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Sour cherry seed as an industrial gamma tocopherol source
27-33Views:193In our experiments sour cherry kernels were investigated. The kernels of different sour cherry cultivars were originated from Újfehértói Gyümölcstermesztési Kutató és Szaktanácsadó Nonprofit Közhasznú Kft. Tocopherol profile, oil yields and nutritional value were stood in focus of examinations.
We started processes preparing of sour cherry kernels. After the preparation recovered non-polar fraction was investigated. We worked with formerly developed chromathographic system, which is capable to separate different tocopherol isomers efficiently. We measured concentration of each tocopherol isomers and registered those and their relation in different oil samples.
According to received data, alpha-, gamma- and delta-tocopherol appeared in samples, but beta-tocopherol could not be detected. It became clear that level of gamma-tocopherol is far higher than alfa- or gamma-isomer, it is concentration exceeded 1–1.5 mg ml-1.
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Ecotoxicological impact of DON toxin on maize (Zea mays L.) germination
35-40Views:211Fusarium graminearum is one of the most significant arable pathogen in Hungary, and various types of trichothecene mycotoxins (mostly DON, deoxynivalenol) are detected most commonly in cereals (Biró et al., 2011). Fusarium infection and mycotoxin production could not be eliminated, and infected maize by Fusarium sp. cannot be exploited as food, seed, or animal feed. However it can be raw material of biogas production. In this research we would like to investigate the content and effect of the toxin in the end product of biogas production on plant germination. The Fusarium sp. can cause mildew and seedling mortality in seed of maize (Zea mays L.), so we examine the effect of this on germination. In preliminary examination Fusarium sp. was not detected in the bioreactor of the Institute after the retention time (30 day), however it can be assumed that during the hydrolysis of the fungus growth and mycotoxin production also increased exponentially. There were no appropriate tools to detect the toxin in the end product of biogas production so modelling of anaerobic hydrolysis was necessary. The effects of hydrolyzed product for germination were also detected.
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Effect of allithiamine on the level of hyperglycaemia-induced advanced glycation end products
41-44Views:292Diabetes mellitus is a rapidly growing public health burden in both developed and developing countries. Diabetes mellitus is accompanied by hyperglycaemia, which can cause tissue injury by several mechanisms. One of these is the formation of advanced glycation end-products (AGEs). In this study, the effect of allithaimine, a fat-soluble thiamine derivative, was investigated on hyperglycaemia-induced AGEs levels using human umbilical cord vein endothelial cells (HUVECs) as a hyperglycaemic model. HUVECs were isolated by enzymatic digestion, characterized by flow cytometer and treated 30 mM glucose plus allithaimine or thiamine or cell maintenance medium as control. Allithiamine was synthesized and purified. The structure of the synthesized and isolated compound was verified by reverse phase HPLC and MALDI-TOF. AGEs were evaluated by ELISA. Collectively, our results indicate that allithiamine can reduce level of the hyperglycaemia-induced AGEs similar to thiamine.
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Effect of anthocyanin-rich sour cherry extract on the level of IL-8 in LPS-induced endothelial cell
27-30Views:235The anthocyanin content of the Hungarian sour cherry is remarkable. Nutraceutical and pharmaceutical effects of the anthocyanins and their role in disease prevention have been studied extensively. Endothelial cells are involved in the pathogenesis of several inflammatory diseases. The objective of this work was to investigate pure sour cherry extract on human umbilical cord vein endothelial cells (HUVECs) as an inflammatory model. HUVECs were treated with 100 ng/mL lipopolysaccharide (LPS) and 50 mg/mL sour cherry extract or M199 medium as control. The optimal concentration range of the sour cherry extract was investigated and selected based on MTT assay measuring the conversion of the tetrazolium salt to formazan by mitochondrial dehydrogenases. The level of interleukine-8 (IL-8), a pro-inflammatory cytokine, was measured in Luminex MagPlex assay. LPS treatment significantly increased the secretion of IL-8. The pure sour cherry extract was able to attenuate this increment indicating the potent anti-inflammatory effect of pure sour cherry extract. Our results emphasize that pure sour cherry extract could reduce the LPS-induced inflammatory response thereby may improve endothelial dysfunction.