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The impact of different fertilization methods on some microbiological soil characteristics
119-126Views:93In our experiment, we studied the impact of an organic fertilizer, Bactofil® A10 (half- and full dosage applied in field practice) and an artificial fertilizer of Ca(NO3)2 content in different dosages (20-40 mg kg-1) – in addition to control treatments – on two different soils (calcareous chernozem, humus sandy soil) in 2005-2006, the experiment was complemented with treatments applying 250% dosage (100 mg kg-1 N, Bactofil® A10 2.5 times the field dosage) and a compost from urban sewage (25 g kg-1 compost) was also tested on these two soil types. In the
experiment, several soil microbial parameters were studied. The experiment was set up at the Department of Agrochemistry and Soil Science using 1-kg pots.
Our laboratory experiments were performed at the soil microbiology laboratory of UD CAS Department of Agrochemistry and Soil Science, the total number of bacteria, microscopic fungi, nitrifying and aerob cellulose-decomposing bacteria were determined together with the CO2-production of soil, N content of the biomass and urease enzyme activity.
Statistical analysis of the data was done using the program SPSS 13.0, means of the measurements, deviation and significance values were calculated.
In 2005-2006, the effect of the different dosages of Bactofil® A10, and the Ca(NO3)2 fertilizer on the examined microbial parameters of calcareous chernozem and humus sandy soils can be summarized as follows:
• Concerning the total number of bacteria, both treatments were effective on calcareous chernozem soil, the higher (significant) increment in bacteria number was observed in the artificial fertilizer treatments, while in the humus sandy soil Bactofil treatments had a beneficial effect. The number of microscopic fungi also increased in both treatments, higher numbers were observed in the average of two years in the Bactofil treatments.
• The number of nitrifying bacteria was 2.5 times higher in both high-dosage treatments on calcareous chernozem soil, while on humus sandy soil a slight (not significant) increment was observed only int he high-dosage Bactofil treatment. The amount of aerob cellulose-decomposing bacteria significantly increased on calcareous chernozem soil in both the highdosage artificial fertilizer and the small-dosage Bactofil treatment, however, on humus sandy soil no significant increase was observed in either treatment.
• The CO2-production increased in both soil types, although it was not significant in either treatment. A higher (though not significant) soil respiration was observed in the Bactofil treatments in both soil types.
• The microbial biomass N values were significantly higher in the high-dosage Bactofil treatments, however, the high-dosage artificial fertilizer treatment also increased these values significantly on calcareous chernozem soil.
• On calcareous chernozem soil, urease activity was significantly increased and reduced by high-dosage artificial fertilizer treatments and Bactofil treatments, respectively. On humus sandy soil, urease activity was also reduced except for the high-dosage artificial fertilizer treatment. In 2007, the pot experiment with 250% dosages was complemented with the application of compost rich in organic matter, the results of these treatments are sumnmarized as follows:
• In the case of the total number of bacteria, all three treatments resulted in a significant increase on calcareous chernozem soil with the highest values in the Bactofil treatment. The Bactofil treatment was the most effective on the humus sandy soil, but the artificial fertilizer treatment also
resulted in a significant increment. In the case of the total number of fungi, Bactofil treatments resulted in the highest values on both soils, but the compost treatment also increased the number of fungi in calcareous chernozem significantly.
• The number of nitrifying bacteria was increased most (significantly) by the Bactofil and compost treatments on both soil types. The amount of cellulose-decomposing bacteria was significantly increased by he compost treatment on calcareous chernozem soil, while its effect was not significant on humus sandy soil. The number of these bacteria was increased significantly by the Bactofil treatment on humus sandy soil.
• On calcareous chernozem soil, all three treatments significantly increased CO2-production, while the compost treatments had the resulted in the largest increment in soil respiration on both soil types.
• The soil biomass N content was significantly increased in both soils by the compost treatment, while in the case of the humus sandy soil, the Bactofil treatment also resulted in a significant increment.
• Urease enzyme activity was significantly increased by the artificial fertilizer treatment on both soils. In calcareous chernozem soil, the Bactofil treatment resulted in a slight (not significant) reduction in enzyme activity. In humus sandy soil, the Bactoful treatment also resulted in a slight reduction, while the compost treatment increased (though not significantly) the urease activity.
Based on our results, it can be stated that all three treatments were effective with respect to the studied soil microbial parameters. For both the calcareous chernozem and the humus sandy soil, the organic fertilizer Bactofil and the compost with high organic matter content had a stronger effect on some soil microbial parameters than the artificial fertilizer. -
Comparative examination of a mineral fertiliser and a bacterial fertilizer on humic sandy soil
111-116Views:79In our pot experiment, the impact of a bacterial fertilizer, Bactofil® A10 and a mineral fertilizer Ca(NO3)2 applied in different rates was studied on some soil chemical and microbiological characteristics of a humic sandy soil (Pallag). Perennial rye-grass (Lolium perenne L.) was used as a test-plant. Samples were collected four and eight weeks after sowing in each year. The experiment was set up in 2007-2009 in the greenhouse of
the UD CASE Department of Agrochemistry and Soil Science. The available (AL-extractable) nutrient contents of soil, among the microbial parameters the total number of bacteria, the number of microscopic fungi, cellulose-decomposing and nitrifying bacteria, the sacharase and urease enzyme activity, as well as the soil respiration rate were measured.
Statistical analyses were made by means of the measurements deviation, LSD values at the P=0.05 level and correlation coefficients were calculated. Results of our experiment were summarised as follows:
− The readily available nutrient content of humic sandy soil increased as affected by the treatments, in case of the available (AL-extractable) phosphorus and potassium content the higher value was measured in high-dosage artificial fertilizer treatment.
− The treatments had also positive effect on several soil microbial parameters studied. The higher-dosage mineral fertilizer treatments had a beneficial effect on the total number of bacteria, cellulose-decomposing and nitrifying bacteria. No significant differences were obtained between the effect of treatment in case of the total-number of bacteria, the number of microscopic fungi and nitrifying bacteria.
− On the sacharase enzyme activity the artificial fertiliser treatments proved to be unambiguously stimulating, the urease activity significantly increased on the effect of the lower-dosage Ca(NO3)2 artificial fertilizer treatment.
− The soil respiration increased in all treatments in related to the amounts applied, significantly increased in the highest rate of Ca(NO3)2 fertilizer addition.
− Some medium and tight positive correlations were observed between the soil chemical and microbiological parameters studied in case of both nutrient sources.
Summarizing our results, it was established that the organic and all the mineral fertilizer treatments had beneficial effects on the major soil characteristics from the aspect of nutrient supply. In majority of the examined soil parameters (AL-extractable phosphorus- and potassium, total number of bacteria, number of cellulose-decomposing and nitrifying bacteria, activity of sacharase enzyme) the high rate of Ca(NO3)2 mineral fertilizer treatment proved to be more stimulating, but at the same time the high rate bacterium fertilizer resulted in significant increases in
the nitrate-N content, the AL-potassium content of soil, the total number of bacteria, the number of cellulose-decomposing and nitrifying bacteria and the urease enyme activity.
Our examinations showed that the mineral fertilizer treatments proved to be more stimulating on most of the soil parameters studied but according to our results, it was established that Bactofil is efficiently applicable in the maintenance of soil fertility and the combined application of
mineral fertilizer and bacterium fertilizer may be a favourable opportunity – also in aspect of the environmental protection – in maintaining soil fertility. -
The effect of bentonite on the quantity change of soil microorganisms, the CO2-production and the activity of saccharase enzyme
287-293Views:74We examined the impact of bentonite – the perspective improving material of sandy soils – and treatments of livestock manure composted with bentonite on sandy soils, within the framework of a small-plot experiment.
The adjustment of the experiment was made on the Experiment Site of the Nyíregyháza Research Centre of the University of Debrecen, Centre of Agricultural Sciences (UD CAS). We collected soil samples from parcels treated with increasing bentonite doses (5, 10, 15, 20 t/ha) on the one hand, and from the parcels treated with livestock manure composted with increasing doses of bentonite, on the other.
We performed laboratory research in the soil microbiology laboratory of the Soil Science Faculty of UD CAS DAS, during which we determined the total number of bacteria, the quantity of microscopic fungi, the number of cellulose-decomposing bacteria, the CO2-production of the soil and the activity of saccharase enzyme.
During the evaluation of the examinations, we made a statistical analysis using SPSS 9.0. We determined the average of measurements, the standard deviation of controls, the standard deviation, the significance value and we also performed a correlation analysis.
Concerning the impacts of bentonite treatment and the treatment of livestock manure composted with bentonite on the examined microbiological features of sandy soil, we can summarize the following:
• Our results prove that the microbiologic activity of the soil has increased owing to the impact of bentonite treatments regarding total number of bacteria and the quantity of microscopic fungi. ”Pure bentonite” treatments – although not significantly in every case – increased these values, but larger doses decreased them. The treatments of livestock manure composted with bentonite resulted in a larger increase regarding both parameters.
• The number of cellulose-decomposing bacteria was increased by the low doses of both series, and was decreased by the higher doses. Higher bentonite doses decreased it in a higher – significant – degree than those of treatments of livestock manure composted with bentonite, whose low dose caused salient number of bacteria.
• Regarding the carbon-dioxide formation, we have experienced an increase even in the case of low dose treatments (nevertheless, the increase did not prove to be significant), but – similarly to the quantitative changes in the number of cellulose-decomposing bacteria – the large doses of both series of treatment decreased the CO2-production of the soil.
• Bentonite also increased the activity of saccharase enzyme significantly. We learned that”pure bentonite” treatments increased the activity of the enzyme to a higher degree than composted treatments. Moreover, it can be stated that the treatments of larger doses of both bentonite and livestock manure composted with bentonite have decreased the enzyme activity – not significantly, though.
• Based on the correlation analysis, it can be stated that as an impact of the treatments, the microbiological activity of the soil has also increased with the increase of the number of soil microbes, as in both treatment series we have experienced a tight positive correlation (r=0.81-0.82) between the change of total number of bacteria and the CO2-production of soil. In both treatments, there was a medium correlation between the total number of fungi and soil respiration (r=0.63-0.63). Furthermore, it can be stated that the usage of organic manure had a positive effect on the activity of cellulose-decomposing bacteria, as in this treatment series there was a positive correlation (r=0.65) between this physiological group and CO2-production. Both treatments prosperously impacted the activity of saccharase enzyme, because there was a medium correlation (r=0.62-0.64) between the activity of the enzyme and soil respiration. -
Impact of the integration of lupine (Lupinus albus) into crop rotation on the extent of soil compaction in the Westsik longterm field trial
529-537Views:89In order to reduce or eliminate soil compaction, rational crop rotation and appropriate sequence of crops have an increasingly important role in addition to mechanical and tillage solutions. In this respect, introduction of greening in recent years has been a major step, which focuses on aspects of environmentally conscious, soil conserving farming and the improvement of biodiversity. The cornerstone of this strategy is the cultivation of crops that have a beneficial effect on soil properties, such as the use of nitrogen-fixing plants and green manure plants in the cultivation system that have a beneficial effect on soil structure. In our examinations, penetrometer measurements were carried out in the second longest crop rotation-based field experiment in Europe in order to quantify the effects of green crops and crop rotation strategies on soil resistance. Our aim was to evaluate and compare the impact of lupine (Lupinus albus) on the penetration resistance of soil on sour sandy soils. At the time of the penetration resistance measurement, different crop rotations had a significant effect on the development of the parameter in the examined soil layer. The most favourable penetration resistance values were found in the crop rotation, which included lupine as a green manure. The favourable effect is dominant below the cultivated layer (0–40 cm), which is statistically verified. The values of penetration resistance of the cultivated soil layer of lupine sown as primary green manure did not differ significantly from the values measured in the case of the fallowing-based crop rotation. Therefore, the use of lupine green manure instead of fallowing could be worth considering by practical application due to its favourable effects on soil penetration resistance. The use of lupine green manure after the production of rye cultivation resulted in penetration parameters similar to fallowing, irrespective of the green crop and the applied amount of nitrogen fertilizer, which justifies the cultivation of the crop as green manure. In the case of potato cultivating, recorded compaction within the cultivated layer is an obvious consequence of mechanical compaction during harvest; therefore, machinery operations are decisive for the development of penetration resistance values of the cultivated layer. In addition to the beneficial effect of lupine as a green manure crop on soil condition, its nitrogen-fixing ability is also important; it stresses the utilisation of the crop of sour sandy soils for the sake of proper soil management.
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The Effect of Soil Coverings on Soil Respiration in Sandy Soil
21-25Views:121The purpose of our experiments is to study effect of different soil coverings (porous black polyethylene called agroszövet and black polyethylene) on CO2 production in sandy soil. The CO2 production was measured in our laboratory according to Witkamp (1966 cit. Szegi, 1979), after 5 days’ incubation period. Samples were taken off four times (March, May, July, September) in every year of the experiment. In May, July and September of 2000, the CO2 production was significantly higher in the control than in the treatment soil. With the exception of September, the value of CO2 production was significantly higher under black polyethylene than under agroszövet. In March and May of 2001, the soil under black polyethylene, and in July and September the control soil produced the greatest quantity of CO2. With the exception of July, significantly more CO2 was produced under black polyethylene than under agroszövet. To study the dynamic of CO2 production there was find a significantly higher value May and September of 2001 than 2000. Similarly significant higher CO2 production was detected in September than in the other months In average of two experimental years the difference between the produced CO2 under different coverings was occasionally. Explicit upward tendency in soil CO2 production was detected only in case of control soil. There was a medium (r=0,413) relationship observed between the moisture content and the CO2 producing ability of soil. To sum up the soil coverings had favourable effect on soil CO2 production very rearly, but they could help to conserve the moisture content of soil.
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The application of bentonite and zeolite for soil amelioration in acidic sandy soil
131-137Views:103In a pot experiment, we have studied the effect of bentonite and zeolite in different dosages [control; 5; 10; 15; 20 g kg-1] on acidic (pHH2O=5.65) humus sandy soil. The experiment was set up in 2007 and 2008 in the greenhouse of the UD CASE Department of Agrochemistry and Soil Science. As a test plant, perennial ryegrass (Lolium perenne L.) was used.
In laboratory examinations, pH(H2O), pH(KCl), hidrolytic acidity, nitrate-N content, readily available phosphorus and potassium content were determined. Among soil microbial parameters, the total number of bacteria, the cellulose-decomposing bacteria, the carbon-dioxide production, the microbial biomass-C content of soil, and the saccharase enzyme activity were measured. In the experiment the biomass of the test plant was determined.
The effect of bentonite and zeolite in different dosages can be summarized as follows:
− The pH increased under the effect of low dosages. With the increasing of the pH the hydrolytic acidity - at the bentonite treatments significantly – decreased.
− Regarding the readily available nutrient content of the soil, low and medium dosages proved to be effective. High dosages of bentonite treatments reduced the nitrate-N content, the readily available phosphorus, and potassium content of soil, by zeolite treatments the high dosages reduced the nitrate-N content of soil.
− Regarding the measured soil microbial parameters in both treatments low and medium dosages proved to be also effective, but the high dosages didn’t cause decreasing at the total number of bacteria, and by zeolite treatments the biomass-C content of soil.
− Also the bentonite and zeolite treatments enlarged the biomass of the test plant. We experienced significant increasing by bentonite treatments by the effect of medium and high dosages, while in zeolite treatments only the high dosage caused significantly increasing in plant biomass. The largest dosages decrease the plant biomass.
− Under the statistical analysis we found many medium and tight correlation between the studied parameters. -
Impact of ammonium nitrate and Microbion UNC bacterial fertilizer on dry matter accumulation of ryegrass (Lolium perenne L.)
35-39Views:80Pot experiment was performed to investigate the effects of increasing NH4NO3 doses with or without Microbion UNC bacterial fertilizer
application on dry matter production of ryegrass (Lolium perenne L.). Experiment was set up on calcareous chernozem soil of Debrecen-Látókép and on humus sandy soil of Őrbottyán. The bi-factorial trials were arranged in a randomized complete block design with four replications. Grass was cut three times. Dry matter production was determined and the sum of biomass of cuts was calculated as cumulated dry weights. Analysis of variance was carried out on the data in order to provide a statistical comparison between the treatment means. The least significant difference (LSD5%) test was used to detect differences between means. On the basis of our results it can be concluded, that the dry weights of ryegrass cultivated on chernozem soil were higher than on sandy soil. With increasing nitrogen supply the dry matter production of grass significantly increased in both types of soils. In case of sandy soil the increasing effect was more expressed, but dry weights of this soil never reached the appropriate values of chernozem soil. Application of Microbion UNC had positive effect on dry matter production of ryegrass grown on both two types of soils but the effect was more expressed on chernozem soil. Finally it can be concluded that the increasing effect of NH4NO3 on biomass weights was more expressed in both types of soils, the biofertilizer application also increased the dry weights of plant in a small degree. -
The Effect of Soil Coverings on Cellulose Decomposition Activity of Sandy Soil
15-20Views:66The purpose of our experiments is to discover the effect of different soil cover matter (agroszövet and black polyethylene) on actual (under field circumstances) and potential (under laboratory circumstances) cellulose decomposition activity. In our field research, the Unger-test was used, and in laboratory research, the Petkov-Markova method was applied. In the first year of the experiment (2000) actual cellulose decomposition activity was significantly higher in covered than in the uncovered soil both in spring and autumn. The difference between the two treatments was significant only in spring. In the spring of 2001 black polyethylene showed significantly the lowest, activity, while in autumn the agroszövet (a porous black polyethylene) showed significantly the highest activity. In the autumn of 2001 the soil covered by black polyethylene gave non-significant,and the soil covered by agroszövet gave a significant higher activity value than the control. Averaging the two experimental years (2000-2001), the actual cellulose decomposition activity was significantly higher in covered soils both in spring (with 30-39%) and in autumn (with 34-69%). Moreover, in autumn a significantly higher value was detected under agroszövet than in any other treatment. The difference between the effect of treatments was not significant. In 2000, the potential cellulose decomposition activity was the highest in soil covered by agroszövet in spring, but in autumn higher activity value was detected in every covered soil than in the control. In the spring of 2001, every covered soil showed a lower, but in autumn a higher, potential cellulose decomposition rate than the control. The activity decreased significantly 27 (agroszövet) and 45 (black polyethylene) percent in spring, and increased no- significantly 8 (agroszövet) and 4 (black polyethylene) percent in autumn. During the two experimental years, we observed on average lower potential cellulose decomposition activity (15-60%) in spring and a higher one (14-43%) in autumn. Neither was significant. The dynamic of potential cellulose decomposition activity averaging 2000 and 2001 showed a slight, the actual cellulose decomposition activity an explicit non-significant upward tendency. There was a strong (r=0,189) correlation obtained between the actual and potential cellulose decomposition activity of soil, and a medium-strong (r=0,673) relationship between the soil moisture content and actual cellulose decomposition activity.
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Influences of different organic fertilizers on nutrients of humic sandy soil and on the growth of Spinach (Spinacia oleracea L.)
23-28Views:292A greenhouse pot experiment was conducted to compare the effects of manure with different origin (horse, cattle), various bedding materials (straw, sawdust) and diverse doses (30 t ha-1, 60 t ha-1) and the impact of food waste compost on the plant growth and the available plant nutrient content of soil. The study was conducted on humic sandy soil and consisted of 9 treatments in a randomized complete block design with four replications. Spinach (Spinacia oleracea L.) was grown as the test crop. The treatments were: 1. unfertilized control; 2. horse manure with straw (30 t ha-1); 3. horse manure with sawdust (30 t ha-1); 4. cattle manure (30 t ha-1); 5 food waste compost (30 t ha-1); 6. horse manure with straw (60 t ha-1); 7. horse manure with sawdust (60 t ha-1); 8. cattle manure (60 t ha-1); 9. food waste compost (60 t ha-1). Plant growth was monitored for 4 weeks. Shoot and root weights per pot were measured, total biomass weight per pot were counted.
On the basis of the results it can be concluded, that among treatments the application of horse manure with straw enhanced spinach growth most significantly compared to other treatments and to the non-treated control, resulted the highest weights of leaves and roots of spinach. At the same time even small dose (30 t ha-1) of this fertilizer caused increased plant available nitrogen and phosphorus of soil and the higher dosage further increased these values. The horse manure with sawdust applied in lower dose did not alter the leaves and roots weights, but higher portion (60 t ha-1) caused significantly decreased plant biomass. The results proved that the bedding material may significantly alter the composition of manure and may change the plant nutrition effect of organic fertilizer. Cattle manure and food waste compost in both applied doses enhanced plant growth. Both fertilizers increased the plant available nitrogen forms and phosphorus content of soil, but cattle manure caused higher increase.
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A szervetlen-C és a szerves-C mennyiségének változása egy homoktalajban, meszezés hatására
8-10Views:91The effect of liming on different organic and inorganic carbon forms in soil was studied in a pot experiment on acidic sandy soil (pH(H2O)=4.38; pH(KCl)=3.42). We used three kinds of liming doses (which were signed M1, M2, M3,). M1 was half of the M2 dose; M2 was the calculated lime dose and M3 was one and the half dose of M2. Lime (CaCO3) was mixed into the soil in powdery form. In addition to liming treatments, a uniform N, P and K treatment (1-1-1g N, P2O5 and K2O per pot) was given. Water supply was set up to 75% field water capacity of soil. Pots were watered daily. During the vegetation period soil and plant (oats; Avena sativa L.) samples were taken from each treatment three times (after 6-10-15 weeks). We determined inorganic- and total-C of the soil by Vario EL element analyser (based on dry combustion method) and calculated the organic-C.
The conclusions can be summarized as follows:
– Liming treatments had a significant positive effect on the soil-pH and caused quantitative change of different carbon forms of soil.
– Increasing tendency of liming doses caused more intensive changes in inorganic- and organic-C forms of soil.
– A significant correlations was found between the soil pH and the inorganic-C fraction of soil.
– A decreasing correlation was found between the vegetation period and the organic-C fraction. -
Effect of soil-compost proportion on the abiotic and biotic parameters of soilplant system
99-104Views:95The environmental awareness, coming to the front in the 21st century, motivates us to supply the plant nutrient demand (in point of the plant, the environment and the human health) with natural materials.
Composting is known since the beginning of civilization. We came to know more the processes of composting as a result of last decades’ research, but numerous unexplained questions remained up to this day. The good compost is dark gray or brown, and it should not create an odor. It has aggregate structure, and it’s pH is neutral. Compost is soil-like (Fehér, 2001), nutrient-rich material, which contains valuable nutrients extracted from soil, so if we recycle this, we can decrease the chemical fertilizer and other (example: mineral energy) expenses.
The reason of that we chose the more accurate cognition of compost utilization is to do more effective the site-specific nutrient supply. This increases the average yield and the quality of yield. Besides we can decrease the harmful effects, which endanger the plant, the environment, and the human body.
During the compost utilization experiment we blended the acid sandy soil with compost in 4 different volumetric proportions (5 treatments) than we set the pots randomized. The advantage of this method is that we can provide equal conditions for plants so we can measure the effect of treatments correctly. Our experimental plant was ryegrass (Lolium perenne L.), that grows rapidly, tolerates the glasshouse conditions, and indicates the effect of treatments well. After the harvest of ryegrass we measured the fresh and dry weight of harvested leaves and the total C-, N-, S-content of the dry matter and of the soil, we examined the pH and the salt concentration of soil as well.
Our aim was to study and evaluate the relations between the compost-soil proportion and the nutrient content of soil and plant. In our previous experiments we confirmed (based on variance analyses) that the compost has a beneficial effect on soil and increases the nutrient content of the soil (Szabó, 2009). But it’s important to appoint that the compound of compost is seasonally change: in winter the selective gathered municipal solid waste contains salt that were applied for non-skidding of roads, but salt has a negative effect to the plant. We proved that in our experiment the 25/75% compost/soil proportion was ideal for the plant. This content of compost effected 6 times higher green matter weight compared to the 100% sandy soil. -
Effect of different sources and doses of sulphur on yield, nutrient content and uptake by spring wheat
109-115Views:126The objective of this study was to investigate the effect of two sulphur forms (sulphate and tiosulphate) in combination with three different N:S ratios on the yield of spring wheat and total N- and S-content and uptake by the aboveground biomass on chernozem and sandy soil. In the greenhouse experiment, the effects of two sulphur forms were compared: sulphate (SO42-) and thiosulphate (S2O32-). The sulphate was applied as potassium-sulphate (K2SO4) and thiosulphate as ammonium-thiosulphate ((NH4)2S2O3). Increasing doses of both sulphur forms (24, 60, 120 kg S ha-1) were used with the same nitrogen dose (120 kg N ha-1) which caused three different N:S ratios background (1:0.2, 1:0.5, 1:1). Nitrogen was supplied in the form of monoammonium-phosphate (MAP), ammonium-nitrate and ammonium-thiosulphate. Plant samples were taken in three different development stages of spring wheat based on the BBCH scale: at the stage of BBCH 30–32 (stem elongation), BBCH 65–69 (flowering) and BBCH 89 (ripening). The total nitrogen and total sulphur content of plant at different development stages and also wheat grain were measured by Elementar Vario EL type CNS analyser. The nutrient uptake by plant and grain was calculated from the yield of spring wheat and the N and S content of plant. The grain yield on chernozem soil ranged between 6.31 and 12.13 g/pot. All fertilised treatments significantly increased the grain yield compared to the control. The highest yield was obtained in the case of the application of 120 kg N ha-1 and 60 kg S ha-1in sulphate form. The grain yield on sandy soil varied from 2.53 to 6.62 g/pot. The fertilised treatments significantly enhanced the yield compared to the control. The highest yield was observed in the case of the application of 120 kg N ha-1 and 60 kg S ha-1 in thiosulphate form. On chernozem soil the increasing doses of sulphur (24, 60, 120 kg S ha-1) with the same N dose (120 kg N ha-1) increased the N-content of spring wheat at all development stages and in the grain. The treatments with different sulphur sources did not cause further changes in the N-content. On sandy soil in the most cases the N-content did not change significantly as a result of increasing sulphur doses. The treatments with sulphate form basically resulted higher nitrogen-content than treatments with thiosulphate form. The treatments with increasing sulphur doses resulted higher S-content on both of chernozem and sandy soil in the case of all development stage. Comparing the effect of the applied sulphur sources on the S-content it can be stated that at the stage of BBCH 30–31 and 65–69 the treatments with sulphate form resulted higher sulphur-content. At the stage of BBCH 89 there was no significant differences in S-content of grain as a result of different sulphur-sources.
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Yield components of hairy vetch (Vicia villosa Roth.) in different sowing technologies on acidic sandy soil
83-88Views:242Nearly a quarter of the agricultural utilized area of our country is made up of sandy soils. Sandy soils are poor in nutrients, and, therefore, the effectiveness of farming is basically determined by the method of maintaining soil fertility and the fertilization practice.
The hairy vetch called Vicia villosa Roth (Sandy Roth.), also known as a sand pioneer, plays a significant role in the exploitation of sandy soils. Its cultivation was started in Hungary in the late 1800s. It is primarily used as green fodder, most recently as a green manure and as a soil protection plant. The lupine is grown mainly as a supportive plant, which was previously rye, and today it is triticale. The ratio of the two plants to each other and the spatial location of plants depend on the method of sowing.
The aim of our work was to present the yields of some of the grain grown in different sowing methods and some of its crops.
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Effects of fermented chicken manure products on the N mineralization rate of the soil using the incubation method
199-204Views:130In our study, the effect of fermented and specially added poultry manure products (superabsorbent polymer (SAP), bentonite and Aegis as a mycorrhizal inoculum) were investigated in a short soil incubation experiment – at 60% water capacity level - on sandy soil. Soil samples were collected from two layers of the incubation pots after the second and fourth week to check the status of the tested products and the processes in the soil. The pH and the electric conductivity (EC) of the samples were measured using an electrochemical method, while the ammonium and nitrate content of the samples was determined with a photometric method. Soil pH and EC values slightly were decreased during the experiment. Our results pointed out that the increasing dose of SAP caused lower soil pH. The nitrate content of the soil did not change significantly during the experiment. It was found that the increasing SAP content in the products, due to its cross-linked structural property, protected the nitrate ions from leaching. Our results suggest that applied SAP does not bind the nutrient ions so tightly in its structure that it competes with the plant for uptake.
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Comparative studies to model bioavailability of pesticides in distinctive soil types
17-23Views:81Bioavailability of pesticides is determined by two major factors: soil characteristics and pesticides’ chemical feature. These factors result in a definite adsorption capability whose extent varies on a large scale. By revealing interactions between pesticides and soils it is of high interest to model bioavailability of widely used pesticides, as it is a key element in terms of prospective toxicological aspects. Our work signifies steps forward improving pesticide soil mobility prediction models as we created model systems representing correctly natural relations. Comparison of different solvent extraction methods proved to be an efficient tool to gain information on the bioavailability of some widely used pesticides as well as to model actual environmental processes.
Comprehensive comparison has been made between different experimental methods by applying 5 extraction models showing diverse efficiency in extracting capability of pesticides. In some cases chloroform excelled in mobilizing pesticides from soil, however mostly application of humic acid solution as extraction model was found to be at least as efficient as methanol, chloroform or CaCl2-solution.
Four chemically much different pesticide (simazine, acetochlor, chlorpyrifos and diuron) were applied to two soil types (both sandy and brown forest). The extracted amounts were determined by GC/MS technique. Adsorption coefficients (Kd) were also calculated for the examined samples.
Obtained results for Kd indicated that chemical feature of pesticides seemed to be of utmost importance in terms of soil binding capability preceding the relevance of soil characteristics. Adsorption capability of chlorpyrifos proved to be the most pronounced preceding simazine and the least prone to bind to soil acetochlor and diuron -
Changing of some parameters of the soil-plant system as an effect of different composts
262-266Views:80Composting is an alternative way for practicing site-specific and environmental friendly plant nutrient supply. Our aim was to study the effect of different composts on plant and soil system.
Pot experiment with acidic sandy soil blended with three composts in five (0%, 5%, 10%, 25% and 50%) proportion was set up. Our experimental plant was perennial ryegrass (Lolium perenne L.). After the harvest of ryegrass we measured the fresh and dry weight of harvested plants, the P-, K-, Mg-contents, and the pH of soils.
The three composts had different effect on dry weight production and on nutrient-supply of soil. We established that one of the compost had significantly larger effect on the dry weight of ryegrass compared to the others. In this study we proved that favourable compost/soil proportion is different in the case of different composts. -
The effect of biopreparations in pot experiment
45-49Views:129In pot experiment the effect of Amykor and Organic Green Gold bioproducts and their combinations with NPK fertilizer on some soil properties (chemical parameters) and on the biomass of testplant were studied. The experiment was set up in 2012 at the Institute of Agricultural Chemistry and Soil Science, in a three replications, in a random block design. The studied soil typein the pot experiment was humus sandy soil from Debrecen-Pallag with onion (Allium cepa) test plant. At the end of the experiment (after 4 week) in our laboratory the samples of soil and plant were determined. The nitrate-nitrogen, AL-soluble phosphorus and potassium content of soil, the weight of green onion leaves, the wet weight of bulb and root of onion and biomass of onion. The results of the study were the following: – The treatments influenced positively the nitrate-nitrogen, the AL-soluble phosphorus and potassium content of soil. – The most effective treatments were the artificial fertilization (NO3-N) and the NPK+ simple dose of Amykor (AL-P2O5 and Al-K2O). – The NPK fertilization and the NPK+OGG (sprinkle in every 10 days) combinations had significant positive effect on the weight of green onion leaves. – The biofertilization and the straw+biofertilizer combinations stimulated the AL-soluble potassium content of soil occasionally. – The OGG treatment (sprinkle in every 10 days) had significant effective impact on the wet weight of bulb and root of onion. – The biomass of onion was increased by the artificial fertilization and OGG (sprinkle in every 10 days) treatment.
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The study of the fertilizing effect of wheat straw ash in a greenhouse experiment
47-51Views:172The effect of wheat straw ash as a fertlizizer was studied in a pot experiment with an acidic sandy loam soil (pHKCl=4.9) with weak K and P supply. The test plant was ryegrass (Lolium perenne). The treatments were the following: 1. control untreated soil, 2. NPK fertilizer, 3. small dose of ash (1.4 g kg-1), 4 large dose of ash (2.8 g kg-1), 5. small dose ash completed with NP fertilizers. Soil parameters (pHH2O, pHKCl, ammoinum-lactate soluble P, K, 0.01 M CaCl2 soluble PO43--P, K, Mn, Cu, Zn ) and plant parameters (yield, P, K, Ca, Mg, Zn, Mn uptake) were investigated. Based on the analysis of the straw ash sample and the results of pot experiment it can be stated that the wheat straw ash is suitable for the fertilization of the studied soil. The small dose ash completed with NP resulted in the largest yield increment (43%). In order of the treatments the pHKCl changes to: 4.9, 4.8, 5.2, 5.8, 5.1. As the N : P2O5 : K2O ratio is 0 : 1: 3.5 in the wheat straw ash sample, to reach optimal yield ash should be completed with N and P.
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Effect of agrotechnical factors on the yield of the Grolim asparagus (Asparagus officinalis L.) hybrid on acidic sandy soil
43-48Views:211Research of blanched asparagus has begun at the University of Debrecen Institutes for Agricultural Research and Educational Farm Research Institutes of Nyíregyháza in 2011. Establishment of the plantation took place in May 2011. The Grolim hybrid was used in the trial, 16 medium plot trial area has been formed under field conditions, with four repetitions and 36 m2 plot size. In the course of our studies, the effect of different nutriment supply methods (untreated, manure, sheep manure compost, fertilizer) has been analysed on the spear yield of the Grolim asparagus hybrid between 2013 and 2017.
In our studies, the beginning of vegetative growth has been recorded upon the constant presence of 10 °C of average soil temperature in the case of the Grolim asparagus hybrid. The beginning and length of spear harvesting are both influenced by the time and dynamics of initial development in spring. During the analysed period, the dates of spear harvesting were various, the earliest being on 23rd March, 2014 and the latest on 23rd April, 2015; the rest of the three years have been varied within this one month interval. The total of heat units required for the vegetative development of spears has been determined; it provides important information for cultivation practice.
Spear yields turned out to be hectic during the analysed period. In 2013 and 2014, yields have surpassed the amount of 50 kg/harvest period/plot in the case of every treatment version. However, in 2015 a significantly lower specific yield has been recorded due to the unfavourable weather conditions in spring; a yield decline of nearly 50% was recorded in the case of the control treatment compared to the previous years. Yield was also lower in the rest of the fertilization treatments compared to 2014; however, in these cases, the degree of yield decrease was around 5–10%, which suggests the yield stabilising effect of fertilization. In 2016, a slight yield increase was measured in comparison with the base year. In 2017, there was a decline of yield in the control treatment; however, the different fertilization treatments resulted in yield increase as compared to previous years.
On the basis of our studies, it is clear that the best yield results have been provided by the artificial fertilization treatment in all of the five analysed years. It was followed by the sheep manure compost and manure treatments in terms of their effect on spear yield. During the three harvesting periods, the lowest yield on acidic sandy soil was recorded in the case of the control treatment. The most remarkable effect of nutriment treatments has been realised in terms of the decreased deviation of yield results, which perfectly represents the yield stabilising effect of nutriment supply in the case of perennial crops – asparagus – as well, even on a poor nutriment supply characteristic sandy soil.
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The effect of compost application on physical properties of sandy soil
67-70Views:150The sewage sludge compost is suitable to improve the colloid-poor sandy soils, which are common characteristics of poor water- and nutrientholding capacity. The general characteristics of sandy soils are the light mechanical composition, the low content of humus and mineral colloids, large pore size and a bad aggregate stability. They have a poor nutrient supply capacity, due to its high porosity the organic matter is degraded very quickly to mineral colloids (Stefanovits et al., 1999).
By the compost application the soil is enriched mineral and organic colloids, thereby improving the soil structure. The effect of addition of compost to soil the water- and nutrient-holding capacity and porosity could be increased and the bulk density could be decreased (Martens and Frankenberger, 1992).
The aim of our experiment is to carry out physical measurements to determine the effects of compost treatment. In this study the results of the first year are presented.
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Investigation of Chromium(III)-Picolinate Adsorption on Some Soil Types
190-193Views:57In the experiment adsorption characteristics of different soil types (humic sand, meadow soil, leached chernozem and meadow solonec) was examined on the basis of adsorption isotherms for Cr(III)-picolinate. The Langmuir equation was used to describe the isotherms by which the amounts of metal ions actually and maximally adsorbed by the soils were determined concerning the given complex. A comparison was made among the organically bound Cr(III)-picolinate, an inorganic Cr(III) compound and a Cr(VI) form examined in a previous study. Based on the adsorption isotherms, adsorption capacity of the Cr(III)-picolinate was found 20 times smaller on sandy soil and 50 times smaller on the chernozem comparing to that of the inorganic Cr(III)-chloride, thus, the bio-availability of the chromium for the plants is 20 and 50 times higher in case of the given soil types. For the well-known toxic Cr(VI)-form, the adsorption was 2 times higher in case of sandy soil and 5 times higher for chernozem than in case of the organic Cr(III)-complex compound.
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Effect of fertilization on the potentially mineralize N forms of soil of long term field experiment was set in an acidic sandy soil
20-24Views:120The aim of this paper was to provide further information about the nitrogen mineralization processes of soil. A modified incubation technique was applied to establish the amount of easily soluble mineral and organic N forms during the incubation period. An acidic sandy soil was used for incubation, which was sampled from the „Westsik” long-term field experiment. The incubation was carried out on fifteen selected soil samples which were received different treatments since the experiment was set up.
From the obtained results, the amount of potentially mineralizable N and the mineralization rate constant were determined. Results of chemical analysis and biological interpretation of results are discussed. -
Untersuchungen zur Phosphataufnahme von Zwischenfrüchten bei unterschiedlicher P-Versorgung des Bodens (Investigation of P Uptake of Catch Crops under Different P Supply)
7-14Views:176The P uptakes of 11 different catch crops on four soils were estimated and compared with changes of double lactate soluble Phosphorus (P(DL)) in soil within a vessel trial. Additionally a model trial with quartz sand was carried out for investigations of the influence of P supply on root parameters. The differences of P uptake in dependence of the proofed variants were significant. Under a sufficient P supply Buckwheat, Maize and Oil radish had the highest P uptake on sandy soils, on loamy soil also Buckwheat and Maize but Serradella too. Under P deficiency the withdrawals of Phosphorus for Buckwheat, Maize and Oil radish were found to be the highest. In contrast to the sandy soils for the loamy soils no significant relation between the P uptake by plants and changes in the P-DL amount in soil could be found. For all variants the P uptake by plants were higher than the reduction of the P-DL amount in soil. The rate of P-DL content on the total P content in soil reduced while the two trial years only in sandy but not in loamy soils. The P uptake, the root length and the root/shoot relationship depends significantly on the cultivated crop and the added P compound. The added water soluble KH2PO4 caused a higher P uptake but a lower root/shoot-relationship than the water insoluble P compounds.
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The impact of various grape stock cultivars on the As, Cu, Co and Zn content of the grape berry (must, seed)
39-44Views:133Scientific research from the last decades showed that the inappropriate industrial and agricultural production caused an abnormal increase of the potentially toxic elements in the soil. Unfortunately the acidification of the soil is an increasing problem in Hungary. According to Várallyay et al. (2008) 13% of the Hungarian soils are highly acid. Accumulation of toxic elements differs in the genetically diverse plant species. The root of the plant constitutes a filter so that the rootstock is also kind of a filter system, which may prevent that the scion part (such as berry) accumulate high levels of various potentially toxic elements from the soil. The aim of research was to determine how different grape rootstocks influence the As, Co, Cu and Zn content of the musts and seeds. Thus, specifying which of the grape rootstocks takes up the lowest level of these 4 elements (As, Co, Cu and Zn), and accumulates in berries, so could reduce the potentially toxic element load of the grape berries. The grape rootstock collection of the University of Debrecen was set up in 2003 in 3x1 m spacing on immune sandy soil. Grafting of ‘Cserszegi fűszeres’ was started in 2010. We could evaluate yields harvested from 12 rootstock varieties of the experiment in October 2011. We obtained valuable differences in the arsenic, copper, cobalt and zinc concentrations of musts and seeds of ‘Cserszegi fűszeres’ grafted into different rootstocks. The results obtained from the 2011 harvest support the statement that the choice of rootstock might be an important factor to increase food safety. The differences in concentration of the four elements observed in case of the rootstock may have been caused on one hand by the rootstock effect, and on the other hand, the vintage effect has a very significant impact on the vines element uptake. Several years of experimental results will be needed to answer these questions.
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Economic questions of maize production on different soil types
289-292Views:103The requirements and objective of cultivation are in constant change. For example, different cultivation systems are developed for the purpose of soil protection, the preservation of its moisture content and on soils with various precipitation supply or production site conditions. Traditionally, one of the most important cultivation aims is crop needs. Further cost saving in fertilisation and crop protection can only be achieved by reducing the quality and quantity of production or it cannot be achieved at all. Furthermore, the costs can be significantly reduced by means of the rationalisation of cultivation. Energy and working time demand can also be notably reduced if ploughing is left out from the conventional tillage method. The key requirement of economicalness is to perform the cultivation at the optimal date, moisture level and the lowest possible cost.
Within production costs, the cost of cultivation is between 3–17%, while they are between 8–36% within machinery costs. It is the vital condition the usability of each technological method to progressively reduce costs. Our evaluation work was carried out with the consideration of the yield data obtained from cooperating farms and the experiment database of the Institute for Land Utilisation, Regional Development and Technology of the Centre for Agricultural and Applied Economic Sciences of the University of Debrecen. Three technological methods (ploughing, heavy cultivator and loosening tillage) were used on several soil types which differ from in terms of cultivability (chernozem, sandy and sandy clay soils) from the economic/economical aspect. We examined the sectoral cost/income relation of maize production as an indicator plant. The maize price during the analytical period was 45 thousand HUF per t. On chernozem soils, the production of maize can be carried out on high income level, while maize production on sandy soils has a huge risk factor. The role of cultivation is the highest on high plasicity soils, since they have a huge energy
demand and the there is a short amount of time available for each procedure in most cases.