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The applicability of 10 ml cryotubes for sperm cryopreservation in a Hungarian carp landrace (Cyprinus carpio carpio morpha accuminatus)
93-97Views:245In our study, the comparison of 5 ml straw and 10 ml cryotube during sperm cryopreservation in a Hungarian carp landrace (Cyprinus carpio carpio morpha accuminatus) was carried out. Three different dilution ratios (1:1, 1:4 and 1:9) were also tested using the cryotube. A significantly higher pMOT was recorded using the cryotube in comparison with the straw. VCL and STR were similar in both groups. Cryopreservation had a negative effect on pMOT and VCl using the cryotube and also the straw where, STR was not reduced significantly. An increasing tendency was observed using higher dilution of sperm during cryopreservation; however, significant difference was not recorded between the three groups. VCL and STR were similar in all groups. Cryotube was applicable for the sperm cryopreservation of the Hungarian carp landrace. However, the standardization for the freezing method specified for this volume is recommended. The different dilution ratios needed be tested also during fertilization.
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Optimizing fish structure in angling ponds focusing on white fish
33-36Views:184According to new amendments of laws, commercial fishing licenses of natural waters shall not be issued from the calendar year 2016 in Hungary. However, the most settlement of white fish to angler ponds originated from natural waters. Currently, there is no sufficient quantity of fish species belonging to this group available to fulfil the settlement commitments of fish farmers. The aim of this recent study is to develop a breeding and production technology that is suitable for settlement of large quantities of this fish species of appropriate length of (4–5 cm). The following methods are investigated: aquaculture cage system (pond-in-pond system), monoculture and mixed population with different ratio of white fish in fish structure. Due to the different environmental needs of different fish species, many parameters need to be investigated, for example: stocking density, feeding, oxygen level, monoculture or mixed populations, growth potential and mortality. A preliminary experiment was carried out following the artificial propagation of ide (Leuciscus idus) following a 21-day larval rearing experiment in which the effect of different stoking density was investigated for mortality and growth. On the basis of the expected results, the optimal conditions for the aquaculture cage system will be described, regarding to the white fish stock.
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The comparison of sperm motility and density in four different goldfish (Carassius auratus) types
135-140Views:192Different goldfish types play an important role both in ornamental fish farming and science. Considering its historical background, the goldfish is a suitable model animal for the study of artificial selection as well as for developmental biological studies. Sperm motility and cell density is an important parameter in determining sperm quality. The aim of our study was to examine the effects of different goldfish types on the sperm quality. Several sperm motility parameters (progressive motility (pMOT, %), straight line velocity (VSL, μm s-1), curvilinear velocity (VCL, μm s-1), linearity (LIN, %), amplitude lateral Head Displacement (ALH, μm), Beat Cross Frequency (BCF, Hz)) of four different goldfish types (Common goldfish-“wild type” N=5, Shubunkin N=4, Black Moor N=4, Oranda N=5) was compared during 60 hours (at 12-hour intervals) at refrigerated storage (4 °C). The variability of sperm density was also investigated in all types. A similar cell concentration was determined in the four goldfish variants (Common goldfish 2.01*1010 ± 3.46*109; Shubunkin 1.71*1010 ± 3.25*109; Black Moor 1.66*1010 ± 3.02*109; Oranda 1.56*1010 ± 5.83*109). Statistically significant difference between the 4 goldfish types in the motility parameters and cell density was not noted. However, a decreasing tendency in Black Moor sperm motility parameters (pMOT, VCL and VSL) was observable, as well as a reduced spermatozoa density in Oranda was also recorded. Our results can contribute to the improvement of the common hatchery propagation of goldfish. Future studies can add more evidence of the possible effects of artificial selection on the reproduction in different goldfish types.