Search
Search Results
-
DHAC-induced transgene overexpression in 35S-gshI GMO poplar (Populus × canescens)
78-83Views:33Relative gene expression levels of transgene gshI (γ-glutamylcysteine synthetase cloned from E. coli) were analyzed by qRT-PCR in two transgenic poplar (Populus × canescens) clones (11ggs and 6lgl) and wild type (WT). An extremely high expression level of transgene gshI was observed in the 6lgl clone (13.5-fold) compared to 11ggs (1.0) samples, which level was doubled (1.8-fold) in the DHAC (5.6-dihydro-5'-azacytidine hydrochloride) treated (at 10-4 M for 7 days) 6lgl samples but not in the 11ggs clone (0.4-fold). Contrary to this result, relative copy number of transgene gshI in the 6lgl clone was found to be less 60% less (1.0) then in the 11ggs samples (1.6). Relative expression levels of proper poplar gene gsh1-poplar showed significantly higher responsiveness to DHAC treatment than transgene gshI with the highest expression level in the untransformed (WT) poplar
clone (19.7-fold) compared to transformed 6lgl (8.7-fold) and 11ggs (2.5-fold) clones. For internal controls constitutively expressed housekeeping genes a-tubulin were applied. For data analysis, the 2−ΔΔCt method was used. DHAC applied in long-term cultures (27 days) at low concentrations (10-8 – 10-6 M) showed morphogenetic activity by initiating de novo root development of leaf discs. -
Influences of water deficiency on the productivity of young plants at different sites
371-378Views:120Water deficiency has become one of the most limiting factors of crop production in Hungary as the tendency in annual amounts of precipitation shows a decreasing tendency; therefore, it has become similar to those of Southern Europe. The most significant decrease in precipitation occurs typically during spring, approximately 20% of the data expressed in the averages of the last century. Studying the relationship between water deficiency as a stress factor and nutrient supply is important in order to improve the production efficiency of crops. Nowadays, this problem receives outstanding attention presented in numerous papers both in Hungary and globally, however, there are several questions yet to be answered. Our pot experiments were carried out under controlled greenhouse conditions in order to establish new data on these relationships. Experimental soils were typical for Western Transdanubia, taken from long-term field experiments representing four different site characteristics of the region. It was concluded from the results that drought periods during the early growth stages (i.e. 4–5 weeks after emergence) of plants may result in significant decreases in both dry matter production, nutrient concentrations, nutrient uptake and shoot:root ratios. Better nutrient supply, especially potassium, plays a significant role in reducing the negative effects of water deficiency.
-
Correlation between cultivation methods and quality in some vegetable species
313-317Views:111Quality parameters of 5 table root varieties were tested on 3 sowing dates with different cultivation methods: open field on 15 April and 9 July 2010 and under plastic tents on 19 August. The highest red pigment content (betanin) was measured in the varieties Akela and Mona Lisa (~ 80 mg 100 g-1) of the second (July) crop. This crop is in general use in Hungary. In comparison, in the late sown varieties (August, under plastics) a further pigment increase (10–20 mg 100 g-1) was observed in the same varieties as related to the earlier sowing dates. Yellow pigments (vulgaxanthins) showed similar trends. Roots of the late sowing date (with harvest in December) contained the highest vulgaxanthin values (103.3–124.18 mg kg-1).
Varieties reacted differently to temperature changes during the production period and thus to sugar accumulation. In the second crop (July) higher water soluble solids content was measured on the average of varieties (10.12%) in comparison to the April sowing (7.76%). Beetroots of the spring sowing are recommended for fresh market while the second (July) crop with autumn harvest can satisfy industry requirements. Late sowing under unheated plastic tents supply us with fresh beetroot in late autumn and early winter and prolong the usability of plastic tents.
Six lettuce species/subspecies were tested in the open field and under plastic tents in 3 repetitions for nitrate nitrogen, vitamin-C, polyphenol (gallus acid equivalent – mg GAE 100 g-1) and mineral element (Ca, K, Mg, Na) contents. Our measurements showed lower nitrate nitrogen values under plastic than in the open field (89.10± 8.13 and 127.06±14.29 mg kg-1) on the average of genotypes. Lettuce grown in the field had higher vitamin-C content (1.4 mg%) which is nearly 50% more than in plants under plastic. The highest polyphenol content was found in samples from the field with a conspicuous value of 804.17±56.47 mg GAE 100 g-1 in Piros cikória. Samples grown under plastic were richer in mineral elements (Ca, K, Mg, Na) which can be explained by the higher nutrient content of the soil. In this environment superior Mg content was observed in Edivia (4616.33±
311.21 mg kg-1).Besides the well- known headed lettuce, Piros cikória (Red chicory),the red leaved Lollo Rossa and Tölgylevel (Oak leaf lettuce) should be
mentioned which well deserve further testing in order to supply us with nourishing, healthy food. -
Exogenous salicylic acid treatments enhance tolerance to salinity of wheat (Triticum aestivum) plantlets
34-38Views:118Salt stress, an abiotic stress, determines modifications of some biochemical indicators, like, antioxidant enzymes, proline (amino acid
accumulate in higher plants under salinity stress) content, and some physiological processes including: plant growth and development. In
this paper we studied the influence of exogenous treatment of wheat seeds, with 0.1 mM salicylic acid (SA) solution, in the plant response to
salt stress. The treatment was applied by presoaking the seeds in the treatment solution for 12 hours before germination. The results showed
that exogenous 0.1 mM SA solution, administrated to the wheat cariopses significantly ameliorated the negative effect of salt stress in first
week of germination in laboratory conditions. -
The Effect of Mg Foliage Fertilization on Beetroot (Beta vulgaris ssp. esculenta var. rubra) Morphology, Quality Traits and their Relations
67-72Views:91Mg-treatment increased solid soluble content in roots probably due to the larger assimilation area.
The applied foliage fertilization affected inner colour intensity and uniformity in roots positively.
The 2% Mg-sulphate solution affected pigment quantity favourably depending on varieties.
Close correlation (r=0,71) was found between the yellow (BX) and red (BC) pigments which indicates a similar biosynthetic pathway of the two compounds.
The Mg-sulphate foliage fertilization improves quality considerably but the individual reaction of varieties needs further studies. -
Fusarium culmorum isolated from rhizosphere of wooly cupgrass (Eriochloa villosa) in Debrecen (East Hungary)
93-96Views:143Wooly cupgrass (Eriochloa villosa) is an East-Asian originated weed species and it has been spreaded worldwide by now. The first occurrence of this species in Hungary was observed and published in 2008 nearby Gesztely village (Borsod-Abaúj-Zemplén county, North-East Hungary) than in the summer of 2011 a significant population was discovered next to Debrecen city (Hajdú-Bihar county, East Hungary).
In 2013 this weed was also reported from Szentborbás village, Somogy county (South-West Hungary). These observations of spreading and its biological features (production of stolons and large number of seeds, moreover herbicide tolerance) indicate that wooly cupgrass (E. villosa) has a great potential of invasiveness, so it may become a hazardous weed not only in Hungary but in all over the world.
The objective of this study was to identify the fungus which was isolated from wooly cupgrass (E. villosa) root residue samples which were collected after maize harvesting on arable land in late autumn, near Debrecen. The identification of the fungus based on morphological characters of colonies and the features of conidia developed on potato dextrose agar (PDA) plates. After the examination of axenic culture we revealed that the fungus from rhizosphere of wooly cupgrass was Fusarium culmorum. Pathogenicity and/or endophytic relationship between the fungus and wooly cupgrass is still uncertain so pathogenicity tests and reisolations from plants are in progress.
-
Violation prooxidative-antioxidant stability at maize shoots at different level of accumulation of cadmium and nickel
89-94Views:66Joint influence of cadmium and nickel was investigated on the feature of their accumulation by the vegetative organs of 10-days' old maize shoots. It was established that most intensively noted metals are taken in by the roots of shoots in the first 7 hours stressing influencing, while in leaves they appear only after a 7-hour long exposition. It was stated that the absorption process of the noted metals by a root system is carried by two-phase character. The indexes of inner-tissue contamination are calculated. Activating by the cadmium and nickel ions of lipid peroxidation as marker of the stressing influencing, and also was shown the proper increase of intensity of functioning of ascorbate peroxidase as the antioxidant enzyme protection of cell.
-
The impact of various grape stock cultivars on the As, Cu, Co and Zn content of the grape berry (must, seed)
39-44Views:133Scientific research from the last decades showed that the inappropriate industrial and agricultural production caused an abnormal increase of the potentially toxic elements in the soil. Unfortunately the acidification of the soil is an increasing problem in Hungary. According to Várallyay et al. (2008) 13% of the Hungarian soils are highly acid. Accumulation of toxic elements differs in the genetically diverse plant species. The root of the plant constitutes a filter so that the rootstock is also kind of a filter system, which may prevent that the scion part (such as berry) accumulate high levels of various potentially toxic elements from the soil. The aim of research was to determine how different grape rootstocks influence the As, Co, Cu and Zn content of the musts and seeds. Thus, specifying which of the grape rootstocks takes up the lowest level of these 4 elements (As, Co, Cu and Zn), and accumulates in berries, so could reduce the potentially toxic element load of the grape berries. The grape rootstock collection of the University of Debrecen was set up in 2003 in 3x1 m spacing on immune sandy soil. Grafting of ‘Cserszegi fűszeres’ was started in 2010. We could evaluate yields harvested from 12 rootstock varieties of the experiment in October 2011. We obtained valuable differences in the arsenic, copper, cobalt and zinc concentrations of musts and seeds of ‘Cserszegi fűszeres’ grafted into different rootstocks. The results obtained from the 2011 harvest support the statement that the choice of rootstock might be an important factor to increase food safety. The differences in concentration of the four elements observed in case of the rootstock may have been caused on one hand by the rootstock effect, and on the other hand, the vintage effect has a very significant impact on the vines element uptake. Several years of experimental results will be needed to answer these questions.
-
The role of the hybrid-specific technological recommendations of maize in precision crop production procedures
297-302Views:87The necessity of application of hybrid specific crop production technology has been confirmed not only by trial results but also by the experiences gained from the agricultural practice. For this reason it is essential to test and collect data in field trials about the specific agronomic traits of the corn hybrids belonging to different maturity groups and genotypes. Corn hybrids are tested for their responses to sowing time, plant density and fertilizer supply; sensitivity to herbicides; and lately, the resistance to the damages caused by the larvae of corn root worm. Last but not least, mention should be made of the differences in the responses of the corn hybrids to the damages caused by drought stress. Based on the trial results, suggestions for the hybrid specific corn production are compiled and made public for the experts and farmers engaged in corn growing. Corn hybrids may deliver maximum yields on the impact of specific crop production technology only in case if it relies on carefully done general production technology including soil cultivation, seed bed preparation and weed control. Similarly, precision crop production technology may advance the yield increase in economic way if it is constantly drawing on the source of research results.
-
The influence of crop rotation and nutrition regime in winter wheat on the phytomass remains and the deposits of weed seeds remained in the ground
10-13Views:126An important problem refers to soil weeds seed bank with special reference to brown luvic soils and relationship between vegetal remains
(roots + stub) and nutrition regime – crop rotation plant. A great number of seeds can be found in first 20 centimeters of soil (plowing
layer). This fact causes a high weeds density, which has a negative effect on the cultivated plants leading to partial or total crop loss. -
The Effect of Smut Gall Tumour Infection on Iron and Zinc Uptake and Distribution in Maize Seedlings
27-32Views:87The amounts of Fe, and Zn were measured in maize seedlings infected by smut gall tumour (Ustilago maydis Dc. Cda.) and in healthy seedlings five days after infection. The amount of elements was also measured under different stress intensities. Due to the infection, as a biotic stress, the amount and distribution of examined elements have been changed. On the bases of the differences in the Fe distribution between the symptoms less and tumorial leaf parts, we have come to the conclusion that the infection also effects the mobilisation of Fe and Zn inside the plants. The Fe uptake was much higher in the infected plants and the tumour development also had an effect on the uptake and distribution of the examined elements. The experiments of infecting maize seedlings by monosporidial strain of crown gall tumour showed no tumour development. We found that the monosporidial strain also acts as a biotic stress and has an effect of iron and zinc distribution. We observed a slight difference in the iron and zinc contents in the roots of corn seedlings infected by different monosporidial sporidium concentrations, while the iron and zinc contents in the shoots were increased by the intensity of the infection. The roots do not form tumours. There is no difference between the roots of the infected and healthy corn seedlings. Since the Fe and Zn contents of the shoots of infected plants depend on the intensity of the infection, we have come to the conclusion that there must exist a „special” communication system regulating the transportation of the examined elements.
In the experiments with infected maize seedlings, it became necessary to get the iron chlorosis before the disease reaches the lethal phase. Although most of the iron reserves are located in the embryo, to accelerate the chlorosis, the endosperm was removed, and it was observed, that the iron chlorosis appears later in maize seedlings when the endosperm is removed. The relative chlorophyll content of the first and second leaves was measured in iron efficient and iron deficient maize seedlings at different times.
The higher IAA content of tumorial plant tissues is already known. The treatment with IAA decreases the iron concentration in the shoots and in the roots of +Fe precultured plants and increases at -Fe precultured ones. The TIBA retards the shoot-to-root transport of IAA. When the seedlings were treated simultaneously with IAA and TIBA, higher iron concentrations were observed in the shoots and in the roots of corn seedlings.
We found extremely high iron concentrations in the roots of infected seedlings and, in line with this, serious damage to the roots was observed that this can be caused by the high iron content generated free radicals. The results demonstrate that IAA has a role in the shoot to root communication. -
Influences of different organic fertilizers on nutrients of humic sandy soil and on the growth of Spinach (Spinacia oleracea L.)
23-28Views:292A greenhouse pot experiment was conducted to compare the effects of manure with different origin (horse, cattle), various bedding materials (straw, sawdust) and diverse doses (30 t ha-1, 60 t ha-1) and the impact of food waste compost on the plant growth and the available plant nutrient content of soil. The study was conducted on humic sandy soil and consisted of 9 treatments in a randomized complete block design with four replications. Spinach (Spinacia oleracea L.) was grown as the test crop. The treatments were: 1. unfertilized control; 2. horse manure with straw (30 t ha-1); 3. horse manure with sawdust (30 t ha-1); 4. cattle manure (30 t ha-1); 5 food waste compost (30 t ha-1); 6. horse manure with straw (60 t ha-1); 7. horse manure with sawdust (60 t ha-1); 8. cattle manure (60 t ha-1); 9. food waste compost (60 t ha-1). Plant growth was monitored for 4 weeks. Shoot and root weights per pot were measured, total biomass weight per pot were counted.
On the basis of the results it can be concluded, that among treatments the application of horse manure with straw enhanced spinach growth most significantly compared to other treatments and to the non-treated control, resulted the highest weights of leaves and roots of spinach. At the same time even small dose (30 t ha-1) of this fertilizer caused increased plant available nitrogen and phosphorus of soil and the higher dosage further increased these values. The horse manure with sawdust applied in lower dose did not alter the leaves and roots weights, but higher portion (60 t ha-1) caused significantly decreased plant biomass. The results proved that the bedding material may significantly alter the composition of manure and may change the plant nutrition effect of organic fertilizer. Cattle manure and food waste compost in both applied doses enhanced plant growth. Both fertilizers increased the plant available nitrogen forms and phosphorus content of soil, but cattle manure caused higher increase.
-
The potential of biological control on invasive weed species
73-75Views:41Sorghum halepense is one of the invasive species in Europe. This study was made to identify the morphology of fungi on invasive weed species samples on the roots of Sorghum halepense. The samples were collected in the region of Debrecen. The experiment was conducted under laboratory conditions to determine the microscopic form of fungi. The samples were put on PDA and for identification of fungi is based on the morphological characteristics of the features and colonies of conidia that were developed in Petri dishes.
The examination of the culture revealed that the fungus from the root of Sorghum halepense was Aspergillus niger. Pathogenicity and the relationship between the fungus and Sorghum halepense are still uncertain so in the future pathogenicity tests and re-isolations from plants are very important steps.