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Identification of the Slovak traditional cheese “Parenica” microflora
227-239Views:458Numerous studies have demonstrated the higher accuracy, faster time-to-results and lower costs provided by MALDI Biotyper systems compared to classical methods. In this study, the culturable population of total count of bacteria, enterococci, coliforms bacteria, lactic acid bacteria (LAB) and microscopic fungi and yeasts from cow’s dairy products was identified using the MALDI-TOF MS Biotyper. Altogether, 50 samples of the Slovak cheese “Parenica” were examined. Total numbers of bacteria were cultured on Plate count agar at 37 °C for 24–48 h, aerobically; enterococci were cultured on Enterococcus selective agar at 37 °C for 24–48 h, aerobically; coliforms bacteria were cultured on Violet Red Bile lactose agar at 37 °C for 24–48 h, aerobically. The LAB were cultured on MRS (Main Rogosa agar), MSE and APT agar at 30 °C in microaerophilic conditions. The microscopic fungi and yeasts were cultured on Malt extract agar at 25 °C for 5 days, aerobically. Isolated strains (total 669) were subjected to identification by the MALDI-TOF MS. Among total count the identified bacteria mostly were Acinetobacter baumannii, Bacillus cereus, Micrococcus luteus and Staphylococcus warneri. Escherichia coli and Enterobacter cloacae were the most abundant coliform bacteria representatives identified. Coliform bacteria included Citrobacter, Hafnia and Klebsiella. Altogether three genera belonged to the LAB – Lactobacillus, Lactococcus and Leuconostoc were identified with Lactococcus lactis, Lactobacillus plantarum, Lactobacillus coryniformis, L. fructivorans and Leuconostoc mesenteroides were considered as the dominated LAB species in dairy products. Among yeasts, Kluyveromyces lactis, Candida zeylanoides and Yarrowia lipolytica were among the most isolated.
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Correlation of pruning time and fruiting part differentiation of sweet cherry cultivars
131-134Views:248There are several extended studies in sweet cherry production in Hungary and all over the world i.e. for creation and maintainence of smaller tree crown and high density orchards. The use of suitable dwarf rootstocks for this fruit species are very limited. On one hand, most of the draf rootstocks do not cause enough growth reduction and on the other hand these rootstocks are get old very quikcly and their fruits become small, and therefore, they not serve the requirements for intensification. In summary, there is a need for those rootstock which are vital, regeneration enhanching and delay ageing. Due to ensuring above features, Prunus mahaleb is still an obvious solution for intesive production. Increasing intensification can be obtained by use of modernisation of technological elements and suitable cultivar choice.
According to this increasement of intensity through application of novel technological elements (timing, manner and severity of pruning) and selection of the proper cultivar is implement able. Important differences are experienced between sweet cherry cultivars in their growth attributes, light demand and dynamics of fall back in regenerative potencial of different aged wood parts. From this point knowledge of the abow detailed is very important in order to maintain rentability of already established plantations. Our work shows the formation of production part in
9-year old plantation with spike spindle and free spindle crown forms depending on pruning timing (winter, summer) and determining of their various effects. -
The effects of education, culture, research and development in Hajdú-Bihar county
87-90Views:645The most important instrument of social advancement and economic development is the education system. The educational qualification of the population of Hajdú-Bihar county is continuously improving, however the census data of the year 2001 show that the educational qualification of the population of the county did not reach the national average. The educational qualification of the county’s population is higher at the county seat and in other cities and lower in small municipalities. The county’s ratio is strongly influenced by the data of the county seat Debrecen and that of Hajdúszoboszló sub region.
The unfavorable educational level of the region is related to the migration tendencies and the brain-draining trends of the past decades, as a consequence of which the population of the less developed eastern regions has moved either to the capital, or to the county seats, or larger cities of the region. The young are increasingly affected by the process.
The number of kindergartens in the county corresponds to the national average, therefore taking into consideration the regional demographic characteristics the aim should be the equalization of regional disproportions. The number of students per 1000 resident within the Northern Great Plain Region and in the county significantly exceeds the national average. The secondary school network of the county consists of 38 secondary grammar- and 51 secondary vocational schools.
The secondary schools of Debrecen have considerable impact on the county’s secondary education. The vocational training network of Hajdú-Bihar county is a large, in its conditions highly differentiated institutional network in which all types of secondary vocational institutions are present with secondary technical-, vocational-, accredited higher vocational-, specialized or preparatory vocational-, and special skill enhancement programmes. The majority of the secondary institutions have regional, rarely national enrollment.
Students who have the intention to continue their studies in higher education within the county can study in Debrecen or Hajdúböszörmény.
The integrated University of Debrecen has the highest student number within the country and it offers the widest range of academic programmes in Hungarian higher education. The University of Debrecen with the wide range of graduate and postgraduate educational programmes, unified academic qualification system, English language programmes is a European standard scientific centre of the Trans-Tisza Region. The county’s higher education takes exceeding share beyond its weight in the education of Natural Sciences (Biology, Physics, Chemistry, Earth Sciences, Mathematics), Health- and Social Sciences, Agricultural- and Environmental Sciences and Information Technology, which positively influences its educational structure. The county’s share in the education of Humanities (Hungarian Language, Foreign Languages, History, Archeology, Philosophy) is also significant.
6.3% of the country’s scientific researchers work in the county. In 2010 7.0% of the country’s R+D expenditure was spent here. 3/4 of the region’s research capacity is in Hajdú-Bihar county employed either by the University of Debrecen or by the research teams of the Hungarian Academy of Sciences.
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Coagulase-negative staphylococci in ewe udder surface and raw milk samples
221-225Views:553Coagulase-negative staphylococci (CNS) are among the major causes of subclinical mastitis in dairy ewe flocks. This has a financially significant impact on the ewe dairy sector and consumer health. The determination of the bacterial count, particularly CNS, is critical in terms of the quantity and quality of ovine milk. Thus, the purpose of this study was to quantify the CNS count in the udder surface and raw milk samples of the ewe, in addition to identifying CNS strains (n=8) collected from udder surface and individual raw milk samples by 16S rRNA gene sequencing. A total of 164 samples of udder surface and raw milk originated from four sheep farms were tested. The obtained values were compared between the different farms. Furthermore, values during 2018 and 2019 in the case of Farm I were compared. Significantly higher (p<0.05) average count was observed in udder surface samples taken from Farm I (2.8±1.0 lg CFU/cm2) than that of Farm III samples (2.3±0.6 lg CFU/cm2). In the case of individual raw milk, the higher value was observed in samples derived from Farm III (3.5±0.9 lg CFU/mL), while the obtained value was significantly lower (p<0.05) in samples originated from Farm IV (1.8±0.4 lg CFU/mL). In the bulk tank milk samples, the highest mean value was 5.3±0.4 CFU/mL, and there was no significant (p>0.05) variation between farms. Coagulase-negative staphylococci counts were decreased to a certain extent in both sample types tested during 2019 except for individual raw milk derived from the Tsigai breed. The correlation between the mean CNS counts of udder surface and individual raw milk was very weak (r=0.048). Staphylococcus simulans, Staphylococcus auricularis, and Staphylococcus equorum were identified by molecular sequencing and Staphylococcus simulans were the most frequently identified CNS species. A higher CNS count of bulk tank milk than individual raw milk indicates possible contamination during milking and storage. Therefore, further studies are required to investigate the other sources of bulk tank milk contamination to improve the hygienic quality of milk.
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Evaluation of KCl-EDTA-, CaCl2- DTPA-, CaCl2-DTPA-TEA extractants for estimating available Cu in soils
5-9Views:414KCl-EDTA-, CaCl2-DTPA-, CaCl2-DTPA-TEA extraction
methods were compared to determine the plant available Cu
amounts in the soil samples. In the soil extracts, the amounts of
copper were measurable. In the buffered CaCl2-DTPA-TEA
extracts, data showed the lowest values.
On the applied soils, a correlation was observed between
plant removal and soil Cu using extractants mentioned above. The
closest correlation appeared in the case of sandy soils.
From the results of statistical analysis, it appears that soil
properties may play role in the efficiency of the extraction using
either CaCl2-DTPA or KCl-EDTA solutions.
To establish more reliable relations, further studies with
different types of soils are needed. -
Comparison of macroelement and microelement contents of conventionally and organically grown crops
87-92Views:596Nowedays one of the most important issues to discuss is the healthy nutrition: feeding our bodies with high quality nutrients, which is free of chemical residues. The demand for healthy and nutritious food is increasing worldwide, that results in the growing popularity of consumption of organic food. Several studies dealing with the nutrient content of traditional and organic food have been published lately, since it became clear that eating food contaminated with chemical residues damage to health, food and the environment. Therefore it would be desirable to find out if organic food is really superior to conventional one. In our study we analyzed the dry matter, ash, macroelement and trace element content of organically and conventionally grown crops (carrot root, potato tuber and parsnip). Our results provide further information to consumers about the nutritional value of organic and conventional crops.
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Soil moisture sensors for sustainable water management in field crop production: A review of advances and application challenges
41-54Views:565Efficient water management is essential for sustainable production of field crops amid climate change, population growth, and water scarcity. Traditional irrigation practices often lead to water use inefficiency, which harms soil health and reduces yields. To address this, reviewing previous studies on soil moisture sensors provides important context and guidance. Literature from Scopus, Google Scholar, and WoS (2019–2025) on soil moisture sensors for sustainable water management in field crops was screened. Out of 244 retrieved publications, 79 met the inclusion criteria with a focus on sensor technologies, applications, advances, and challenges, analysed thematically for research gaps and insights. Based on the findings, soil moisture sensors boost water management, improve yields of field crops, and support sustainable agriculture. However, hindrances related to high costs, lack of awareness, technical complexity, calibration needs, energy challenges, data interpretation difficulties, and compatibility problems hinder effective soil moisture sensor results. Integrating soil moisture sensors with decision-support tools optimises water use and protects soil health to promote long-term productivity under climate variability. Future research should strategise on the development of low-cost, reliable soil moisture sensors with technology subsidies, training, policy support, durability, integration, and simple data to empower farmers to adopt precision water management.
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Phytoplasma infection status survey in plum psyllid (Cacopsylla pruni) population
45-48Views:363European Stone Fruit Yellows (ESFY) phytoplasma disease causes an increasing amount of damage. This is especially true to the Gönci growing region. The insect vector of the disease, which has been shown to have a vector role during transmission experiments, is the plum psyllid (Cacopsylla pruni). In 2018, during the swarming period of plum psyllid, in 3 different settlements (Boldogkőváralja, Nagyvárad and Bekecs) 265 psyllidswere collected, from which 165 were plum psyllid. Molecular biology studies showed 106 individuals infected with phytoplasma, of which 20 were males and 86 were females.
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Effect of floor type and bedding system on sows lifespan
155-160Views:316The paper studies two nucleus pig farms in the Great Plain region of Hungary which have same management, same feeding system but different breeding technology (Farm A had solid floor with straw and Farm B had slatted floor without straw). The genetics of sows were crossbred Dutch Large White and Dutch Landrace. The comparative examination was based on the causes of culling according to the different floor type. To the analysis of culling reasons was applied the methods of survival analysis.
In the analysis there were used the Kaplan-Meier method and the Cox Proportional Hazard model to the estimate the difference between the farms. The results of survival analysis showed significant difference (p<0.05) between the lifespan of sows that caused the difference between the survival probabilities of sows culled due to lameness. Furthermore there was showed that the sows kept on solid floor with straw have stayed in production longer than on slatted floor without straw. The hazard ratio was 1.434 that means that the culling due to lameness is 1.434 times higher by the slatted floor without straw facing by solid floor with straw.
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Genetic discontinuity analysis of Collared Dove (Streptopelia decaocto)
5-11Views:506The Collared Dove conquered continent areas within a few decades. Causes and dispersion pattern of expansion has been investigated in several studies. However, the relationship between the geographic distribution and genetic structure of populations has not been researched. We used 152 individuals from 19 countries in this study. We analyze a 650 bp long mitochondrial COI sequences of each individuals. We were performed Spatial Autocorrelation Analysis, Principal Component Analysis and analysis of the genetic discontinuity in this study. Under 2500 km distance was a positive correlation between the genetic differentiation and different geographical areas. Hidden genetic barriers were found only Carpatian Basin. Could not be detected signs of genetic isolation in other regions. This will probably due to the unevenness of the sample collection, because these areas proportionally much fewer sequences were available. Therefore, is worth repeat this analysis after further sample collection, in the future.
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The roles of mycotoxins in cereal crops production: A comparative study of Hungary and Tanzania
151-159Views:805Although Hungary and Tanzania's climatic, soil, and technological conditions differ significantly in crop production, cereals crop cultivation is of paramount importance; maize crop dominates the cultivated area (Hungary 1 million ha, Tanzania 3 million ha) both from a feed and food point of view. Unfortunately, in both countries, fungal species (Fusaria, Aspergilli, Penicillia, etc.) that produce various mycotoxins on cereals, including maize grains, are a growing concern. The situation is complicated because these fungal species and their toxins can appear not only on cereals but also on other crops. Despite the prevalence of mycotoxins in both countries, studies show higher exposure risks and contamination above tolerable levels for human consumption in Tanzania to Hungary, with Tanzania observing acute aflatoxicosis.
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The effect of sunburn damage on the fruit quality parameters of apple (Malus domestica cv. Idared)
55-64Views:2309In the present study the authors investigated the effect of sunburn injury on fruit quality parameters (cover colour, depth of tissue damage, fruit flesh firmness, dry matter content) of apple.
The symptoms of sunburn injury appeared as concentric rings, differing in colour from each other and the cover colour. This can be connected with the ratio of the injury. The authors observed the following colours on the fruit surface (from the epicentre of spots on the surface of the fruit) dark brown (strongly damaged), light brown (moderately damaged), pale red transition (weakly damaged), red surface cover colour (not damaged).
Sunburn of apple fruits is a surface injury caused by solar radiation, heat and low relative humidity. In the initial phase, a light corky layer, golden or bronze discolouration and injuries of the epidermal tissue appear on the surface exposed to radiation. Thus, it detracts from the fruit’s appearance, but in most of the cases it would not cause serious damages in the epidermal tissue. The depth of tissue damage is not considerable, its values are between 1.5-2.0 mm in general. It is commonly known, that tissue structure of the apple fruit is not homogeneous. Accordingly, the degree of injury shows some differences under the different parts of the fruit surface.
On the basis of the flesh firmness studies, it can be stated that the flesh firmness of the damaged parts increases due to the sunburn effect. This is due to the fact that the damaged plant cells die, the water content of the tissue decreases and it hardens. However, due to this reduction in the water content the dry matter content will increase. -
Seed biology and possibility of improvement of seed germination capacity on Virginia mallow (Sida hermaphrodita L. Rusby)
71-76Views:307Sida hermephrodita or Virginia mallow is a perspective perennial herb in the Malvaceae family able to yield a biomass crop through the last two decades. Additionally, the plants have a lot of uses and benefits for instance it can use as a fodder crop, honey crop, ornamental plant in public gardens. It has favourable features like fast growing and resistance against the disease and climatic fluctuations, etc. Sida is in the beginning phase of domestication therefore it has a serious disadvantage: the low and slow germination as a big part of wild plants. Due to the expressly low germination percent the need of seed showing of driller is should tenfold, 200 thousand seeds/acre instead of 10–20 thousand what is not available and expensive Therefore practical purposes of our research of seed physiology was to increase the seed germination percent in a available, basically wild Sida population. In the first stage of our experiments we examined two factors relating to seed germination percent and seed germination power during our research: the influence of hot water treatment and the effect of exogenous or endogenous infection of seed. However, in our germination tests, utilizing scarified seeds with hot water (65 oC, 80 oC, 90 oC), from 29,3% to 46% germinated from those samples which were collected from the population of Sida hermaphrodita in Debrecen. The average germination for all season was 5–10% without treatment and rinsed using hot water up to almost 50%. When physically scarified used, the oldest seeds showed the best germination (46%) after the hot water operation in spite of the previous studies. We discovered that apparently there are close relationship between the seed fresh weight or water uptake capability and the percentage of infection. Following these recognition we modified our technique,in such a way that we fractionated the seeds based on their fresh weight/or relative density before we carried out the treatment. When we filtered the floating seeds on the surface of water, the hot water treatment was performed considerably better on the sunk seeds after separation. Therefore, by this special priming process we were able to reach 80% germination capacity of Virgina mallow seeds under laboratory conditions (26 oC without illumination).
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A review on the evaluation of horse coat color
19-26Views:42The coat color diversity of horses represents a classic example of the genetic and evolutionary effects of domestication. While wild equids exhibited limited pigmentation patterns, human-mediated selection has dramatically expanded phenotypic and genetic variation among domestic horses. This variation is influenced by multiple interacting loci, with some genes exerting pleiotropic effects on physiological and morphological traits. Modern molecular genetics has identified key loci controlling base colors (Agouti, Extension), dilution (D locus), greying (G locus), and patterned phenotypes including Tobiano and Overo, while also highlighting the increasing importance of additional white patterning mechanisms such as MITF and PAX3-associated Splashed White phenotypes and KIT gene complex variants (e.g., Sabino 1 and W1–W30 alleles). Furthermore, some coat color–associated mutations illustrate the fitness costs of selection, as seen in Overo Lethal White Syndrome (OLWS) and the association between the Silver dapple mutation and Multiple Congenital Ocular Anomalies (MCOA), emphasizing the pleiotropic and sometimes deleterious effects of pigmentation genes). Quantitative assessment using Lab* color metrics allows objective evaluation of coat pigmentation and facilitates comparisons across breeds, ages, and sexes. Phenotypic studies show that coat color does not directly correlate with performance or temperament but may affect thermoregulation, predator avoidance, disease susceptibility, and market value. Comparative research on other livestock species confirms that coat color is largely polygenic, heritable, and influenced by human selection. Integrating molecular and phenotypic analyses enhances our understanding of the evolutionary, genetic, and practical implications of coat color variation in horses. These insights support breeding decisions, breed identification, and the conservation of genetic diversity. Overall, the study of equine coat pigmentation provides a valuable model for investigating domestication, human-driven selection, and the genetic architecture of complex traits in livestock species.
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Phylogenetic studies of Phoma species by maximum likelihood analysis
37-46Views:438The cosmopolitan Phoma genus contains mainly phytopathogenic, opportunistic parasite, and saprophyte fungal species. Up to now the characterization of Phoma species and other taxa of Phoma has so far been determined on the basis of morphology on standardized media, and gene sequence analysis was only used as a confirmative or distinctive complement.
In this study we have tried to study phylogenetic relationships by maximum likelihood method in the Phoma genus. We employed a part of the gene responsible for the synthesis of translation elongation factor 1 subunit alpha protein (tef1) containing both introns and exons, a part of the gene responsible for synthesis of tubulin protein and ITS region containing the internal transcribed spacer regions 1 and 2 and the 5.8S rDNA as potential genetic markers to infer phylogenetic relationships among different Phoma taxa. Twenty-four isolates of eleven different Phoma species were firstly characterised by morphologically, and then their tef1, tubulin and ITS sequences were sequenced and analysed by maximum likelihood method carried out by PAUP*4.0b program. According to constructed phylogenetic trees, the different Phoma taxons are well separated. However these trees do not support the traditional Phoma sections based on morphological characterization.
The maximum likelihood analyses of all three sequences confirmed that the Phyllosticta sojicola species is clustered with the Phoma exigua var. exigua group and the Phoma sojicola is grouped with Phoma pinodella group. The experienced molecular evidences initiate the demand of reclassification of formerly mentioned soybean pathogens. -
Mapping agricultural performance and environmental parameters aimed at generic regional studies
29-34Views:563In this paper we present the interim results and the methodology applied to create web GIS ready cartographic representations of agricultural performance related information and environmental parameters. The main aim of the research is to eventually create a web GIS based decision support system that can enable decsision makers and general users to create useful and representative map layouts of certain environment and agriculture related phenomena that can be easily analyzed and interpreted to make strategic decisions on environmental issues. In this aim the initial steps are to evaluate the available data for cartographic representation, analyze the possibilities of visualization, create a GIS ready data structure and implement the database and revise additional possibilities to incorporate further environment related datasets of auxiliary sources. The main results of the study are a comprehensive set of visual layouts that could serve as guideline for mapping statistical information of agriculture and some steps towards the incorporation of environmental parameters to the system.