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Studies of the effects of N fertilizers and Microbion UNC biofertilizer on microelement content of horseradish (Armoracia macrocarpa)
41-45Views:115A field experiment on calcareous chernozem soil was performed to study the effects of different N and bacterial fertilizers on the nutrient content of horseradish (Armoracia macrocarpa). In the experiment the trials were arranged in a randomized block design with three replications, applying three levels of NH4NO3 and different N fertilizers, namely ammonium-nitrate, urea and calcium-nitrate, with or without application of Microbion UNC biofertilizer.
In the present paper the changes and distribution of manganese, zinc and copper contents of the horseradish plant are summarized by the
effect of different treatments.
The Mn content of leaves were higher in all cases than those of roots, but Zn mainly accumulated in the roots. The distribution of copper within the horseradish plant was more equalized than that of Zn and Mn. Different N fertilizers and increasing doses of ammonium-nitrate had effects mainly on the microelement contents of leaves. The highest Mn contents of plant were measured in treatments of Ca(NO3)2 and Ca(NO3)2+Microbion. The lowest ammonium nitrate dose (N1) decreased the Mn content of leaves compared to control, but further doses
(N2, N3) did not alter these values any longer. Microbion UNC biofertilizer did not have any effect on the Mn content of roots, but we measured higher Mn in leaves in some combined treatments. Ca(NO3)2 increased the zinc content in leaves and roots in a noticable manner. With the increasing of NH4NO3 doses, the Zn content of leaves and roots augmented significantly. Neither N fertilizers (or the increasing doses of NH4NO3) nor the biofertilizer application influenced the Cu content of horseradish plant.
N fertilizers had higher effects on the microelement content of horseradish, the biofertilizer’s effect was smaller and was not the same in every treatment. -
The effect of the queen's age on the Varroa mite (Varroa destructor) burden of honey bee (Apis mellifera L.) colonies
83-87Views:275An apiary trial was conducted in 2016 August to October in Szabolcs-Szatmár-Bereg County, Nyírmada to evaluate the influence of queen’s age on the Varroa destructor-burden in the treatment colonies. Sixty colonies of bees belonging to the subspecies Apis mellifera carnica pannonica in Hunor loading hives (with 10 frames in the brood chamber/deep super) were used. The colonies were treated with amitraz and the organophosphate pesticide coumaphos active ingredients. The amitraz treatment includes 6 weeks. The coumaphos treatment with Destructor 3.2% can be used for both diagnosis and treatment of Varroasis. For diagnosis, one treatment is sufficient. For control, two treatments at an interval of seven days are required. The colonies were grouped by the age of the queen: 20 colonies with one-year-old, 20 colonies with two-year-old and 20 colonies with three-year-old queen. The mite mortality of different groups was compared. The number of fallen mites was counted at the white bottom boards. The examination of spring growth of honey bee colonies has become necessary due to the judgement of efficiency of closing treatment. The data was recorded seven times between 16th March 2017 and 19th May 2017.
Data on fallen mites were subjected to one-way analysis of variance (ANOVA) and Post-Hoc Tukey-test. Statistical analysis was performed using the software of IBM SPSS (version 21.). During the first two weeks after treatments, the number of fallen mites was significantly higher in the older queen’s colonies (Year 2014). The total mite mortality after amitraz treatment in the younger queen’s colonies was lower (P<0.05) compared to the three-year-old queen’s colonies. According to Takács and Oláh (2016) although the mitemortality tendency, after the coumaphos (closing) treatment in colonies which have Year 2014 queen showed the highest rate, considering the mite-burden the colonies belongs to the average infected category. The colonial maintenance ability of three-year-old queen cannot be judged based on the influencing effect on the mite-burden. The importance of the replacement of the queen was judged by the combined effect of several factors.
During the spring-growth study (16th March–19th May) was experienced in the three-year-old queen’s colonies the number of brood frames significantly lower compared to the one- and two-year-old queen’s colonies. In the study of 17th April and 19th May each of the three queen-year-groups were varied. Therefore in the beekeeping season at different times were determined the colonial maintenance ability of queens by more factors: efficiency of closing treatment in early spring, the spring-growth of bee colonies, the time of population shift (in current study, this time was identical in each queen-year), honey production (from black locust).
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The efficacy of combining paraffin oil with conventional fungicide treatments against grape powdery mildew in Eger
173-180Views:111We aimed to test the combination of paraffin oil (PFO) with regular fungicide treatment to assess its efficacy against grape powdery mildew (GPM) in a small spraying experiment on two Vitis vinifera L. cultivars (Chardonnay and Kékfrankos) with different susceptibility to Erysiphe necator. The visual symptoms of GPM on leaves and clusters were examined at three phenological states. The harvest yield was characterized by two methods, data were analyzed with one-way ANOVA and Tukey post-hoc test. Regular fungicide treatment (CT) and its combinations with PFO showed better results in both varieties to repress GPM in 2015 relative to sole PFO treatments. Mean values of combined treatments were often lower than CT but did not differ significantly from each other. The same was observed in 2016, despite the higher pressure of GPM, and missed the third survey. No significant differences were detected between treatments in yield. In contrast, the mean cluster weight of CT and combined treatments resulted in (insignificantly) higher values in each variety and year. In summary, the sole PFO showed some disease control capability as reported earlier, but this effect was greatly affected by the given vintage. Combining PFO with CT resulted in increased protection against GPM relative to the solely applied fungicides. However, this effect was not significant in all cases. It also depended on the vintage and cultivar characteristics. The beneficial impact of paraffin oil as an additive to CT may be due to the induction of plant stress responses and/or its ability to support the adherence and absorption of the combined agents.
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Comparative examination of a mineral fertiliser and a bacterial fertilizer on humic sandy soil
111-116Views:79In our pot experiment, the impact of a bacterial fertilizer, Bactofil® A10 and a mineral fertilizer Ca(NO3)2 applied in different rates was studied on some soil chemical and microbiological characteristics of a humic sandy soil (Pallag). Perennial rye-grass (Lolium perenne L.) was used as a test-plant. Samples were collected four and eight weeks after sowing in each year. The experiment was set up in 2007-2009 in the greenhouse of
the UD CASE Department of Agrochemistry and Soil Science. The available (AL-extractable) nutrient contents of soil, among the microbial parameters the total number of bacteria, the number of microscopic fungi, cellulose-decomposing and nitrifying bacteria, the sacharase and urease enzyme activity, as well as the soil respiration rate were measured.
Statistical analyses were made by means of the measurements deviation, LSD values at the P=0.05 level and correlation coefficients were calculated. Results of our experiment were summarised as follows:
− The readily available nutrient content of humic sandy soil increased as affected by the treatments, in case of the available (AL-extractable) phosphorus and potassium content the higher value was measured in high-dosage artificial fertilizer treatment.
− The treatments had also positive effect on several soil microbial parameters studied. The higher-dosage mineral fertilizer treatments had a beneficial effect on the total number of bacteria, cellulose-decomposing and nitrifying bacteria. No significant differences were obtained between the effect of treatment in case of the total-number of bacteria, the number of microscopic fungi and nitrifying bacteria.
− On the sacharase enzyme activity the artificial fertiliser treatments proved to be unambiguously stimulating, the urease activity significantly increased on the effect of the lower-dosage Ca(NO3)2 artificial fertilizer treatment.
− The soil respiration increased in all treatments in related to the amounts applied, significantly increased in the highest rate of Ca(NO3)2 fertilizer addition.
− Some medium and tight positive correlations were observed between the soil chemical and microbiological parameters studied in case of both nutrient sources.
Summarizing our results, it was established that the organic and all the mineral fertilizer treatments had beneficial effects on the major soil characteristics from the aspect of nutrient supply. In majority of the examined soil parameters (AL-extractable phosphorus- and potassium, total number of bacteria, number of cellulose-decomposing and nitrifying bacteria, activity of sacharase enzyme) the high rate of Ca(NO3)2 mineral fertilizer treatment proved to be more stimulating, but at the same time the high rate bacterium fertilizer resulted in significant increases in
the nitrate-N content, the AL-potassium content of soil, the total number of bacteria, the number of cellulose-decomposing and nitrifying bacteria and the urease enyme activity.
Our examinations showed that the mineral fertilizer treatments proved to be more stimulating on most of the soil parameters studied but according to our results, it was established that Bactofil is efficiently applicable in the maintenance of soil fertility and the combined application of
mineral fertilizer and bacterium fertilizer may be a favourable opportunity – also in aspect of the environmental protection – in maintaining soil fertility. -
Effects of combined nutrient supply treatments on some physiological parameters of autumn wheat
241-251Views:137The Fleischmann Rudolf Research Institute in Kompolt is not only famous for plant breeding but the institute also surveys the effects of different nutrient supply methods since 1918. In 2017, we joined this research supported by EFOP 3.6.1 project. Our aim was to investigate photochemical processes – which is one of the most determinant in case of yield – of crops by in vivo field measurements. We measured the chlorophyll content of leaves using Minolta SPAD 502. We used miniPAM fluorometer to determine actual photochemical efficiency and non-photochemical quenching of PSII during natural light conditions and also to evaluate the pigment (chlorophylls and carotenoids) and water content of leaves we applied field spectrophotometer (ASD FieldSpecPro 3). We utilized these methods by various treatments (1. treatment with soil bacteria + head and base fertilizer; 2. treated by only head fertilizer; 3. treated by only base-fertilizer) in field experiment of autumn wheat (4.1–2.43–1.19 ha) in June, 2017. The difference between treatments was clearly detectable. In the case of the first treatment, physiological processes were more intense and the ripening occurred earlier. The obtained yield was the highest in the case of the area treated by soil bacterial. Based on the results, the first treatment can be recommended in practice.
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Comparative analysis of inoculated soybean in extinguishing soil
113-116Views:195In order to achieve higher yields, better technological methods offered in the current market, which aims to help the producers in the realization. To guarantee good yield because more and more people try with a variety of products, but you have to make the expected impact falls short.In this experiment, the following results were obtained: the soil suspension closely related to the inoculum of seed or seed treatment,which affects the soybean nodule formation, core saturation, and yield and protein-oil indicators. The soil suspension composition may exert positive and negative effects, which depend on how bacterial strains inoculum combined on the soybean seed surface. It is shown in our experiment very well, that between inoculum and inoculum as well as inoculum and seed treatment materials may be antagonism which setback to the soybean nodules formation and the yield, but not worsen the protein-oil indicators.
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Experiments on the Nutrient Removal and Retention of an Integrated Pond System
18-23Views:183A combined intensive-extensive fishpond system developed for the purification and re-use of intensive fishpond effluent water was studied during a three-year experimental period. The investigated pond system consists of five small-size intensive culture ponds of 1 ha total water surface area with 1.5 m water depth and a 20 ha extensive culture pond with 1.0 m average water depth. The water was recirculated between the intensive and extensive ponds with around 60 days retention time in the extensive treatment pond.
Carbon, nitrogen and phosphorus budget and water purifying capacity were described and evaluated by means of regular measurements of nutrient concentrations in the water and sediment. During the three-year test period, 81.5% of organic carbon, 54.7% of nitrogen and 72.2% of phosphorus were retained by the system as a percentage of the total input of each nutrient. A significant amount of the total nitrogen input was removed by the harvested fish, which was much higher than in traditional fishponds or intensive fish culture systems. The efficiency of nutrient removal is clearly indicated by the 27.3% nitrogen assimilation.
Only a small percentage of the total nutrient input was discharged into the environment during fish harvest, which was 9.0% for organic carbon, 13.2% for nitrogen and 12.1% for phosphorus. The combination of intensive and extensive fishponds with water recirculation resulted in significant reduction of nutrient discharge into the surrounding aquatic environment, primarily due to the high nutrient processing and retention capacity of the extensive fishpond ecosystem. -
Studies of the influences of different N fertilizers and Microbion UNC bacterial fertilizer on the nutrient content of soil
134-140Views:59A field experiment was conducted to examine the effects of different nitrogen fertilizers in combination with bacterial fertilizer on
nutrient uptake of horseradish and plant available nutrients of the soil. Three different N fertilizers, ammonium-nitrate, urea and calciumnitrate
(116 kg ha-1 N) in combination with Microbion UNC bacterial fertilizer (2 kg ha-1) were applied as treatments in a randomized
complete block design in three replications. In this paper we presented the results of soil measurements. The soil of the experimental area
was chernozem with medium sufficiency level of N and P and poor level of K.
Our main results:
The amount of 0.01M CaCl2 soluble inorganic nitrogen fractions, NO3
--N and NH4
+-N and also the quantity of soluble organic-N were
almost the same in the soil. N fertilizers significantly increased all the soluble N fractions. The amount of NO3
--N increased to the greatest
extent and the increase of organic N was the slightest. We measured the largest CaCl2 soluble NO3
- -N and total-N contents in the plots
treated with ammonium-nitrate, the largest NH4
+-N in the plots treated with calcium-nitrate and the largest organic-N fraction in plots
treated with urea.
Bacterial inoculation also increased both soluble inorganic nitrogen forms and also total-N content of soil compared to the control. In
the case of combined (artificial and bacterial fertilizer) treatments we measured lower NO3
--N, organic-N and total-N compared to the
values of plots having only nitrogen fertilizer treatments. On the contrary in the plots with combined treatments the CaCl2 soluble NH4
+-N
content of soil in more cases were higher than that of values with artificial fertilizer treatment.
As a function of calcium-nitrate application increased AL-P2O5 and AL-K2O values were measured compared to control. Microbion
UNC supplement of calcium nitrate yielded also increase in AL-P2O5 and AL-K2O values, till then supplement of ammonium-nitrate fertilizer
yielded a decrease in these values compared to the control.
All nitrogen fertilizers resulted in a significant decrease in AL-Mg content of soil compared to the control. Nevertheless bacterial
fertilizer increased AL-Mg values in any cases. -
Measuring of nitrogen leaching using ceramic suction cups at different locations
10-17Views:71Ceramic suction cups were used for the measurement of N-concentration in soil solutions under different soil and climate conditions in both field experiments of Rostock University and Agricultural University of Debrecen (Hungary). Depending on the soil utilisation the change in the N concentration of the soil solution can be proved on both sites.
The experimental field of Rostock University can be characterised by its high groundwater table. The nitrogen concentration of soil solutions in the different soil layers were determined by the trend downward of water. In the dactylis (Dactilis glomerata) experiment, the quadruple treatments involved the following: with and without N-fertiliser, with and without harvesting, respectively. In the lower soil layers, the least rising N concentrations were established in case of the treatment without N-fertiliser combined with harvesting. The nitrogen leaching calculated from the infiltrated water quantity and the nitrate N concentration increased in the following order: without N-fertiliser, with harvesting < without N-fertiliser, without harvesting < with N-fertiliser, with harvesting << with N-fertiliser, without harvesting.
The field experiment site of Debrecen can be characterised by a low groundwater table. The effect of N-fertilisation on the nitrate-N concentration of soil solution in the soil layers can be stated unanimously. Permanent nitrate-N leaching cannot be established due to the water upward movement under semiarid climate conditions. Intermittently transfer of nitrate-N between the soil layers is probable in cases of remarkable precipitation. -
Preliminary results of the combined production of duckweed Spirodela polyrhiza and common carp (Cyprinus carpio) in an aquaponic system
83-89Views:65As a result of population growth, increasin amount of food is needed, and agriculture is under an increasing burden to meet these needs. Traditional agriculture is often attacked due to its environmental impact. We must find alternative, environmentally friendly ways to produce more food. Aquaponics is a closed system in which we can produce both fish and plants at the same time. Duckweed species are small, aquatic, floating plants belonging to angiosperms. It can potentially be an alternative protein source, due to its high protein content, good amino acid supply, and rapid growth. Under suitable conditions, it doubles its weight in 2–4 days, and can reach a yield of 30 t ha-1 year-1in dry matter. It forms a carpet on top of the water and can be found in slow-moving or still waters. Since they are resistant to a wide range of nutrient concentrations, they are also suitable for cleaning wastewater (such as eutrophicated lakes, sewage reservoirs, liquid manure storage). Fish feed is the primary nutrient source for aquaponic systems, which usually contains fishmeal. If duckweed can be used as an alternative for fishmeal in the feed, it could improve the sustainability of the aquaponic and aquaculture systems. In this study, the aim was to develop an optimal harvesting protocol for duckweed Spirodela polyrhiza under aquaponic conditions. In a four weeks experiments, four harvesting protocols were set up, a control where only biomass measurements were made, a 25% group where 25% of the biomass at the time of measurement was harvested, and a 50% and a 75% group where at the time of measurement 50% and 75% of the biomass was harvested. Three replicates were used per treatment. We weighed the biomass every week and removed the amount corresponding to the group. Based on the preliminary results, it can be said that more biomass was obtained in the groups with the 25% harvesting protocol and the control group.
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Examination of Possibilities for Increasing Sugar Content
202-206Views:33The result of sugar beet production is highly effected by the quality and the yield. Those agricultural techniques are very important which can be applied at low cost to improve these parameters. Such an opportunity is to increase sugar yield by foliar fertilization with nutrients and by treatment with bioactive fungicides.
In my small block trial I examined the effect of the magnesium and strobilurin active ingredients in different combinations and at different application times on the quality and the yield of sugar beet.
I found that there was a signifficant increase in sugar content on that blocks which were treated with a bioactive ingredient combined with magnesium in the middle of July and on those which were sprayed with bioactive ingredient in August.