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Comparison of RAPD and AFLP Analysis in Some Maize (Zea mays L.) Lines and Hybrids
3-7

The use of molecular markers to enhance plant breeding efforts is being widely studied. DNA-based fingerprinting technologies (RAPD and AFLP) have proven useful in genetic similarity studies. We estimated different maize (Zea mays L.) inbred lines and hybrids originated from mutant ones based on their genetic differences.
We carried out RAPD analysis with different primers and the 707 (CCCAACACCC) and 792 (CAACCCACAC) primers with 50% similarities provided quite good DNA fragments. By applying the DNA based-AFLP technique, we had very dense DNA fingerprinting. We differentiated 15-32 polymorphic bands, the highest number of bands were found in P-T/H-CA (32). AFLP seems to be the more efficient method of comparing genetic similarities/differences among different genotypes.

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Inheritance of Plant and Ear Height in Maize (Zea Mays L.)
34-38

Plant and ear height are very important characters not only for describing new varieties of maize (Zea mays L.), but for green and dry matter production, and even for grain yield. Significant positive correlations have been reported by various authors between plant height and stover yield, plant height and dry matter yield, and plant height and grain yield. The height of the main ear is also correlated to plant height. It depends on the variety or the environment, but is likely to be the same height within a population. Many environmental and agronomical factors (e.g. plant density, fertilization, pests and diseases) influence the expression of these characters, which are not quality traits. Their expression is controlled by many genes and by the interactions between these genes. The heritability of these traits is high and they show significant genotypic variability and positive heterosis, as reported in many research publications.

143
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Determining factors of test weight in maize (Zea mays L.)
40-42

Most domestic maize production products are sold on markets abroad. Among the increasingly restrictive quality requirements, the demand for the measurement of test weight has also appeared. This measurement is not unfamiliar in the case of other cereals, such as wheat and barley, but it has not been applied widely in maize. It is likely for this reason that we have such little information and research available on this topic. In this study, we show the current state of this field with references from domestic and international literature.
The density of maize is the weight of a particular volume and the most frequent unit is the test weight (kg/hl). This physical quality factor plays important roles in the storage, transport and mill industries. The value of test weight is influenced by many factors. The most important ones are the moisture content of grains, drying temperature, drought, precipitation, early frost, and the hybrid characters of a given genotype (grain type, FAO number). In general, the grain with higher moisture content has lower test weight and the higher temperature during (above 82°C) desiccation also leads to unfavourable values. Factors such as a drought interval after flowering, early frost in the case of hybrids with higher FAO numbers, injuries by insects, as well as fungal infections also influence the structure and moisture content of the maize grain.
In the future, broader studies (hybrid testing, application of new agrotechnical elements) will be needed for understanding of the factors effecting test weight.

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Ecotoxicological impact of DON toxin on maize (Zea mays L.) germination
35-40

Fusarium graminearum is one of the most significant arable pathogen in Hungary, and various types of trichothecene mycotoxins (mostly DON, deoxynivalenol) are detected most commonly in cereals (Biró et al., 2011). Fusarium infection and mycotoxin production could not be eliminated, and infected maize by Fusarium sp. cannot be exploited as food, seed, or animal feed. However it can be raw material of biogas production. In this research we would like to investigate the content and effect of the toxin in the end product of biogas production on plant germination. The Fusarium sp. can cause mildew and seedling mortality in seed of maize (Zea mays L.), so we examine the effect of this on germination. In preliminary examination Fusarium sp. was not detected in the bioreactor of the Institute after the retention time (30 day), however it can be assumed that during the hydrolysis of the fungus growth and mycotoxin production also increased exponentially. There were no appropriate tools to detect the toxin in the end product of biogas production so modelling of anaerobic hydrolysis was necessary. The effects of hydrolyzed product for germination were also detected.

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Development of a New Maize (Zea mays L.) Breeding Program
25-30

Genetic manipulation may not replace any conventional method in crop breeding programs, but it can be an important adjunct to them. Plant regeneration via tissue culture is becoming increasingly more common in monocots such as corn (Zea mays L.). In vitro culturability and regeneration ability of corn decreased as homozigosity increased, which suggested that these two attributes were controlled primarily by dominant gene action. Pollen (gametophytic) selection for resistance to aflatoxin in corn can greatly facilitate recurrent selection and screening of germplasm for resistance at a much less cost and shorter time than field testing. Integration of in vivo and in vitro techniques in maize breeding program has been developed to obtain desirable agronomic attributes, speed up the breeding process and enhance the genes responsible for them. The efficiency of anther and tissue cultures in most cereals such as maize and wheat have reached the stage where it can be used in breeding programs to some extent and many new cultivars produced by genetic manipulation have now reached the market.

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Variability examination of photosynthetic pigment content and specific leaf area in individual maize (Zea mays L.) plants
153-157

Currently, maize is one of the most important crops (Zea mays L.) both globally and in Hungary. We compared physiological parameters of a maize genotype – p9903 – at two different experimental sites in a field experiment. Furthermore, we examined these parameters’ variability in individual plants on the leaves with different ages. Absolute chlorophyll content of the leaves were analysed, separately that of chlorophyll a and chlorophyll b. We also measured the absolute carotenoid contents of leaves. Furthermore, we calculated these photosynthetic pigments’ content ratio. Specific leaf area (SLA) and dry matter weight were also measured in order to characterise plant production. The results obviously reflect the decreasing in the efficiency of photosynthetic apparatus on the low yield site. Otherwise, we identify significant differences only in certain cases of leaves.

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Investigation of directions of crossing in maize (Zea mays L.)
43-48

In Hungary, we examined eight features of twelve direct cross hybrids over a period of three years. The twelve hybrids were derived from direct and reciprocal crosses of four parental lines. We did not find significant differences in the cases of stalk diameter and leaf number in the average of 3 years in any of the hybrids. Statistically explainable differences in primary branches were observed in hybrids UDH5 and UDH8 and also in UDH6 and UDH11. It was evident in both instances that degrees of heterosis in reciprocal crosses far exceeded those of direct ones. High tassel branch number was dominant over the low one; consequently, parents with higher tassel numbers enforced their effects during the formation of this trait in hybrids. We experienced positive correlation (r=0.67**) between plant height and main ear height. A positive correlation (r=0.89**) was also found between the tassel length of the main axis above the lowest and the upper side branch. We observed a medium correlation (r=-0.42**) between the number of primary lateral branches and plant height. The number of primary lateral branches showed the highest heterosis. These results can be utilized in practical selection and seed production.

70
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Effects of water deficit on the growth and yield formation of maize (Zea mays L.)
143-148

Maize (Zea mays L.) is the most important consuming cereal crop in the world after rice and wheat. This requires an understanding of various management practices as well as conditions that affect maize crop performance. Water deficit stress during crop production is one of the most serious threats to crop production in most parts of the world and drought stress or water deficit is an inevitable and recurring feature of global agriculture and it is against this background that field study of crops response to water deficit is very important to crop producer and researchers to maximize yield and improve crop production in this era of unpredicted climatic changes the world over.
A pot experiment was carried out to determine the effects of water deficit on growth and yield formation of maize. Two maize cultivars were used Xundan20 and Zhongdan5485. Three levels of soil water content were used in two stages of water control levels at two stages of the maize plant development
1. The JOINTING STAGE: A. CONTROL (CK) soil water content: from 70% to 80% of soil water holding capacity at the field, soil water content: from 55% to 65% of soil water holding capacity at the field, soil water content: from 40% to 50% of the Soil water holding capacity at the field.
2. The BIG FLARE PERIOD: A. CONTROL (CK) soil water content: from 75% to 85% of soil water holding capacity at the field, soil water content: from 58% to 68% of soil water holding capacity at the field, soil water content: from 45% to 55% of the soil water holding capacity at the field.
This research mainly studied the effects of water deficit on physiological, morphology and the agronomical characteristics of the maize plant at the different water stress levels.
The importance of these results in this experiment will enable plant producers to focus and have a fair idea as to which stage of the maize plant’s development that much attention must be given to in terms of water supply.

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Changes of some soil chemical and microbiological characteristics in a long-term fertilization experiment in Hungary
253-265

Agricultural management practices – directly or indirectly – influence soil properties.

Fertilization rates and crop rotation can strongly affect soil pH, soil nutrient supply and soil organic matter content due to the changes of microbial processes. The objective of this study was to compare the effects of different fertilization doses in monoculture and tri-culture of maize (monoculture: only maize grown since 1983, tri-culture: it is a three-year crop rotation system: pea – winter wheat – maize) on selected soil characteristics. The long-term fertilization experiments were set up in 1983 in Eastern Hungary. These experiments are situated west of Debrecen in Hajdúság loess region, on calcareous chernozem (according to WRB: Chernozems).

The test plant was maize (Zea mays L.). One-one pilot blocks were selected from monoculture and tri-culture of the long-term experiments. The observed soil samples were taken in the 30th year of the experiment, in 2013. The doses of NPK fertilizers increased parallel together, so the effects of N-, P- and K-fertilizers cannot be separated.

With the increasing fertilizer doses, the soil pH has decreased in both crop production systems and, in parallel, the hydrolytic acidity has significantly increased. A close negative correlation was proved between the pHH2O, pHKCl and hydrolytic acidity. An increased nutrient content in soil was recorded in every NPK treatment and the available phosphorus and nitrate content increased in higher proportion than that of potassium. Of the measured parameters of C-and N-cycles, fertilization has mostly had a positive effect on the microbial activity of soils. Besides the effects of fertilizer doses, correlation were looked for between soil microbiological properties. Evaluating the ratios among the measured parameters (organic carbon and microbial biomass carbon, OC/MBC ratio; carbon-dioxide and microbial biomass carbon; CO2/MBC proportion), the fertilization rate seems to be favoured by the increase of amounts of organic compounds

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Examination of Zn deficiency on some physiological parameters in case of maize and cucumber seedlings
5-9

Zinc (Zn) is an essential micronutrient needed not only for people, but also crops. Almost half of the world’s cereal crops are deficient in
Zn, leading to poor crop yields. In fact, one-third (33%) of the world's population is at risk of Zn deficiency in rates, ranging from 4% to
73% depending on the given country. Zn deficiency in agricultural soils is also a major global problem affecting both crop yield and quality.
The Zn contents of soils in Hungary are medium or rather small. Generally, the rate of Zn deficiency is higher on sand, sandy loam or soil
types of large organic matter contents. High pH and calcium carbonate contents are the main reasons for the low availability of Zn for
plants (Karimian and Moafpouryan, 1999). It has been reported that the high-concentration application of phosphate fertilisers reduces Zn
availability (Khosgoftarmanesh et al., 2006). Areas with Zn deficiency are particularly extensive in Békés, Fejér and Tolna County in
Hungary, yet these areas feature topsoils of high organic matter contents. Usually, Zn is absorbed strongly in the upper part the soil, and it
has been observed that the uptakeable Zn contents of soil are lower than 1.4 mg kg-1.
Maize is one of the most important crops in Hungary, grown in the largest areas, and belongs to the most sensitive cultures to Zn
deficiency. Zn deficiency can causes serious damage in yield (as large as 80 %), especially in case of maize. On the other hand, Zn
deficiency can also cause serious reduction in the yields of dicots. One of the most important vegetables of canning industry is cucumber,
which is grown all over the world.
In this study, the effects of Zn deficiency have investigated on the growth of shoots and roots, relative and absolute chlorophyll contents,
fresh and dry matter accumulation, total root and shoot lengths, the leaf number and leaf area of test plants in laboratory. Experimental
plants used have been maize (Zea mays L. cv. Reseda sc.) and cucumber (Cucumis sativus L. cv. Delicatess). A monocot and dicot plant have
chosen a to investigate the effects of Zn deficiency, because they have different nutrient uptake mechanism.
It has been observed that the unfavourable effects of Zn deficiency have caused damage in some physiological parameters, and
significantly reduced the growth, chlorophyll contents of monocots and dicots alike.

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Effects of the cropyear and the agronomical factors on agronomical elements of different sweet corn (Zea Mays L. convar. saccharata Koern.) genotypes in long-term experiment
105-110

In the crop season of 2010 (rainy year), we studied the effect of three agrotechnical factors (sowing time, fertilization, plant density) and four different genotypes on the agronomical characteristics of sweet corn on chernozem soil in the Hajdúság. The experiments were carried out at the Látókép Experimental Farm of the University of Debrecen. In the experiment, two sowing dates (27 April, 26 May), six fertilization levels (control, N30+PK, N60+PK, N90+PK, N120+PK, N150+PK) and four genotypes (Jumbo, Enterprise, Prelude, Box-R) were used at two plant densities (45 thousand plants ha-1, 65 thousand plants ha-1). The amount of precipitation in the season of 2010 was 184 mm higher, while the average temperature was 0.8 oC higher in the studied months than the average of 30 years. Weather was more favourable for sweet maize at the first sowing date, if we consider the yields, however, if we evaluate the agronomical data and yield elements (number of cobs, cob length and diameter, the number of kernel rows, the number of kernels per row) it can be stated that the size of the fertile cobs was greater at the second sowing date due to the lower number of cobs. The largest number of fertile cobs was harvested in the case of the hybrid Enterprise (72367.9 ha-1) in the higher plant density treatment (65 thousand ha-1) at the fertilization level of N120+PK when the first sowing date was applied. The largest cobs were harvested from the hybrid Box-R (cob weight with husks: 516.7 g, number of kernels in one row: 45.7) at the lower plant density (45 thousand plants ha-1) in the second sowing date treatment. Cob diameter and the number of kernel rows were the highest for the hybrid Prelude.

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The effect of sowing date and plant density on the yield of maize (Zea mays L.) under different weather conditions
205-208

Maize has high productivity and produces huge vegetative and generative phytomass, but this crop is very sensitive to agroecological (mainly to climatic, partly to pedological conditions) and agrotechnical circumstances. In Hungary, maize is grown on 1.1–1.2 million hectares, the national average yields vary between 4–7 t ha-1 depending on the year and the intensity of production technology. The longterm experiment was set up in 2015–2016 on chernozem soil in the Hajdúság (eastern Hungary). The maize research was set up on chernozem soil at the Látókép MÉK (Faculty of Agricultural and Food Sciences and Environmental Management) research area of the University of Debrecen. We examined the following commonly used hybrids of Hungary: SY ARIOSO (FAO 300), P9074 (FAO 310), P9486 (FAO 360), SY Octavius (FAO 400), GK Kenéz (FAO 410), DKC 4943 (FAO 410). The experiment was set up in three different plant densities. These were 60, 76, 90 thousand plant ha-1. The experiment was set up with three different sowing dates, early, average and late sowing. The yield was measured using a special plot harvester (Sampo Rosenlew 2010), measuring the weight of the harvested plot and also taking a sample from it. As a next step, we calculated the yield (t ha-1) of each plot at 14% of moisture content to compare them to each other. We evaluated the obtained data using Microsoft Excel 2015.

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The effect of hybrid, nutrient-supply and irrigation on the grain moisture content at harvest and the starch-content of maize (Zea mays L.)
89-95

Maize is a worldwide dominant plant. According to nowadays plant production principles it is important to investigate and optimize the site-specific nutrient-supply and other production factors, such as hybrid and irrigation, in the case of this plant as well.
At the Research Institute of the University of Debrecen, Center of Agricultural Sciences and Engineering, at Látókép the effect of nutrient-supply and irrigation on the quantity and quality parameters of different hybrids were investigated in a small plot long-term field experiment. In this paper we introduce the results regarding the corn moisture-content and the starch content of the yield.
We have chosen three maize hybrids – that have been bread in Martonvásár – for our investigations. The effect of macronutrients is investigated in this experiment on five levels. The half of the experimental area can be irrigated during the vegetation period – whenever it is needed – by linear irrigation equipment, but on the other half only the water amount originating from the precipitation can be used by plants.
In the year 2008 the hybrid affected the grain moisture content at P=0.1% level, while nutrient-supply had an effect at P=10% significance level. We haven’t revealed either any effect of irrigation or of interrelationship between production factors. It can be stated that there are differences between the hybrids on each nutrient-supply and on both irrigation levels. The grain moisture content increased parallel to the longer vegetation
periods.
The starch content of maize is mostly affected by the hybrid,
so on P=0.1% significance level. Regarding our results, it can be
stated, that the starch content shows a decreasing tendency
parallel to the longer vegetation periods.

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Effect and interaction of crop management factors and crop year on the yield of maize (Zea mays L.)
31-41

The aim of this study was to determine the combination of treatment levels of crop management factors which can optimize and sustain maize yield under varying climatic conditions. The effect of winter wheat forecrop, three tillage systems (Mouldboard-MT, Strip-ST, Ripper-RT), two planting densities (60,000 & 80,000 plants ha-1), three fertilizer levels (N0-control, N80, N160 kg ha-1) with four replications in irrigated and non-irrigated treatments were evaluated over a five year period, 2015–2019. The obtained results revealed that growing season rainfall positively correlated with yield, whereas, temperature negatively correlated with yield. Impact of adverse weather on yield was less severe in biculture, irrigated plots, at lower planting density (60,000), lower fertilizer rate (N80) and in RT and ST, compared to MT. In years with favorable rainfall, yields of MT and RT were significantly (P<0.05) higher than ST. However, in a less favorable year, such as 2015, with 299 mm growing season rainfall and the lowest July rainfall (59% below mean) there was no significant difference (P>0.05) in yield among the three tillage treatments. Higher planting density (80,000), and fertilization rate (N160) in tandem with MT are treatments combination conducive for high yield under favorable climatic conditions, whereas, in years with low rainfall and high temperatures, RT and ST offer alternative to MT for optimum yield with 60,000 plants ha-1 and N80 treatment level. Crop year effect accounted for 20.7% of yield variance, fertilization 35.8%, forecrop 12.8%, plant density 3.4%, tillage 1.2% and irrigation <1%. It is conclusive that with proper selection of the appropriate levels of agrotechnological inputs the adverse effect of weather on yield can be mitigated.

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Effect of the plant density on different maize (Zea mays L.) hybrids yields and leaf area index (LAI) values
51-56

We have investigated the plant number reactions of three maize hybrids of various genotypes in a small-plot field experiment. The plant numbers were 50, 70 and 90 thousand ha-1, while the row distances were 45 and 76 cm. The experiment was set on the Látókép Experimental Farm of Centre for Agricultural Sciences of the University of Debrecen in four replications on calcareous chernozem soil.

The assimilation area and the leaf area index have important role in development of the crop yield. The studied three different genotype maize hybrids reached its maximum leaf area index at flowering. The maximum leaf area index increased linearly with increasing plant density. The season-hybrids reached less yield and leaf area index. According to our experimental results, we have concluded that with the decrease of the row spacing, the yield increased in the average of the hybrids. The studied hybrids reached the maximum yield at 70 and 90 plants ha-1 plant density. We determined the optimal plant number that is the most favourable for the certain hybrid under the given conditions.The higher plant density was favourable at 45 cm row spacing than 76 cm. The hybrids reached the maximum grain yield at 45 cm row spacing between 76 712–84 938 plants ha-1, while the optimum plant density at 76 cm row spacing changed between 61 875–65 876 plants ha-1.

The leaf area index values between the applied plant density for the flowering period (July 1, 24), we defined a significant differences. In the archived yields were significant differences at the 45 cm row spacing between 50 and 70, 90 thousand ha-1 plant density, while the number for the 76 cm row spacing used did not cause a significant differences in the yield. There were significant differences between the examined hybrids of yields.

129
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The effect of sowing time on the yield and the variance of the seed moisture content a harvest of maize (Zea mays L.) hybrids
39-49

Sowing time is an important crop technology element of maize. We studied the effect of this factor on the growth and production of maize in an experiment carried out near Hajdúböszörmény, in 2003 and 2004, and near Debrecen, in 2005.
The soils of the experiments were humic gley soil and chernozem. Weather in both years differed greatly. 2003 was drought. Neither the distribution, nor the quantity of the precipitation were suitable in the growing season for maize. This fact basically determined the results.
In 2004 and in 2005, there were favorable and rainy seasons. The distribution and quantity of precipitation were suitable between April and September. The average temperature was also suitable for maize.
In 2003, we tested seven hybrids at four sowing times. Hybrids with a shorter vegetation period gave the highest yield at the later sowing time, while the hybrids with a longer vegetation period gave them at the earlier sowing time. The yield of PR34B97, PR36N70, PR36M53 hybrids were the best at every sowing times. The moisture loss of hybrids in the late maturity group was faster in the maturity season, but the seed moisture content was higher than the hybrids with early sowing time. The seed moisture content was very low due to the droughty year. In two hybrid cases, this value was higher than 20% only at the fourth sowing time.
In 2004, we examined the yield and seed moisture contents of nine hybrids. In the favorable crop year, the yield of every hybrid was the highest at the second and third sowing times. Yields of PR34H31 and PR38B85 hybrids were significant. The seed moisture content at harvest was higher than the previous year, due to the rainy season. In the case of hybrids sown later, this value was higher by 30%. However, we noticed that this value was lower at the earlier sowing time, than at the later.
In 2005, we applied three sowing times. Unfortunately, the results of the third sowing time could not be analyzed, due to the low plant density. The yield of the six hybrids varied from 12 to 14 t/ha at the first sowing time. At the second sowing time, the yields fluctuated and each hybrid had the lowest yield, except the PR37D25 hybrid. At the latest sowing time, the yield of the PR34B97 hybrid was the lowest. However, this low yield was due to damage from the Western corn rootworm (Diabrotica virgifera) imago. The moisture content at harvest of the hybrids varied from 16 to 24% at the first sowing time. Yields at the second sowing time were higher. The low yield of the PR34B97 hybrid coupled with a higher seed moisture content. In addition, the maximum value of the LAI was more favourable at the first sowing time, and ranged between 5-5.5 m2/m2.
The crop year had a more dynamic effect on maize than the sowing time. First of all, the quantity and distribution of precipitation played an important role in respect to yield safety.

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Challenges and limtations of site specific crop production applications of wheat and maize
101-104

The development and implementation of precision agriculture or site-specific farming has been made possible by combining the Global Positioning System (GPS) and the Geographic Information Systems (GIS). Site specific agronomic applications are of high importance concerning the efficiency of management in crop production as well as the protection and maintenance of environment and nature. Precision crop production management techniques were applied at four locations to evaluate their impact on small plot units sown by wheat (Triticum aestivum L.) and maize (Zea mays L.) in a Hungarian national case study. The results obtained suggest the applicability of the site specific management techniques, however the crops studied responded in a different way concerning the impact of applications. Maize had a stronger response regarding grain yield and weed canopy. Wheat was responding better than maize concerning plant density and protein content performance.

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Az NPK-trágyázás hatása a kukorica tápelemfelvételének dinamikájára, öntözött és nem öntözött viszonyok között
23-27

The effect of NPK-fertilization on the dynamics of nutrient uptake of maize (Zea mays L., cv. Clarica) was examined on chernozem soil under irrigated and non-irrigated conditions in a field experiment.
The following results were made:
• the element concentrations in the plant decreased over time,
• there is no difference between the dynamics of nutrients on irrigated and non-irrigated sites because rainfall was satisfactory for plants in vegetation period,
• the N doses not only significantly increase the nitrogen content in maize, but also have a noticable effect on Ca and Mg concentrations,
• because of the acidifying effect of N-fertilizers, increasing the amount of N-fertilizer increased the Mn, Zn, Cu content of the plants,
• the P doses have a significant effect on the maize P and N content and the Zn concentration of the plant via P-Zn antagonism in the soil,
• as the high K doses treatments alter the ion ratios in the soil, the Ca, Mg content of the plant decreased.

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Environmental friendly maize (Zea mays L.) production on chernozem soil in Hungary
133-135

We have been studied the effects of crop-rotation, fertilization and irrigation on the yields of maize in different cropyears characterized
by different water supply (2007 year=dry; 2008 year=optimum) on chernozem soil. Our scientific results proved that in water stress
cropyear (2007) the maximum yields of maize were 4316 kg ha-1 (monoculture), 7706 kg ha-1 (biculture), 7998 kg ha-1 (triculture) in non
irrigated circumstances and 8586 kg ha-1, 10 970 kg ha-1, 10 679 kg ha-1 in irrigated treatment, respectively. In dry cropyear (2007) the
yield-surpluses of irrigation were 4270 kg ha-1 (mono), 3264 kg ha-1 (bi), 2681 kg ha-1 (tri), respectively. In optimum water supply cropyear
(2008) the maximum yields of maize were 13 729-13 787 (mono), 14 137-14 152 kg ha-1 (bi), 13 987-14 180 kg ha-1 (tri) so there was no
crop-rotation effect. In water stress cropyear (2007) fertilization caused yield depression in non irrigated treatment (control=2685 kg ha-1;
N240+PK=2487 kg ha-1). Our scientific results proved that the effects of abiotic stress could be strongly reduced by using the optimum crop
models in maize production. We obtained 8,6-11,0 t ha-1 maximum yields of maize in water stress cropyear and 13,7-14,2 t ha-1 in optimum
cropyear on chernozem soil with using appropriate agrotechnical elements.

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Effect of tillage practices, fertilizer treatments and crop rotation on yield of maize (Zea mays L.) hybrids
43-48

This research was conducted at the University of Debrecen Látókép Research Station and is part of an ongoing long-term polyfactorial experiment. The impact of three tillage systems (Mouldboard plowing-MT, Strip tillage-ST, Ripper tillage-RT) and two levels of fertilizer treatments (N80 kg ha-1, N160 kg ha-1) along with a control (N0 kg ha-1) on the yield of maize hybrids (Armagnac- FAO 490 & Loupiac-FAO 380) cultivated in rotation with winter wheat was evaluated during a two-year period (2017–2018).

Amongst the three tillage treatments evaluated, ripper tillage (RT) had the highest average yield (10.14 t ha-1) followed by mouldboard tillage (MT) and strip tillage (ST) with 9.84 and 9.21 t ha-1 respectively. Yield difference between RT and MT was not significant (P>0.05), as compared to ST (P<0.05). Soil moisture content varied significantly with tillage practices and was highest in ST, followed by RT and MT (ST>RT>MT). Yield of RT was 7–9% higher than MT in monoculture plots, while MT reign superior in biculture plots (monoculture: RT>MT>ST; biculture: MT>RT>ST).

A positive interaction between tillage and fertilization was observed, with higher yield variation (CV=40.70) in the non-fertilized (N0) plots, compared to those which received the N80 (CV=19.50) and N160 kg ha-1 (CV=11.59) treatments.

Incremental yield gain from increase fertilizer dosages was significantly higher in monoculture, compared to biculture. There was no significant difference in yield between N160 and N80 in the biculture plots (12.29 vs 12.02 t ha-1). However, in monoculture plots, N160 yield was 23% higher than the N80 kg ha-1 (N160=11.74 vs N80=9.56 t ha-1).

Mean yield of maize in rotation with winter wheat was 28% (2.47 tons) higher than monoculture maize. The greatest benefit of crop rotation was observed in the control plots (N0) with an incremental yield gain of 4.39 tons ha-1 over monculture maize (9.92 vs 5.43 t ha-1).

Yield increased with higher fertilizer dosages in irrigated plots. Fertilizer application greatly increased the yield of maize and accounted for 48.9% of yield variances. The highest yield (11.92 t ha-1) was obtained with N160 kg ha-1 treatment, followed by N80 kg ha-1 (10.38 t ha-1) and N0 kg ha-1 (6.89 t ha-1) respectively.

Overall mean yield difference between the two hybrids was not statistically significant, however, yield of FAO 380 was 3.9% higher (9.06 vs. 8.72 t ha-1) than FAO 490 in monoculture plots, while in biculture plots, FAO 490 was 4.1% higher than FAO 380.

Average yield in 2018 was 13.6% (1.24 t ha-1) higher than 2017 for the same set of agrotechnical inputs, thus, highlighting the significant effect of cropyear.

Armagnac (FAO 490) cultivated in rotation with winter wheat, under ripper tillage and N80 kg ha-1 is the best combination of treatments for optimum yield.

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Analysis of maize and sunflower plants treated by molybdenum in rhizobox experiment
11-14

In this study, maize (Zea mays L. cv. Norma SC) and sunflower (Helianthus annuus L. cv Arena PR) seedlings treated by molybdenum (Mo) that were cultivated in special plant growth boxes, known as rhizoboxes. During our research we tried to examine whether increasing molybdenum (Mo) concentration effects on the dry mass and absorption of some elements (molybdenum, iron, sulphur) of shoots and roots of experimental plants.

In this experiment calcareous chernozem soil was used and Mo was supplemented into the soil as ammonium molybdate [(NH4)6Mo7O24.4H2O] in four different concentrations as follow: 0 (control), 30, 90 and 270 mg kg-1.

In this study we found that molybdenum in small amount (30 mg kg-1) affected positively on growth of maize and sunflower seedlings, however, further increase of Mo content reduced the dry weights of shoots and roots. In case of maize the highest Mo treatment (270 mg kg-1) and in case of sunflower 90 mg kg-1 treatment caused a significant reduction in plant growth.

In addition, we observed that molybdenum levels in seedling were significantly elevated with increasing the concentration of molybdenum treatment in comparison with control but the applied molybdenum treatments did not affect iron and sulphur concentration in all cases significantly.

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Investigation of genetic diversity in irradiated maize lines and its relation to hybrid performance
20-26

Knowledge of genetic diversity among available parental lines is fundamental for successful hybrid maize breeding. The aims of this study were to estimate (1) genetic similarity (GS) and genetic distance (GD) (based on Jaccard index) in four maize inbreed lines; (2) to classify the lines according to their GD and GS; (3) to determine hybrid performance based on GD and heterosis for yield ability in 4x4 full diallel system. We used morphological description and AFLP (amplified fragment length polymorphisms) for estimation genetic polymorphism in four maize inbred lines. We estimated the applicability of genetic similarity in SC and reciproc hybrids for prediction of their performance.
Three primer combinations were used to obtain AFLP markers, producing 207 bands, 70 of whit were polimorphic. The dendogram based on genetic similarities (GS) and genetic distance (GD) and morphological description separated four inbred lines into well-defined groups. Morphological description just with AFLP analysis showed reliable results. In view of genetic distance, the UDL 1 line and their linear and reciprocal crosses showed significant heterosis effect, which was confirmed by heterosis calculation based on grain yield.

109
124
Results of the sensory analysis of precision maize production
59-62

This research was carried out in 2018, at the Látókép Experimental Station of the University of Debrecen in a moderately warm and dry production area, on deep humus layered medium-hard calcareous chernozem soil. In the scope of the research, the chlorophyll content of maize (Zea mays L.) was examined under field circumstances by means of local sensory measurements and we were looking for correlation between the obtained values and the amount of yield. Our measurements were carried out with Minolta SPAD-502 and GreenSeeker devices at 3 measurement times (4 leaf stage, 10 leaf stage and silking). It was found that phenological phases had an effect on the obtained SPAD and NDVI values and were in a slightly significant correlation with the yield. The most significant correlation was found between the results obtained during silking and the amount of yield. This may be because the least time has passed between the measurement time and harvest. Results obtained during the 10-leaf stage show excessive values in each case, which can be due to a measurement error. It was found that the phenological phase had an effect on the correlation of SPAD and NDVI values and the amount of yield. As the phenological phase progressed, the correlation between the measured results and yield has increased.

146
381
Investigation of the Effect of P-Supply on Maize (Zea mays L.) Development
171-173

Quantification of plant development is important in areas where the actual dates of certain phonological stages should be identified. The influences of different ecological factors are put into algorithms by crop modellers. In this process the effect of plant nutrients are not yet well quantified. This paper gives data on the effect of phosphorus on maize development prior to flowering stage. The main conclusions are as follows: 1. In lack of phosphorus more degree days are needed for flowering then in case of well P-supply. 2. The effect of P is seen not on leaf initialisation (i.e. final eaf number is the same) but rather on the phyllochron interval. 3. P effect on phyllochron interval can be observed at the younger stage (prior to leaf number 10) but not later.

53
26
Establishing biotic stress tolerance of maize (Zea mays L.) by measuring hydroxamic acid contents
107-112

 

Cyclic hydroxamic acids are the most considerable secondary metabolites in grasses and their main task is to protect these species from pathogens and pests. The cyclic hydroxamic acid content and common smut susceptibility were examined in our experiments. 27 maize hybrids were used for experimental plants in a climate room, where the plants were grown on a nutrient solution. An infiltration method was used for the inoculation of the plants. The total quantity of cyclic hydroxamic acids was determined and the ratio of infected plants and the ratio of inhibition was determined, too. Based on our results, on the basis of all hybrids’ data, the total hydroxamic acid content of the infected plants was higher than in the control. On the level of individual hybrids, only 9 of them had higher cyclic hydroxamic acid content in the case of infection. Increase in cyclic hydroxamic content induced by the fungus in this case is a tool for the fungus to suppress other pathogens and pests. Amongst the hybrids’ cyclic hydroxamic acid contents, significant differences were detected in the control and in the infected treatment, too. The so-called “sweetcorn” hybrids showed high level of cyclic hydroxamic acid content. According to the differences amongst hybrids, homogenous groups were created which groups differed in the case of control and infected treatment, because of the difference in increase of cyclic hydroxamic acid content. The examined hybrids showed different levels of infection and different rate of growth inhibition for the effect of inoculation. According to the infection caused damage hybrids were ranked. Infection caused notable damage for hybrids Prelude, Desszert 73, DKC5276 and DK440.

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