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Evaluation of blue and red corn landraces
239-243Views:73Corn breeding can be successful only on a broad base of genetic material. The expansion of breeding aims includes the research of germplasm materials. In our experiments, we examined 11 blue, purple and red exotic corn varieties on two locations in Hungary. We conducted a complex study to obtain more information. We studied several morphological and phenological features and determined the most important qualitative parameters (protein, fat, ash). We applied the Tassel Area Index (TAI) under Hungarian conditions and compared it with the pollen producing ability of different genotypes. Results show that there are great opportunities in exotic corn varieties. Their quality exceeds that of the yellow ones in many cases. There are significant differences in yield and nutritional parameters. The favorable nutritional composition is not coupled with great productivity. Based on their flowering time and their agronomical features, they can be cultivated under Hungarian conditions as well. They match the new breeding aims, so they can be used as functional food or energy plants. With their high pollen producing ability and TAI, they can be utilized as markers in future pollen research.
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Investigation of Potato (Solanum tuberosum L.) Salt Tolerance and Callus Induction in vitro
51-55Views:90Potato production plays an important role in Hungary and the other countries of Europe. Consumption of potato products has increased to a large extent during the past several years. We can satisfy market demands with high quality and virus-free varieties.
Results of potato production depend on tolerance/resistance to abiotic stresses. In many cases, increased concentration of NaCl causes yield loss. Selection of salt tolerant varieties proved to be a difficult problem. Nowadays, the salt tolerance of potato varieties can be determined by cell/tissue/ protoplast techniques. Somaclonal variation provides a great potential for selection of lines resistant to salt stress. In vitro shoots and callus, derived plantlets selected for salt tolerance/resistance provide material for micropropagation.
In vitro shoot development of potato (Solanum tuberosum L. cv. Kuroda) was investigated under salt stress (40 mM, 80 mM, 120 mM NaCl) conditions. Shoot heights of plantlets cultured under salt conditions were lower than the control through the investigation. However, the shoot development of plantlets originated from in vitro meristems was almost at the same level as the control under 40 mM NaCl concentration.
There was no significant difference in the in vitro biomass production between control and treatment with 40 mM NaCl concentration. We measured a significant decrease in dry-matter mass under 120 mM NaCl concentration. There is a need for more investigation of different genotypes and for a conclusion as to whether in vitro tolerance could occur under in vivo circumstances in plants originated from somaclones as well.
Under in vitro conditions, we investigated shoot and leaf callus initiation using different culture media with different 2,4-D concentrations. Under dark conditions, callus induction of shoot/leaf decreased as the 2,4-D concentrations increased.
In light conditions, there was a little callus induction, while callus initiation from the shoot from 5 μM to 12 μM 2,4-D concentration showed a significant increase -
Anaerobe degradation of maize infected by Fusarium graminearum
57-61Views:123Last year intense rainfalls and moisture conditions were beneficial for the Fusarium sp. in Hungary. Fusarium strains decrease cereal quality (for example maize), furthermore may cause yield loss. Due to the toxin production, the fungi have a dangerous animal and human pathogen effect (Placinta et al., 1999).The effects of the Fusarium infection and its mycotoxin production haven’t been perfectly eliminated. Fusariumgraminearum
is the most common agricultural pathogen in Hungary. The utilization of infected maize as an alternative biogas raw material may be an efficient and environmentally friendly disposal method. In this case, Fusarium-, and mycotoxin-content of the maize have to be analyzed as well as the impact of these factors’ on the biogas production process. Our experience was based on the raw material basis of a biogas plant. Different amount of Fusarium free and infected maize grits have been added to the regular raw material mixture. The detection of Fusarium fungi has been analyzed
in experimental digesters throughout the different stages of mesophilic digestion. In the biogas liquid end product the Fusarium was detected by breeding and by microscope. According to our results, the Fusarium sp. was not detectable in the liquid end product after 30 days. -
Comparison of Variability among Irradiated and Control Inbred Maize Lines via Morphological Descriptions and Some Quantitative Features
70-73Views:60Knowledge of genetic diversity in breeding material is fundamental for hybrid selection programs and for germplasm preservation as well. Research has been done with nine irradiated (fast neutron) and four non-treated inbred lines. The aims of this study were (1) to investigate the degree of genetic variability detected with morphological description (based on CPVO TP/2/2) in these materials, (2) to compare the genetic changes among irradiated and non-irradiated maize inbred lines (based on some quantitative features). The irradiation did not change any of the characteristics clearly in positive or negative way, which can be related to the fact that the effect of induced mutation on genetic structure cannot be controlled. From the irradiated lines we have managed to select plants with earlier ripening times and better phenotypes. We could distinguish 3 main groups by the morphological features; these results match our expectations based on pedigree data. Markers distinguishable on the phenotypic level (e.g. antocyanin colouration, length of tassels) were significant in all lines.
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Yearly change of Wheat dwarf virus infection rate during 1996-2010 in winter barley
22-28Views:96Yearly change of the infection of Wheat dwarf virus was studied in winter barley during 1996-2010. Surveys were carried out at Kompolt (Rudolf Fleischmann Research Institute, Róbert Károly College), in winter barley breeding lines showing leaf yellowing and stunting symptoms. In 1996, 250 winter barley samples were tested. During the period of 1997–2005, 100 samples were collected in each year. In 2006, 490 winter barley samples were tested. In 2007 and 2008 the number of samples collected was 500 from winter barley. In 2009 year 100, and in 2010 year 100 winter barley samples were collected for virus testing. Virus diagnosis was carried out using DASELISA for the detection of Wheat dwarf virus (WDV), Barley yellow dwarf viruses (BYDV-MAV, BYDV-PAV, BYDV-RMV, BYDV-SGV), and Cereal yellow dwarf virus (CYDV-RPV). During the ten of the last fifteen years, the occurrence of Wheat dwarf virus in infected samples exceeded those of other viruses causing leaf yellowing and dwarfing symptoms. There were years (1997, 2002, 2004, 2007, 2009 and 2010) when only the Wheat dwarf virus played the main role in development of yirus symtoms. A contrasting tendency can be observed between the degrees of infection of WDV and BYDV. With a rise of infection in the WDV, the proportion of BYDV decreased and vice-versa.
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Agrobotanical Analysis of Bean (Phaseolus vulgaris L.) Equilibrium Populations Suitable for Regional Land Cultivation
148-152Views:55The local bean landraces maintained at small-scale farming level should have importance as complementary source for completion of plant genetic resources with potential role in local land cultivation. Also, they should be used as primary material in breeding activities targeted on improving the foodstuffs quality. In the research were used especially landraces collected in Nyírség, Körös-mellék and Tápióvidék region. Our main objective was to reveal the relationship between the adaptation ability of landraces and the distance from their original collecting place. For that reason the location of the experimental plots was choosen by taking into account that they must be checked in each region, on it’s original region and similarly in other two different regions. On the base of the recorded agro-morphological traits we can get answers on the shaping of adaptation ability, and in the same time by a general chemical characterisation we should know more about the variation of qualitative parameters with a potential economical role. In the designing of experiment a main importance had the conditions where the research is taking place. All activities related to the maintenance of experiment were done in frame of a low input agricultural system to emphasize as much as possible the natural endowments of regeneration places.