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Soil analysis as the foundation of precision nutritive supply in the Hajdúszoboszló region
141-148Views:71Larger cultivated plots are heterogeneous from a pedological aspect. Heterogeneity causes problems during fertilization and harvest. The heterogeneity of cultivated areas can be compensated by fertilization which is based on soil analysis. We carried out research into the changes of the soil on three soil types, from 1966 to 2006, on the cultivated areas of Hajdúszoboszló.
There were no significant changes in pH on chernozem meadow soil and meadow chernozem soil, but the pH increased in 0-30 cm layer on type meadow solonetz soil. The saline content decreased in every examined soil type. Decrease was the largest on meadow solonetz soil. Decline of humus content was the largest (0.95%) on chernozem meadow soil, and the smallest (0.39%) on meadow chernozem soil. The nitrogen content decreased with 528 ppm in the 0-30 cm layer on chernozem meadow soil, and decreased by 186 ppm on meadow solonetz soil. Phosphorus and
potassium content increased in every examined soil types. Rise of phosphorus content was 188.9 ppm in the 0-30 cm layer on meadow chernozem soil. The potassium content rose by 153.7 ppm on this soil type. Phosphorus content increased with 70.8 ppm, and potassium content increased by 57.6 ppm from 1966 to 2006. -
Labour market characteristics of Ukrainian employees with special regard to the North Great Plain region
67-72Views:128National Employment Public Foundation founded our research to examine the employment characteristics and job market importance of Ukrainian citizens in Hungary in 2008. As the means of our investigation we analysed available statistical data and conducted a survey. Ukrainian citizens working in Hungary with valid work permit were questioned. 226 people were in the sample.
Hungarian job market processes were not significantly affected by the officially employed Ukrainian citizens because their number was rather small in relation to the number of registered unemployed people and in relation to the available job positions. It can be stated that Ukrainian citizens mainly work in positions where high specialised knowledge is not a requirement.
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Comparison of Pálfai’s drought index and the Normalised Precipitation Index in the North Great Plain region
59-64Views:152Agriculture has always been an important role in economy, food supplies, sustainability of society and creation of job opportunities in Hungary. Our country has resource-related strength of agriculture, because we have more than 4.5 million ha for agricultural production. Agricultural production can be influenced by several factors, including climate, hydrology, soil conditions and antropogenic impacts. Climate determines the quality and quantity of the crop yields. The climate conditions in Hungary are variable and it shows spatial and temporal extremes. As a result of this, drought have become more frequent in our country (2003, 2007, 2009, 2012), which is reflected in the decline in yields as well. In the present study, Pálfai's Drought Index (PAI) and the Standardized Precipitation Index (SPI) were compared 2003–2012 in Debrecen. The temperature and precipitation data were calculated from data provided by a local meteorological station to work out PAI, while the SPI-3 index values were downloaded from the database of the European Drought Observatory. This allowed to drought assessment in a local and regional scale. Our study was supplemented with SPI-3, soil moisture anomalies, PAI and yields of wheat (Triticum aestivum L.) and maize (Zea mays L.) to evaluating the impact of drought on agriculture.
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The role of Local wine administration bodies in creating social and cultural capital and in the preservation of local identity
135-143Views:76Due to the peculiarities of viniculture, some kind of a community organizing force has always existed in every European wine region. The Hungarian system of local wine administration
bodies is however unique. The foundations of the system have hardly changed throughout the centuries and the communities’ function as a socio-organizing power has been historically proven. In this essay I am attempting to demonstrate this power and ability using social, ethnical, sciences, anthropological, landscapeeconomical and occasionally mathematical methods. As former leader of a local wine administration body in Egerbakta, the member of the same body in Eger and also a vine-grower in Létavértes I also have first hand information about the practical issues of the system. The paper could not be more timely after the 2006’s modification of legislations governing wine administration. One element of the modifications is the creation of large and centralized administration bodies over the ruins of small ones that
were presumed to be indispensable for a long time. -
Comparison of the sample preparation methods worked out for the examination of the element content of wine
77-82Views:113The examination of the potentially toxic elements content of the wines is not easy task, because the most elements are in little concentration (mg kg-1 or μg kg-1) in the wine and the wines contain great amount of organic matrix. The efficient sample preparation is essential for the accurate determination of element content. The eim of our research was to determine which sample preparation method will be the most efficient in examination of wines with ICP technology. The examined wine sample was a 2008 Chardonnay from the Eger wine region. We did the sample preparation and analysis examination in University of Debrecen, Centre for Agricultural and Applied Economic Sciences, Institute of Food Science, Quality Assurance and Microbiology.
We did the analysis examinations with ICP- MS (inductively coupled plasma mass spectroscopy). We always did the sample preparations and the examinations in three times rehearsal. The applied sample preparation methods: dilution with distilled water, open digestion and microwave digestion.
We were able to measure B, Al, Mn, Fe and Zn with only dilution and open sample preparation. In the smaller quantity present Sr and Ba were measurable in the wine in the case of all three methods well. We were able to measure the Co with dilution and open digestion method, while Cr, Ni, and Te with only dilution method. In the case of arsenic we were not able to measure reliable result with dilution and open digestion method because of organic matrix and other components
(alcohols, monosaccharides, polysaccharides, polyalcohols and inorganic salts). On the whole we are able to say that in the case of certain elements (B, Mn, Fe, Zn, Sr, Ba) the open digestion and dilution sample preparation is applicable well, however, in the case of certain elements (As, Al, V, Cr, Se, Mo, Cd, Hg, Pb) we have to develop the methods. It may be development of one of the way, if we develop sample preparation methods to examined element specifically and not
to wine generally. -
Fertilisation Effect on Quantity of 0.01 M CaCl2 Soluble N-Forms in a Long-Term-Small-Plot Experiment
166-170Views:45long-term experiments have an opportunity to investigate the effects of fertilization and plant nutrition. The paper reports the results achieved in the 39th years of a long-term-small-plot fertilisation and liming experiment set up on acidic sandy brown forest soil in the Nyírség region. From the 32 treatment, four replications, altogether 128 plot experiments with 10 treatments are summarized. We took samples after harvest of triticale, in August.
We used a reliable method (segmented continuous flow analysis) to determine different (easily mobilized - 0.01 M CaCl2 soluble) N-forms of soil. The 0.01 M CaCl2 soluble inorganic and total N content and the UV digestable organic-N form of soil were determined by this method.
The results are summarized below:
– The mineralized-N (Nmin.) content of soil increased with dose of nitrogen treatment. Liming treatments increase the amount of Nmin.
– The maximum content of easily mobilize organic-N-fraction was found in the upper (0-20 cm) layer. This fact due to the large amount of crop and roots.
– Changing of content of 0.01 M CaCl2 soluble total-N-forms due to N doses.
– The ratios of these N forms are variable. It is very important that the content of organic N fraction is not negligible and this fraction plays a main role in the plant nutrition. -
Application of environmental accounting tools for agricultural companies
60-68Views:74Today there is an ever stronger requirement for companies to consider the environmental aspects of their operation. The incorporation of these aspects into the economic practise of agricultural firms in our country is still in its infancy and, moreover, it usually follows from outside coercion (legal regulation) rather than voluntary behaviour.
Consequently, in the Hungarian agrar-economics – contrary to the example of Western Europe – the application of environmental accounting in agriculture is a barely researched subject; this has been so even though the environmental policy of companies can be made more conscious by adding an environmental dimension to the organizational information systems.
The aim of our investigation is two-fold. The first goal is to form a method that quantifies the environmental load of companies, is capable of comparing environmental performance of companies with different sizes, and takes into account the specific attributes of agriculture as well. The second is to apply this method in practice, to prove that estimating the environmental load of companies is possible by processing already available data (fiscal, accounting and statistical) using new concepts.
In this publication we present the joint application of three methods – each belonging to the toolset of environmental accounting – for two companies dealing with both crop and animal farming in the Pannonian region. The three methods are partly built upon each other and partly complementary. The company-level environmental balance is capable to show the relations between a given firm and its surrounding, but realistic comparison between environmental performance of different companies or periods is only possible by using specific index quantities. -
Correlation between body condition change and reproduction parameters of Holstein-Frisian cows after calving
49-52Views:93The authors examined the body condition with relation to reproduction of Holstein-Friesian cows on three dairy farms in the South Plain region. During the examination they analyzed the data of 516 births of 494 Holstein-Friesian cows. They were trying to find correlation between the first service, the service period and the service rates. The examinations with regard to the correlations were different from the ones published in the professional literature. The body condition at the time of calving affected the interval from calving to the first service but not the conception
rates or the service period. The examinations did not prove any correlations between body condition changes and reproduction. -
Characterization of Hungarian touristical turnover 2006-2009 – focus on health tourism
47-50Views:123Tourism is one of the biggest and mostly increasing branch all over the world. Since it is getting more and more significant in Hungary as well in this article I analyze the turnover of Hungarian tourism on the basis of guest nights spent on commercial lodgings. I examine its changes in time and its territorial spreading. Finally I explore the health touristic volume, because it is one of the most important touristic product of Hungary and the North Plain Region and the infrastructure based upon preserves unique opportunities both for health and wellness tourism.
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Combined traffic control of irrigation on heterogeneous field
187-190Views:171In arid areas, such as Hungary, most climate models forecast a rise in water scarcity. Irrigated land accounts for 2% of agricultural land in Hungary, with most irrigation technology being relatively outdated. The aim of this research was to lay the foundation for a combined traffic management system for a water-saving precision irrigation system on an 85-ha field in the Tisza River basin's reference region. High-precision soil maps were created to support the water-efficient variable-rate irrigation system by selecting and selecting areas for different agrotechnical implementations and precision farming zones.
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The Dg RES generation, storage, utilization and integration program ’1 village – 1 MW’ of the Bükk-Miskolc Region, Phase 1–3.
233-235Views:84The usage of renewable energy sources (RES) and the increase of energy efficiency could be the solution for the difficulties of the rural impoverished inhabitants. A rural development company with the support of the communities designs the development resources from the EU and the Hungarian State for RES generation and organizes the ’1 village – 1 MW’ RES generation, storage, distribution and usage integration.
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The Bereg flooding in 2001 – Impacts on the built environment
99-106Views:101Today, an increasing number of natural disaster events are witnessed. Our respond to these disasters is increasingly important and more timely problem. Before PhD studies my research aimed at revealing the impacts reconstruction, restoration and damage prevention had on the environmental, social and economic segments of disaster-hit areas. This present paper intends to provide preliminary results of the work already carried out in the Bereg Region, where I specialized on the effects of Tisza flood.This paper focuses on the results and conclusions drawn from the analysis of environmental consequences resultant from the flood based on a study area.
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Influences of water deficiency on the productivity of young plants at different sites
371-378Views:120Water deficiency has become one of the most limiting factors of crop production in Hungary as the tendency in annual amounts of precipitation shows a decreasing tendency; therefore, it has become similar to those of Southern Europe. The most significant decrease in precipitation occurs typically during spring, approximately 20% of the data expressed in the averages of the last century. Studying the relationship between water deficiency as a stress factor and nutrient supply is important in order to improve the production efficiency of crops. Nowadays, this problem receives outstanding attention presented in numerous papers both in Hungary and globally, however, there are several questions yet to be answered. Our pot experiments were carried out under controlled greenhouse conditions in order to establish new data on these relationships. Experimental soils were typical for Western Transdanubia, taken from long-term field experiments representing four different site characteristics of the region. It was concluded from the results that drought periods during the early growth stages (i.e. 4–5 weeks after emergence) of plants may result in significant decreases in both dry matter production, nutrient concentrations, nutrient uptake and shoot:root ratios. Better nutrient supply, especially potassium, plays a significant role in reducing the negative effects of water deficiency.
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Application of GIS, precision agriculture and unplugging cultivation in plant breeding of Karcag
49-56Views:151In the last two decades, the prevailing ecological conditions and climate change have caused negative effects. Therefore, a paradigm shift is needed in the field growing of plants. The latest inventions, digital technologies, precision cultivation are not enough, the mentality of the farmers is more important. For this reason, not only big financial sacrifices, but adequate receptivity are needed on behalf of farmers. Adequate skills and continuous self-education are necessary. The yield of plant growing farms is determined by ecological conditions to a 40% extent, genetic background of the seed has a 30% share and the applied agricultural technology has a proportion of 30%. In different agroecological conditions, bred varieties of plants have bigger tolerance to unfavorable factors of the regions and significant yield stability. Farmers, who buy and sow seeds, can only contribute to the genetic potential of the seeds with cultivation technology. Plant breeding provides stable genetic background and good quality seeds. Breeding activity – choosing variety proposants, breeding them, selection work, classical breeding process for 8-10 years – must create new landraces, which can produce balance, high yield and have good quality parameters in extreme ecological conditions, yearly excursion and have higher tolerance to unfavorable factors of the region giving significant production stability for farmers. In Karcag GIS technology, precision cultivation elements and soil-friendly agrotechnical methods have been introduced which largely support the aims of breeding and can also provide optimal cultivation conditions in extreme years. Because of the specificity of breeding the main aim is not only to increase yield but to provide harmonic growing for bred materials, to decrease the number and the cost of cultivation and to be punctual. In this study, applied new methods and technologies are introduced.
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The zooecological remediation of technogen faulted soil in industrial region of Ukraine steppe zone
111-115Views:72In Ukraine’s Steppe zone the extraction of minerals is important. To eliminate the consequences of coal mining the agricultural recultivation of the disturbed soil is used. Thus toxic compounds for human beings and the majority of plants and soil biota representatives, which can be found mining rock, get into plants and invertebrates by trophy chains. When remediating soil, it is necessary to create tropic conditions in order to provide the life of soil biota. Earthworms (Lumbricidae) are the primary decomposers of the organic material. They are numerous in soil and facilitate the improvement of natural and artificially created soil. This paper studies the possible influence of different variants of substrates, which are used in re-cultivation, the leaf litter from leaves of different wood species, as well as different levels of humidity on the representatives of soil saprophages. Optimal variants of artificial mixed-soil providing the stable existence of animals have been shown, which are recommended for the implementation of rehabilitation measures.
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Role of the landscape in spatial and rural development
37-41Views:89The study is going to reveal the role of landscape potential in spatial and rural development based on the analyses of scientific literature. The target area of the research is three micro regions in Hajdú-Bihar county. In addition to the analyses of scientific literature the study pays attention to the assay of local development concepts and programmes. The conclusion of the study is that the analyses of landscape changes on micro regional level are obstructed by the lack of micro regional spatial development and substantive landscape plans.
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The potential of biological control on invasive weed species
73-75Views:41Sorghum halepense is one of the invasive species in Europe. This study was made to identify the morphology of fungi on invasive weed species samples on the roots of Sorghum halepense. The samples were collected in the region of Debrecen. The experiment was conducted under laboratory conditions to determine the microscopic form of fungi. The samples were put on PDA and for identification of fungi is based on the morphological characteristics of the features and colonies of conidia that were developed in Petri dishes.
The examination of the culture revealed that the fungus from the root of Sorghum halepense was Aspergillus niger. Pathogenicity and the relationship between the fungus and Sorghum halepense are still uncertain so in the future pathogenicity tests and re-isolations from plants are very important steps.
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Current Conditions and Opportunities of Biofarming in Hungary
150-156Views:85The aim of organic farming is not to maximize income, but to achieve optimal product quality. It is completed by the tightest possible material, and energy flow within the farm. Organic agriculture significantly reduces external inputs by avoiding the use of chemo-synthetic fertilizers, pesticides and pharmaceuticals. Instead it works with nature to increase both agricultural yields and disease resistance. Total independence of external resources can not be achieved in Hungary due to the small-scale of organic animal husbandry. Some materials in limited quantities can be purchased from external resources, though the group of these materials is strictly regulated. Organic farming harmonizes with the concept of European multifunctional agriculture, because besides farming, it includes social considerations, as it helps to maintain natural resources and the relationship between people and their environment, and provides a living for those living in the region.
As regards organic farming the fertility of the soil and the health of vegetation can be influenced in various ways. Farmers have to be highly skilled and able to manage a farm with great expertise. Generally it can be stated that as the use of non-organically produced products is limited, the opportunities to correct failures made by the farmer are minimal, contrary to conventional farming. Farmers must be intent on developing the tightest material- and energy flow. This means that organic farms ideally have both animal husbandry and crop production. This energy and skill demanding system of farming is compensated by state subsidies, growing market share and relatively high prices for organic products. -
Phylogenetic analysis of Phoma species
100-107Views:92The cosmopolitan Phoma genus contains mainly phytopathogenic, opportunistic parasites, and saprophyte fungal species. Up to now, the characterization of Phoma species and other taxa of Phoma has been determined on the basis of morphology on standardized media, and gene sequence analysis was only used as a confirmative or distinctive complement.
In this study, we tried to find molecular markers which can be used as phylogenetics markers in the molecular based classification in the Phoma genus.
We employed a part of the translation elongation factor 1 subunit alpha (EF-1α=tef1) containing both introns and exons and ITS region containing the internal transcribed spacer regions 1 and 2 and the 5.8S rDNA, as potential genetic markers to infer phylogenetic relationships among different Phoma taxa. Twelve different Phoma species sequences were analysed together with the closely related Ascochyta ones. The constructed phylogenetic trees, based on tef1 and ITS sequences, do not support the traditional Phoma sections based on morphological characterization. However, we managed to distinguish between the Phoma strains and Ascochyta species by comparing their tef1 sequences through parsimony analysis. We proved that a tef1 can be a useful phylogenetic marker to resolve phylogenetic relationships at species level in Phoma genus.
Both parsimony sequence analyses confirmed that the Phyllosticta sojicola species is identical to the Phoma exigua var. exigua species as Kövics et al. (1999) claimed. However, the evolutionary distance by ITS sequences within Phoma species is too small to get well based consequences for the phylogenetic relationships of Phoma genus.
Further investigations would be necessary to clarify whether the tef1 and ITS sequences as phylogenetic molecular markers are well suited for the classification of Phoma species. -
Site and hybrid-specific agrotechnical models in sweet corn production
105-108Views:92The effect of three agrotechnical factors (sowing time, fertilization, plant density) and two genotypes on the crop yield of sweet corn was examined on chernozem soil in the Hajdúság region in two different crop years. Compared to the 30-year average, the climate was dry and warm in 2009 and humid in 2010. The experiments were conducted at the Látókép Research Site of the University of Debrecen. In the experiments we applied two sowing times (end of April, end of May), six fertilization levels (control, N30+PK, N60+PK, N90+PK, N120+PK, N150+PK) and two crop density levels (45 thousand ha-1, 65 thousand ha-1). The hybrids we used were Jumbo and Enterprise. As regards the requirements of sweet corn production, the crop year of 2009 was dry and warm. The effect of moisture deficiency was more adverse on the crop yields with the second sowing time. On the contrary, the other examined year (2010) was significantly humid; the precipitation was 184 mm above the 30-year average and the temperature was average.
In the dry and hot crop year, the best yields were obtained with the hybrid Jumbo (25677 kg-1) at 65 thousand ha-1 plant density level on the average of the fertilization levels. The crop yields of Enterprise were also the highest at high plant density level (24444 kg ha-1). With the second sowing time the highest yields were obtained at the higher plant density level (65 thousand ha-1) with both hybrids (Jumbo 18978 kg ha-1, Enterprise 18991 kg ha-1), which confirmed the good adaptation capability of these hybrids at high plant density level. In humid crop year with early sowing time the highest yielding hybrid was Enterprise (at 45 thousand ha-1 crop density level 20757 kg-1), at the same time, Jumbo was best yielding at the higher plant density level (18781 kg-1). With the second sowing time the highest crop yield was obtained with Enterprise again (20628 kg ha-1 at 65 thousand ha-1 plant density level). With this sowing time the average yields of Jumbo, was 18914 kg ha-1 respectively. We found that dry crop year and early sowing time provided the best conditions for sweet corn production; the highest yields were obtained under these circumstances, which might be the results of the outstanding water management of chernozem soils. -
Genetic diversity of the Hungarian draft horse assessed by mitochondrial DNA
29-32Views:201Hungarian draft is a horse breed with a recent mixed ancestry. It was developed in the 1920s by crossing local mares with draught horses imported from France and Belgium. To genetically characterize the breed and to set up the basis for a conservation programme, we have employed a molecular marker: a 256-bp D-loop mitochondrial DNA fragment. We analyzed 124 horses representing Hungarian draft horses to assess the maternal phylogeography of the breed. Sequence analysis of a 256-bp segment revealed a total of 34 haplotypes with thirty-four polymorphic sites. High haplotype and nucleotide diversity values (Hd=0.953±0.001; π=0.024±0.001) were detected. The average number of pairwise differences were k=5.998. This breed counts 800 mares today, and only survive due to breeding programmes, this way each haplotype frequency depends on the extent to which mares are involved into the breeding. The reduced number of surviving maternal lineages emphasizes the importance of establishing a conservation plan for this endangered breed. Due to the revealed 34 polymorphic sites we could presuppose twelve maternal linages, which could be a first step for making a breeding programme.
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Determining elements of variety-specific maize production technology
157-161Views:62Our aim was to work out such new maize fertilizer methods and models which can reduce the harmful effects of fertilization, can
maintain the soil fertility and can moderate the yield fluctuation (nowadays 50-60 %).
The soil of our experimental projects was meadow soil. The soil could be characterized by high clay content and pour phosphorus and
medium potassium contents. In the last decade, out of ten years six years were dry and hot in our region. So the importance of crop-rotation
is increasing and we have to strive for using the appropriate crop rotation.
The yields of maize in monoculture crop rotation decreased by 1-3 t ha-1 in each dry year during the experiment (1983, 1990, 1992,
1993, 1994, 1995, 1998, 2000, 2003, and 2007). The most favourable forecrop of maize was wheat, medium was the biculture crop rotation
and the worst crop rotation was the monoculture.
There is a strong correlation between the sowing time and the yield of maize hybrids, but this interactive effect can be modified by the
amount and distribution of precipitation in the vegetation period. At the early sowing time, the grain moistures were 5-12 % lower compared
to the late sowing time and 4-5 % lower compared to the optimum sowing treatment.
There are great differences among the plant density of different maize hybrids. There are hybrids sensitive to higher plant density and
there are hybrids with wide and narrow optimum plant densities.
The agro-ecological optimum fertilizer dosage of hybrids with a longer season (FAO 400-500) was N 30-40 kg ha-1 higher in favourable
years as compared to early hybrids.
We can summarize our results by saying that we have to use hybrid-specific technologies in maize production. In the future, we have to
increase the level of inputs and have to apply the best appropriate hybrids and with respect to the agroecologial conditions, we can better
utilize the genetic yield potential. -
Verifying the farm size – environment interaction
33-41Views:148With this study, based on experience gained in the United States of America, the author intends to draw attention to the fact that farm size related questions in Hungary, which form an integrant part of farm policies all around the world, cannot and should not be discussed without taking environmental relations into account. In the United States, where the excessive use of agrochemicals poses a great threat to the environment, many researchers claim that the conventional large farm model is now getting outdated. These experts question the social and economic benefits of a large farm oriented farm structure and try to convince researchers arriving from Middle- and Eastern-European countries to reform their thought on optimum farm structure. In their opinion, it is essential to let countries in the region understand that they are not required to follow the US course of large farms, which many experts in the States also would prefer to leave. From the facts presented in this study, it can be concluded that large farms by nature can protect the environment less efficiently than small farms. It is also pointed out that the excessive use of fertilizers, which is a trait often attributed to large farms by researchers throughout the world, do not yet apply to large farms in Hungary. As regards the use of pesticides in Hungary, the unfavorable tendency portrayed in foreign literature is identified in this analysis, according to which the larger the farm involved in field cropping the larger per hectare doses they use to protect their crops.
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The effect of Willow extract, Bistep and their combination on some quality parameters of lettuce (Lactuca sativa L.)
239-247Views:148The research described in this paper was conducted at the University of Debrecen, Farm and Regional Research Institute in its Botanical and Exhibition Garden, Hungary. The aim was to investigate the influence of plant biostimulants on the morphological parameters of three lettuce varieties (May King, Kobak and Great Lakes). Willow bark extract and Bistep were used through irrigation and spray onto the plant leaves with the amount of 5% Willow and 0,5% Bistep. Vegetative parameters like head structure (1….10), head weight (g head-1), head closing (1….10), number of leaves, head diameter (cm), internal stem size (cm) and root weight (g head-1) were measured in 2019 and 2020. According to our results, vegetative parameters are significantly affected by plant varieties and treatments. In the experiment of 2019, important vegetative parameters were influenced by the Willow extract, while in the season of 2020 the most influenced parameters were under the impact of Bistep biostimulants followed by Willow+Bistep. However, the most favourable vegetative growth was recorded in the Kobak variety in the season of 2019 followed by May King and Great Lakes varieties in the season of 2020. On the other hand, the interaction effect of plant varieties and plant biostimulants on the vegetative parameters were varied from seasons. For example, for the season 2019, significantly the highest vegetative value was recorded for the interaction of May King variety treated with Willow extract, whereas in 2020, significantly the most influenced vegetative parameters were under the influence of the interaction of Bistep and May King variety followed by the interaction of Willow+Bistep and Kobak variety.
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Phylogenetic studies of Phoma species by maximum likelihood analysis
37-46Views:104The cosmopolitan Phoma genus contains mainly phytopathogenic, opportunistic parasite, and saprophyte fungal species. Up to now the characterization of Phoma species and other taxa of Phoma has so far been determined on the basis of morphology on standardized media, and gene sequence analysis was only used as a confirmative or distinctive complement.
In this study we have tried to study phylogenetic relationships by maximum likelihood method in the Phoma genus. We employed a part of the gene responsible for the synthesis of translation elongation factor 1 subunit alpha protein (tef1) containing both introns and exons, a part of the gene responsible for synthesis of tubulin protein and ITS region containing the internal transcribed spacer regions 1 and 2 and the 5.8S rDNA as potential genetic markers to infer phylogenetic relationships among different Phoma taxa. Twenty-four isolates of eleven different Phoma species were firstly characterised by morphologically, and then their tef1, tubulin and ITS sequences were sequenced and analysed by maximum likelihood method carried out by PAUP*4.0b program. According to constructed phylogenetic trees, the different Phoma taxons are well separated. However these trees do not support the traditional Phoma sections based on morphological characterization.
The maximum likelihood analyses of all three sequences confirmed that the Phyllosticta sojicola species is clustered with the Phoma exigua var. exigua group and the Phoma sojicola is grouped with Phoma pinodella group. The experienced molecular evidences initiate the demand of reclassification of formerly mentioned soybean pathogens.