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Comparative examination of a mineral fertiliser and a bacterial fertilizer on humic sandy soil
Published December 15, 2010
111-116

In our pot experiment, the impact of a bacterial fertilizer, Bactofil® A10 and a mineral fertilizer Ca(NO3)2 applied in different rates was studied on some soil chemical and microbiological characteristics of a humic sandy soil (Pallag). Perennial rye-grass (Lolium perenne L.) was used as a test-plant. Samples were collec...ted four and eight weeks after sowing in each year. The experiment was set up in 2007-2009 in the greenhouse of
the UD CASE Department of Agrochemistry and Soil Science. The available (AL-extractable) nutrient contents of soil, among the microbial parameters the total number of bacteria, the number of microscopic fungi, cellulose-decomposing and nitrifying bacteria, the sacharase and urease enzyme activity, as well as the soil respiration rate were measured.
Statistical analyses were made by means of the measurements deviation, LSD values at the P=0.05 level and correlation coefficients were calculated. Results of our experiment were summarised as follows:
− The readily available nutrient content of humic sandy soil increased as affected by the treatments, in case of the available (AL-extractable) phosphorus and potassium content the higher value was measured in high-dosage artificial fertilizer treatment.
− The treatments had also positive effect on several soil microbial parameters studied. The higher-dosage mineral fertilizer treatments had a beneficial effect on the total number of bacteria, cellulose-decomposing and nitrifying bacteria. No significant differences were obtained between the effect of treatment in case of the total-number of bacteria, the number of microscopic fungi and nitrifying bacteria.
− On the sacharase enzyme activity the artificial fertiliser treatments proved to be unambiguously stimulating, the urease activity significantly increased on the effect of the lower-dosage Ca(NO3)2 artificial fertilizer treatment. 
− The soil respiration increased in all treatments in related to the amounts applied, significantly increased in the highest rate of Ca(NO3)2 fertilizer addition. 
− Some medium and tight positive correlations were observed between the soil chemical and microbiological parameters studied in case of both nutrient sources. 
Summarizing our results, it was established that the organic and all the mineral fertilizer treatments had beneficial effects on the major soil characteristics from the aspect of nutrient supply. In majority of the examined soil parameters (AL-extractable phosphorus- and potassium, total number of bacteria, number of cellulose-decomposing and nitrifying bacteria, activity of sacharase enzyme) the high rate of Ca(NO3)2 mineral fertilizer treatment proved to be more stimulating, but at the same time the high rate bacterium fertilizer resulted in significant increases in
the nitrate-N content, the AL-potassium content of soil, the total number of bacteria, the number of cellulose-decomposing and nitrifying bacteria and the urease enyme activity. 
Our examinations showed that the mineral fertilizer treatments proved to be more stimulating on most of the soil parameters studied but according to our results, it was established that Bactofil is efficiently applicable in the maintenance of soil fertility and the combined application of
mineral fertilizer and bacterium fertilizer may be a favourable opportunity – also in aspect of the environmental protection – in maintaining soil fertility.

 

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Studies of the influences of different N fertilizers and Microbion UNC bacterial fertilizer on the nutrient content of soil
Published October 5, 2010
134-140

A field experiment was conducted to examine the effects of different nitrogen fertilizers in combination with bacterial fertilizer on
nutrient uptake of horseradish and plant available nutrients of the soil. Three different N fertilizers, ammonium-nitrate, urea and calciumnitrate
(116 kg ha-1 N) in combination with Microbion UNC bacterial... fertilizer (2 kg ha-1) were applied as treatments in a randomized
complete block design in three replications. In this paper we presented the results of soil measurements. The soil of the experimental area
was chernozem with medium sufficiency level of N and P and poor level of K.
Our main results:
The amount of 0.01M CaCl2 soluble inorganic nitrogen fractions, NO3
--N and NH4
+-N and also the quantity of soluble organic-N were
almost the same in the soil. N fertilizers significantly increased all the soluble N fractions. The amount of NO3
--N increased to the greatest
extent and the increase of organic N was the slightest. We measured the largest CaCl2 soluble NO3
- -N and total-N contents in the plots
treated with ammonium-nitrate, the largest NH4
+-N in the plots treated with calcium-nitrate and the largest organic-N fraction in plots
treated with urea.
Bacterial inoculation also increased both soluble inorganic nitrogen forms and also total-N content of soil compared to the control. In
the case of combined (artificial and bacterial fertilizer) treatments we measured lower NO3
--N, organic-N and total-N compared to the
values of plots having only nitrogen fertilizer treatments. On the contrary in the plots with combined treatments the CaCl2 soluble NH4
+-N
content of soil in more cases were higher than that of values with artificial fertilizer treatment.
As a function of calcium-nitrate application increased AL-P2O5 and AL-K2O values were measured compared to control. Microbion
UNC supplement of calcium nitrate yielded also increase in AL-P2O5 and AL-K2O values, till then supplement of ammonium-nitrate fertilizer
yielded a decrease in these values compared to the control.
All nitrogen fertilizers resulted in a significant decrease in AL-Mg content of soil compared to the control. Nevertheless bacterial
fertilizer increased AL-Mg values in any cases.

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9
The effect of different microbial preparations on some soil characteristics
Published July 31, 2012
83-86

In pot experiment the effect of different microbial inoculants and their combinations with NPK fertilizer and wheat straw on some soil properties (physical, chemical, and microbiological parameters) were studied. The experiment was set up in 2011 at the Institute of Agricultural Chemistry and Soil Science, in a three replications, in a random b...lock design. The studied soil type was calcareous chernozem soil from Debrecen (Látókép) with ryegrass (Lolium perenne, L.) test plant.
At the end of the experiment in our laboratory the nitrate-nitrogen content of soil, the AL-soluble phosphorus and potassium content of soil, the urease enzyme activity of soil, the total number of bacteria and the number of microscopical fungi were determined.
The results of the study were the following:
– The straw treatment and the straw + biofertilizer combinations influenced positively the nitrate content of soil.
– The NPK fertilization and the straw+bacterial fertilizer combinations had significant positive effect on the AL-soluble phosphorus content of the soil.
– The biofertilization and the straw+biofertilizer combinations stimulated the AL-soluble potassium content of soil occasionally.
– The total number of bacteria was influenced by the NPK fertilization, the bacterial fertilization and the straw+bacterial fertilizer combinations significantly.
– In case of the number of microscopic fungi caused in some cases significant changes the NPK+bacterial fertilizer and straw+bacterial fertilizer combinations.
– The soil urease enzyme activity was increased in all cases strongly especially by the straw+bacterial preparation combinations.

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The effect of different bacterial fertilizers on the AL-soluble P2O5 content of soil, and the biomass of the rye-grass (Lolium perenne, L)
Published December 16, 2012
93-98

In pot experiment the effect of different bacterial fertilizers on some soil properties, and the amount of plant biomass were studied. The
experiment was set up in 2010 at the Department of Soil Science and Agricultural Chemistry, in a three replications in a random block design. The ryegrass (Lolium perenne, L.) was used as a test plan...t. The studied soil type was calcareous chernosem soil from Látókép. In our laboratory AL-soluble P2O5 content of soil, the phosphatase enzyme activityof soil, the dry weight of rye-grass, and the phosphorus content of rye-grass were determined.
The results of the study were the following:
– The bacterial fertilizers - by basic treatments NPK - had significant positive effect on the AL- soluble phosphorus content of the soil.
– The soil phosphatase enzyme activity was increased in all cases strongly by the microbial preparations used, the greatest impact was the Bactofil A bacterial fertilizer.
– The plant educed P values significantly increased by the effect of microbial products, in addition to the fund NPK. In this case, the EM-1 and Microbion UNC bacterial fertilizer were the effective.
– In case of the rye-grass biomass none of the bacterial preparations used caused any significant changes, either alone or when used them with straw treatment.

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Impact of ammonium nitrate and Microbion UNC bacterial fertilizer on dry matter accumulation of ryegrass (Lolium perenne L.)
Published November 3, 2010
35-39

Pot experiment was performed to investigate the effects of increasing NH4NO3 doses with or without Microbion UNC bacterial fertilizer
application on dry matter production of ryegrass (Lolium perenne L.). Experiment was set up on calcareous chernozem soil of Debrecen-Látókép and on humus sandy soil of Őrbottyán. The bi-factorial trials w...ere arranged in a randomized complete block design with four replications. Grass was cut three times. Dry matter production was determined and the sum of biomass of cuts was calculated as cumulated dry weights. Analysis of variance was carried out on the data in order to provide a statistical comparison between the treatment means. The least significant difference (LSD5%) test was used to detect differences between means. On the basis of our results it can be concluded, that the dry weights of ryegrass cultivated on chernozem soil were higher than on sandy soil. With increasing nitrogen supply the dry matter production of grass significantly increased in both types of soils. In case of sandy soil the increasing effect was more expressed, but dry weights of this soil never reached the appropriate values of chernozem soil. Application of Microbion UNC had positive effect on dry matter production of ryegrass grown on both two types of soils but the effect was more expressed on chernozem soil. Finally it can be concluded that the increasing effect of NH4NO3 on biomass weights was more expressed in both types of soils, the biofertilizer application also increased the dry weights of plant in a small degree. 

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Microbiological preparations affecting the soil nutrient availability and growth of ryegrass in a pot experiment
Published March 11, 2014
49-53

The effects of different bacterial fertilizers and their combinations with NPK fertilizer and wheat straw were investigated on some soil properties (chemical parameters) and on the biomass production of testplant. The applied quantities of the bacterial fertilizers were the double of the recommended dose. The experiment was set up in 2013 at th...e Institute of Agricultural Chemistry and Soil Science, in a three replications, in a random block design. Calcareous chernozem soil; originating from Debrecen (Látókép) was used with ryegrass (Lolium perenne L.) test plant. At the end of the experiment (after 8 week) the samples of soil and plants were determined for nitrate-nitrogen, ALsoluble phosphorus and potassium content of soil, the weight of green biomass of ryegrass per pot, the dry matter and moisture content of ryegrass. Straw treatment resulted better water and available nutrient content of soil in general. Inoculation however was not improving the biomass production over the fertilizer treatment. Interrelation with the recommended dose could be further studied.

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Effects of combined nutrient supply treatments on some physiological parameters of autumn wheat
Published September 5, 2018
241-251

The Fleischmann Rudolf Research Institute in Kompolt is not only famous for plant breeding but the institute also surveys the effects of different nutrient supply methods since 1918. In 2017, we joined this research supported by EFOP 3.6.1 project. Our aim was to investigate photochemical processes – which is one of the most determinant in ca...se of yield – of crops by in vivo field measurements. We measured the chlorophyll content of leaves using Minolta SPAD 502. We used miniPAM fluorometer to determine actual photochemical efficiency and non-photochemical quenching of PSII during natural light conditions and also to evaluate the pigment (chlorophylls and carotenoids) and water content of leaves we applied field spectrophotometer (ASD FieldSpecPro 3). We utilized these methods by various treatments (1. treatment with soil bacteria + head and base fertilizer; 2. treated by only head fertilizer; 3. treated by only base-fertilizer) in field experiment of autumn wheat (4.1–2.43–1.19 ha) in June, 2017. The difference between treatments was clearly detectable. In the case of the first treatment, physiological processes were more intense and the ripening occurred earlier. The obtained yield was the highest in the case of the area treated by soil bacterial. Based on the results, the first treatment can be recommended in practice.

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Studies of the effects of N fertilizers and Microbion UNC biofertilizer on microelement content of horseradish (Armoracia macrocarpa)
Published November 3, 2010
41-45

A field experiment on calcareous chernozem soil was performed to study the effects of different N and bacterial fertilizers on the nutrient content of horseradish (Armoracia macrocarpa). In the experiment the trials were arranged in a randomized block design with three replications, applying three levels of NH4NO3 and diff...erent N fertilizers, namely ammonium-nitrate, urea and calcium-nitrate, with or without application of Microbion UNC biofertilizer.
In the present paper the changes and distribution of manganese, zinc and copper contents of the horseradish plant are summarized by the
effect of different treatments.
The Mn content of leaves were higher in all cases than those of roots, but Zn mainly accumulated in the roots. The distribution of copper within the horseradish plant was more equalized than that of Zn and Mn. Different N fertilizers and increasing doses of ammonium-nitrate had effects mainly on the microelement contents of leaves. The highest Mn contents of plant were measured in treatments of Ca(NO3)2 and Ca(NO3)2+Microbion. The lowest ammonium nitrate dose (N1) decreased the Mn content of leaves compared to control, but further doses
(N2, N3) did not alter these values any longer. Microbion UNC biofertilizer did not have any effect on the Mn content of roots, but we measured higher Mn in leaves in some combined treatments. Ca(NO3)2 increased the zinc content in leaves and roots in a noticable manner. With the increasing of NH4NO3 doses, the Zn content of leaves and roots augmented significantly. Neither N fertilizers (or the increasing doses of NH4NO3) nor the biofertilizer application influenced the Cu content of horseradish plant. 
N fertilizers had higher effects on the microelement content of horseradish, the biofertilizer’s effect was smaller and was not the same in every treatment.

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