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Sequence stability at SSR, ISSR and mtDNA loci of common millet (Panicum miliaceum) from the middle ages
10-19Views:86Seed remains of medieval millet, recovered from a 15th century layer (King’s Palace, Budapest, Hungary), showed reddish yellow grain color after rehydrating on tissue culture medium that was close to grain color of modern cultivar Omszkoje. aDNA of medieval c. millet was extracted successfully, analyzed and compared to modern common millets by ISSR, SSR, CAPS and mtDNA. Analyses of fragments and sequences revealed
polymorphism at seven ISSR loci (22 alleles) and at the 5S-18S rDNA locus of mtDNA. CAPS analysis of the 5S-18S rDNA fragment revealed no SNPs in the restriction sites of six endonucleases TaqI, BsuRI, HinfI, MboI, AluI and RsaI. Sequence alignments of the restriction fragments RsaI also revealed
consensus sequence in the medieval sample compared to a modern variety. Morphological characterization of twenty common millet (Panicum miliaceum L., 2n=4×=36) cultivars and landraces revealed four distinct clusters which were apparently consistent with the grain colors of black, black and brown, red, yellow, and white. In the comparative AFLP, SSR and mtDNA analysis modern millet cv. ‘Topáz’ was used. AFLP analysis revealed that extensive DNA degradation had occurred in the 4th CENT. ancient millet resulting in only 2 (1.2%) AFLP fragments (98.8% degradation),
compared to the 15th CENT. medieval millet with 158 (40%) fragments (60% degradation) and modern millet cv. ‘Topáz’ with 264 fragments (100%). Eight AFLP fragments were sequenced after reamplification and cloning. Microsatellite (SSR) analysis at the nuclear gln4, sh1, rps28 and rps15 loci of the medieval DNA revealed one SNP (single nucleotide polymorphism) at the 29th position (A to G) of rps28 locus compared to modern millet.
Mitochondrial (mtDNA) fragment (MboI) amplified at the 5S-18S-rDNA locus in the medieval millet showed no molecular changes compared to modern millet. The results underline the significance of survived aDNA extraction and analysis of excavated seeds for comparative analysis and molecular reconstruction of ancient and extinct plant genotypes. An attempted phenotype reconstruction indicated that medieval common millet showed the closest morphological similarity to modern millet cultivar Omszkoje. -
Effect of floor type and bedding system on sows lifespan
155-160Views:105The paper studies two nucleus pig farms in the Great Plain region of Hungary which have same management, same feeding system but different breeding technology (Farm A had solid floor with straw and Farm B had slatted floor without straw). The genetics of sows were crossbred Dutch Large White and Dutch Landrace. The comparative examination was based on the causes of culling according to the different floor type. To the analysis of culling reasons was applied the methods of survival analysis.
In the analysis there were used the Kaplan-Meier method and the Cox Proportional Hazard model to the estimate the difference between the farms. The results of survival analysis showed significant difference (p<0.05) between the lifespan of sows that caused the difference between the survival probabilities of sows culled due to lameness. Furthermore there was showed that the sows kept on solid floor with straw have stayed in production longer than on slatted floor without straw. The hazard ratio was 1.434 that means that the culling due to lameness is 1.434 times higher by the slatted floor without straw facing by solid floor with straw.
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Comparative analysis of soil analysing datas on different sempling-plots
85-90Views:64Hibrid maize is cultivated on larger plots, therefore the sown areas of hibrid maize are heterogeneous from a pedology aspect. Heterogenity causes problems during tasseling, chemical plant protection and harvest. The heterogenity of sown areas can be compensated by fertilization which is based on soil analysis. We carried out research into change of the soil on four soil types from 1987 to 2005.
There were no significant changes in pH, hydroiodic acidity, CaCO3-content, humus-content on meadow chernozem soil. We detected equalization of salin content in the examined soil layers. There were no significant changes in the measured values on chernozem meadow soil and solonetz meadow soil in 2005. We discoverd equalization of saline content on chernozem meadow soil, but the changes were not as obvious as the changes on meadow chernozem soil. We found salinization in the 30-60 cm soil layer on type meadow soil that may be due to water movement. -
Analysis of changes in resources and outputs of companies and partnerships in Hungarian agriculture
65-68Views:83The aim of the study was to examine the achievement of Hungarian agricultural companies and partnerships, particularly agricultural co-operatives based on the aggregated database of National Tax and Customs Administration (NAV)1. From the methodological aspect, descriptive statistical methods and time series analysis were used. One of the most important conclusions is that the socio-economic weight of the agricultural co-operatives was strongly decreased in the period after the EU accession. The other important statement is that apart from the general examination of economic actors on aggregated level the comparative analysis of the single organizations forms would be needed. In the case of co-operatives, separated examination of the former type producer co-operatives and the so-called new type ones (e.g. marketing co-operatives etc.) would be necessary.
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Comparative analysis in residential property price level and price dynamics in urban and rural areas of Hajdú-Bihar County
85-90Views:113In modern market economies residential real estate prices, price shifts and their correlations with macroeconomic factors are surveyed quite frequently. However, in contrast with the wide scope of foreign examples, so far existing analyses in Hungary have ignored examining relation and extensity of macroeconomic indicators and failed to examine their effect on real estate pricing. The scope of this survey is to highlight these potential correlations and thus develop new aspects of analysis. Although the examination needs further extension both in time and space, the results of this survey may help to understand the importance of the responsible management of the most precious element of national wealth from the perspective of sustainable rural development.
Based on my preliminary results, there exists a strong correlation between the number of inhabitants of a settlement and the average real estate prices. Nevertheless, the correlation seems to be significant only for cities. In the case of smaller settlements the correlation still exists but at a lower level. As opposed to the results of former publications and my own expectations, no direct link could be tackled between the amount of income tax paid by private individuals and real estate prices either in the cities or in the villages within the territory and time span examined in my analysis. Although this correlation is measurable on a macro-economic level, my micro-regional analyses revealed the complexity of asset pricing and price volatility. Continuing this survey, my goal is to identify the hidden factors influencing real estate prices, whose thorough mapping may promote conscious rural development.
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Comparative analysis of maize weed control system and the competitive effect of sorghum
97-104Views:82In our investigation we used different weed control technologies in the different phenology states of the maize. The experiment have been
carried out in Hódmezővásárhely, in the Experiment garden of the Pilot farm of University of Szeged Faculty of Agriculture, on meadow
chernozem soil, on 24 m2 plots, in 3 replications, randomized blotch design. The experiment can be regarded as 15 weed-control strategies
where, in addition to the untreated control, six chemicals or chemical-combinations are applied (Spectrum 720 EC, Motivell Turbo D,
Stellar + Dash HC, Clio + Akris SE + Dash HC, Clio + Dash HC) in five different times (pre, early post, post and two late post) and eight
mechanical weed-control technologies were used. Hoeing took place connected to the herbicide treatments in different times: until 2-3-leave
age weedless, in 3-4-leave age hoed once, from 3-4-leave age weedless, in 6-7-leave age hoed once, from 6-7-leave age weedless, in 8-leave
age hoed once, from 8-leave age weedless.
Our results were assessed by chemical efficiency examination, maize length measurement, corncob-length and fertility examination,
Sorghum plant-number determination and yield weighing carried out in four periods. The data were evaluated by a one-factor analysis of
variance and a two-factor linear regression analysis. -
Comparative analysis of Carpathian Braunvieh’s morphological traits
15-19Views:176The Carpathian Braunvieh cattle established by the cross-breeding of the Schweizer Braunvieh and the local breeds of the Carpathian basin approximately 150 years ago. The evolved three usage breed was durable and resistant, however in comparison with the high-productivity breeds was less competitive. The dramatic lay-off, and the endangered status of the breed requires a conservation programme. University of Debrecen took the investigation of the Hungarian population and it’s detailed genetic studies. Within this research project among others we carry out body measurements and rates of the body traits. Depending on these results, we may decide on the subsequent gene-reserving objects. Based on body measurements and live weight we established that the breed at Mikóháza favours to the one lived in Hungary in the 1960’s, and to another, named Ukrainian Carpathian Brown. Compared to the earlier data (Horváth, 1966) smaller body traits can be seen. Our livestock is proportionately smaller than the other breeds in height at withers, as well as in live weight. Based on the comparative analysis, we established, that – thanks to the aware sorting – the imported individuals represent the ancient, primitive Carpathian variant.
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Comparative analysis on the fertiliser responses of Martonvásár maize hybrids in long-term experiments
111-117Views:68The results of experiments carried out in the Agricultural Research Institute of the Hungarian Academy of Sciences clearly show that in the case of hybrids grown in a monoculture greater fertiliser responses can be achieved with increasing rates of N fertiliser than in crop rotations. In the monoculture experiment the parameters investigated reached their maximum values at a rate of 240 kg/ha N fertiliser, with the exception of 1000-kernel mass and starch content. In both cases the starch content was highest in the untreated control, gradually declining as the N rates increased. Among the parameters recorded in the crop rotation, the values of the dry grain yield, the 1000-kernel mass, the protein yield and the starch yield were greatest at the 160 kg/ha N fertiliser rate, exhibiting a decrease at 240 kg/ha. Maximum values for the protein content and SPAD index were recorded at the highest N rate. It is important to note, however, that although the N treatments caused significant differences compared to the untreated control, the differences between the N treatments were not significant.
In the given experimental year the values achieved for the untreated control in the crop rotation could only be achieved in the monoculture experiment at a fertiliser rate of 160 kg/ha N, indicating that N fertiliser rates could be reduced using a satisfactory crop sequence, which could be beneficial from the point of view of environmental pollution, crop protection and cost reduction.
The weather in 2006 was favourable for maize production, allowing comparative analysis to be made of the genetically determined traits of the hybrids. Among the three hybrids grown in the monoculture experiment, Maraton produced the best yield, giving maximum values of the parameters tested at a fertiliser rate of 240 kg/ha N. The poorest results were recorded for Mv 277, which could be attributed to the fact that the hybrid belongs to the FAO 200 maturity group, while the other hybrids had higher FAO numbers. Maraton also gave the highest yields in the crop rotation experiment at the 160 kg/ha N level. All three hybrids were found to make excellent use of the natural nutrient content of the soil.
It was proved that the protein content of maize hybrids can only be slightly improved by N fertilisation, as this trait is genetically coded, while the starch content depends to the greatest extent on the ecological factors experienced during the growing season. -
Sensitivity of methods for estimating reference evapotranspiration
51-56Views:129The knowledge of the evapotranspiration of natural ecosystems and plant populations is of fundamental importance in several branches of science, research topics and practical uses. Nevertheless, the harmonization of the large number of methods and the changing user needs often causes problems. Sensitivity analysis of 10 ET0 estimation models and model variants was performed. Magnitude of the obtained outputs and the changes triggered by each atmospheric parameter were evaluated. The objective of the analyses was to get to know the sensitivity of the different models and to select the most accurate and the most suitable ones for adaptation to local circumstances. Therefore, it becomes possible to achieve as high accuracy as possible in applications which need ET0 estimation.
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Examining the compensation for the 2010 flood in the Sajó valley using real estate examples
5-14Views:157The intention of this article is to investigate the central damage mitigation of the 2010 flood in point of properties. During the investigationI performed the processing of the revealed data on a country level perspicuity, and in details on the level of sample area. In my study I worked with the available damage summations, the number of the damaged properties and the sources granted by central budget to extenuate those,and the husbandry database comparative analysis of local government of the sample strata. It came clear by the analysis of the results, which regions require more acquisition in the further process of the project.
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Comparative analysis of Staphylococcus aureus strains by molecular microbiology methods
34-39Views:108Staphylococcus aureus is a very important pathogen for dairy farms and milk processing plants. Subclinical mastitis is often caused by this species, and it can contaminate bulk tank milk when milking cows are suffering from mastitis. Additionally, thermostable enterotoxins (SE) produced by some types of this bacterium can cause food poisoning.
The aim of our research was to examine the number of S. aureus in bulk tank milk in two dairy farms and the enterotoxin-producing ability, genetic relation (pulsotype) and antibiotic resistance of S. aureus strains from different sources (bulk tank milk, udder quarter milk and environment).
The results show that the mean number of S. aureus of bulk tank milk of two farms significantly differed (P<0.05). Fourteen isolates were selected for further molecular genetic studies (five isolates were from bulk tank milk and nine isolates were from udder quarter milk). S. aureus was not recovered from the environmental samples. Three of the fourteen isolates (21.4%) tested by multiplex PCR were positive for SE genes. Two isolates carried one gene (seb) and one isolate carried two genes (seg and sei). The fourteen strains were classified into three pulsotypes and two subtypes at 86% similarity level. Isolates from bulk tank milk (n=5), were divided into 2 pulsotypes (A, C) and one subtype (C1). The isolates from udder quarter milk (n=9) belonged to three different pulsotypes (A, B, C) and two subtypes (A1, C1). The distribution of pulsotypes in the present study revealed genetic relationship between S. aureus isolated from udder quarter milk and bulk tank milk. This could be explained by the fact that in farms with a high number of infected cows, these cows could represent the main source of contamination. The results of the antibiotic resistance investigations show, that all strains were susceptible to methicillin, cefoxitin, lincomycin, tetracycline, erythromycin and sulfamethoxazole/trimethoprim. Thirteen out of fourteen strains were resistant to penicillin (A and C pulsotypes, A1 and C1 subtypes) and just one isolate was susceptible (B pulsotype) to all antibiotics tested. -
Comparative analysis of maize hybrides grown for bioethanol production purposes
11-14Views:95The objective of this study was to evaluate the effects of crop year on the main components of maize grown for bio-ethanol production yield, starch content and starch yield per hectare of maize hybrids were investigated in a droughty (2007) and in a favourable years with optimal weather conditions (2008, 2009). We measured very low starch yield (1.5 t ha-1) in the year with unfavourable precipitation supply (2007) together with higher starch content (73%). In the case of good precipitation supply we measured a much higher starch yield per hectare (7.7 t ha-1) with lower content (72.4%). In 2009 the starch content (74.5%) exceed the results of the two previous years, but on the other hand the average of starch yield was (4.9 t ha-1) which falls between the other two years values.
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Comparative analysis of sample preparation methods to determine the concentration of arsenic in soil- and plant-samples
167-170Views:130Arsenic contamination of the fields and groundwater is a global problem. Alföld is the most affected area in Hungary. Irrigation witharsenic contaminated water, and crop production on the contaminated soil can cause a food safety problem, because arsenic is easy taken up by the cell of the plant roots. To prevent this, very important to monitoring the arsenic content of soils and plants. Inductively coupled plasma mass spectrometry (ICP-MS) is a fast, easy method to determined the concentration of minerals in the case of plant and soil samples The analytical methods can give reliable, results if the analytical process, including the sample preparation method, is the best. The objective of this study was to compare 3 type of sample preparation method which was dry ashing, wet digestion in open system, and microwave digestion. As a result of our experiement shows the microwave digestion is the appropriate method to determined the arsenic content of soil samples. In the case of plant samples we can use wet digestion in open system or microwave digestion as a samle preparation method.
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The production and financial achievement of the agricultural co-operatives in 2002-2007
21-28Views:101The aim of the study was the examination of the achievement of the Hungarian agricultural enterprises – special regard to the agricultural co-operatives – based on the aggregated database of Hungarian Tax and Financial Control Administration (APEH), from the database of the Research Institute of Agricultural Economics. From the methodological aspect plain statistical devices were used, indicators for corporate analyses were
calculated and analyzed for the time series. One important conclusion is that the socio-economic weight of the agricultural co-operatives was strongly decreased in the period after the EU accession. The other important statement is that over the general examination of economic actors on an aggregated level the comparative analysis of the single enterprises forms would be needed. In the case of the co-operatives the separated examination of the former type producer co-operatives and the new type co-operatives would be necessary -
Comparative analysis of certain soil microbiological characteristics of the carbon cycle
137-141Views:128In our researches, we examine the soil microbial parameters related to the carbon cycle. In this study, we compare the changes of microbial biomass carbon (MBC) and the soil CO2 production in soil samples which were taken in spring and autumn. The 30 years old long-term experiment of Debrecen-Látókép is continued in our experiments. The long-term fertilization experiment was set in 1983, and our sample was taken in spring 2014. The examinations of soil respiration processes and factors that influence soil respiration are required in optimal management. In our study, we interested to know how the growing levels of fertilization influence the soil respiration and microbial biomass carbon under non-irrigated and irrigated conditions in maize mono, bi, and triculture.
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Comparative analysis of physiologically important fatty acids
71-76Views:101Fatty acid composition of some vegetable oils, like wheat germ, walnut, peanut, hempseed, linseed, sunflower-seed, olive, rapeseed, grape seed or pumpkin seed, analysed at Food Science Institute of Debrecen University, are summarised here. The effect of heat treatments usually used in Hungarian cuisine was examined in this paper.The influence of different fatty acids on human health is also reviewed.
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Comparative study of a winter wheat variety and hybrid sown after different pre-crops on chernozem soil
63-69Views:205Wheat production is a determining branch within Hungarian crop production (produced on nearly one million hectares). Weather anomalies caused by climatic change confirmed the importance of the biological background (variety, hybrid) in wheat production. The adapting ability and reaction of different wheat genotypes towards nutrient supply were studied in a long-term field experiment on chernozem soil type in the case of different pre-crops (sunflower and maize). According to the experimental results of the vegetation of 2017/2018, the yield of the variety Ingenio sown after the sunflower as previous crop ranged between 4168 and 8734 kg ha-1, while in the case of maize as previous crop, this value ranged between 2084 and 7782kg ha-1, depending on the applied nutrient supply level. The studied genotypes produced rather significant yield surplus as a response to the application of mineral fertilization (4.6–5.1 t ha-1 after sunflower and 5.7–6.3 t ha-1 after maize). Optimal mineral fertilizer dosage was determined by both the genotype and the pre-crop. N-optimum values of wheat genotypes was determined using regression analysis. In the case of the variety Ingenio sown after sunflower, the optimum range was N144-150+PK, while after maize, it was
N123-150+PK, respectively. For the hybrid Hyland, these optimum ranges were N114-120+PK, just as N150-153+PK, resp. The application of optimal mineral fertilizer dosages improved water utilization of the studied wheat genotypes to a significant extent. WUE values of the control, unfertilized treatments ranged between 4.1–8.3 kg mm-1, while in optimal fertilizer treatment, it ranged between 15.5 and 17.4 kg mm-1. -
Comparative study of newly-bred black locust clones with regard to photosynthetic rate and water use efficiency: early evaluation
5-10Views:199Black locust (Robinia pseudoacacia L.) is one of the most important tree species in Hungary, due to its positive economic impacts. Research to increase its yield, improve its stem quality and enhance its drought tolerance has been ongoing since the 1960s. Of the current research works in this field, the clone trial of the Forest Research Institute, University of Sopron, established in 2020 in the Nyírség region, is worth highlighting. In this experiment 4 newly-bred clones and a state-approved black locust cultivar ('Üllői') are being tested. In the summer of 2022, ‘on site’ measurements of assimilation parameters – net assimilation (An), transpiration (Tr) – were carried out using the LI-6800 portable photosynthesis system. From the data obtained, the water use efficiency (WUE) was calculated. The results of the statistical analysis (Kruskal-Wallis H test) have shown significant differences (p < 0.05) between the clones for all three parameters (An, Tr, WUE) tested. The NK2 clone has performed the highest value for all the parameters studied. However, no significant differences were found between clones NK2 and PL040 for Tr or between NK2 and control ('Üllői') for WUE. Studies of this kind contribute to the improvement of black locust growing through the production and selection of cultivars, which are relatively resistant to the negative effects (drought) of climate change.
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The significance of biological bases in maize production
61-65Views:159The comparative trial has been set up in the Demonstration Garden of the Institute of Crop Sciences of the University of Debrecen, Centre for Agricultural and Applied Economic Studies, Faculty of Agricultural and Food Sciences and Environmental Management in 2012, with 24 hybrids with different genetic characteristics and growing periods. The soil of the trial is lime-coated chernozem, with a humus layer of 50–70 cm.
The weather of the trial year was quite droughty; the monthly average temperature was 3–4 oC higher than the average of 30 years. High temperature, together with lack of precipitation occurred during the most sensitive phenophases of maize (flowering; fecundation, grain saturation).
The following characteristics have been observed: starting vigour, date of male and female flowering, plant and cob height, dry-down dynamics during maturation and the change of yield composing elements has also been quantified. The yield was recalculated to 14% moisture content grain yield after harvesting.
The beginning of the growing period was advantageous, therefore the analysed hybrids could grow a high (above 300 cm) and strong stem. The yield of the hybrids changed between 10.33 and 11.87 t ha-1, but as a result of the unfavourable climatic extremes, their genetic yield potential prevailed only at a rate of 30–40%. However, moisture content by the time of harvesting was good despite its early date (12th September); it remained under below 14% in most cases. Dry-down was measured on a weekly basis between 14th August and 5th September.
The analysis of the qualitative parameters of the maize hybrids (protein %, oil % and starch %) resulted in significant differences. The most significant difference has been observed in the case of protein content (LSD5%=2.01). Oil content was the most advantageous in the case of hybrids belonging to the mid-late growing group (FAO 400). The X9N655 and 36V74 hybrids had the highest oil content (around 4%), while hybrids P9915 and 37F73 had significantly lower oil content. Starch content was above 70% in the case of every hybrid.
Hybrid selection is highly important in terms of yield and yield security of maize, as well as the application of modern biological fundamentals and hybrid specific technology for the improvement of the level of cultivation technology.
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Comparative analysis of the transformation of Hungarian and East German agriculture
164-170Views:76Agriculture, and within it, the development and later transformation of the cooperative system shows many similarities between Hungary and the Eastern German provinces. A few examples can be mentioned, such as the mistrust against the notion of cooperation, the forced development of the cooperatives and, as an occupied territory, the influence of the Soviet Union. Similar issues emerged in both countries’ agriculture and the measures taken were also alike. Similar social, economic processes and changes were started at the end of the 1980s (1989/90) and these had a significant effect on the areas that serve as a basis for this study.
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Comparative analysis of inoculated soybean in extinguishing soil
113-116Views:195In order to achieve higher yields, better technological methods offered in the current market, which aims to help the producers in the realization. To guarantee good yield because more and more people try with a variety of products, but you have to make the expected impact falls short.In this experiment, the following results were obtained: the soil suspension closely related to the inoculum of seed or seed treatment,which affects the soybean nodule formation, core saturation, and yield and protein-oil indicators. The soil suspension composition may exert positive and negative effects, which depend on how bacterial strains inoculum combined on the soybean seed surface. It is shown in our experiment very well, that between inoculum and inoculum as well as inoculum and seed treatment materials may be antagonism which setback to the soybean nodules formation and the yield, but not worsen the protein-oil indicators.