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Studies on the Suitability of Different Mould Media Compositions for the Mycological Evaluation of Hay Samples
34-38Views:85t evaluating mould contamination of hay samples in more than the half of the cases Mucor spp. (and Trichoderma spp. in a smaller extent) overgrew slower developing moulds, spoiling the assessment of total mould CFU and the detection of fastidious organisms, among others the toxinogenic Fusaria. In parallel microbiological evaluation of hay samples comparing to the ISO 7954 medium as reference a) the overgrowth inhibiting effect of Rose Bengal (ISO 7954-RB); b) the combined inhibiting effect of Rose Bengal and Dichloran (dichloro-mononitroaniline) in DRBC; c) the inhibition of Dichlorane in ISO 7954 (ISO 7954-DC); d) the lowering of nutrient levels to 1/4 (1/4 N ISO 7954, 1/4 N-DRBC and 1/4 N-DC); e) the inhibiting effect of dinitro – salicylic – acid (DSA), a reducing sugar binding compound, as a potential growth inhibitor (RBC-DSA) were studied. The results showed, that 1) MSZ-ISO 7954 medium codified by the official method was unsuitable for the detemination of mould count and for the detection of toxinogic spp. in hay samples. In half of the cases the overgrowth of Mucor has spoiled CFU enumeration and recognition of toxinogenic moulds; 2) Inhibitor supplemented DRBC medium (King et al., 1979) enabled early CFU enumeration by uniforming colony sizes and by efficient suppression of Mucor, but the pink background colour of the medium was disturbing the observation of tints of conidia, which were characteristical to toxinogenic moulds like Fusaria. The hypha-staining property of Rose Bengal did not prove very important; 3) According to the recent stage of our studies, the ISO 7954-Dichloran combination can be recommended for the mycological evaluation of hays and dried roughages. CFU can be enumerated early, Mucor suppressed in the same extent as with DRBC and colours are easily observable; 4) 2-4-Dinitrosalicylic-acid (DSA) proved unsuitable as inhibitor, for its poor efficiency and for its intense yellow background coulour.
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Biostimulant induce growth, chlorophyll content and fresh herbage yield of alfalfa (Medicago sativa L.) and variegated alfalfa (Medicago × varia Martyn) plant
19-25Views:109The use of biostimulants is associated with promoting plant growth by stimulating cell division and improving nutrient availability and uptake. A study was conducted at the University of Debrecen, Hungary, to examine the effect of biostimulants on alfalfa growth, chlorophyll content and fresh herbage yield. The experiment was arranged in a randomised complete block design with three biostimulant treatments plus control replicated three times. Data collected were subjected to analyses of variance using Genstat, where significantly different means were separated at a probability of 5% using the least significant difference. The findings show no different variation in plant height or chlorophyll content (SPAD) throughout the early stages of growth. Nonetheless, a notable impact was noted in the latter stages (28 days after biostimulant treatment application) on the growth of the alfalfa plant. Biostimulant treatments did not had effect on fresh yield for second through fourth cuts, but the fifth cut showed a significant effect, with T1 treatment recording the highest herbage yield of 19745 kg ha-1 followed by T2 (Tricho Immun plus Ino Green) and T3 (Tricho Immun), with yields of 19528 kg ha-1 and 17273 kg ha-1, respectively, while the T0 (control) recorded the lowest herbage yield of 12060 kg ha-1. However, the average mean yield indicated the application of biostimulants significantly increased fresh yield herbage by 20.5%. Correlation coefficient values suggested plant height at both 14 and 28 DAH (days after harvest) strongly correlated with fresh herbage yield (r = 0.7756 and 0.7455) which reflected in the increase in fresh herbage yield. Therefore, our results suggest that the use of biostimulants in alfalfa cultivation holds promise for improving growth and yield potential through their positive effects on chlorophyll content and the growth of alfalfa plant.