The aim of the present study was to determine marker proteins those are associated with functional longevity of dairy cattle. Holstein-Friesian cows were grouped based on their performance as follows: group 1) individuals with good longevity traits; group 2) short production life because of poor reproduction traits; group 3) short production li
...fe with low milk yield. Twelve individuals were sampled in each group, blood and milk samples were collected from cows. Blood samples were analysed with two dimensional polyacrylamide gel electrophoresis (2D PAGE), MALDI TOF/TOF and nanoLCMS/MS. The milk samples were analysed with MALDI TOF/TOF and nanoLC-MS/MS. Using the optimized gel based proteomic approach, we have succesfully separated 143 proteins in the group1, 139 proteins in the group2 and 136 proteins in the group3, but we could not find significant differences between groups in the expression pattern. Using MALDI TOF/TOF and nanoLC-IonTrap MS, we have found eleven protein sequences those were expressed only in the samples of good longevity group.
Recently, test day models (TDM) began to be increasingly used for the genetic evaluation of dairy cattle. The main advantage of the TDM compared with the 305 days lactation yield models is that more effects can be used in the evaluation. Therefore, the TDM is more accurate than the lactation models. The main disadvantage is the increased comput
...ational requirement, but this can be offset by improvements in computer capabilities. The topic of this paper is the use of a fix regression test day model to estimate the inheritabilities of test day data from Hungarian Holstein-Friesian dairy cattle. The inheritability was 0.26 for milk production, 0.2 for fat production, 0.24 for protein production and 0.06 for the somatic cell count.