaj wine region, Hungary
61-66Views:224Grapevine Trunk Diseases (GTD) is one of the most important diseases in vineyards worldwide, which can be found in Hungarian vineyards as well. In Hungarian wine regions there is very little information about the occurrence of pathogens which cause GTD, in case of Tokaj wine region there is no knowledge about that, what kind of pathogens can be found in the vineyards.
The objective of our research is to assess the situation and occurrence of GTD in Tokaj wine region in cooperation with local specialists, as well as identification of pathogens which were isolated from the diseased trunks by morphological and genetic basis.
We were able to isolate endophytic fungi from all sampled grape trunk. The majority of them were determined as Diplodia seriata not only based on colony morphology, but also determined by rDNA sequences.