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• SNP analysis of ITS1-5.8S-ITS2 rDNA loci in modern and ancient melons (Cucumis melo)

  120-124

  Zoltán Szabó

  Gábor Gyulai

  Zoltán Tóth

  László Heszky

  Views:

  307

  ITS (internal tanscribed spacer) profiles of the aDNA (ancient DNA) of seed remains extracted from an extinct sample recovered from the 15th century (Budapest, Hungary) were compared to 31 modern melon cultivars and landraces. An aseptic incubation followed by ITS analysis was used to exclude the exogenously and endogenously contaminated (Aspergillus) medieval seeds and to detect SNPs in ITS1-5.8S-ITS2 region of rDNA (ribosomal DNA). SNPs were observed at the 94–95 bp (GC to either RC, RS or AG) of ITS1; and at 414 bp (A-to-T substitution), 470 bp (T to Y or C), 610 bp (A to R or G) of ITS2. The results facilitate the final aim of molecular and morphological reconstructions of ancient melon tpyes.

• Routine microscopy examination of faecal samples as a tool for detection of common gastrointestinal parasites: a preliminary report from two Hungarian farms

  63-66

  Rojesh Khangembam

  Levente Czeglédi

  Nóra Vass

  Views:

  407

  Gastrointestinal parasitism in ruminant animals is a cause of major economic loss incurred by the livestock industry. Regardless of the frequency of the adopted therapeutic and prophylactic deworming strategies, the parasitic burden in a farm should be assessed regularly. One of the most widely used techniques to do so is the microscopic faecal egg examination and faecal egg counting method. Despite the technique being almost a century old from its first adoption, the principle behind the newer techniques of faecal egg examination is the same. This technique is still being used in routine farm screening and monitoring gastrointestinal parasitic load and faecal egg count reduction testing to assess the anthelmintic efficacy of the drugs used. Thus, the tool remains a choice for preliminary screening for important parasites and the subsequent deworming strategy. Our study here was part of a larger survey on the treatment efficiency as well as a broad epidemiological study of the trichostrongyle parasites in Hungary. We present a preliminary report on the detection of common gastrointestinal parasites from two farms in Hungary, including a species-specific confirmatory microscopy for Haemonchus contortus eggs.

• Development alternatives of rural economy

  187-191

  József Káposzta

  Views:

  208

  Environmental, natural, social and economic processes undergoing both in Europe and in the world predict such a 21^st century that is characterised by increasing resource-crisis from both economic and ecological aspects. Therefore, it is very important for Hungary to see what happens to its natural resources, epecially to its agricultural land, water reserves as well as the biodiversity of the local unique flora and fauna. One of the most significant issues of the rural areas of Hungary is whether we can preserve the natural habitats and the various biodiversity of the related species, the favourable biological background of agriculture. In addition, whether we are able to provide high quality food for the country as well as for the broader reagion, whether we are able to produce energy from the resources available as well as to provide sufficient opportunities for the population to  live and work. These can be considered as the most significant issuesof the coming decades which determine the strategy of the Hungarian rural economies in long term.