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• Application of laparoscopic artificial insemination in conventional Lacaune sheep farm using frozen-thawed semen

  133-138

  Zemenu Birhan Zegeye

  Nóra Vass

  Andualem Tomano

  Views:

  237

  The complex anatomical structure of the ewe reproductive tract accompanied with low quality of frozen ram semen for artificial insemination, resulted in a challenge with regard to using superior genotypes for reproductive ovine performance. Hence, improved genetics in ovine management has not been efficiently and widely used especially in undeveloped countries. Therefore, intrauterine semen deposition by laparoscopic insemination should be adopted in the current sheep production systems. Thus, this study aimed to assess the pregnancy rate and lambing rate of ewe inseminated by laparoscopic insemination techniques using frozen-thawed semen. The research used imported frozen semen from two rams of the Lacaune breed. Ewes were grouped according to age in years (1, 2 and 4). Before insemination, the semen was examined microscopically for its motility and viability and thereafter the laparoscopic artificial insemination technique was performed to 19 Lacaune breed ewes using frozen-thawed semen. The overall pregnancy and prolificacy rates were 31.57% and 42.10% respectively. Out of 2 ewes in the 1-year age group that were inseminated, only 1 ewe lambed representing 50%. However, from 16 ewes inseminated of four-year age group, 5 ewes lambed representing 31.25%. Significant difference based on age group was not evaluated due disproportionate of the data, (such that the data included 2 ewes in one-year-old age, 1 ewe in 2-year-old age and 16 ewes in 4-year-old age). Based on the ram semen, 33.33% and 30% of the inseminated ewes were pregnant from ram A and ram B semen respectively. However, in the case of prolificacy rate, 44.44% and 40 % of the ewes lambed from using semen of ram A and B, respectively. There was no significant difference (p>0.05) in pregnancy and prolificacy rates based on semen from the two rams. In conclusion, in this research study, ram semen had no significant effect on pregnancy and prolificacy rates using laparoscopic AI on Lacaune sheep. This could be due to the fact that the rams had very good quality semen. Evaluation of ram semen, accompanied with appropriate ewe selection based on age and rightful deposition of semen could lead to better and more consistent results. Overall this could contribute to the successful application of laparoscopic artificial insemination in Lacaune sheep production systems for enhanced productivity.

• Development of the reproductive tract of immature gilts

  165-166

  Anna Zmudzińska

  Wojciech Kapelański

  Views:

  59

  Mechanisms of regulation of litter size in pigs are complex and depend on many factors, including genetic regulation and also physiological and anatomical development of the reproductive tract in gilts. Improvement of growth rate in present breed pigs raises the question as to whether, with the development of the reproductive tract, sexual maturity would also be attained.
  The aim of the study was to assess the morphometric traits of reproductive tracts taken from gilts slaughtered at 100 kg body weight, i. e., just when they may become actively sexual mature.
  This study was concluded on 80 prepubertal gilts of the Polish Landrace (PL) breed tested at the Pig Program Testing Station. The animals were kept in individual pens with control feeding and standard management. They were slaughtered after attaining 100 kg body weight. Immediately after slaughter, the reproductive tract was removed and carefully assessed. The morphometric estimation of the reproductive tract involved the
  measurement of uterus weight with ligament, vagina-cervix length, uterine horns and oviducts length, ovaries weight, height and width. Uterus volume capacity was also determined, based on volumetric method of Kwaśnicki’s (1951) with own modification.
  All pigs were divided into three groups in respect to age at slaughter: A – below 160 days (n=38), B – from 160 to 180 days (n=28) and C – above 180 days of age (n=14). The results were elaborated statistically computing the arithmetic means (x) for every traits and standard deviations (s). One-way analysis of variance ANOVA was performed. The significance of differences between age groups was estimated using Duncan’s test.
  Calculations were performed with STATISTICA 8PL Software.
  Obtained results are presented in the tables below. The most pronounced differences in the development of the reproductive tract are dependent on the age of gilts concerning only the uterus weight (P≤0.01) and uterus vagina-cervix length (P≤0.05). Gilts at age 160-180 days attained the full stage of reproductive tract development. Differences between the compared age groups of gilts dealing with the other morphometric traits
  and ovary characteristics were statistically not significant.