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Development of an InDel marker set to establish hybridization between wild boar and domestic pig (Sus scrofa) breeds
21-25Views:243Wild boar and domestic pig breeds belong to the same species (Sus scrofa), so they can easily have viable offspring. This could be a problem in preserving the genetic lines of wild boars, keeping clean the food industry from lower-grade hybrid boar meat, and „producing” ethically questionable trophies, too. The aim of our study was to develop a cost-efficient, fast, easy and accurate marker set which can separate the wild boars from hybrids and domestic pig breeds.
The InDel markers were developed using 59 full pig genomes of 17 different breeds (e.g. Duroc, Large White, Landrace, Mangalica, wild boar). Sequence differences between the genomes of wild boars and domestic breeds were identified in variant call files, and verified using the IGV software. Wild boar, mangalica and duroc specific primers to amplify the chosen InDel regions were designed using Primer3.
After preliminary tests five markers were chosen, three wild boar specific, one Mangalica specific and one Duroc specific one. Fluorescently labelled primers were used to make the valuation easier and more accurate with capillary electrophoresis instead of gel-electrophoresis. The markers were optimised individually and in multiplex conditions and tested in samples of 11 breeds.
In conclusion, a new, faster and cheaper set was developed to separate the wild boars from the hybrids and domestic breeds. Based on the preliminary testing on wild boars, duroc and mangalica breeds zero samples resulted false negative, so it is 100% accurate. In addition, it is a much more cost- and time-effective way than testing every single sample with STR sets.
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Effect of sex on some composition traits of wild boar (wild pig) meat
25-29Views:189The authors examined the nutrition value of the meat of shot wild boars (wild pigs) (n=66) from three wild boar enclosures with different feeding intensity and also the technological properties of the meat. Samples were taken immediately after the evisceration. Considering the storing and processing properties of game meat the samples were taken from m. serratus anterior. As for dry matter examination results, the highest values were measured in case of semi-intensively fed wild boars, then followed the data from the samples of intensively and extensively fed wild boars. The fat content from the meat samples of intensively and extensively fed wild boars proved to be lower while in case of the semi-ntensively fed wild boars it was higher. In females the dry matter content, while in males the fat content was higher. As for the protein content there were no differences in either the feeding groups or in the genders. It was only the water holding capacity of the samples from the meat of the females from semi-intensive feeding intensity wild boar enclosure that fell in between normal values.