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Evaluation of dry matter accumulation of maize (Zea mays L.) hybrids
35-41Views:336The increase of the grain yield of maize is closely correlated with its seasonal dry matter accumulation. Dry matter is accumulated into the grain yield during the grain filling period. The following maize hybrids were involved in the experiment: Armagnac FAO 490, Loupiac FAO 380 and Sushi FAO 340. In order to determine dry matter content, two samples per week were taken on the following days: 22nd, 25th, 28th, 31st August, 4th, 7th, 14th, 18th, 22nd, 25th, 29th September and 2nd, 6th, 9th, 13th October. In the course of sampling the weight of 100 grains from the middle section of 4 ears was measured in 4 replications. Dry matter content was determined after drying to constant weight in a drying cabinet at 60 °C. Harvesting was performed on 13th October 2017.
The daily precipitation sum was determined by local measurements, while the daily radiation and temperature data were provided by the Meteorological Observatory Debrecen of the National Meteorological Service in Budapest. Among the agrometeorological parameters, an analysis was made of the precipitation during the growing season, effective heat sums during the vegetative and generative phase, and the water supplies. The daily heat sums were determined using the algorithm proposed.
The amount of precipitation in the winter period before the 2017 growing season was 210 mm. The soil was saturated until its field capacity. The rather dry and warm March and April had a favourable effect, but there was no worthy amount of precipitation until May (51 mm) due to the condition of the dried seedbed. Sowing was performed on the 5th of May 2017 in a randomised small plot experiment. There was favourable precipitation and temperature during the growing season, thereby providing ideal conditions for maize development, growth and yield formation. There was near average amount of precipitation in each year. The total amount of precipitation in the summer period is 342 mm. Temperature was mostly above the average, but there was no long and extremely warm period.
The Armagnac hybrid reached its highest dry matter mass 126 days after emergence. Physiological maturity was reached sooner (on the 119th day) in the case of Loupiac, and even sooner in the case of Sushi (116th day). The thousand grain weight of Sushi (which has the shortest ripening period) was 286 g at the time of physiological maturity, while that of Loupiac was 311 g. Compared to Sushi, Armagnac showed 12 g more dry matter accumulation (306 g). In the case of all three examined hybrids, physiological maturity was preceded by an intensive phase, when the dynamics of dry matter accumulation was rather quick. On average, Sushi gained 2.8 g dry matter per day between 103 days following emergence and physiological maturity, while the same values were 3.2 g for Armagnac and 3.3 g for Loupiac. The aim of the regression line slope is to predict the behavior of the dependent variable with the knowledge of the values and characteristics of the independent variables using the regression line equation. Furthermore, to determine how the location affected the dynamic of dry matter accumulation in the Armagnac, Loupiac and Sushi hybrids. In regression analysis, the coefficient of explanation showed that the effect of day in the Armagnac was 97%, in the Loupiac 94%, in the Sushi 90 %. The determination coefficient (R2) is useful in determing how the regression equation fits. But, as we have seen, the determination coefficient alone is not sufficient to verify the model’s accuracy, in addition to the determination coefficient (R2), the normality of the data or the residuals, the variance of the variables at different levels, the independence of the data relative to time and non-oblique. Observations are evaluated for the correctness of the fitted model.
Dry matter values decreased evenly and slightly following physiological maturity. According to our research results, it was established that physiological maturity is followed by a moderate dry matter loss. Until harvesting, Armagnac lost 40 g of its thousand mass weight in 29 days, while the same value pairs were 69 g in 36 days for Loupiac and 29 g in 39 days for Sushi. Loupiac – which had the highest weight at the time of physiological maturity – lost the most of its dry weight; therefore, Armagnac and Sushi had higher values at the time of harvesting.
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The effect of lead and copper heavy metal salts on soil microorganisms under laboratory circumstances
55-59Views:137he population dynamics of calcareous chernozem soils polluted with different concentrations of lead and copper heavy metal saline solutions was examined.
The experiment was carried out in the soil biological laboratory of the Institute of Agricultiral Chemistry and Soil Science at DE AGTC MÉK in 2012. For the determination of the concentration of the undiluted stock solutions we multiplied the smallest toxic concentration values of the MSZ 08-1721/1-86 Hungarian standard by forty. The intermediary concentrations of the treatments were produced with adequate dilution of the stock solutions until a dilution level equal to the values of the standard. The statistical evaluation of the data was performed with ANOVA (Analysis of Variance) including the determination of the standard deviation and significant difference. Investigating the effects of the different treatments on the soil microbes we established that both heavy metal saline solutions had a negative effect on the population dynamics of bacteria and microscopic fungi living in the soils. The negative effect of copper – as a potential toxic micro nutrient – turned out to be less strong than the negative effect of the toxic lead. According to our results the correction of the treatment levels is recommended in order to further tolerance examinations and the determination of the tolerance levels.
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Decreasing Energy and Water Use in the Meat Industry
24-28Views:61The technology supervisions are needed from time to time in order to involve the new research results, experience, new technologies and equipments organising principles into the production and to make it cheaper and with less expenditure to increase the profitability and competitiveness. In the course of our investigation we aimed the analysis of the traditional technologies and improving of it. In this article we detail the heat treatment curing process. We applied the principles of experimental design for the determination the main influencing parameters in the processes. Then we made simulations and modelling in seeking for the environmentally better technology programs. We developed a method for measuring the temperature development of products with small diameter using the similarity theory in unit operation. In the course of the analysis of the heat treatment processes we could state that the heat treatment time can be reduced significantly by decreasing the size of the product and choosing the right ratios among the surface heat transfer intensity, ambient temperature and involving the lethality obtained during the cooling phase. The total sparing were about 10-20%. We solved the problem associated with the sensor placement error using a plastic material (metamid). We establish a curve between the real product temperature and plastic rod temperature by which the monitoring of the process became more accurate. We determine the size modification for different product sizes. In case of curing we observed very similar phenomena. For the determination of the diffusion coefficient we adopted the Ball-method not using in this field till today. We could reduce the excess saltiness and loss of NaCl with about 5%.
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Managing risk using real options in company’s valuation
125-132Views:133The valuation of company is very important because provides information about the current value/situation of company, and through this, provide the opportunity of choosing the best company’s growth alternatives. The future strategic decisions are characterized by lack of knowledge, information, so all measures of company’s growth are closely linked with uncertainty and risk. The company’s valuation process is also related with uncertainty and risk. The risk may result both from the assessed assets and the technique used. In literature, we could find three approaches for risk management: capital budgeting based method, methods based on portfolio analysis and real options approach of risk management. Among them, the real options based methods is the most revolutionary approach for risk management. The advantages of the method, consists in the fact, that the process of establishing strategic decisions integrates the possibility of reversibility, delay and rejections, which isn’t it possible at two previous methods. The method also takes into account the total risk of company, so both the company-specific and systematic risk. In this study, I have used one of the best-known real option based method, the Black-Scholes model, for determining the option’s value. Determination of option value is based on the data of enterprise, which was tested Monte Carlo simulation. One of the basic assumptions of the Black-Scholes model is that the value of option is influenced by several factors. The sensitivity of option’s value could be carried out with so-called “Greeks”.. In the study the sensitivity analysis, was carried out with indicators Delta (Δ), Gamma (Γ) and Vega (ν). The real options based risk management determinations were performed in the R-statistics software system, and the used modules are 'fPortofio' and 'mc2d'. By using of real options method, I have calculated the average value of company capital equal with 38.79 million. By using simulation was carried out 1000 runs. The results of this show a relatively low standard deviation, small interquartile range and normal distribution. In the calculation of indicator Delta, could be concluded the value of company moves in 0.831 proportion to the price of options, the standard deviations of index is low, so the real option based method could be used with success in company’s value estimation. The Gamma index shows the enterprise value is sensitive just for large changes. The result of Vega reflects the value of option, so the company’s value volatility, which is small in this case, but this means a volatility of value. In summary, we can conclude that the call options pricing model, well suited for the determination of company’s value.
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Supplementary botanical examinations for modelling the grass production of the great pasture of Hajdúbagos
17-21Views:59Our botanical survey at the great pasture of Hajdúbagos is a part of a broad research that aims to predict the production of the grass at the given area. As the mentioned pasture is a nature conservation area, the usage of artificial fertilizers or other classic grassland management methods in its handling are prohibited. Thus grazing is an important tool for the management of this area, however the not suitably regulated grazing order and the poorly calculated carrying capacity cause serious problems at some parts of the pasture. The prediction of the grass yield is essential to
avoid both over- and both under-grazing and for determining the optimal number of the grazing animal stock and the grazing method, thus the most suitable management strategy.
The potential grass yield is easily calculable with a computer model that will be established as a basis for determining the grass production. For the sake of getting an accurate view of the plant associations of the pasture, we created examination quadrates and determined all plant species found in the quadrates. After plant determination, we compiled a coenological table in which we marked besides the scientific name and families, the life forms of each species that refer to the structure, morphology and thus the adaptability of plants to their environment. We determined the
TWR, so the thermoclimate, water and soil reaction values, the nature conservation values, as well as the covering values of each plant species (DB), and the total coverage of the examination quadrates (B%).
According to the covering values, grasses proved to be characteristic plants at the examined pasture, thus we need to consider them influential in calculating the animal carrying capacity and with the rest of the information, we need to supply the model.
The life forms and TWR indicators, all together with the nature conservation values provide further important data to the development of the management suggestion of the protected pasture. By examining these values to different parts of the area, we could get an exact view on the measure of the degradation effects. This promotes the determination of grazing methods and the forming of the boundaries of certain pasture sections, to avoid those harmful anthropogenic effects that seriously endanger this extensive sandy pasture. -
The influence of channel network silting up at Žitný Ostrov to range of interaction between surface and groundwater
23-31Views:94The movement of water resources, especially the possibilities of their regulation by interaction between surface and groundwaters are the subject matter of attention particularly during the occurrence of extreme hydrologic situation. This work presents the overview of knowledge and results which were achieved at IH SAS in this question. It can show the ways how to optimize the adjudicated processes which emerge during the requirement of emergency intervention. The solution of this task was located at the Žitný Ostrov area because this territory with their existence of channel network is suitable for studying the surface and groundwater interaction. The channel network at Žitný Ostrov was built up for drainage and also to safeguard irrigation water. The water level in the whole channel network system has an effect on groundwater level on the Žitný Ostrov and vice versa. It was been necessary to judge the impact of the channel network silting up by bed silts on the interaction between channel network and groundwater on the Žitný Ostrov. The aim was to evaluate the changes of bed silt state of Žitný Ostrov channel network and consecutively their influence on interaction processes between groundwater and surface water along the channels in the period from 1993 to present. The measurements of bed silt thickness in Žitný ostrov channel network had been started from1993, later they continued at selected profiles of three main channels – channel Gabčíkovo-Topoľníky, Chotárny channel and Komárňanský channel (for checking of the silting up variability). From 2008 the detailed field measurements of cross-section profiles aggradations along these selected three channels have been started. The objective of detailed field measurements was the determination of the silt permeability which is expressed by parameter of saturated hydraulic conductivity. This parameter was determined by two ways – as the saturated hydraulic conductivity obtained from disturbed samples of silt Kp and as the saturated hydraulic conductivity obtained from undisturbed samples of silt Kn. In the first case the granularity of silts was determined as a first step and then was computed their Kp from the empirical formulas according Bayer-Schweiger and Spacek. From undisturbed samples of silts which were extracted along the channels from top, middle and bottom layer of silts, were determined the values Kn by the laboratory falling head method. The valid values Kp on channel Gabčíkovo-Topoľníky ranged from
4,33 10-7 to 4,46.10-5 m s-1, on Chotárny channel from 5.98 10-5 to 2.14 10-6 m s-1 and on Komárňanský channel fluctuated from 1.93 10-6 – 6.09 10-5 m s-1. The valid values Kn on on channel Gabčíkovo-Topoľníky ranged from 5.21 10-8 – 4.18 10-3 m s-1 , on Chotárny channel ranged from 8.54 10-8 – 2.70 10-4 m s-1 and on Komárňanský channel fluctuated from 4.72 10-7 – 1.26 10-5 m s-1. The remarkable results were noticed by comparison of values of saturated hydraulic conductivity from disturbed and undisturbed samples Kp and Kn. On Chotárny channel the values of silt saturated hydraulic conductivity from undisturbed samples Kn approximately hundredfold decreased (from 10-6 to 10-8 m s-1). On Komárňanský channel the comparison of values Kp and Kn shown that the values Kn from undisturbed samples approximately tenfold descended against Kp.Simultaneously, the bed silts‘ impact on the groundwater recharge (saturated hydraulic conductivity of silt) was also examined. Determination of the total recharge amount was done by numerical simulation (model SKOKY) and by the so-called method of interaction formulas. These two approaches were applied at the Žitný Ostrov channel network. There were field measurements performed in monitored three main channels and adjacent to obtain correct input data. These characteristics were used for simulation and computation of total recharge along the channels. The total recharge amount was calculated for four alternatives of the surface water levels in the channel and the surroundings groundwater respectively. We chose four simplified variants with the same geological conditions in surroundings area of channels, only water levels of groundwater and in channels were modified. The results of the simulations seem to show greater impact of the silt in the case of outflow from the channels to the surroundings than the inflow into the channel from the surroundings.
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Correlations study between the selenium content of wheat grass (Triticum aestivum L.) and wheat seeds grown on different soil types
117-121Views:96In the course of the research we determined selenium and dry-matter content of 35 wheat grasses and 35 wheat seeds. The selenium content of the preparation plant samples was measured by spectrofluorimetric determination (ʎexcitation=380 nm, ʎemission=519 nm) of the resulted piaz-selenol complex. It was established that between the selenium content of the wheat grass and wheat seed the correlation coefficient was 0.36 at p=0.05 level which indicates a medium close correlation. Similarly, there was a medium close correlation between selenium content of the wheat grass calculated on dry-matter basis and total selenium content of the wheat, with a correlation coefficient of 0.40 at p=0.02 level.
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Application of the decision tree, as the weak point of the HACCP system development
63-70Views:74In this article we have compared the „decision tree” method –suitable for the CCP determination during the HACCP systemintroduction – with the objective measurement method. We have found out, that the CCP-s from the two method differ from each other. So, the empirical „decision tree” method is suitable for determination of CCP-s only limited, while the measurementbased method determines the CCP-s objectively. Only the right
CCP-determination can guarantee safe food. -
Determination of element contents of commercial and homemade jams
71-75Views:218Various products could be prepared by using fruits as raw materials. In Hungary, the most popular fruit products are spirit, jams, preserves, juices and dried fruits. Jams are made from different fruits, with different fruit content, technology and additives. They can be used for different products or they can be consumed by themselves. These products and their related production processes have great traditions in Hungary. In addition, they are widely used.The aim of our study was to determine the element content of jams and to compare these samples based on their micro- and macroelement content.In this study, 26 fruit jams with different botanical origins were examined. 10 samples were commercial, and 16 samples were homemade products. The determination of micro- and macroelements was carried out by using Inductively Coupled Plasma Mass Spectrometer and Inductively Coupled Plasma Optical Emission Spectrometer. Different element contents were measured [B (249.7), Ca (422.6), Fe (259.9), K (769.8), Mg (280.2), Na (589.5), P (185.9), S (182.0), Al (27), Mn (55), Cu (65), Zn (66), Sr (88), Ba (137)]. Based on element contents, the jams showed statistical differences. -
The possibility of use of the 0,01 M CaCl2 and Baker- Amacher extractants for the determination of plantavailable potassium
7-15Views:71The Hungarian fertilizing recommendation systems use AL soil test for the evaluation of potassium supply. The 0.01 M CaCl2 is a definitely milder extractant, it extracts the easily soluble and exchangeable potassium amount. Its European introduction was already taken into consideration in 1994. The research project on this topic is started in several european countries, also in Hungary at the Department of Agricultural Chemisty of Agricultural University of Debrecen. Another advantage this multielement method is that the different element-ratios can also be calculated.
The Baker-Amacher extractant’s principle is that it contains a known amount of K, P, Mg in the CaCl2 solution. During the soil extraction adsorption and desorption process take place, so the adsorption or desorption can be calculated from the original and the final concentrations.
In this paper we introduce the results of comparing analysis of the samples (n=630) from Soil Information and Monitoring System. Our aim was to measure the use of new extractants beside conventional extractant (AL) for the evaluation of K-supply would be reasonable.
It can be stated that there is a medium close relationship (r=0.75) between AL-K and 0.01 M CaCl2-K. My calculations confirmed the results of former examinations, and proved that the two extractants don’t extract and change the same rate of K-fractions. We found that regression between 0.01 M CaCl2 and AL depend on texture classes, pH classes, amount of lime, and organic matter content of soils.
Comparing the relations between AL and Baker-Amacher we find relatively loose correlation (r=0.45). We stated that there are K-fixing soils among soils considered to be well supplied with potassium by AL. This might be caused by the high amount of mineral clay and the quality of mineral clay. We stated that the dK averages show that the Hungarian nutrient-supply categories characterize generally well K-supplement of soil.
It can be stated that it would be necessary to use new extractants to specify evaluation of plant available K. We found that the 0.01 M CaCl2 and Baker-Amacher extractants could complete usefully the AL procedure and could help effective potassium fertilization. -
Changes in the Diaporthe helianthi Infection of Sunflower Hybrids Between 1998 and 2002
214-233Views:65Plant protection, and especially the efficiency of protection against mycosis, is a very important production technological element concerning sunflower. The efficiency of production can be increased on the basis of a thorough pathological survey and its results carried out in a wide variety of hybrids, as the features of hybrids, the reactions of genetic bases to pathogens can be found out under domestic conditions.
The tests were carried out at variety-test lots of OMMI for hybrids used for food or oil and other sunflower varieties admitted by the state at the Experimental Site DTTI Látókép, Centre of Agricultural Sciences, University of Debrecen between 1998 and 2002. The number of the tested hybrids was 49 in 1998, 45 in 1999, 49 in 2000, 55 in 2001, and 44 in 2002.
Due to the infection source of high amounts remained from 1997 and the favourable weather conditions for the pathogens, an infection of high degree was experienced in the tested stands. The lowest infection index in the hybrids with a very short vegetative period was experienced with Beni hybrids. Comparing to the average of the hybridgroup with short vegetative period, a low infection index characterised the Baleno, Trident, IBH-166, Hysun 321, Resia, Alexandra, Cergold and Pixel hybrids. In 1999, among hybrids with medium vegetative period, Zoltán, Zsuzsa and Util hybrids could be highlighted because of their low infection index. In 1998, among confentionary hybrids, Marica-2 hybrid had the most favourable index values (2,55).
During our trials, the experienced infection dynamics were compared in the event of hybrids with higher and lower susceptibility under different sowing technological elements. The results call for the fact that when a hybrid with higher susceptibility is produced, production technological elements, such as the time of sowing, influence considerably the damage caused by the pathogen, therefore it must not be ignored when its determination takes place. In the event of hybrids with lower susceptibility the change of the tested production technological elements within the biological optimum does not lead to the increased risk of the damage caused by Diaporthe helianthi. -
Comparison of the sample preparation methods worked out for the examination of the element content of wine
77-82Views:113The examination of the potentially toxic elements content of the wines is not easy task, because the most elements are in little concentration (mg kg-1 or μg kg-1) in the wine and the wines contain great amount of organic matrix. The efficient sample preparation is essential for the accurate determination of element content. The eim of our research was to determine which sample preparation method will be the most efficient in examination of wines with ICP technology. The examined wine sample was a 2008 Chardonnay from the Eger wine region. We did the sample preparation and analysis examination in University of Debrecen, Centre for Agricultural and Applied Economic Sciences, Institute of Food Science, Quality Assurance and Microbiology.
We did the analysis examinations with ICP- MS (inductively coupled plasma mass spectroscopy). We always did the sample preparations and the examinations in three times rehearsal. The applied sample preparation methods: dilution with distilled water, open digestion and microwave digestion.
We were able to measure B, Al, Mn, Fe and Zn with only dilution and open sample preparation. In the smaller quantity present Sr and Ba were measurable in the wine in the case of all three methods well. We were able to measure the Co with dilution and open digestion method, while Cr, Ni, and Te with only dilution method. In the case of arsenic we were not able to measure reliable result with dilution and open digestion method because of organic matrix and other components
(alcohols, monosaccharides, polysaccharides, polyalcohols and inorganic salts). On the whole we are able to say that in the case of certain elements (B, Mn, Fe, Zn, Sr, Ba) the open digestion and dilution sample preparation is applicable well, however, in the case of certain elements (As, Al, V, Cr, Se, Mo, Cd, Hg, Pb) we have to develop the methods. It may be development of one of the way, if we develop sample preparation methods to examined element specifically and not
to wine generally. -
The effect of plant density on maize yield in average and extremely dry years
7-16Views:85The yield safety of maize has not been satisfactory in Hungary for decades. Yield is influenced by the combination of several factors.
In recent years, the frequency of dry years increased and fertilization decreased. These factors call for a rational determination of the plant density.
I studied the relationship between plant density and yield in 2003-2004 and 2007 on meadow soil.
In 2003, the weather was dry. In the vegetation period, the amount of precipitation was 78.5 mm lower and the temperature was 0.97 °C higher than the average of 30 years, the number of hot days was 47-60 (days with a temperature higher than 30 °C). However, we obtained favourable results under experimental conditions in 2003 after wheat as a forecrop using the fertilizer Kemira Power.
The weather in 2004 was favourable. In the vegetation period, the amount of precipitation was 93.2 mm higher than the average of 30 years. Although, the distribution of the precipitation could have been more favourable. The yield of the hybrids ranged between 8.87-10.42 t/ha. Among the studied seven hybrids, the early hybrids gave the highest yield at the highest plant density of 90 thousand plants/ha (PR38Y09, PR38A67, PR37D25, PR37M34). However, FAO 400-500 hybrids gave favourable results also at the low plant density of 45 thousand plants/ha (8-9 t/ha). At this plant density, the aeration of the plant stock was better and the hybrids were prone to bringing several cobs. Yield stagnated with increasing plant density (60 thousand plants/ha), then at 75-90 thousand plants per ha, the yield started to increase again.
In 2004 the yield of hybrids was considerably higher than in the previous year. In contrast to yields of 8.87-10.42 t/ha in 2003, yields in 2004 were around 9-12 t/ha.
The yield of the hybrid XO 902 P is above 12 t/ha already at a plant density of 45 thousand plants/ha. It gives maximum yield at the plant density of 90 thousand plants/ha.
The hybrid PR38P92 showed a good response to changing plant density, but its yield was only 9 t/ha at the low plant density value.
In a favourable year, the yield of the hybrids PR38B85, PR37W05, PR37D25, PR37K85 at a plant density of 45 thousand plants/ha 11 t/ha, while at the higher plant density of 90 thousand plants/ha, it ranges around 13-15 t/ha.Hybrids PR36K20, PR35Y54, PR34H31 have a good individual yield and they are prone to bringing several cobs in favourable years at a low plant density. Their maximum yield at the plant density of 90 thousand plants/ha is almost 16 t/ha.
In 2007, the weather was similar to that of the extremely dry year of 2003. The amount of precipitation in the vegetation period was 41.9 mm lower than the average of 30 years and its distribution was not favourable either.
In the optimum NPK fertilizer treatment at an optimum plant density, the yield of hybrids ranged between 9.32-10.73 t/ha. The highest yields of 10.22-10.73 t/ha were measured for hybrids PR38A79 (FAO 300) and PR35F73 at a relatively low plant density of 60 thousand plants/ha.
In the average of the hybrids, the optimum NPK dosage was N 131, P2O5 82, K2O 93 kg/ha active ingredient. -
Application of molecular genetic methods for birds (literature review)
37-41Views:119Ornithology studies have been extended by molecular genetic techniques. In this paper we are dealing with the most common use of areas. Our basic aim is to give a comprehensive view of the most commonly used methods in ornithological studies, including the available results by their use. We also deal with the following areas: an essential step during examination namely the applicability of sample collecting methods, and the unique identification, also the sex determination, methodology of DNA barcoding, as well as the role of molecular methods to protect endangered species. We discussed the advantages and disadvantages of the methods, such as the current trend for each method.
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Effect of net shading technology on the yield quality and quantity of chilli pepper under greenhouse cultivation
5-9Views:226This study was undertaken to identify the effect of net shading technology on the total capsaicinoids, vitamin C and relative chlorophyll content expressed as SPAD (Soil Plant Analysis Development) values and total marketable fruit yield of ‘Star Flame’ chilli pepper (Capsicum annuum) for two harvesting times cultivated under modified atmosphere. ‘Star Flame’ pepper was grown under three (3) different net shading colours (white, red and green). Samples without net shading were used as control. Samples were subjected to chromatographic analyses using HPLC for the determination of total capsaicinoids and vitamin C. Interaction in shading technology and harvest times (p=0.010) had significant effect on total capsaicinoids as a result of green and white shading technologies showing higher differences when compared to control samples. Vitamin C content was observed to have increased in white and red shadings (p=0.001) after the first harvest and gradually decreased after the second harvesting time (p=0.002). Relative leaf chlorophyll content was significantly higher in white shading in the first and second harvest. Samples used as control had significantly higher marketable values when compared to white and green shadings with red recording low marketability of ‘Star Flame’ chilli peppers.
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Determination of conversion rate values to characterise the ethanol production of the Kluyveromyces marxianus CBS712 fungus branch
23-27Views:69The ethanol production of the Kluyveromyces marxianus CBS712 strain was investigated among different kind of condition. We defined the conversion rate in order to know the efficiency of the ethanol production. To determine this value, it is crucial to characterize the residual glucose concentracio. We chose two method to determine the amount of residual glucose. The first method is the thin layer chromatography (TLC) and the second method is the method of the glucose-oxidase enzyme. We found that the TLC method is reliable than the other method. The conversion rates were determined from these values and the ethanol values. The maximal ethanol production of the characterized Kluyveromyces marxianus CBS712 strain (5.6% (v/v) ethanol at 45 °C, 55.3% conversion rate) is comparable to those strains which are applied in industrial ethanol production nowadays.
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Evaluation of the correlations between the normalized difference vegetation index (NDVI) and yield in a seasoning paprika (Capsicum annuum L. var. longum) stand
45-49Views:242The aim of our examination was to evaluate the correlations between the normalized difference vegetation index (NDVI) and yield, as well as to examine the possibility of yield estimation basedon NDVI in a seasoning paprika population.
Significant correlations were observed during the examination of the correlation between NDVI and yield. Furthermore, it was concluded that yield can be estimated with a 6.6–8.3% mean error based on the regression equations. No significant difference was shown between the error of estimations performed with various regression types and that of the estimations performed at various dates. For this reason, the identification of the optimum estimation method and the determination of the optimum date for estimation call for further examinations.
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Influence of phytophagous mammals environment-forming activity on the soil invertase fermentative activity in conditions of mining impact region
127-130Views:97Excretorial and fossorial activity of mammals is an important part of environment-forming activity. Mammals have influences on important biogeocenotic processes, especially on the soil processes. Determination the maintenance of soil invertase as one of diagnostic description the ecological state allowed defining limits of oscillation index in dump areas and in clean (control) native areas. The obtained results of the investigation indicate the soil depth, duration of experiment and type of area influence on soil invertase activity with the high statistical level of significance. Positive influence is revealed on invertase activity changing on dump areas, where an active excretorial and fossorial activity of phytophagous mammals was observed.
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SSR based characterization of peach (Prunus persica L.) and apricot (Prunus armeniaca L.) varieties cultivated in Hungary
17-24Views:263The SSR (Simple Sequence Repeat) markers allow the discrimination of the cultivars and determination its specific DNA fingerprints. The aim of this research was to evaluate fifteen apricot (Prunus armeniaca L.) and fifty-one peach (Prunus persica L.) genotypes cultivated in Hungary to obtain their DNA fingerprints in 6 SSR (Simple Sequence Repeats) loci by allele numbers and sizes.DNAs were extracted from leaves. PCR was carried out with CY-5 fluorescent labeled Prunus microsatellite markers and the products were separated on polyacrylamide gel with ALF (Automated Laser Flourometer)-Express II.According to our results, in the case of peach genotypes, all 6 SSRs were able to amplify alleles. UDP 96 005 was the most informative marker and UCDCH 17 was the least due to its monomorphic pattern. Regarding the apricot samples BPPCT 041 did not amplify any allele. In the case of P. armeniaca UDP 96 005 had the highest heterozygosity index as well and the highest number of alleles. The least informative marker was the UCDCH 17. Since the 6 SSR were not enough to discriminate the apricot and peach genotypes, it is suggested to use more SSR primers. -
Application of AFLP-Method in Plant Sample Identification
207-213Views:62One possible method for the determination of DNA-polymorphism is the PCR-based AFLP (Amplified Fragment Length Polymorphism). This method had been succesfully introduced to the Department of Botany at University of Debrecen in 2000-2001 with the examination of hay saffron (Crocus sativus L.) and its allies. Hay saffron is grown as a spice for some thousand years producing the most expensive spice in the world. This plant is sterile, triploid reproduces only vegetatively with no fertile seeds. However its origin is unknown it exists only in cultivation and it is a mutated variety of another species or an artificial or natural hybrid. Usual methods for the systematic examination are restricted hence it seemed to be reasonable to apply molecular biological methods in its case. Results of this work include the introduction and many fold application of the method beside ensuring the consequences of science literature with determining the C. cartwrightianus to give the most similar genetical pattern to C. sativus.
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Determination of the validation parameters of inductively coupled plasma mass spectrometer (iCP-mS): response curve linearity in the case of arsenic and selenium
67-71Views:131In the field of elemental analysis inductively coupled plasma mass spectrometers (ICP-MS) have the best sensitivity that means the lowest limit of detection, subsequently their applicability for the detection of essential and toxic elements in foods and foodstuffs is prominent. For the most elements could be measured the detection limit is between μg kg-1 (ppb) and ng kg-1 (ppt) e.g. for arsenic and selenium.
Considering an analytical task (sample type, analytes and their concentration, pretreatment procedure etc.) the applicability of an analytical method is determined by its performance characteristics. The purpose of validation is to ensure that the method would be used fulfills the requirements of the given task. In this article we describes one of the performance characteristics, the linearity, and the whole validation procedure aims measurement of arsenic and selenium in foodstuffs by inductively coupled plasma mass spectrometer (Thermo XSeries I.); but because of the limited number of pages the results are demonstrated only for arsenic.
The linearity of calibration was evaluated in three concentration ranges (0.1–1 μg l-1; 1–10 μg l-1; 10–50 μg l-1), with nine line-fit possibilities (without weighting, weighting with absolute or relative deviation; with or without forcing the curve through blank or origin) and different methods (graphical examination, correlation coefficient, analysis of variance).
The best method to ensure the linearity of correspondence between signal and concentration was the ANOVA test. In view of calibrations it was found that the range of 10–50 μg l-1 could be regarded as linear with four line-fit possibilities, and was non-linear between 0.1–1 μg l-1 and 1–10 μg l-1.
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Product orientated sewage sludge composting technologies in the practice of AKSD Ltd.
33-36Views:104In recent years the regulations of the EU unambiguously determine that the biodegradable wastes should be used for biogas production or composting. The direct use of sewage sludge in agriculture is limited by its hazardous microelement content, but it can be used effectively in composting. The limitation parameters can be extinguished with correct compost-mixture and technology variants. The exact determination of the technologies and mixtures result a stable, constant quality product. The compost, as a classified product, is friable structured. The end-product is a
homogeneous mould with good water capacity, does not contain any weed seeds and pathogenic organisms. The compost does not have a acidify effect on the soil. It is an effective fertilizer thanks to its macro and microelement content. -
Survival Analysis of Hungarian Large White, Duroc and Pietrain Sows
31-36Views:228The aim of the present study was to perform lifetime performance analysis in three pig breeds; Hungarian Large White (n=295), Duroc (n=76) and Pietrain (n=91) on a commercial farm using analysis of survival sows. We took into consideration the age of sows at the time of their inclusion into breeding, their age at the time of culling, time spent in production, number of mating and parities, parity percentage, intervals between litters, number and mean of piglets born alive and born dead, number of raised piglet litters, number and mean of 21 days old piglets, the weight and mean of raised litter and raise percentage.
We carried out the analysis by SPSS 22.0. Single factor analysis of variants, Kaplan-Meier analysis and Cox PH model were used. The determination of the significance of risk rates differences was done by Wald chi square test.
Our results showed that the average culling age were 1056 (±33.52) days for the Hungarian Large White, 735 (±73.56) days for Duroc and 818 (±71.98) days for the Pietrain.
The log rank test of the survival analysis indicated a significant difference between the three tested genotypes (χ2=16.981, P<0.001), which means that the survival percentage of the individual breeds varied significantly from one another. In comparison with the Hungarian Large White genotype the Duroc genotype has a 1.6 times higher (P<0.001) culling risk while that of the genotype Pietrain was 1.36 times higher (P<0.001).
Our results can be used to compare the breeds kept under the same conditions and to compare the life span of one genotype under different farming conditions. Factors that increase survival and improve the profitability of pig farming can be determined by this method.
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Evaluation of water balance in apple and pear trees
193-198Views:102A significant proportion of the aboveground green and dry weight of the plant constitutes the foliage. The canopy is an important factor
of plant growth. On one hand the canopy absorbs the solar energy, which is necessary for the photosynthesis, on the other hand accumulates
the absorbed nutrients by the roots, and the most of the water-loss happens through the foliages. The determination of the full canopy is not
an easy target. In our research we developed a measurement method to determine the leaf area. With the parameters of the examined tree
(leaf length and maximum width) and the data of ADC AM 100 leaf area scanner we determined the k-value, with which we can easily and
fast evaluate the leaf surface. Furthermore we defined from the water balance of compensation lysimeters the cumulative transpiration of
fruit trees and the efficiency of water use of trees. -
Water managed properties of apple and pear trees based on lysimeters
129-132Views:135A significant proportion of the aboveground green and dry weight of the plant is constituted by foliage. The canopy is an important factor of plant growth. On the one hand, the canopy absorbs solar energy, which is necessary for photosynthesis; on the other hand, it accumulates the nutrients absorbed by the roots, and most of the water-loss occurs through the foliage. The determination of the full canopy is not an easy target. In our research, we developed a measurement method to determine the leaf area. With the parameters of the examined tree (leaf length and maximum width) and the data of the ADC AM 100 leaf area scanner, we determined the k-value, with which we can easily and fast evaluate the leaf surface. Furthermore, we defined from the water balance of compensation lysimeters the cumulative transpiration of fruit trees and the efficiency of water use of trees. From the examined trees were made a 3D depiction, which show the shape, branching and the location of trees.