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Scientific background of precision animal nutrition
95-99Views:105Precision animal nutrition consists of meeting the nutrient requirements of animals as accurately as possible in the interest of a safe, high-quality and efficient production, besides ensuring the lowest possible load on the environment. This is facilitated by electronic feeding based on IT technology, an important but by far not the only tool of precision nutrition. In the present paper the following most important elements of precision nutrition are discussed: diet formulation, quality control of ingredients and compound feeds, reduction of the harmful effects of heat stress in pigs with different nutritional tools, application of the recent findings of the molecular genetics in animal nutrition, the relationship between genetics and animal nutrition and reduction of the N and P excretion by nutritional tools.
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Alternative protein sources in the nutrition of farm animals
21-31Views:374Protein requirement and its demand of farm animals became one of the critical problems in nutrition on a global scale. Protein requirement has been an explicit demand for a long period with soybean meal and animal protein, but recently there are some limitations in relation to their use and the availability of the high quality fishmeal decreases constantly. For this reason there is increased demand for finding new protein sources which could be the alternatives of soybean meal and fishmeal. Alternative protein sources can be divided into seven categories, according to their origin. In different countries, their use depends on the availability in large quantity and at reasonable price.
There is a long tradition of using legume seeds, as alternatives of soybean. Most of them contain some anti-nutritive compounds, but it can be reduced with systematic selection. Oilseed meals are also generally use in poultry and pig nutrition, but those crude protein content varied, depending on the oil extraction technology. Green fodder and leaf protein was also proposed as alternative protein sources, but their use is limited, in particular because of the market price. The amount of bioethanol and starch industry by-products increases gradually in recent years, therefore those became alternatives of soybean meal, or in much less extend, fishmeal. However, amino acid composition of such by-products are far from optimal for poultry and pig; therefore, in the case of their use amino acid supplementation is necessary. Several novel protein sources are proposed in the last decade, such as algae or insect proteins. Recently, their availability and use is limited, but in the near future those would be alternative protein sources in monogastric animal nutrition.
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Study of traditional folk foods from the point of view of nutrition in Hajdú-Bihar County
47-51Views:94Healthy nutrition is one of the major topics of our days. The occurrence of the so called “civilization diseases” is increasing continuously as a result of the changes in our nutritional habits and life-style. It was thought that folk nutrition at the beginning of the 19. Century had several beneficial effects on health. For this reason it was decided to study the food and nutritional habits of the population of Hajdu-Bihar County around the 1930s on the
basis of literary sources to see how well they do match with modern requirements of healthy nutrition. -
Andrological examination of Hungarian Large White and Landrace boars
5-10Views:209The Hungarian Large White and Hungarian Landrace pig breeds have outstanding lifetime performance, production parameters and crossbreeding ability. Nevertheless, during the recent decades, these breeds could not compete with the West European hybrid pigs concerning on production results. In this study, we made a complex andrological evaluation of boars in different Hungarian nucleus breeding stocks. After taking blood and semen samples, performing gonadotropin-releasing hormone (GnRH) challenge, ultrasonic and thermographic examinations were done. Laboratory tests were accomplished in reproduction labs of NARIC ABNMS. Our goal was to determine the reproductive performances of boars and collect samples for future genetic examinations, respectively.
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The effect of agrotechnological factors and the cropping season on sweetcorn (Zea Mays L. convar. saccharata Koern.) production in a humid year
146-151Views:70We have examined the effect of three agrotechnological factors (sowing time, fertilization, crop density) and four genotypes on the yield
of sweetcorn on chernozem soil in the Hajdúság region in 2009. The experiment was set up at the Látókép Research Site of the University of
Debrecen. We have included two sowing times (27 April, 26 May), six nutrition levels (control, N30+PK, N60+PK, N90+PK, N120+PK,
N150+PK) and four genotypes (Jumbo, Enterprise, Prelude, Box-R) at two crop density levels (45 thousand ha-1).
In the humid cropyear of 2010 the amount of precipitation exceeded the 30-year average by 184 mm in the cropping season; the average
temperature exceeded the same by 0.8 C on the average of the examined months. The circumstances were most favourable for sweetcorn
production with the first sowing time, thus, this was when the yield of all hybrids was the highest.
With early sowing time, the highest yield (23437 kg ha-1 yield) was obtained with Enterprise at 45 thousand ha-1 crop density level at
N150 + PK nutrition level. The highest yield of the other three hybrids was 22253 kg ha-1 (Jumbo) 22286 kg ha-1 (Box-R) and 1873 kg ha-1
(Prelude). With the second sowing time, the highest yield was obtained with Enterprise again (22237 kg ha-1) at 65 thousand ha-1 crop
density level. With this sowing time the yield of Jumbo, Box-R and Prelude was 20888 kg ha-1, 17796 kg ha-1 and 17401 kg ha-1, respectively.
We found that the highest yield was obtained at the highest nutrition levels (N120 + PK, N150 +PK) with the first sowing time, while the same
was obtained at lower nutrition levels (N90 + PK, N120 + PK) with the second sowing time. -
Nutrition reaction of different varieties of winter wheat (Triticum aestivum L.) bred in Karcag
63-67Views:139In our study the nutrition reaction of four varieties of winter wheat (KG Bendegúz, KG Kunhalom, KG Kunkapitány, KG Széphalom) has been investigated. In the experiment the effect of twenty different nutrition doses on the yield and thousand kernel weight of the wheat varieties has been studied. Significant difference could be figured out among the yield and thousand kernel weight of the wheat varieties, so the choice of the proper variety determines the quantity of yield. KG Kunkapitány had the highest yield, while KG Széphalom had the highest thousand kernel weight in the experiments.
Investigating the effect of the nutrients on the yield the conclusion was that all nitrogen doses had significant yield increasing effect compared to the untreated plots, but among the 40, 80 and 120 kg ha-1 doses there was no statistical difference. In the case of phosphorus the 40 kg ha-1 dose showed statistically verifiable increase of the yield, while potassium doses have no influence on the yields. The thousand kernel weights reached the maximum values where the highest nutrient doses were applied.
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Variation of boza nutrition parameters based on raw materials
67-70Views:147Boza is a ceral based fermented drink. Raw material of boza could be rise, millet, wheat and rye. Because of the diversity of raw materilas and the method of fermentation boza might show varied quality and nutrition value.
Nowadays healthy and conscious nutrition are playing more and more important roles in our lives. People pay an ever growing attention to the nutrition value of products, such as protein- and sugar content, components with antioxidant properties, and the mineral content of the products. In our research we wanted to know how the protein,-, sugar-, total polifenol-, mikro- and macro element content of products change.
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Carcass and the Meat Quality of Hungarian Lambs
65-71Views:93The author investigated 153 lambs of nine genotypes originating from breeding flocks, and 50 lambs originating from production flocks. The investigations were performed between 1995 and 1998. The authors discuss the evaluation of comformation and fat cover according to EUROP standards. The authors also investigate the proportion of valuable meat by genotype, and – out of the internal value indicators – the dry-matter, protein, fat, connective-tissue and hemin contents comparing the flavour, aroma, tenderness, and oven loss of the different genotypes.
The following findings should be highlighted:
♦ The Hungarian Merino breed should be improved, as – according to EUROP standards, more than 70% of these animals were rated as quality „R”.
♦ Hungarian fattening technology has to be preserved, as the lambs reach the desired slaughter weight within a short period of time, and without over-fattening.
♦ Readiness for slaughter, typical of each genotype, has to be defined, and slaughter at proper weight be achieved.
♦ It has to be re-evaluated whether the Hungarian Merino is the only breed which can be used in Hungary, as none of the investigations really proved the special characteristics and significance of this breed.
The author summarizes the findings of the investigation in five tables. -
Effect of different n-6/n-3 fatty acid proportion oil sources on reproduction performance and fatty acid profile of milk in modern genotype sows - Pleminary results: Preliminary results
121-128Views:127This study was conducted to investigate the effects of supplemental n-6 and n-3 fatty acids on sow’s milk fatty acid composition during the lactation period and on reproductive efficiency of sows in the subsequent gestation period. Data were collected on a total of 213 DanBred sows (108 control and 105 experimental) representing parity of 2–7, respectively. Control and experimental sows were placed in the same housing conditions during lactation and gestation period. Control group received 6.3 g of sunflower oil (SO) per kg feed as n-6 fatty acid supplementation, whilst experimental animals received the same amount of fish oil (FO) as n-3 fatty acid source. Diets were corn meal-extracted soybean meal based. The experiment was conducted in one replication as being a part of a longer and more comprehensive trial. It was found that the consumed long-chain polyunsaturated fatty acids (LC-PUFAs) appeared in the sow’s milk and changed its fatty acid profile. With this alteration, the n-6/n-3 ratio of experimental (FO) sows’ milk were narrower than in SO group (SO: 13.82 vs. FO: 5.89). The benefits of n-3 fatty acids supplementation were evident for the subsequent reproduction cycle, when experimental sows heated more reliable and earlier than control (weaning to oestrus interval: SO: 5.86 vs. FO: 4.48 days). Only 2.33% of experimental sows (FO) did not heat within 7 days after weaning, but this was 12.36% in the control group (SO). The present study requires further research to evaluate the effect of n-3 fatty acids on maintenance of pregnancy and improved subsequent litter size.
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Some aspects of food safety from animal origin
153-155Views:111The relationship between the civilisation illnesses and incorrect nutrition can be considered as demonstrated. The functional foods having one or several nutritional- biological advantage can contribute to the healthy nutrition and to improving the quality of life. Beside the healthy nutrition it is an another important requirement that the food does not contain pathogens or substances of chemical and biological origin namely it has to be safe.
In this publication the foods of animal origin were investigated in the point of view of public health hazards arising during their production and processing.
The contamination and infection of food raw materials, the consequence of chemical substances getting in or developing it the food, the judgement of food deviring from genetically modified or cloned animals are discussed. -
Nutrient deficiency and effects of various nutrition technologies on crop health
109-113Views:204The impacts of climate change on crop production are increasingly noticeable. Extreme weather conditions – such as devastating droughts, which occur more often – have serious effects on crop conditions, thus damaging their defence ability against pathogens and pests. Therefore, in order to achieve high-quality and high yielding crops, it is urgent to elaborate new technologies that improve general condition of crops and prevent development of nutrient diseases. Those crops which suffer from the lack of certain nutrients are more sensitive and their tolerance against diseases are decreased. Nitrogen – as the most influencing macronutrient in yield – is also essential in maintaining crop health. Nevertheless, due to the complicated processes in soil (such as leaching, denitrification), the utilization of nitrogen is not nearly complete, therefore nitrogen stabilizers may be needed to maximize this factor. The use of these stabilizers can be promising where plants with high nitrogen content are grown, although further experiments are needed in which impacts of nitrogen stabilizers on crop protection aspects are examined as well, since there is a close correlation between exaggerated nitrogen fertilizing and sensitivity to pests. During my research I am going to examine the combined effect of foliar fertilizer and nitrogen stabilizer on crop health. Furthermore, my goal is to find clear correlation between pathogens and the different technological variants of nutrition.
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Application of yeasts fortified with microelements – Review
101-106Views:120Microelements are increasingly becoming into the focus of interest from both a point of view of nutrition science and feeding. An always growing care must be paid to the microelements coverage both in human and animal organisms because of incorrect alimentation habits and
unsatisfactory feedstuff nutrition value. For the increased supply of the micro-nutrients, enrichment or fortification with microelements can not only be realized with traditional foodstuffs and forage but there are already alternative ways such as single-cell proteins from yeasts directly enriched or fortified with microelements for the purpose.
We would like to draw the attention that the production of these items is more favourable in comparison with traditional foodstuffs or forage since yeasts are capable to multiply microelement levels compared to their original state, and establish organic bonds with them.
For this purpose, we explored and analysed the scientific literature, studies and research results on this subject, that is why we stressed the significance of yeasts, the features and health effects of certain microelements, as well as the possibilities for use of yeasts enriched with micro-nutrients. -
The effect of water-stress on the mineral nutrition of fruit plantations
187-192Views:83Besides agro-techniques the climatic conditions play an important role in agricultural production. Weather extremes are
significant hazards to many horticultural regions all over the word. It has a profound influence on the growth, development and yields of a
crop, incidence of pests and diseases, water needs and fertilizer requirements in terms of differences in nutrient mobilization due to water
stresses. Nowadays, the weather extremes cause more and more problems and significant hazards to many horticultural regions in Hungary.
The aim of this study is to explore the problems of nutrient uptake followed from climatic anomalies and response it. In this study
we focus on water supply problems (water-stress).
Reviewing the effects and nutrient disorders caused by climatic anomalies, the following statements can be taken:
· Nutrient demand of trees can be supplied only under even worse conditions.
· The most effective weapon against damage of climatic anomalies is preventative action.
· Proper choice of cultivars, species and cultivation should provide further possibilities to avoid and moderate the effects of
climatic anomalies.
· Fruit growing technologies especially nutrition should be corrected and adjusted to the climatic events as modifier factors.
· The role of foliar spraying, mulching and fertigation/irrigation is increasing continuously.
· Urgent task of the near future is to correct and adjust the tested technologies of fruit growing according to these climatic events as
modifier factors.
Optimal nutrient supply of trees decreases the sensitivity for unexpected climatic events. To solve these problems supplementary, foliar
fertilization is recommended, which adjusted to phonological phases of trees. Moreover, mulching is regarded as an excellent water saving
method. -
Possible alternatives in crop nutrition
109-112Views:92The protection of the environment is our common task. All pollution that exposes our soils, plants or the environment – as taken in any proper or extended sense – will appear sooner or later in the food chain and in human beings who are on the top of the food-chain pyramid. The aim of our work is to give a brief overview of the effects of selected industrial wastes on the physiological parameters of corn plants. Sewage sludge and lime sludge were examined. These materials contain lots of useful element for plants (e.g. iron, phosphorus, potassium, zinc). However, their aluminum, chrome and lithium contents are also considerable. The element contents in sewage sludge and the filtrates of lime sludge, as well as the dry matter accumulation and relative chlorophyll contents, were measured. The disadvantageous and advantageous physiological effects of the examined materials were confirmed. The compensation effect of the environment is excluded; however, the neutralization of environmental impacts is not infinite under natural circumstances.
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Fish consumption of the great cormorant in the area of Hortobágy Fish Farm
81-84Views:126The cormorant (Phalacrocorax carbo) is a bird species that nests sporadically but in colonies, besides larger fishponds and rivers in Hungary. The number of its individuals has been increasing during the last two decades. The species eats solely fish, therefore it can cause serious depredation of the fish stocks in fishponds of intensive system and after the freezing of the ponds, in larger rivers, which are not yet frozen. The aim of our research is to reveal the damages the birds can cause on the studied areas and the extent of the losses the Hortobágy Fish Farm Co. has to realize.
Our studies were carried out between April 2012 and November 2013. During cleaning, the investigation of the crop contents of the birds and their biometric studies were conducted. The results revealed the diverse nutrition base of the species. In the samples, we have determined 300 identified fish individuals; in 289 cases, the exact fish species were determined, too. Although statistical differences were found between the given pond unit and the consuming of the corresponding fish species (P=0.05% beside), this cannot be considered as absolutely certain.
Our investigation is of considerable significance in terms of nature preservation and not least, it has substantial financial concerns too. Under the ever harder fish production conditions, the presence and thus the permanent nutrition of the bird affect the fishermen. In favour of the maintaining of the natural ecological balance and the income of the fishers, the elaboration of adequate protective and efficient preventive processes will be necessary in the near future.
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Impact of chronic heat stress on digestibility of nutrients and performance of meat type ducks
51-55Views:188The aim of the study is to determine the effect of vitamin and mineral supplement under permanently high environmental temperature (30±1 °C) on the digestibility of nutrients, performance and furthermore the composition of duck meat in the growing period. A total of four hundred mixed sex 14 days old Cherry Valley type hybrid ducks were used for the study. Two experimental diets were formulated in the study (control and vitamin E, C and zinc supplemented diet). Based on the results the following conclusions were drown: the antioxidant defence system plays an important role in the reduction of heat stress generated lipid peroxidation process. Feed additives which have direct or indirect antioxidant effects can reduce the negative effects of heat stress on the ducks performance and meat composition. Digestibility of nutrients (Dry Matter, Crude Protein, Crude Fat) was not affected by antioxidant supplementation under chronic heat stress (30±1 °C). The performance was affected significantly by Vitamin C and E and zinc supplementation under heat stress (P<0.05). In the treated group the daily weight gain (dWG) increased and the feed conversion ratio (FCR) was improved significantly (P<0.05). The energy and protein conversion was decreased also significantly (P<0.05).
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Comparison of chemical parameters of enzyme active and inactive malt types
161-166Views:199Nowadays there is an increasing emphasis on the use of raw materials. Typically, raw materials – in this study malt – are used in animal feeds and used in the brewing industry. However, in terms of quality (eg. high fibre content), these can be included in human nutrition, we have limited information on this possibility. The aim of our work was to compare different malt flours and examine the possibility of using malt in the baking industry. We were to investigate some of the most relevant parameters, such as dietary fibre content, crude protein content, fat content, carbohydrate content, dry matter content, moisture content, salt and energy content. In the future, we aim to conduct a research on some of these parameters with different malt types as the brewing industry uses novel ingredients different cereals, pseudocereals such as amaranth (Amaranthus spp.), oat (Avena sativa L.), quinoa (Chenopodium quinoa Willd.) in addition to the spring barley (Hordeum vulgare L.) or wheat (Triticum aestivum L.). Based on brewing studies, malt has a high fibre and protein content. Having these advantageous qualities, malt should be part of humans’ healthy diet. Using malt flour in the baking industry can be a new direction which can lead to creating a healthier lifestyle and healthier eating habits than suggested by the WHO (World Health Organization).
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Insect base-protein: A new opportunity in animal nutrition
129-138Views:451The consumption of insect protein, as well as its use in animal feeding, has become a trend. This trend is particularly receiving a lot of attention in animal nutrition because the current protein source of the highest quality in animal feeding which is fishmeal is unsustainable, expensive and its demand is higher than supply. Insect protein can be of great potential in combating world hunger as the world population continues to increase. The potential of insect protein is wide-ranging, it could improve the economics of feed production, lead to cleaner production due to its low environmental footprint.
This review focuses on the need, (nutritional and environmental) advantages of insect protein in feed production, as well as previous research findings.
The unanimous conclusion of the reviewed papers is that insect protein has a great potential in feed production in terms of nutritional value, environmental implication, sustainability as well as economic implication.
However, there is a need for the intensification of research on this alternative protein source in Europe, especially in aquaculture due to the approval of 7 insect species in aquafeeds by the European Union. Another reason for the intensification is that there is still much to be done as a result of past research which shows that growth effects vary based on species and inclusion level.
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The efficiency of the different elements of spring barley growing organic technology in the conditions the eastern steppe of Ukraine
209-213Views:127The effectiveness the use of new nutrient complexes is studied. It is set that a combination of nutrient complexes with organic and organicmineral nutrition background promotes to good growth and development of plants during the growing season, provides the best parameters of crop yield structure and the crop yield and economic efficiency of spring barley cultivation in the condition of the Eastern Steppe of Ukraine.
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The analysis of the milk production and the body condition on two Saanen goat-farms
53-59Views:79The authors carried out an examination on two Saanen goat farms during four months from May to August 2008. The body condition score and milk production of the goats were measured. In data processing analysis of variance was used to compare the data of the studied farms. They tried to find the answer for the question how the body condition scores and milk production change in each month. The changes observed in each farm were examined separately then they compared the values of the two farms.. On one of the farms the nutrition was proper therefore with an ideal, intermediate body condition (average 2.73) the goats produced a good amount of milk (2.5 milk-kg/mother/day). On the other farm the nutrition was not proper, therefore the body condition of the goats was weak (average 1.88). Their milk production decreased significantly from month to month (average 2.35 milk-kg/mother/day). The authors could conclude considering both farms that the more milk the goats produced, the lower their body condition score became. The two parameters showed negative correlation.
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The effect of feeding different glycerol sources on the performance of lactating sows
99-103Views:160Glycerol is a by-product of the biodiesel industry and it might be a good alternative to moderate the energy deficiency of sows during the lactation period. Preliminary experiments were carried out to test the effect of a powder, solid based “food grade” glycerol source with 72.9% glycerol content (Trial 1) and a liquid “feed grade” glycerol source with 86% glycerol content (Trial 2) on the performance of lactating sows and their litters. Trial 1 was conducted with 5 Hungarian Large White×Hungarian Landrace sows/treatment (313±24.9 kg) and Trial 2 with 12–12 DanAvl (323±17.0 kg) sows and their litters/treatment. Neither the solid, powder based glycerol (Trial 1), nor the liquid glycerol source (Trial 2) had significant effect on the feed intake, reduction in live weight and back-fat thickness, and weaning-tooestrus interval (p>0.05) of lactating sows. In Trial 2, on the 14th, 21st and 27th days of lactation the milk samples were collected and it was found that 50 kg/t glycerol decreased the protein content of milk samples (p<0.05). Glycerol supplementation had no effect on dry matter, fat, lactose content of milk samples (p>0.05). In Trial 2, no significant difference was found between control and experimental sow groups in triglyceride concentration of blood samples and in the activity of liver enzymes (ALT, AST, GGT; p>0.05), but the concentration of plasma glucose and cholesterol increased tendentiously (p<0.10).
Based on our preliminary results, it can be concluded that additional dose trials are needed to perform in order to study the effect of glycerol supplement on milk production and on metabolic processes of lactating sows.
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Experiments of sweet potato technology in South Hungary
161-165Views:181The overall objectives of our research program are to examine and to develop the possibilities of use and to expand the national production technology of sweet potato. In 2016, we have set up production technology experiments in many regions, from which we report the results of the experimental field in Deszk here. In our field experiments we obtained results of planting material production, planting and nutrition optimization of sweet potato. Rating the effects of different nutrition doses, between the averages of treatment we didn't experience any significant differencies. On hard soil we set up experimental plots with or without ridges. Based on the result of the harvest the production technology without ridges proved to be more effective. The transplants originating from cuttings from shoots or from tubers did not show significant differences, but it does matter how many tons of sweet potato tubers we harvest per hectare.
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The influence of crop rotation and nutrition regime in winter wheat on the phytomass remains and the deposits of weed seeds remained in the ground
10-13Views:126An important problem refers to soil weeds seed bank with special reference to brown luvic soils and relationship between vegetal remains
(roots + stub) and nutrition regime – crop rotation plant. A great number of seeds can be found in first 20 centimeters of soil (plowing
layer). This fact causes a high weeds density, which has a negative effect on the cultivated plants leading to partial or total crop loss. -
Fertilisation Effect on Quantity of 0.01 M CaCl2 Soluble N-Forms in a Long-Term-Small-Plot Experiment
166-170Views:45long-term experiments have an opportunity to investigate the effects of fertilization and plant nutrition. The paper reports the results achieved in the 39th years of a long-term-small-plot fertilisation and liming experiment set up on acidic sandy brown forest soil in the Nyírség region. From the 32 treatment, four replications, altogether 128 plot experiments with 10 treatments are summarized. We took samples after harvest of triticale, in August.
We used a reliable method (segmented continuous flow analysis) to determine different (easily mobilized - 0.01 M CaCl2 soluble) N-forms of soil. The 0.01 M CaCl2 soluble inorganic and total N content and the UV digestable organic-N form of soil were determined by this method.
The results are summarized below:
– The mineralized-N (Nmin.) content of soil increased with dose of nitrogen treatment. Liming treatments increase the amount of Nmin.
– The maximum content of easily mobilize organic-N-fraction was found in the upper (0-20 cm) layer. This fact due to the large amount of crop and roots.
– Changing of content of 0.01 M CaCl2 soluble total-N-forms due to N doses.
– The ratios of these N forms are variable. It is very important that the content of organic N fraction is not negligible and this fraction plays a main role in the plant nutrition. -
Presence, feeding and health status of collared doves (Streptopelia decaocto Friv. 1838) on animal breeding farms
49-54Views:109In the course of our examinations we were determining the gender of collared doves (Streptopelia decaocto) feeding at animal breeding farms by biometric data. We were estimated their nourishment and necropsies were carried out.
We compared the results of the nutrition analysis with similar data of collard doves feeding at agricultural areas.
We analyzed the crop and gizzard content and recorded the body proportions of collard doves that were captured between December of 2004 and August 2005. In the course of the necropsy examinations we did parasitological, bacteriological examinations as well as examinations for the detection of Newcastle disease. In the course of the gender analysis of the collard doves that were collected at animal breeding farms, both the wing length and the length of the third quill-feather has shown a significantly greater value in the case of males (p>0.05).
From the orts found in the crop and gizzard it has been stated that the feed consumed the most was corn (80.77% m%). The collared doves consumed much less other cereal seeds (wheat 0.82%, barley 1.26%).
In the course of the necropsy examinations done in the Veterinary Institute of Debrecen, the birds proved to be free of parasites, and the results of the Salmonella and Newcastle disease analysis were negative as well. E. coli bacteria had been detected in the liver of not more than three birds.