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  • Population genetic results of Hungarian Botrytis cinerea isolates establishing new technologies with decreased chemical usage against grey mould
    259-261
    Views:
    103

    Botrytis cinerea causes gray mold on a high number of crop plants. Information about the populations of plant pathogen fungi may help to develop new strategies for the effective and economic crop protection with reduced fungicide usage. Hungarian B. cinerea populations were characterized with using different molecular genetic parameters. B. cinerea group I strains, characterized with high rate of fenhexamid resistance, could be detected only in restricted number. The Hungarian B. cinerea populations were characterized with high genetic diversity, and the regular occurrence of sexual reproduction. These results highlight the importance of rotating different type of fungicide in the plant protection technology against grey mould.

  • Study of alternative oxidase as possible molecular marker for phylogenetic analysis of the Botrytis cinerea
    127-132
    Views:
    115

    Botrytis cinerea (teleomorph Botryotinia fuckeliana (de Bary) Whetzel) is able to attack several economically important plants causing gray rot. Botrytis cinerea species complex includes two cryptic species (B. cinerea and B. pseudocinerea) that tolerate fungicides differently. On the basis of classical taxonomic markers, the two related species are very difficult to be distinguished; therefore, their separation is usually performed using molecular methods based on the time-consuming molecular analysis of several markers. Our goal was to find markers, which are suitable for the differentiation. Testing the nucleotide sequences of the alternative oxidase encoding gene, B. cinerea and B. pseudocinerea strains were clearly differentiated. Moreover, the analysis of the protein sequences of the enzyme with the maximum likelihood method reflected well the taxonomic relationships of the different fungi.

  • Pathogenicity differences between group I and group II of Botrytis cinerea
    81-85
    Views:
    95

    Botrytis cinerea has been reported as a species complex containing two cryptic species, groups I (Botrytis pseudocinerea) and II (B. cinerea sensu stricto). In order to compare the pathogenicity of group I and group II of B. cinerea, we have selected 4 strains of group I and 4 strains of group II. The results demonstrated that competitive infection of group II was more on grape, cucumber and paprika leaves, than group I. However the results on bean leaves did not correlate the applied B. cinerea group.