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Micropropagation of Rudbeckia hirta L. from seedling explants
53-59Views:119We conducted experiments for developing an in vitro micropropagation protocol starting from meristems of Rudbeckia hirta L seedlings. We pre-soaked the seeds in sterile ion-exchanged water for 17 hours, and then achieved surface disinfection in two separate steps. First, we used concentrated household sodium-hypochloride solution for 20 minutes and, also for 20 minutes, we applied hydrogen peroxide of 10%, which was followed by washing with sterile ion-exchanged water three times. For the propagation of seedling meristems, the combination of half-strength solid Murashige and Skoog (1962) culture medium containing 10 mg/l of kinetin and 2 mg/l of kinetin + 0.1 mg/l of 2iP proved to be the most suitable. The average number of shoot-buds developed from the seedling axillary meristem in the best culture media varied between 5 and 17. Without separating them, we inoculated the shoot-bud clusters on MS culture medium containing 2 mg/l of IAA. After four weeks of incubation, we obtained elongated shoots, which we separated and inoculated into a new culture medium and from which we obtained elongated roots. The rooted plants were gradually acclimatised in the cultivation room, potted and carried to a greenhouse, and then planted in open field for subsequent observation. By adopting this method, our laboratory started the micropropagation of the superior and/or elite genotypes of the Rudbeckia hirta L. being of special value in respectt to breeding.
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The use of renewable resources is an opportunity and an obligation
13-17Views:115The renewable energy sources could be used in energy production, while no or only very slightly emit harmful substances to the environment. The solar, wind, hydropower, biomass and heat rational utilization of land contributes to greenhouse gas emissions.
Renewable energy sources also reduces the dependence on fossil fuels, thus contributing to increase security of supply. The creation of local jobs to strengthen the area's population retaining ability. -
A new model for predicting carbon storage dynamics and emissions related to the waste management of wood products: introduction of the HWP-RIAL model
75-81Views:153The ability of wood products to store carbon allows for their significant contribution to the climate mitigation efforts and the emission reduction commitments set by the EU. In order to optimise the carbon storage capacity of wood products, it is important to take climate mitigation aspects into consideration as much as possible during their production, use and waste management. The aim of this study was to quantify the effects of product development, recycling, and waste management technologies on carbon storage and emissions. In the frame of the ForestLab project, a new model and decision support tool was developed, which is able to predict the duration of carbon storage of wood products and the evolution of emissions from them. The developed HWP-RIAL model (Harvested Wood Product Recycling, Incineration And Landfill model) uses the methodology of the Intergovernmental Panel on Climate Change (IPCC) to calculate emissions, which is also used in the National Greenhouse Gas Inventory report. It combines the IPCC waste model with equations describing the carbon storage and emissions of wood products, and the model is also supplemented with a self-developed recycling and waste routing module. This paper provides insight into the operation of the model by following the life cycle of 200,000 m3 particle board.