Search
Search Results
-
Comparative analysis of Staphylococcus aureus strains by molecular microbiology methods
34-39Views:167Staphylococcus aureus is a very important pathogen for dairy farms and milk processing plants. Subclinical mastitis is often caused by this species, and it can contaminate bulk tank milk when milking cows are suffering from mastitis. Additionally, thermostable enterotoxins (SE) produced by some types of this bacterium can cause food poisoning.
The aim of our research was to examine the number of S. aureus in bulk tank milk in two dairy farms and the enterotoxin-producing ability, genetic relation (pulsotype) and antibiotic resistance of S. aureus strains from different sources (bulk tank milk, udder quarter milk and environment).
The results show that the mean number of S. aureus of bulk tank milk of two farms significantly differed (P<0.05). Fourteen isolates were selected for further molecular genetic studies (five isolates were from bulk tank milk and nine isolates were from udder quarter milk). S. aureus was not recovered from the environmental samples. Three of the fourteen isolates (21.4%) tested by multiplex PCR were positive for SE genes. Two isolates carried one gene (seb) and one isolate carried two genes (seg and sei). The fourteen strains were classified into three pulsotypes and two subtypes at 86% similarity level. Isolates from bulk tank milk (n=5), were divided into 2 pulsotypes (A, C) and one subtype (C1). The isolates from udder quarter milk (n=9) belonged to three different pulsotypes (A, B, C) and two subtypes (A1, C1). The distribution of pulsotypes in the present study revealed genetic relationship between S. aureus isolated from udder quarter milk and bulk tank milk. This could be explained by the fact that in farms with a high number of infected cows, these cows could represent the main source of contamination. The results of the antibiotic resistance investigations show, that all strains were susceptible to methicillin, cefoxitin, lincomycin, tetracycline, erythromycin and sulfamethoxazole/trimethoprim. Thirteen out of fourteen strains were resistant to penicillin (A and C pulsotypes, A1 and C1 subtypes) and just one isolate was susceptible (B pulsotype) to all antibiotics tested. -
Application of hydrological models for agri-environmental management based on international and national experiences
45-50Views:172The ecological restoration of surface water flows requires the use of complex GIS and hydrological models. The HEC -RAS and MIKE11 programs calculate the hydraulic characteristics of runoff using the geometry and bed resistance the of flows. The presentation of some possible applications made through an example of the Berettyó, which is an extremely regulated river. The river belongs to the meandering category by Parker's classification system, which uses the relationship of the slope-Froude number and the depth- width ratio. Three coefficient of the four determining factor (entrenchment ratio, slope of water level, width-depth ratio) also indicates a mediocre to high sinuosity, while the river regulations established nearly straight bed-line. The standard water flow rates and characteristics have been determined based on the modeled Froude and Reynolds numbers. The flow conditions shifted to the laminar type by the changed line drawing which is ecologically lower status. Based on the results the morphological and ecohydrological river rehabilitation is appropriate.
-
The Effect of Soil Coverings on Cellulose Decomposition Activity of Sandy Soil
15-20Views:82The purpose of our experiments is to discover the effect of different soil cover matter (agroszövet and black polyethylene) on actual (under field circumstances) and potential (under laboratory circumstances) cellulose decomposition activity. In our field research, the Unger-test was used, and in laboratory research, the Petkov-Markova method was applied. In the first year of the experiment (2000) actual cellulose decomposition activity was significantly higher in covered than in the uncovered soil both in spring and autumn. The difference between the two treatments was significant only in spring. In the spring of 2001 black polyethylene showed significantly the lowest, activity, while in autumn the agroszövet (a porous black polyethylene) showed significantly the highest activity. In the autumn of 2001 the soil covered by black polyethylene gave non-significant,and the soil covered by agroszövet gave a significant higher activity value than the control. Averaging the two experimental years (2000-2001), the actual cellulose decomposition activity was significantly higher in covered soils both in spring (with 30-39%) and in autumn (with 34-69%). Moreover, in autumn a significantly higher value was detected under agroszövet than in any other treatment. The difference between the effect of treatments was not significant. In 2000, the potential cellulose decomposition activity was the highest in soil covered by agroszövet in spring, but in autumn higher activity value was detected in every covered soil than in the control. In the spring of 2001, every covered soil showed a lower, but in autumn a higher, potential cellulose decomposition rate than the control. The activity decreased significantly 27 (agroszövet) and 45 (black polyethylene) percent in spring, and increased no- significantly 8 (agroszövet) and 4 (black polyethylene) percent in autumn. During the two experimental years, we observed on average lower potential cellulose decomposition activity (15-60%) in spring and a higher one (14-43%) in autumn. Neither was significant. The dynamic of potential cellulose decomposition activity averaging 2000 and 2001 showed a slight, the actual cellulose decomposition activity an explicit non-significant upward tendency. There was a strong (r=0,189) correlation obtained between the actual and potential cellulose decomposition activity of soil, and a medium-strong (r=0,673) relationship between the soil moisture content and actual cellulose decomposition activity.
-
The influence of fertilization and tillage method on the formation root system capacity and grain production of spring barley
89-93Views:134To evaluate the interaction of year x variety, year x tillage method and year x fertilization on the grain yield and root system capacity (RSC) of spring barley, we ran polyfactorial field trials in agroecological conditions of a warm corn production area in Slovakia, at Malanta, in 2009 and 2010. The RSC measurements were done using LCR - meter at a frequency of 1 kHz and they took place in four growth stages: at leaf development in the stage of four leaves (RSC1), in full tillering (RSC2), in the stage heading (RSC 3) and at the stage of ripening (RSC4). The values of grain yield, RSC1, RSC2, RSC3, RSC4 reached in 2009 comparison to 2010 were significantly lower. The highest yield in 2009 was reached by variety Marthe (4.49 t.ha-1) and by variety Bojos (7.19 t ha-1) in 2010. The highest values of RSC in observed growth stages were achieved by variety Bojos in 2009, and in 2010 also besides RSC1. Within both years, difference in yields between tillage methods was not observed. The values of RSC in growth stage of 4 leaves and tillering was higher at conventional tillage, butthe values of RSC3 and RSC4 were higher with minimized tillage. The highest grain yield and values of RSC in every growth stage were achieved on the fertilization variant “c“ in 2009 and on the fertilization variant “b“ in 2010. The correlation relationships between grain yield and RSC were significant and positive in every growth stage. The strongest relationship was found among grain yield and RSC (r=0.6047).
-
Study of plant physiological parameters in winter oilseed rape (Brassica napus var. napus f. biennis L.) production on chernozem soil
111-115Views:259We made plant physiology examinations in Arkaso winter oilseed rape hybrid substance: relative chlorophyll content (SPAD) and leaf area index (LAI) measurements. The experiment was set in University of Debrecen Agricultural Sciences Center at Látóképi Experimental Station in four replications, in two different sowing times (I. sowing date on 08/22/2014 and II. sowing date on 09/09/2014 sowing againhappened because of the incomplete germination in the second subtance 01/10/2014) Three different plant density 200, 350 and 500 thousand ha-1, under the same nutrient supply, 45 cm row spacing. The experiment was green crop of winter wheat. The relative chlorophyll content (SPAD) and leaf area index (LAI) measurements were made in seven different times. We measured the maximum value of chlorophyll content in the first sowing time at 500, and the second sowing time at 350 thousand ha-1 plant density. The measurement results proved that there was a linear relationship between the number of plants and the LAI. The maximum leaf area index values we measured in both the sowing time at 500 thousand ha-1 reached.