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Examination of the usability of the MALDI-TOF method in sunflower genetic identity analyses
33-38Views:147Economically, one of our most important crop is the sunflower. Regarding its production it is essential to use top quality seeds. As the conditions of seed certification and the certification parameters are regulated by laws and ordinances, it is highly important to farmers and seed producers to detect seeds of low quality or dubious origins.
Nowadays, the examination of the cultivar homogenity of sunflower is based on a reference method of the International Seed Testing Association Rules International (ISTA), Chapter 8. This standard reference method uses ultrathin poliakrilamid isoelectric focusing gel electrophoresis (IEF-UTL, or simply IEF). With this work we have set out to compare the results of MALDI-TOF to the test results – used as reference results – achieved by this reference method. The aim is to develop a new, quick, cheap and reliable method. In this article I summarized the results of some of the experiments that will provide basis for my further work.
During our previous experiments we have concluded that out of four extracting agents we can get the most protein markers using NaCl acid buffer, 1 propanol buffer. -
The significance of ecolabels in tourism
118-124Views:86More than one hundred types of ecolabels have been introduced worldwide since the 1980s. These voluntary certifications may play a prominent role in achieving sustainable tourism as, on the one hand, they may encourage companies to behave responsibly, and on the other, they may contribute to increasing tourists’ awareness in their choice. Considering that there are many small and medium-sized companies working on the tourism market, developing voluntary certification systems is highly encouraged in the member states of the European Union.
At the same time, there are significant differences among the various ecolabels concerning their content, qualitative criteria, and requirements, which makes it difficult for them to gain acceptance on the tourism market. It is worth mentioning that the sets of criteria belonging to the ecolabels usually do not involve indicators related to the social and cultural impacts of tourism.
In my paper I review the main characteristics of voluntary certifications with special emphasis on the players in tourism ecolabels, furthermore I discuss their advantages and some of the arising problems as well.