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Experiments for Isolating and Culturing Soil-borne Mycobacteria (Contemporary Publication)
107-110Views:57On grounds of the several thousand tests performed in the field of this topic, the following conclusions may be arrived at:
1. The informations available and the experimental data on soil mycobacteria are very incomplete.
2. Of the 77 strains isolated from similar soil types so far, and adaptable for pure basic culture, 47 strains are confusingly similar, from morphological aspects, to the mycobacteria isolated from clinical material.
3. The apparently homogeneous cultures isolated from the soil are generally co-infected and, therefore, the morphological, biochemical, and other physiological characteristics of the isolated strains can be studied only on base cultures after purification.
4. For the isolation of the soil mycobacteria experiments qualified hitherto as most suitable processes the 4 or 1 per cent NaOH neutralized with H2SO4, and the 1 per cent NaOH or 1 per cent Na3PO4 treatments, on Gottsacker agar medium with plate or top pouring, at a temperature of 29 to 37 C°, in a soil suspension sequence of 1:500 to 1:5000 final dilution.
5. The Ziehl-Nielsen staining of the isolated mycobacteria composed to sub-cultures is best performed by heating with an infra red radiator from above, instead of the gas flame used so far to heat from below.
The repetition of the biochemical test of the hitherto isolated 77 purified strains is under progress, and will be reported on in our next scientific publication. -
The effect of agrotechnological factors and the cropping season on sweetcorn (Zea Mays L. convar. saccharata Koern.) production in a humid year
146-151Views:70We have examined the effect of three agrotechnological factors (sowing time, fertilization, crop density) and four genotypes on the yield
of sweetcorn on chernozem soil in the Hajdúság region in 2009. The experiment was set up at the Látókép Research Site of the University of
Debrecen. We have included two sowing times (27 April, 26 May), six nutrition levels (control, N30+PK, N60+PK, N90+PK, N120+PK,
N150+PK) and four genotypes (Jumbo, Enterprise, Prelude, Box-R) at two crop density levels (45 thousand ha-1).
In the humid cropyear of 2010 the amount of precipitation exceeded the 30-year average by 184 mm in the cropping season; the average
temperature exceeded the same by 0.8 C on the average of the examined months. The circumstances were most favourable for sweetcorn
production with the first sowing time, thus, this was when the yield of all hybrids was the highest.
With early sowing time, the highest yield (23437 kg ha-1 yield) was obtained with Enterprise at 45 thousand ha-1 crop density level at
N150 + PK nutrition level. The highest yield of the other three hybrids was 22253 kg ha-1 (Jumbo) 22286 kg ha-1 (Box-R) and 1873 kg ha-1
(Prelude). With the second sowing time, the highest yield was obtained with Enterprise again (22237 kg ha-1) at 65 thousand ha-1 crop
density level. With this sowing time the yield of Jumbo, Box-R and Prelude was 20888 kg ha-1, 17796 kg ha-1 and 17401 kg ha-1, respectively.
We found that the highest yield was obtained at the highest nutrition levels (N120 + PK, N150 +PK) with the first sowing time, while the same
was obtained at lower nutrition levels (N90 + PK, N120 + PK) with the second sowing time. -
Evaluation of decreasing moisture content of different maize genotypes
147-151Views:222An experiment was conducted to evaluate the decrease in grain moisture content in three maize hybrids in Debrecen in 2017. Armagnac, Sushi and Loupiac were the examined hybrids in this study. The culture medium and temperature conditions were applied uniformly for all three hybrids. According to the results obtained from the ratio of moisture content of seeds per day, the Armagnac variety in the intensive drying down phase loses more time and moisture content, so it can be concluded that the produced dry matter is more than in the case of other varieties. Armagnac requires more time to achieve yield, while the Sushi and Loupiac hybrids produce less harvest. Regarding the slope of the regression line, the rate of loss of moisture in the grain has been negatively correlated with the amount of “b” in the three examined hybrids. In regression analysis, the coefficient of explanation showed that the effect of day in the Armagnac was 97% in the Loupiac, 95% and in the Sushi 90% of the total dynamic value of moisture motion.
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The effect of season on the microbiological status of raw milk
95-99Views:117Many factors can influence the microbiological quality of raw cow’s milk. In this study, our aim was to determine whether there was any difference between the microbiological statuses of milk produced in different seasons. Samples were collected and analysed from five dairy farms in Hajdú-Bihar County, from February to November in 2019. During our studies, total plate count (TPC), coliform count and Staphylococcus aureus count of raw cow’s milk samples were determined.
There was no significant difference (P>0.05) between the mean TPC values detected in the milk collected in winter and autumn, but that values were significantly (P<0.05) lower than in the milk samples collected in spring and summer. Similarly to the TPC, in the case of coliform bacteria the lowest mean colony count was detected in the samples collected in winter. The difference was significant (P<0.05), compared to the values observed in the samples collected in summer. S. aureus was detected in bulk milk of only two farms in excess of 1.0 log10 cfu/ml. Also in case of S. aureus, there was a significant difference (P<0.05) between the values observed in the samples collected in winter and in summer. Samples from spring and summer contained the highest amount of S. aureus.
Based on the results of our studies, in the case of almost all farms the mean TPC, coliform and S. aureus counts were lower in the samples collected in winter, than in the samples collected in summer. The fact that the samples collected in winter contained the lowest amount of colonies could be attributed to the inhibition of growth of mesophilic microorganisms below 8 °C. Furthermore, the fact that we observed the highest colony counts in samples collected in summer, can be related to the heat stress of cows during the summer due to unfavorable weather conditions (high temperature and humidity).
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Development of technology elements for growing of perennial sorghum
15-17Views:122Optimal sowing time for perennial sorghum under irrigation is when soil temperature at the depth of sowing reaches up to 10–11 °С, harvesting for the green mass has to be done when panicle is situated on the stem in 10 sm from the flag leaf and hight of cut must be 11 sm.
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Heat stress of cattle from embryonic phase until culling
11-22Views:246Heat stress becomes a serious problem in the livestock sector as it affects cows' performance negatively. The objective of this paper review is to investigate the effects of heat stress during the different phases of the life cycle of cows; embryos, calves, heifers, and cows. Heat stress during early maternal gestation affects the ability of embryos to develop increasing the risk of abortion and early embryonic death. Heat stress during late maternal gestation affects the performance of calves and heifers later in their life, as it reduces growth performance, conducts physiological changes, impaired immunity, changes the behavior, and reduces the length and intensity of the estrus in heifers with decreasing in milk production in the first lactation. On the level of cows, milk quality and production, meat quality, and the final body weight decrease under hot temperatures. Heat stress decreases the conception rate, alters follicle growth, and estrous symptoms. Hormones secretion and physiological changes because of the heat stress conduct to impair the immunity system, and in oxidative stress and death in some cases. Same as for calves and heifers a change in the behavior of cows was detected in order to decrease their temperature.
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L’incubation et l’éclosion des oeufs des vers à soie de chêne Antheraea pernyi Guer (Bombycidae)
61-63Views:55In this paper we studied optimal conditions which need to be fulfilled in order to have successful incubation and hatching of eggs of the species Antheraea pernyi Guer.
The incubation of the eggs of this species implies a proper environment: temperature of 18-21oC, relative humidity of 80%, unvarying light, aeration 2 or 3 times a day and a density of 50 g eggs for a 20/50 cm area.
In these conditions, the incubation of the first generation lasts 10-12 days and of the second generation up to 8-10 days. The second generation needs higher temperatures (22-26oC) and higher humidity (optimal value 90%).
In this species, hatching lasts 6-7 days, with a maximum during the second day; it takes place especially early in the morning. -
Effect of two different cropyear and the agrotechnological factors on the agronomic characteristic of the winter weat in a lon-term experiment
143-149Views:107We studied the effects of crop rotation, fertilization and crop protection technologies on occurence of the major ear- and leaf-diseases (powdery mildew, helminthosporiosis, leaf rust, Fusarium wilt) and the degree of lodging in the winter wheat variety Mv Pálma in two very different years (2006/2007=dry; 2009/2010=rainy). The experiments were carried out at the Látókép Experimental Farm of the University of Debrecen CAAES in triculture (pea-wheat-maize) and biculture (wheat-maize) at five fertilization levels by applying three different crop protection technologies (extensive, average, intensive).
In the cropyear of 2006/2007, the disease severity of leaf diseases was higher than the average in both crop rotations in spite of the fact that the weather during the whole vegetation period was dry. Infection by powdery mildew, helminthosporiosis and leaf rust increased with increasing fertilization, the highest infection was measured at the highest fertilization level (N200+PK) in the extensive crop protection technology. According to the results, no infection of ears by Fusarium and no lodging occurred in either bi- or triculture due to the dry year. The extremely rainy weather in 2009/2010 was favourable not only for the vegetative development of the stand, but also increased the occurance of leaf-, stalk- and ear-diseases and a high degree of lodging was observed. The highest infection by powdery mildew was observed in the plots with the highest fertilizer dosage under an extensive crop protection technology. A much higher helminthosporiosis infection was measured than in the cropyear of 2006/2007. The wet weather and higher than average temperature promoted the occurence and spreading of leaf rust. Under an extensive crop protection technology, a leaf rust infection of 24% and 31% was detected after maize and pea as a forecrop, respectively, in the N200+PK treatment. As opposed to 2006/2007, the disease severity of Fusarium was 3–8% and 2–7% in the control after maize and pea as a forecrop, respectively. This value, similarly to that of other pathogens, increased with increasing fertilization levels. Due to the large vegetative mass, a significant lodging was observed in the wheat stands in both bi- and triculture (17–100%, 12–100%). -
Effect of cropping technologies on the yield of dry bean variety ’Diana’
37-41Views:120An experiment on three dry bean varieties (Start, Hópehely, Diana), using different sowing-times, fertilizers and plant densities was performed on sandy soil in the University of Debrecen, Centre for Agricultural Sciences, Research Institute of Nyíregyháza in 2015. The aim of the experiment was to study which treatment gives the highest yield, and whether the different treatments result in significant differences in the yields. In this paper the dry bean yield at ‘Diana’ variety is analyzed.
The treatments were done with three fertilizer doses and three plant densities at different sowing-times (April 24; May 8; May 18). As a result of the high temperature and the drought during the growth season, the yields we harvested were in low, which shows the ecological sensitivity of the plant we examined.
We concluded that the poorest yield was harvested at the third sowing-time. There was no significant difference in the yields at the first and second sowing- time. Examining all the three fertilizer treatments we applied at the experiment, we achieved the highest yields in the control plots. It might be due to the weak efficiency of nitrogen fertilizer under the extremely dry conditions. The highest yield was harvested at the control treatments during the second sowing-time. Regarding the effect of the plant densities, the highest crop yield was achieved at the treatment using 400 000 germs ha-1, followed by 300 000 germs ha-1 and 200 000 germs ha-1.
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Effect and interaction of crop management factors and crop year on the yield of maize (Zea mays L.)
31-41Views:186The aim of this study was to determine the combination of treatment levels of crop management factors which can optimize and sustain maize yield under varying climatic conditions. The effect of winter wheat forecrop, three tillage systems (Mouldboard-MT, Strip-ST, Ripper-RT), two planting densities (60,000 & 80,000 plants ha-1), three fertilizer levels (N0-control, N80, N160 kg ha-1) with four replications in irrigated and non-irrigated treatments were evaluated over a five year period, 2015–2019. The obtained results revealed that growing season rainfall positively correlated with yield, whereas, temperature negatively correlated with yield. Impact of adverse weather on yield was less severe in biculture, irrigated plots, at lower planting density (60,000), lower fertilizer rate (N80) and in RT and ST, compared to MT. In years with favorable rainfall, yields of MT and RT were significantly (P<0.05) higher than ST. However, in a less favorable year, such as 2015, with 299 mm growing season rainfall and the lowest July rainfall (59% below mean) there was no significant difference (P>0.05) in yield among the three tillage treatments. Higher planting density (80,000), and fertilization rate (N160) in tandem with MT are treatments combination conducive for high yield under favorable climatic conditions, whereas, in years with low rainfall and high temperatures, RT and ST offer alternative to MT for optimum yield with 60,000 plants ha-1 and N80 treatment level. Crop year effect accounted for 20.7% of yield variance, fertilization 35.8%, forecrop 12.8%, plant density 3.4%, tillage 1.2% and irrigation <1%. It is conclusive that with proper selection of the appropriate levels of agrotechnological inputs the adverse effect of weather on yield can be mitigated.
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Heat treatment and microbial digestion of poulty feather for biogas production
215-219Views:94The aim of this research was the elaboration of the technological parameters of heat pre-treatment and microbial digestion of poultry feathers for biogas production. Feathers were treated at 70, 100, 140 °C, and subsequently digested by Bacillus licheniformis, or keratin disintegrator bacteria. Investigations focused on the optimalization of parameters influencing poultry feather biodegradation. The optimal range of pH, temperature,
feather size and bacillus:feather ratio were determined in the experiments, as well as the analysis of relationship between the examined parameters. In order to be able to track the dynamics of the biodegradation, we determined the extinction level of the liquid phase of the biodegraded material in the different experimental treatments. The results showed that the rate of hydrolysis was significantly higher in the treatments with bacteria than in the treatments without it. Regarding the pretreatments at 70, 100 and 140 °C, the digestion of feather was the most intensive at 70 °C. The most extensive digestion was observed in case of 1:3 feather:water ratio. The highest intensity of feather digestion was
detected in the treatment with 1% microbe ratio. -
Daily behaviour of Hungarian Grey Cattle under range grazing conditions
45-48Views:156Cattle behaviour on rangeland depends on external factors, such as grass allowance and quality, temperature, net solar radiation, distance from water-source, wind speed and direction, air pressure changes and the applied breeding technology. Our research is based on previous empirical observations and modern methods to analyze the behaviour of the Hungarian Grey Cattle. Today it is crucial to use cost-effective solutions in modern beef cattle farming therefore we introduce a cost-efficient method to study and follow cattle herds. We are studying relation between traveled daily distance and air pressure. The various weather fronts influence behavioural characteristics and traveled daily distance. According to our hypothesis, the pressure-change and the wind direction has significant effect on cattle activity on pasture. As the different air masses alter the barometric conditions and unbalance the neuroendocrine system, indirectly cause relaxed or agitated behaviour
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Responses of pea (Pisum sativum L.) to the rising atmospheric concentration of carbon-dioxide
185-188Views:163The atmospheric concentration of carbon dioxide increases from decade to decade in increasing pace. In 1957, atmospheric carbon dioxide levels were around 315 ppm, while in 2012 it amounted to 394.49 ppm concentration. In parallel, the global temperature is rising,which is projected to average 1.5–4.5 °C. The carbon dioxide concentration is a key factor – in interaction with the light – affects the plant's photosynthesis. Among the various factors significant interactions prevail: environmental factors affect - the growth and the development of plants, leaf area size and composition, the function of the photosynthetic apparatus, the duration of growing season.
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Timing it right: The Measurement and Prediction of Flowering
17-22Views:60Although the model described here was developed from research in controlled environments, there is now considerable evidence that in can be applied to a very wide range of natural environments in several species. Multi-locational trials augmented by successional sowing and, if considered necessary, supplementary illumination in the field to increase daylength, can be used to estimate the values of the model coefficients: (1) to characterize germplasm collections and so predict flowering behaviour elsewhere; (2) for interpreting and understanding crop adaptation; and (3) for genetic analysis of photoperiod sensitivity. We do not yet know whether the model has any contribution to make to the understanding of the biochemical mechanisms of photoperiod and temperature responses, but at the very least, it should provide the basis for indicating the most appropriate environmental conditions, genotypes and physiological stage of the plants most suitable for such investigations.
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Aflatoxin production on agricultural products
67-71Views:138Aflatoxins due to their toxicity pose significant economic and human health threat; therefore, it is important to avoid this type of contamination in agricultural products. Until now significant aflatoxin contamination occurred mainly in foods of tropical and subtropical origin because the optimal growth of the producer Aspergillus species is between 32–38 ºC. Nowadays the aflatoxin contamination is becoming higher threat in Hungary, due to the imported products, the rising average temperature and the climatic changes. There is a significant knowledge on the genetic and environmental effectors of the aflatoxin production; however, it is remained a great problem to control mold contamination and toxin production in farming and stock-raising. Here we attempted to summarize the knowledge on aflatoxin production and attempts of the elimination.
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The Effect of Atmosperical Aridity on the Changes of Quantitative Parameters of Horticultural and Arable Crops
40-45Views:103The occurence of atmospherical drought causes serious water-supply problems in the most cases of our domestic agricultural plant species. This paper was studied, how can we quantificate the atmospherical drought, with the help of a low input (relative humidityof the air, temperature) index. If this index (LSZI) characterized the atmospherical drought well, it will suitable to estimate the yield amount of agricultural plants.
The index elaborated by the authors was tested on county average crop yield of 14 agricultural plant species. Moreover we compared the atmospherical drought index (LSZI) to other aridity parameters, how suitable for estimate the yield amount.
Result of experiments show that, the atmospherical drought index (LSZI) can be used well by several agricultural plant species in especially coern and sugar-beet to estimate yield amount. Excellent results were found by comparison to other aridity indexes, this means it is worth using in the aridity researches in the future. -
Correlation between the weather in 2017 and the productivity of maize
89-93Views:152In our research we examined the effect of the hybrid, the nutrient supply, the number of plants and the abiotic factors (temperature, amount of precipitation) on the yield, crop quality and yield stability of maize. We devoted special attention to the natural nutrient utilization ability and fertilizer reaction of maize. The experiment took place in Hajdúszoboszló on chernozem soil, on a nearly 8 ha field. The size of one plot was 206 m2; therefore, this experiment was half-industrial. We tested six hybrids with different genetic characteristics and growing seasons. I analysed the correlation between the nutrient supply and the yield of maize hybrids with control treatment (treatment without fertilization) and with N 80, P2O5 60, K2O 70 kg ha-1 and N 160, P2O5 120, K2O 140 kg ha-1 fertilizer treatments. The yield increasing effect of the fertilizer also depended on the number of plants per hectare to a great extent. The number of plants of the six tested hybrids was 60, 70, and 80 thousand plants ha-1.In Hajdúszoboszló in 2017, up to October, 445.8 mm of rain fell, which is in line with the average values of 30 years, and is only 45.7 mm less than those. In 2017, the effect of increasing the plant number was slighter. Averaged over the observed fertilizer treatments and hybrids, the yield was 9.10 t ha-1 with 60 thousand plants ha-1, 9.11 t ha-1 with 70 thousand plants ha-1 and 9.12 t ha-1 with 80 thousand plants ha-1. Without fertilization, in most cases, increasing the plant number from 60 thousand plants ha-1 to 70-80 thousand plants ha-1 does not increased the yield but decreased it. With N80+PK treatment the yield changed between 8.90 and 11.27 t ha-1. The effect of increasing the plant number was just slightly observable and did not show a clear tendency. The effect of changing the plant number, even with the highest dosage of fertilizers, could not be detected adequately. In contrast with the plant number, the effect of the different fertilizer treatments was expressly traceable. Compared to the control treatment (treatment without fertilization), with N80+PK fertilizer dosage with 60 thousand plants ha-1 the yield increased by 3.36–4.99 t ha-1. The smallest demonstrable proof, i.e. the LSD5% was 0.22 t ha-1, which means that fertilization, in each case, significantly increased the yield. When analysing the effect of fertilization in the average of the hybrids and the different plant numbers, a yield of 5.61 t ha-1 could be detected, which value was 10.12 t ha-1 with N80+PK treatment and 11.61 t ha-1 with N160+PK treatment. Thus, it can be calculated that compared to the treatment without fertilization, the N80+PK treatment increased the yield by 4.51 t ha-1, while compared to the N80+PK treatment, the N160+PK treatment increased the yield by 1.49 t ha-1. In addition to agrotechnical factors, in maize production, the impact of the crop year is specifically of high importance.The average yield of hybrids (in the average of the different fertilizer treatments) was 6.81 t ha-1 in 2015, 11.86 t ha-1 in 2016 and 9.11 t ha-1 in 2017. When comparing the yield results against the precipitation data, it is clearly visible that the amount of rain fell in the January– October period is directly proportional to the average yield of maize. The effect of the crop year can be defined in a 5.05 t ha-1 difference in the yield.