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  • Effects of bioactive plant extracts on immune-related gene expression of common carp (Cyprinus carpio)
    49-56
    Views:
    241

     In recent years, intensive fish farming has led to an outbreak of several diseases, and the health status of fish can affect the economy of aquaculture. Since fish health and intestinal health are in correlation, it may also have an impact on immunity. Accordingly, many natural feed additives are being used to improve immune functions. In our study, carotenoids, oligosaccharides, and anthocyanins were applied at 1 m/m% in feed to investigate their effects on cytokines, such as interleukin-1β (IL-1β), interleukin-8 (IL-8), tumor necrosis factor-α (TNF-α), and interferon regulatory factor-1 (IRF-1) in spleen and mid-intestine of 6 months old carp. Gene expression analysis was carried out to examine IL-1β, IL-8, TNF-α, and IRF-1 mRNA levels in fish spleen and mid-intestine. The gene expression level of pro-inflammatory IL-1β decreased in the mid-intestine of carotenoid-fed carp compared to anthocyanin supplemented group, but the effects of the bioactive plant extracts were not observed on the examined cytokines compared to control fish.

  • Harmonic development and biodiesel
    91-95
    Views:
    76

    An increased expansion of renewable resources and biodiesel is observed and prognosed, since fossile energy resources are about to run out. Hungary achieved outstanding sunflower years in the recent years in worldwide comparison. In the future, the feedstock of biodiesel production can also be rape besides sunflower.
    According to the concept of harmonious development, the balance between nature, society, economy and human environments is represented by their mutual presumptive character. Research and development need to be aligned into this system. Our aim was to examine the advantages and
    disadvantages of biodiesel production in different environments, using a model to do so. In order to maintain the harmony, the existing resources have to be managed properly, taking the correlations of the system into consideration. Targeted technological developments are necessary, similarly to the improvement of energy safety and efficiency.

  • The significance of local meteorological stations in research planning
    87-91
    Views:
    184

    The goal of research planning is to optimize human and material resources while maximizing efficiency. If there are databases that can be used as a substitute for own data collection, effective research can be facilitated by reallocating resources. In the case of environmental studies, the knowledge of the climatic conditions of the study period is known to be a key factor in research planning process.

     In the present study, the data of our meteorological station in our research area (known as “Szamárháti Tanya”, Kesznyéten, Hungary) was compared with the measurements of meteorological stations operated by the competent water authority. Stations were taken into the study within a 10 and 20 km radius over a period of 21 months, to determine which provided more relevant data from the area.

    During the evaluation of results, the relationship between the local and regional weather stations were determined, and deductions were made from the obtained results to support the decision which of the targeted investigations could be of greater benefit. 

  • The effect of β-glucan, carotenoids, oligosaccharides and anthocyanins on bacteria groups of excreta in broiler chickens
    15-20
    Views:
    168

    This study was conducted to examine the effect of natural compounds, such as β-glucan, carotenoids, oligosaccharides, and anthocyanins in the diet on bacteria gropus of excreta in Ross 308 broiler chickens. Chickens were fed 5 diets: control (basal) diet, a diet supplemented by β-glucan at 0.05%, and diets supplemented by carotenoids, oligosaccharides, or anthocyanins at 0.5% of each compound. On experimental day 19, excreta were collected to determine the proportion of Lactobacillus, Bifidobacterium, Campylobacter, Clostridium, and Escherichia coli. Samples were collected aseptically and snap-frozen in liquid nitrogen. Bacterial DNA was isolated from samples, then polymerase chain reaction using primer pairs designed to the 16S rDNA of bacterial groups were applied to define the proportion of the mentioned bacteria. Another universal primer pair was used to amplify a region of 16S rDNA of all the examined bacteria. Proportion of each bacterial groups was determined relatively to the intensity of universal PCR product band by gel documenting system and ImageLab software. Based on the results, carotenoids and anthocyanins increased the proportion of Bifidobacterium, which might imply the beneficial effects of the mentioned compounds on the bacteria composition of excreta.

  • Examination of requirements of labour market, connected with competencies of agrienvironmental engineers and nature protection engineers
    81-87
    Views:
    97

    The most important goal of firms is to supply demand of their economic partners. To make it successfully, highly qualified human resource is needed. The quality of human resource is determined not only by qualification, command of a language, professional experience, practise, but extant competencies that can also be developped. To choose the expectant employee, it is not enough to have intelligence and proficiency. It is also decesive to examine particular competencies. All of these will define the employee’s achievement that can be the right way to the success of
    the enterprise. 
    It is need to make the cooperation between higher education institutions and employers closer. As a result of this, employers can know theirs ways about possibilities of the new higher educational training system. They can use professional knowledge, competencies of the Bologna system’s graduates at different pointsof the economic life. The higher education institutions can revise their training systems considering requirements of labour market. According to these requirements, they can form syllabus in order to train auspicious experts.

  • Effect of allithiamine on the level of hyperglycaemia-induced advanced glycation end products
    41-44
    Views:
    226

    Diabetes mellitus is a rapidly growing public health burden in both developed and developing countries. Diabetes mellitus is accompanied by hyperglycaemia, which can cause tissue injury by several mechanisms. One of these is the formation of advanced glycation end-products (AGEs). In this study, the effect of allithaimine, a fat-soluble thiamine derivative, was investigated on hyperglycaemia-induced AGEs levels using human umbilical cord vein endothelial cells (HUVECs) as a hyperglycaemic model. HUVECs were isolated by enzymatic digestion, characterized by flow cytometer and treated 30 mM glucose plus allithaimine or thiamine or cell maintenance medium as control.  Allithiamine was synthesized and purified. The structure of the synthesized and isolated compound was verified by reverse phase HPLC and MALDI-TOF. AGEs were evaluated by ELISA. Collectively, our results indicate that allithiamine can reduce level of the hyperglycaemia-induced AGEs similar to thiamine.

  • Daily soil carbon dioxide flux under different tillage conditions
    141-144
    Views:
    156

    Over the last few years, warming of the atmospheric layer near Earth's surface is increasingly experienced and researchers have also established that concentration of numerous greenhouse gases have risen over the past two centuries value. Change is basically a legitimate process - considering atmospheric concentration as well - but the change experienced during the past centuries could not have become this critical without the contribution of human activity. Due to the nature of the greenhouse effect, the result of a very fragile, complex process is experienced currently on Earth, which can be significantly unbalanced even by a slight change. Carbon dioxide emitted from the soil is involved in the global cycle and has an impact on the greenhouse effect. The rise in soil respiration may result in the further intensification of warming. In the scope of the present study, it was examined how carbon dioxide emissions of the soil evolve over a day. The results have been established based on the comparison of the effects of different parts of the day, tillage methods and irrigation.

  • Effect of anthocyanin-rich sour cherry extract on the level of IL-8 in LPS-induced endothelial cell
    27-30
    Views:
    196

    The anthocyanin content of the Hungarian sour cherry is remarkable. Nutraceutical and pharmaceutical effects of the anthocyanins and their role in disease prevention have been studied extensively. Endothelial cells are involved in the pathogenesis of several inflammatory diseases. The objective of this work was to investigate pure sour cherry extract on human umbilical cord vein endothelial cells (HUVECs) as an inflammatory model.  HUVECs were treated with 100 ng/mL lipopolysaccharide (LPS) and 50 mg/mL sour cherry extract or M199 medium as control. The optimal concentration range of the sour cherry extract was investigated and selected based on MTT assay measuring the conversion of the tetrazolium salt to formazan by mitochondrial dehydrogenases. The level of interleukine-8 (IL-8), a pro-inflammatory cytokine, was measured in Luminex MagPlex assay. LPS treatment significantly increased the secretion of IL-8. The pure sour cherry extract was able to attenuate this increment indicating the potent anti-inflammatory effect of pure sour cherry extract. Our results emphasize that pure sour cherry extract could reduce the LPS-induced inflammatory response thereby may improve endothelial dysfunction.

  • The importance of predator species in the population dynamics of the Brown hares (Lepus europaeus, Pallas 1778) – Literature review
    43-49
    Views:
    173

    One of the conditions for successful small game management is the good management of predator species. The predator species play an important role in the sustainable utilization of the domestic brown hare populations. A portion of these species are under nature protection and with the rest of the species can be utilizing by the wildlife management professionals. Important prey species of brown hares: perspective are red fox, domestic dog and domestic cat. Based on latest date of the National Game Management Database in hunting bags increasing every year the number of the European badger, the stone marten and the golden jackal. In Hungary the brown hare’s most important predator bird species are common buzzard, marsh-harries and goshawk. The human race is not only as a top predator affects the number of the population of brown hares with the wildlife management but indirectly with traffic, (soil cultivation, mowing, and pest control) as well. The control of predators is absolutely necessary for successful small game management, but without sufficient habitat size and habitat development it is hardly sufficient.