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Development of an InDel marker set to establish hybridization between wild boar and domestic pig (Sus scrofa) breeds
21-25Views:586Wild boar and domestic pig breeds belong to the same species (Sus scrofa), so they can easily have viable offspring. This could be a problem in preserving the genetic lines of wild boars, keeping clean the food industry from lower-grade hybrid boar meat, and „producing” ethically questionable trophies, too. The aim of our study was to develop a cost-efficient, fast, easy and accurate marker set which can separate the wild boars from hybrids and domestic pig breeds.
The InDel markers were developed using 59 full pig genomes of 17 different breeds (e.g. Duroc, Large White, Landrace, Mangalica, wild boar). Sequence differences between the genomes of wild boars and domestic breeds were identified in variant call files, and verified using the IGV software. Wild boar, mangalica and duroc specific primers to amplify the chosen InDel regions were designed using Primer3.
After preliminary tests five markers were chosen, three wild boar specific, one Mangalica specific and one Duroc specific one. Fluorescently labelled primers were used to make the valuation easier and more accurate with capillary electrophoresis instead of gel-electrophoresis. The markers were optimised individually and in multiplex conditions and tested in samples of 11 breeds.
In conclusion, a new, faster and cheaper set was developed to separate the wild boars from the hybrids and domestic breeds. Based on the preliminary testing on wild boars, duroc and mangalica breeds zero samples resulted false negative, so it is 100% accurate. In addition, it is a much more cost- and time-effective way than testing every single sample with STR sets.
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Effect of different n-6/n-3 fatty acid proportion oil sources on reproduction performance and fatty acid profile of milk in modern genotype sows - Pleminary results: Preliminary results
121-128Views:314This study was conducted to investigate the effects of supplemental n-6 and n-3 fatty acids on sow’s milk fatty acid composition during the lactation period and on reproductive efficiency of sows in the subsequent gestation period. Data were collected on a total of 213 DanBred sows (108 control and 105 experimental) representing parity of 2–7, respectively. Control and experimental sows were placed in the same housing conditions during lactation and gestation period. Control group received 6.3 g of sunflower oil (SO) per kg feed as n-6 fatty acid supplementation, whilst experimental animals received the same amount of fish oil (FO) as n-3 fatty acid source. Diets were corn meal-extracted soybean meal based. The experiment was conducted in one replication as being a part of a longer and more comprehensive trial. It was found that the consumed long-chain polyunsaturated fatty acids (LC-PUFAs) appeared in the sow’s milk and changed its fatty acid profile. With this alteration, the n-6/n-3 ratio of experimental (FO) sows’ milk were narrower than in SO group (SO: 13.82 vs. FO: 5.89). The benefits of n-3 fatty acids supplementation were evident for the subsequent reproduction cycle, when experimental sows heated more reliable and earlier than control (weaning to oestrus interval: SO: 5.86 vs. FO: 4.48 days). Only 2.33% of experimental sows (FO) did not heat within 7 days after weaning, but this was 12.36% in the control group (SO). The present study requires further research to evaluate the effect of n-3 fatty acids on maintenance of pregnancy and improved subsequent litter size.
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Association analysis of TNNI1/XbaI polimorphism on carcass quality in hybrid pigs
59-62Views:570The contractile protein, which is encoded by troponin I 1 (TNNI1) gene, is located on the thin filaments of slow fibres in striated muscle. TNNI1 protein is a part of the troponin complex which plays an important role in regulation of muscle contraction by preventing actin-myosin interaction in absence of calcium. According to biological role, this gene can be potential marker for meat production related traits. The aim of this study is to define whether the previously reported gene polymorphism (EU743939:g.5174T>C) is connected with the slaughter traits measured in a standard slaughterhouse of the examined four-line European hybrid. The study included data from 404 gilts and barrows from 2 different samples. The polymorphism was detected using PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) method with XbaI restriction enzyme. In this study the allele frequencies were found as follows: C: 0.84 and 0.808; T: 0.16 and 0.192. Based on result of the present study no significant impact of polymorphisms on production parameters was found.