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Application of the SDS-PAGE method for the characterisation of winter wheat flour quality
112-118Views:101The principle, development and importance of the SDS-PAGE method are presented in this article. The SDS-PAGE method has become one of the basic methods of molecular biological research, because it is widely applicable and its sensitivity is excellent in the separation of wheat storage proteins.
We have shown the application of this method with a concrete example. It was also tested whether, it was possible to obtain a better baking quality product from a large amount of poor quality less valuable wheat by fractioning the flour according to particle sizes after grinding. We studied the rheological properties of flours with different particle size fractions from the original flour. The baking quality of the original flour was B2. The 125-90 and 90-63 μm fractions have significantly better baking quality (B1) than the original flour. The protein contents of these flour fractions were also significantly higher than the protein content of the original flour. We had a question: what has influenced the baking quality: the protein content or other factors? We searched for an explanation on these results in the protein composition of the flour samples. We studied the distribution of glutenin-fractions by SDS-PAGE method and evaluated them. We found with correlation analysis that the amount of LMW-Glutenin D-group (52-60 kDa) is in a strong, negative correlation to the baking quality (r = – 0.855*). Therefore, the baking quality of flour samples was influenced by this glutenin fraction. -
Study of effects on quality of different wheat flour fraction
123-129Views:96Wheat is one of the most important cereals in the world and the bread made of its flour belongs to the everyday life of human mankind.
The Hungarian standard relating to the laboratory production of wheat flour (MSZ 6367/9-1989) does not mention the type of laboratory mill used for milling, and it only builds up some general criteria, such as: the laboratory mill should be provided with four differently nicked barrels, a sieve with appropriate hole sizes, and also with the separated collections of the pilot flour and the bran. Our study was started at this point and the answers for the following questions were aimed to be found: do the flour patterns studied and produced with different grinding and sieving
techniques, widely used in laboratory mills of the same wheat pattern show any alterations after the impact of the formula production as regards chemical constitutions and reologic parameters. Various flours and whole grains of the wheat patterns sieved with different particle sizes were studied in this experiment. In producing this pattern two different mill types of FQC 109 and CHOPIN CD 1 as well as two different grinder types such as PERTEN 3100 and type of RETSCH 200 were applied. There were 3 different corn sizes of 160; 250; 800 μms used in the partition of the fractions. To study the differences the following measurements were conducted: dry matter, ash, protein content, wet gluten content, gluten index, gluten expansiveness, farinographic value, falling number and amilographic rate.
The results this research confirm that the quality of wheat flour can be modified by different methods of pattern production. In all cases the differences can be explained by the flour-bran ratio, and in some of the cases the higher germ content of the fractions also played a role. The results show differences between the various types of mills and grinders, too. -
The influence of fraction size on the chemical composition of winter wheat flour
123-126Views:84Wheat is one of the most important cereals in the world and the bread made of its flour belongs to the everyday life of human mankind.
The Hungarian standard relating to the laboratory production of wheat flour (MSZ 6367/9-1989) does not mention the type of laboratory mill used for milling, and it only builds up some general criteria, such as: the laboratory mill should be provided with four differently nicked barrels, a sieve with appropriate hole sizes, and also with the separated collections of the pilot flour and the bran. Our study was started at this point and the answers for the following questions were aimed to be found: do the flour patterns studied and produced with different sieving techniques,
widely used in laboratory mills of the same wheat pattern show any alterations after the impact of the formula production as regards chemical constitutions. Various flours of the wheat pattern sieved with different particle sizes were studied in this experiment.
In producing this pattern we used FQC109 type of mill. There were 5 different corn sizes of 250-200; 200-160; 160-125; 125-100; <100 μms used in the partition of the fractions. The results this research confirm that the quality of wheat flour can be modified by different methods of pattern production. -
Effects of ingredients and processing conditions on the quality frozen dough bread made from diferent wheat flour
115-120Views:67A laboratory scale no-time frozen dough procedure that approximates Romanian commercial practice has been
developed and used to study the effects of ingredients and processing conditions on the bread quality of a straight
grade wheat flour during prolonged storage (2 days to 26 weeks). All treatments (baking absorption level, mixing
energy input, mixer type, fermentation and intermediate proof times, removal of oxidant and/or dough
strengthening conditioners and partial freeze-thaw cycles) had significant effects (P<0.05) upon bread quality (loaf
volume and/or bread score). In general, these effects were more pronounced with extended frozen storage time.
High baking absorption, undermixing, bulk fermentation (> 1 h) and removal of oxidant and/or surfactants had
the most dramatic effects. Addition of a very strong flour at 30% to strengthen the wheat flour had no significant
effect (P>0.05) upon bread quality under optimum conditions. -
Effect of weather conditions on the protein content and baking value of winter wheat flour
83-94Views:110We searched for connections between weather conditions (with its sub-parameters as precipitation and average temperature) and the yearly formation of two quality parameters (protein content and baking value) on three levels of mineral fertilization, based on the results of a variety comparison experiment on chernozem soil, to select those weather parameters and critical periods which have significant effects on the quality of winter wheat flour.
We established that the protein content of winter wheat flour can be increased with increasing levels of mineral fertilizers. Protein content is lower and has higher deviation during non-fertilized conditions in different cropping years than on higher fertilization levels. Thus, it seems proved again that quality (as protein content) is mainly formed by the crop year, but can be improved with adequate agricultural engineering (with mineral fertilization in the present case). The higher sum of precipitation in May, and the lower average temperature after flowering, have the highest increasing effect on the protein content of flour of the examined parameters. Based on the results of the examined period, the rainier and warmer term than average before flowering and lower – average amount of precipitation and colder circumstances are favourable for higher baking values. The analysis with data of decades, proves the importance of the first half of May and the middle of June as especially important periods for quality formation. An increasing nutrient supply has different effects on the varieties; mineral fertilization increased the baking value of GK Öthalom winter wheat variety in almost every case, but the second level of fertilization decreased it in half of the examined years. Additionally, mineral fertilization played a role in the stabilization of the quality of highlighted varieties. -
Changes of Quality of Winter Wheat During Maturing
105-112Views:84We analysed the flour of two winter wheat varieties to determine the effect of maturity state and grain position in the ear on the element composition of flour. Samples were taken from the 14th to the 42th day of anthesis. Ear samples were cut into three (basal, central and apical) parts, the trashed grains were milled, and the element compositions of the flours were determined.
We found that the grain position within the ear has an effect on flour element content. Flours of apical grains had lower nitrogen, phosphorus and sulphur contents than flours of central and basal grains. The nutrients can be divided into two parts, based on the formation of element content during maturation. Nitrogen, sulphur and copper contents changed continuously during maturity, while the P, K, Ca, Mg, Mn, Sr and Zn contents decreased until a specific value and became nearly constant in the second half of maturity. We found that – although the nitrogen concentration of flours changed continuously – the amount of nitrogen in the flours reached a constant value for the second half of maturity. -
Overview of test methods used to classify wheat flour and bread samples – REVIEW
27-34Views:53In Hungary, common wheat (Triticum aestivum ssp. vulgare) is of good quality and world famous. In addition, it plays an important role in the human diet. The classification of flours ground from wheat is quite decisive and there are several methods for its examination. The most important flour testing requirements include moisture content, protein content gluten properties and the most important bakery value number. The measured characteristics give us the opportunity to conclude about the properties of the dough, and then bakery products. Several dynamic and static methods have been developed to study the physical properties of dough. The evaluation of products can be carried out in several respects with the help of a baking test. The multitude of methods currently used to qualify flour, dough and finished products also proves that the overview of the methods is quite topical.
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Stability analysis of different winter wheat genotypes in long-term experiment
135-141Views:86Nowadays, due to the climate change, it is becoming increasingly important in the occasionally extreme years that the yield and the quality
parameters of a given winter wheat variety should not fluctuate at all or only slightly under similar agrotechnical conditions as a result of the year effect. In four years (2005–2008) we studied the changes in the wet gluten content, gluten speading and protein content of five wheat genotypes at six fertilization levels.
In the control, it can be observed that the year had a significant effect on the wet gluten content, the protein content of the flour and gluten speading, therefore, a great fluctuation was detected in these qualities of the varieties in the four studied years. At the optimum fertilization levels (N120-150+PK), the varieties Sixtus, Saturus and Lupus showed a much lower fluctuation and more stable values were measured. The most stable variety in the control treatment was Mv Mazurka for all the three quality parameters, while at the optimum fertilization level (N120+PK), the most stable results were obtained for the variety Sixtus as an average of the four years.
When studying the results using Kang’s method for stability analysis, it can be stated that the most stable values of wet gluten content were obtained at the fertilization level of N120+PK under a variable year effect, the varieties gave also the best gluten content values at this level. The most stable protein content values of flour were obtained at the fertilization level of N60+PK. The results showed that the fluctuation of quality parameters as a result of the changing years differed between the different winter wheat varieties due to their differing genotypes, but this fluctuation could be reduced or minimized by a proper fertilization. -
Examination of wheat rheological characteristics by near-infrared spectroscopy
99-104Views:124Near-infrared spectroscopy has many advantages that make it a widely used analitical method in the different areas, like agricultural and food industry as well. In wheat quality control rheological characteristics of dough made from wheat flour are as important as physical and chemical properties too. In this work we examined rheological properties of wheat flour samples by alveograph, and spectral data of the same samples were collected by FOSS Infratec 1241 instrument. Modified partial least squares analyses on NIR spectra were developed for two alveograph parameter (P/L és W) to get calibration equations.
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Research on rheological characteristics of winter wheat varieties
266-272Views:113The gluten examination test is dominant in Hungary, comparing it to the qualification system of other countries. The determination of alveographic parameters is a basic criterion of winter wheat specifications in some western and southern European countries.
In the Hungarian and foreign winter wheat qualification systems, in the standards, there is no limit for extensographical parameters. Customers dictate the limits and make their claims as to the specifications.
We analyzed the alveographic and extensographical parameters of 19 winter wheat varieties grown by the Cereal Research Non-Profit Company in Hungary, and we made a comparison between the results in the challenges of the European Union expectations.
Examining the information of alveographic and extensographical values, we found that the experimental varieties provide high base to flour types suitable for baking bread and baker’s ware. The GK Élet, GK Petur, GK Memento, GK Csillag, GK Kapos and GK Marcel varieties can meet the market of paste flour needs, too.
In the variety series, the GK Kalász represents the highest values and the GK Garaboly shows the lowest parameters regarding the alveographic W and the resistance to extension.
On the basis of relation, we can establish that we can estimate the following little known and used quality indexes: the extensographical resistance to extension with extensographical energy and the alveographic W value, the extensographical energy with the alveographic W value and the alveographic P and L values with the other alveographic parameters. -
Examination of different grain hardness varieties of the grinding technology properties of wheat
423-435Views:173In the mill industry, the purpose of the grinding technology is to separate wheat endospermium and shell and to recover grists. The most important and the highest energy requirement operation is shredding. Flour quantity and quality produced from wheat depends on the variety of wheat that will be shredded, the type of grinding equipment and the condition used before the grinding. For this reason, during my experiments, I ground two grain structured varieties of wheat in laboratory conditions using a disk grinder, stone grinder and roll grinder in air-dry and conditioned states. We measured the equipment’s performance and the produced grist’s particle size distribution, followed by the calculation of the energy requirements of the grinders. In the grinding experiments we compared the ash contents of the different particle sized grist’s fractions to map the wheat’s particular properties.
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Use of extensigraph in the examination of wheat flours
17-23Views:119The extensograph is widely used in quality control and research laboratories studying wheat flour quality. The most commonly used measurements include Rm, the maximum resistance (maximum height of the curve); R5 the resistance at a constant extension of 5 cm; E, extensibility (total curve length); Rm/E, the ratio of maximum resistance to extension; and A, the area under curve. The extensograph has proved useful in the classification and assessment of flours on the physical dough properties. Another important application is its use in wheat breeding programs. Furthermore, extensograph is an excellent tool to study the effects of a wide range of ingredients on dough properties of wheat flour (like oxidants, enzymes, salt and additives), and it can also be used measure the changes during the frozen storage. In the present study we provide a review of the scientific literature concerning the possible applications of the extensograph.
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Investigation of the production parameters, nutrient and mineral composition of mealworm (Tenebrio molitor) larvae grown on different substrates
129-133Views:178During the rearing of mealworm (Tenebrio molitor) larvae, the optimisation of the growing substrate has a particular importance. The application of the appropriate substrate is a fundamental pillar for intensive and safe production. The requirements for substrate include the lack of toxins, high nutrient and micro-macro element content. The aim of this study was the evaluation of the effects of different substrates on the production parameters, nutrient and mineral composition of the mealworm larvae. The experiment lasted through 14 days. 5 treatments were set up at, where the variable was the substrate. The test system consisted of 25 units (5 treatments and 5 replicates). 10 mealworm larvae per unit, (total of 250 larvae) were used at the beginning of the experiment. Regarding the substrates, our study included sweet potato (SP), may turnip tuber (MT) and may turnip leaf (ML). In addition to the plant by-products, wheat flour (WF) and wheat semolina (WS) were used as control substrates. Trace element uptake and production parameters of the larvae were determined at the end of the experiment. Regarding the production parameters, it can be stated that the wheat semolina (0.081 ± 0.005 g) and wheat flour (0.069 ± 0.007 g) substrate used as control gave the best results for the final body weight. In terms of plant raw materials and by-products, sweet potatoes (0.063 ± 0.007) can only be recommended as substrate, while may turnip tuber and may turnip leaf produced significantly lower final body weight results (MT=0.034 ± 0.002 g ; ML= 0.036 ± 0.002 g). The nutrient composition of the larvae was not affected by the substrate, these results confirmed the high protein and fat content reported in the literature. The results with the production parameters were contradicted by the micronutrient content. Larvae reared on may turnip leaf (ML) and tuber (MT) showed the highest values for most of the macro- and microelements (potassium, calcium, magnesium, iron, zinc, copper) tested.
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Wheat cleaning and milling technologies to reduce DON toxin contamination
89-95Views:163Mycotoxicosis caused by Fusarium fungi holds a huge risk considering economic and food safety issues worldwide. By applying milling technologies, we attempted to reduce the concentrates of DON toxin, as it is the most often found toxin in wheat.
The processes of sieving, aspiration and combination had been used on wheat with high DON toxin concentration. As a next step, grains were sorted using a horizontal cylinder separator, assorted by an optical and a gravity separator, and finally, the products were scoured and ground. The contamination level of the wheat and flour samples were defined by the HPLC-MS method.
Regarding the results, it can be stated that toxin concentration was most effectively reduced by optical separation and scouring, and by applying these milling techniques, food safety can be increased significantly.
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Búzalisztek minőségének becslése tészta nyújtás-szakítás alapján állományvizsgáló műszerrel
38-44Views:75Literature experience was utilised to develop a method for the extensograph analog testing of paste with a QTS25 Texture Analyser. 54 wheat flour samples from the same variety from variety-preservation experiments at the Szeged were tested with this method. A study was made to establish whether the method is suitable for the estimation of results derived by traditional classification (trial baking, farinograph and gluten tests).
A close and significant correlation was found between the hardness and area data measured with a QTS25 micro-extensograph, the farinograph values and the gluten spreading data, and also between the stretching index (BC/AC) and the water uptake capacity. These correlations correspond well with the findings from a previous experimental series on industrial flour samples. A close and significant correlation was likewise observed between the grain hardness established by the MININFRA method and all the QTS25 micro-extensograph data. In contrast with earlier QTS25 data, however a close correlation was not found between the QTS25 data and the loaf characteristics.
With the use of micro-extensograph data, the estimation of the baking industry value has been improved through stepwise variable selection, with the inclusion of several variables. The estimation of loaf volume could also be solved by means of stepwise variable selection with the inclusion of QTS25 and MININFRA data. -
The analysis of flour blends as affected by the behaviour of two different quality flours of triticale under different fertilizer treatments
5-8Views:160Triticale is likely used in many countries in human consumption, due to its advantageous agronomical and nutritional properties mostly in blends. The baking quality of blends depends not just on the proportions of the used flours but also on their individual quality what can be influenced by fertilizer treatments.
22 flour blends were prepared with commercial wheat flour and triticale wholemeal flour in proportions from 0% to 100%. The triticale was treated with different amount of fertilizers (N30P30K30, N60P0K0). Changes of wet gluten contents and extensograph parameters of the blends were determined. The quality of blends significantly depends on the fertilizer treatment of triticale beside the proportions of the flours. When the N60P0K0 treated triticale was used in blends, wet gluten and extensibility values were significantly higher, but in case of resistant to extensions (Rmax) the N30P30K30 treated samples gave higher values. The measured values of wet gluten and extensibility were above the expected values (synergism), while in the case of resistance to extension the expected values were higher than the measured values (antagonism).
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Effects of cultivar and fertilization on extensograph parameters of winter wheat flours
19-24Views:95In present paper we have examined the effect of mineral fertilization on the extensograph characteristics of wheat-flours. The baking quality of winter wheat is largely determined by cultivar, but it can be influenced by fertilization. Flours were from 4 cultivars grown at six nitrogen fertilizer. The average flour protein content of cultivars was very different (11.8, 13.0, 14.3, and 13.07%, respectively). We have found that the extensograph
properties of dough are affected by mineral fertilization, nevertheless, different cultivars distinctly react to the increase of fertilizer doses. We estimates the correlation between flour protein content and extensograph properties, based on our investigations, it became obvious that the correlation between flour protein content and extensograph parameters is really strong. -
New possibilities to determine wheat quality parameters by near-infrared spectroscopy
65-69Views:139Analyses and methods in wheat quality determination require more sample, time, work and cost, thatswhy flour quality control needs rapid, reliable tools. Near-infrared spectroscopy has many advantages, which make it suitable in quality control. NIR instruments need calibrations to their work. In our study we examined gluten content, falling number, valorigrpahic waterabsorption, alveographic P/L and W value of wheat samples. Modified partial least squares analyses on NIR spectra were developed for each property. The results show that we got such calibration modells, which are able to predict the properties (expect falling number) with enough accuracy.
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The effect of climatic change on the rheological properties of winter wheat doughs
96-100Views:77In present paper we have examined the effect of climatic change on the extensigraph characteristics of wheat-flours. The baking quality
of winter wheat is largely determined by cultivar, but it can be influenced by weather conditions during growing period. Flours were from 5
cultivars grown at one location in three cropping years. We have found that the extensigraph properties of dough are affected by the weather
conditions, nevertheless, different cultivars distinctly react to the increase of temperature and decrease of precipitation. In generally, the
higher average day temperature and lower precipitation level is favourable to produce winter wheat flour with better quality -
Examination of extensographical parameters of winter wheat (Triticum aestivum) flour
109-115Views:107In the trade of the European Union principally the analysis of alveographical and extensographical parameters mean the acceptance system.
In the present study we analysed the extensographical parameters of 10 winter wheat varieties breed by the Cereal Research Non Profit Company with Brabender extensigraph and we made a comparison, correlation among the results. The examinations with Brabender the following among: show the GK Kalász and the GK Élet varieties show high resistance of extension and the GK Petur variety produces high extensibility. The GK Garaboly has shown low extensibility and energy. The other parameters had different values in the examined three years. The measurings with SMS2 Texture Analyser show middle positive correlation between extensibility and subarea. There are weak correlation between at the significantial level in the 90, 135 resistence of extension by Brabender and by SMS2 Texture Analyser and in the 45 extensibility by Brabender and by SMS2 Texture Analyser of relaxed dough. There is no correlation among other parameters. -
Surface polishing method application to reduce micotoxin content of mill wheat
339-342Views:138The fungi causing the infection and most of the harmful toxins they produce are concentrated in the bran of the grain, thus the intensive surface cleaning, the so-called debranning operation could allow the reduction of contamination in the milling technology. The essence of the PeriTec technology – originally developed by SATAKE, a Japanese company, to clean rice – is that it gradually removes the bran layers of the grain by mechanical means before further processing. We modeled the PeriTec technology with a laboratory size, batch-operating, horizontal debranning machine by SATAKE. The flour, milled grain after grinding 40 sec, the initial toxin content was only a small proportion (~15–20%) measured. The results showed that below the limit of DON toxin contaminated wheat (DON: 1.15 mg kg-1) during the grinding surface of the detached bran toxin contamination shows a very high (6.16 mg kg-1). The 40 seconds debranning before grinding shows lower DON toxin content than without debranning. So it is importance before the grinding. The toxin contamination of the bran fractions is significantly reduced, which is importance to the feeding point. As a result of debranning, the toxin content of the grinding fractions decreased, which justifies that that PeriTec method is suitable for the reduction of toxin contamination.
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Comparison of alveohraph and extensigraph properties of winter wheat samples
27-30Views:100The rheological properties of dough are determined by the amount and proportion of gliadin and glutenin proteins. Extensibility and resistance to the extension of doughs which can be measured using the extensigraph or alveograph allow good assesment of the baking behavior under realistic conditions. In our study, we compared the extensigraph and alveograph parameters of 87 flour samples. The results showed that the alveograph parameters were different from the extensigraph parameters. The classification of wheat varieties was different, and the order of varieties, as well. The alveograph properties were affected by other factors than the extensigraph properties, except the W value. The two rheological tests did not give similar results, therefor neither test are replaceable.
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Comparison of chemical parameters of enzyme active and inactive malt types
161-166Views:199Nowadays there is an increasing emphasis on the use of raw materials. Typically, raw materials – in this study malt – are used in animal feeds and used in the brewing industry. However, in terms of quality (eg. high fibre content), these can be included in human nutrition, we have limited information on this possibility. The aim of our work was to compare different malt flours and examine the possibility of using malt in the baking industry. We were to investigate some of the most relevant parameters, such as dietary fibre content, crude protein content, fat content, carbohydrate content, dry matter content, moisture content, salt and energy content. In the future, we aim to conduct a research on some of these parameters with different malt types as the brewing industry uses novel ingredients different cereals, pseudocereals such as amaranth (Amaranthus spp.), oat (Avena sativa L.), quinoa (Chenopodium quinoa Willd.) in addition to the spring barley (Hordeum vulgare L.) or wheat (Triticum aestivum L.). Based on brewing studies, malt has a high fibre and protein content. Having these advantageous qualities, malt should be part of humans’ healthy diet. Using malt flour in the baking industry can be a new direction which can lead to creating a healthier lifestyle and healthier eating habits than suggested by the WHO (World Health Organization).