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  • Evaluation of long term experiments from a new aspect
    55-60
    Views:
    111

    During our work, we developed a new, simple method to show the effects of fertilization on yield, which can both be applied over the long term as well as in series of independent experiments.
    During the testing of this method, at the experimental farm of the Debrecen University Center for Agricultural Sciences at Látókép on a chernozem soil with lime deposits, we examined the fertilizer reaction of maize hybrids between 1989 and 1994. The treatments were: winter tillage, plant density of 70-80 thousand, unfertilized, N 120, N 240 kg/ha fertilized treatments, long term experiments using Dekalb 524 and Volga SC hybrids in long term experiments.
    Four parameters are shown in the model. In the examined period TRmax represents the greatest yield in the fertilized treatments, NT the yield in unfertilized treatment, k the „efficiency of fertilizer” to NT and b the depression-coefficient, where the expected value is zero. The expected grain yield of the fertilized treatments (Y), in the function of the unfertilized grain yield (x) is the following:

    The parameters were determined using the Monte Carlo method, in the optimizing process the sum of deviation square was minimized. The correct conformation of the functions was determined by the greatness of the R-value and the standard error. We found that during six years of testing, the tendency of fertilization efficiency was similar in the case of both hybrids. There was an unfavorable weather interval and, in these years, the yields were low, fertilization did not have an effect and moreover, in extremely bad conditions resulted in an obvious yield decrease. With the  improvement of conditions, which in the case of our country means an increase in precipitation, the efficiency of fertilization increases and reaches its peak at 13-14 t/ha. At this point, the yield increasing effect of fertilization is 4-4,5 t/ha. If the yield of the unfertilized treatments increases from 8-9 t/ha, then the efficiency of the applied fertilizer decreases.
    Most likely, the k and b parameters depend on the soil of the experimental location (nutrient and water management) and on the amount of  pplied fertilizer and the characteristics of the  hybrid. With the increase of fertilizer dosage the k-parameter also increases. The greater value though does not obviously mean a more favorable situation. It is true that in medium and good years this means great fertilizer efficiency, but in low or extreme precipitation conditions it also means greater risk. With the increase of the k-parameter, the yield deviation also increases which, from a cultivation point of view, is quite unfavorable. If the value of the b-parameter is other than, zero then the effect is clearly unfavorable, because with the increase of this value, the yield decrease is also greater. The fertilizer reaction of the two examined hybrids can be well characterized by these two hybrids.
    Examining the six years, our created model estimated the effect of fertilization on the yield accurately and with a high degree of safety. Both in highly unfavorable and extremely good years, it gave an exact estimate. In our opinion, it can be used well to evaluate the effects of fertilization on yield in the future.

  • Compost application in integrated and organic fruit cultivation
    135-139
    Views:
    105

    Nowadays the success vegetable and fruit production are unimaginable without regular nutrient management. One of the ways to supply the required nutrients in an environmentally friendly way is the application of composts, which is less widespread so far. Compost doses were applied in biological and integrated apple orchards in cooperation with the Institute of Horticultural Science in the years 2010 and 2011. Different changes were resulted by the compost treatments in the examined parameters in case of both apple varieties (Golden Delicious and Pinova). There is no clear effect of compost on the changes of ash-, total acids, Vitamin C and the sugar content until now, because the improvement of the nutritional indicators takes more time. However significant differences were observed after one year between the apple varieties. Higher ash-, total acids and sugar content were measured in case of the apple variety Pinova, while the measurements showed higher Vitamin C content in case of the variety Golden Delicious.

  • The effect of supplemental pig milk replacer on carbohydrate metabolism – a review
    67-71
    Views:
    192

    The aim of this review is to examine the effect of milk supplementation on pigs’ carbohydrate metabolism. These parameters regulate the growth of piglets, the nutrient intake and the period of pre-weaning. Due to the increased litter size, the sow milk yield reaches an individual maximum. The individual maximum has not increased proportionally with the number of piglets born alive. The use of liquid milk supplementation may give the opportunity to keep these large litters. The examined hormones are insulin, insulin-like growth factor, growth hormone, growth hormone releasing hormone, ghrelin and leptin. This review seeks to find these hormones’ interactions.

  • Examination of the effect of pelleted poultry manure products on a sunflower test plant in a laboratory model experiment
    83-88
    Views:
    164

    Sunflower (Helianthus annuus L.) is one of the most important oil plants in Hungary, with a sown area of more than 627.000 ha in 2019. Sunflower cultivation is primarily important for use in the oil industry, but its role in feed and beekeeping cannot be neglected either. Sunflower adapts well to different production areas in terms of soil demand, yet, as important fact, it must be grown on soils with poorer conditions with intensive nutrient replenishment. From the agri-environmental point of view, in addition to the use of pelleted poultry manure products, which are also new to the consumer market, is of paramount importance. Their advantages include a wide range of nutrients as well as a soil structure improving effect.

    In our research, we investigated the starter effect of pelleted poultry manure products applied at different doses (200 kg ha-1 and 400 kg ha-1) on sandy soil with humus using a sunflower plant. The aim of our research to investigate the effect of using pelleted fertilizer products as starter fertilizers on sunflower plant germination, and investigate the effect of poultry manure products on soils.

    Based on our results, it can be stated that the pigment content (chlorophyll and carotenoid content) was positively influenced by the higher dose treatments (400 kg ha-1), thus increasing the photosynthetically active leaf area. Compared to the control, the carotenoid content in the test plants of the treatments increased, and the differences of this treatment proved to be significant (p<0.05). Our experiments supported the beneficial effect of pelleted poultry manure-based products in the soil-plant system.

  • Examination of plant number and sowing date in different crop years
    79-82
    Views:
    99

    We examinated three agrotechnical factors in 2011, 2012 and 2013 (sowing time, nutrient factor and plant density), as well as five different effects of genotypes on the crop of corn, on brown soil in the Hajdúság. The experiment was set next to the 47 main road in Debrecen, at the 6th kilometre stone.

    In the present processing I would like to touch on the effects of sowing time and plant density, as I do not have the chance to present the whole experiment results here. It is true for all three years that the humidity factors differ from the long years’ average, so the genotypes had different reactions on it. According to our results we found out that the late sowing time’s result had the most successful crop yield result with 9975 kg ha-1, while examining the plant density the result of the highest plant density proved to be the best with 9967 kg ha-1.

    We take the critical season in corns’ life cycle process into consideration when examining the results: June, July and August months’ humidity and temperature markers. According to the results in the tested 3 months we had 227 mm humidity with 10 days, when the average temperature was over 25 °C. The same factors in 2012 were 135.5 mm humidity with 37 days of average temperatures of 25 °C and in 2013 we recorded 102.5 mm humidity with 24 of these days.

    Our goal is to help the farmers in the Hajdúság with the results of our sowing times, hybrid choice and plant density results.

  • Establishing biotic stress tolerance of maize (Zea mays L.) by measuring hydroxamic acid contents
    107-112
    Views:
    179

     

    Cyclic hydroxamic acids are the most considerable secondary metabolites in grasses and their main task is to protect these species from pathogens and pests. The cyclic hydroxamic acid content and common smut susceptibility were examined in our experiments. 27 maize hybrids were used for experimental plants in a climate room, where the plants were grown on a nutrient solution. An infiltration method was used for the inoculation of the plants. The total quantity of cyclic hydroxamic acids was determined and the ratio of infected plants and the ratio of inhibition was determined, too. Based on our results, on the basis of all hybrids’ data, the total hydroxamic acid content of the infected plants was higher than in the control. On the level of individual hybrids, only 9 of them had higher cyclic hydroxamic acid content in the case of infection. Increase in cyclic hydroxamic content induced by the fungus in this case is a tool for the fungus to suppress other pathogens and pests. Amongst the hybrids’ cyclic hydroxamic acid contents, significant differences were detected in the control and in the infected treatment, too. The so-called “sweetcorn” hybrids showed high level of cyclic hydroxamic acid content. According to the differences amongst hybrids, homogenous groups were created which groups differed in the case of control and infected treatment, because of the difference in increase of cyclic hydroxamic acid content. The examined hybrids showed different levels of infection and different rate of growth inhibition for the effect of inoculation. According to the infection caused damage hybrids were ranked. Infection caused notable damage for hybrids Prelude, Desszert 73, DKC5276 and DK440.

  • Evaluation of the role of common vetch (Vicia sativa L.) green manure in crop rotations
    161-171
    Views:
    128

    Common vetch (Vicia sativa L.) is an annual legume, grown as green manure provide rapid soil cover, can increase soil moisture and organic matter content and reduce soil erosion during fall. During the fallow period, legumes grown as catch crops are known by releasing large amounts of mineral nitrogen (N) for the subsequent crop. By taking advantage of these benefits, it is possible to increase the yield of the next crop in an environmentally friendly and sustainable way. The goal of this study was to determine the value of common vetch as a green manure, considering its effect on soil conditions and the yield of next crops. We examined three different common vetch seed rate as a green manure in a crop rotation with triticale, oat and corn. Next to the green manured treatments, we used fertilized and bare fallow control treatment for comparison. In our study we evaluated the aboveground biomass weight of spring vetch green manure and its effect on the moisture content of the soil. We examined the green manure’s effect on the next crops plant height and yield. We found that the moisture content of the green manured plots was significantly higher during summer drought. On the green manured plots, 37.9% higher triticale yield, 50% higher oat yield and 44% higher corn yield were measured compared to the control plots. The insertion of spring vetch green manuring into crop rotations could be a good alternative to sustainable nutrient replenishment methods. It can be used to reduce the input needs of farming, reduce carbon footprint, contribute to the protection of soils and increase the organic matter content of the soil.

  • Changes in the Macro-, Mezo-, and Microelement Contents of Maize Hybrids in Relation to the Level of Nutrient Supply
    126-130
    Views:
    63

    n my research, I measured the effect of NPK fertilisation on the macro- meso- and microelements content of maize hybrids in 2001. The experiment was set in the demonstration garden of the Department of Crop Production and Applied Ecology in the Agricultural Centre, at the University in Debrecen. The soil of the experiment is calcerous chernozem soil. Five fertilisation steps were applied. Besides the control the smallest rate was 40 kg N; 25 kg P2O5; 30 kg K2O of active ingredients. The largest rate was five times more than the smallest one: 200 kg N; 125 kg P2O5; 150 kg K2O, which is equal to 475 kg mixed active ingredients. The NPK treatment significantly influenced the macrolement content in several cases. The N content was the lowest in the control treatment. Compared to this the fertiliser treatments significantly increased the N content of hybrids. However the highest amounts of potassium and phosphorus could be measured in the control and the lowest amounts could be measured at the N 200+PK kg/ha treatment.
    The Ca content of hybrids was the highest in the N 120+PK kg/ha treatment, while their Mg and Zn content was the highest in the control treatment. The lowest amounts were weighed in the N 200+PK kg/ha treatments, that in several cases resulted in statistically proved decreasement compared to the control or the lower fertilizer doses. Considering the two mesoelements and Zn the most favourable results were obtained in the case of the Norma SC and DK 366 SC hybrids.
    Summing up what has been said moderate amounts of fertiliser doses (N 40-120+PK kg/ha) had a favourable influence on the micro- and macroelement content of hybrids.

  • Interactive evaluation of the main agrotechnical factors in rape production
    71-79
    Views:
    76

    Our polifactorial rape research was carried out at Látókép Research Centre of Debreceni Egyetem AMTC, 15 km away from Debrecen. The aim was to study the unique effect and the interactive effect of more factors. The research factors were the following: cultivation, time of sowing and nutrient supply. Soil moisture datas proved unambiguously that increasing amounts of chemical fertilizer raise the water consumption of rape, lack of water in fertilized plots were always bigger then the water deficit in control plots. The highest amount of water deficit was experienced in the case of arable plots. However, increasing amounts of chemical fertilizers raised the amount of yield proportionately. We experienced yield depression only in the case of arable plots at the highest level of chemical fertilization. In polifactorial rape research sowing of 24th August 2007 of 2007/2008 was the most optimal in point of the amount of yield. This is most-significant in the case of loosening tillage and disking tillage plots, while the plots of ploughing lag behind those two in point of average yield. We experienced the biggest differences of yield in the case of different tillage plots of sowing on 24t August 2007. Still not even the plot with the highest average reached the limit of 4 tons, which can be attributed to high rate of lodging and the harvest loss caused by this. The biggest amount of yield was experienced in the case of sowing of 24th August 2007, with the highest level of chemical fertilization at loosening tillage plot (3930 kg/ha). We can observe big differences between the tillage methods; plots of loosening show a much better average yield then plots of disking and ploughing tillage. Considering the first observed crop year we can state that alternative tillage methods do have a future in rape growing of Hungary. 

  • Describing Fusarium diseases on maize in 2013 using data from several production sites
    60-64
    Views:
    137

    As in other parts of the world, the frequency of weather extremes has increased greatly in Hungary in recent years. This means that maize production is faced with greater risks from all aspects: nutrient replacement, irrigation, plant protection. This is especially true of fusarium diseases. In a continental climate, the pathogens causing the most serious problems are species belonging to the Fusarium genus. They infect the ears, which – besides reducing the yield – poses considerable risk to both human and animal health due to the mycotoxins produced by them. Depending on which Fusarium species are dominant at a given location, changes can be expected in the level of infection and in the quality deterioration caused by the mycotoxins they produce. Fusarium spp. not only damages the maize ears but when pathogen attacks the stalk, the plant dies earlier, reducing grain filling and resulting in small, light ears. In addition, the stalks break or lodge, resulting in further yield losses from ears that cannot be harvested. The degree of infection is fundamentally determined by the resistance traits of the maize hybrids, but also a great role in that region Fusarium species composition as well.

  • Study of plant physiological parameters in winter oilseed rape (Brassica napus var. napus f. biennis L.) production on chernozem soil
    111-115
    Views:
    185

    We made plant physiology examinations in Arkaso winter oilseed rape hybrid substance: relative chlorophyll content (SPAD) and leaf area index (LAI) measurements. The experiment was set in University of Debrecen Agricultural Sciences Center at Látóképi Experimental Station in four replications, in two different sowing times (I. sowing date on 08/22/2014 and II. sowing date on 09/09/2014 sowing againhappened because of the incomplete germination in the second subtance 01/10/2014) Three different plant density 200, 350 and 500 thousand ha-1, under the same nutrient supply, 45 cm row spacing. The experiment was green crop of winter wheat. The relative chlorophyll content (SPAD) and leaf area index (LAI) measurements were made in seven different times. We measured the maximum value of chlorophyll content in the first sowing time at 500, and the second sowing time at 350 thousand ha-1 plant density. The measurement results proved that there was a linear relationship between the number of plants and the LAI. The maximum leaf area index values we measured in both the sowing time at 500 thousand ha-1 reached.

  • Grain yield and quality of maize hybrids in different FAO maturity groups
    126-131
    Views:
    63

    An improvement in the quality of maize grain by increasing the level of components responsible for its biological value is possible
    by using genetic means. However, a change in the genotype, together with improving the nutrient properties of the grain, also has some
    adverse consequences connected with a fall in yield and in resistance to diseases.
    Field experiments were conducted during three years (2003, 2004and 2005) to evaluate environmental effects on grain yield and
    quality responses of maize hybrids. Twenty one hybrids of various maturity groups (FAO 150-400) were planted to achieve an optimum
    (60-70 000 plants per hectare) plant populations and grown under the medium-N (80 kg N ha-1) fertilization. Environmental conditions
    significantly affected maize hybrid responses for grain yield, starch, oil and protein contents, and consequently, starch, oil and protein
    yields per hectare. Hybrids of flint type, which have a short vegetation period, had high protein and oil content but the yield averages
    were low due to the slower rate of starch incorporation. Hybrids of the dent type have a longer growing season and more intense
    carbohydrate accumulation, but low protein and oil contents. In wet years there was a higher rate of starch accumulation, while dry
    years are favorable for protein and oil accumulation. Positive correlation existed between starch content and grain yield and 1000-
    weight as well as between oil content and volumetric weight among tested hybrids. Negatively correlation existed between grain oil and
    starch content as well as between oil content and grain yield and 1000-weight. Thus, end-users that require high quality maize may need
    to provide incentives to growers to off set the negative correlation of grain yield with oil and protein content.

  • Advancement of a Common Wheat (Triticum aestivum L.) Selection System
    8-11
    Views:
    82

    All the research in Hungary and other countries in Europe focus on improving the quality of crops and increasing the competitiveness of production.
    In this respect, we have to advance the conventional technological elements, reduce the application of pesticides and fertilizers, and produce new varieties suitable for environmentally-sound production. In our crop breeding programs, we applied conventional and biotechnological methods (embryo rescue and double haploid methods) in order to get somaclonal and gametoclonal variations.
    We produced winter wheat lines (HP-31-95, HP-82-96) by traditional way, which have high baking qualities and high nutrient efficiency. Some diseases can limit the quantity and quality of a wheat crop. We examined several wheat diseases in our winter wheat candidates, and we found that our progenies have resistance to leaf rust.
    With respect to a serious problem was the small quantity of applied fertilizers and the other externals, our research focused onto advance a common wheat selection system, with the help of it, we can handle these problems. We have summarized that our selected progenies can compete with the registered varieties with quality and environmental respect.

  • Environment-friendly nutrient supply in the area of NYÍRERDŐ Zrt
    111-116
    Views:
    111

    The widespread realization of environmental friendly management is an important segment of seeking sustainable technological and
    technical solutions. The methods ensuring sustainable development by exploiting natural facilities have great significance. Bacteria-based
    biofertilizers and wood ash are good examples of them. Using bacteria-based biofertilizer means a kind of biofertilization that is using the
    possibilities provided by the ecosystem.

  • Establishing regional cultivating districts on the basis of the Kreybig practical soil mapping system
    20-25
    Views:
    86

    With the help of this report evaluating the current situation of the region, characteristics of the development in agricultural production and regional differences can be clarified. By mapping out the regional soil, land use and climatic conditions and organizing these into a geographical information system, one can easily determine which plants are the most ideal to cultivate in that particular region. Moreover, it is a useful tool that enables us to
    establish the most favorable land use structure suited to ecological demands and also helps to determine the methods of soil protection.
    During our work, we chose administrative units in Szabolcs-Szatmár-Bereg County, based on the latest aspects of regional cultivation.
    Our pilot areas are: the small regions of Nyíregyháza, Nyírbátor, Nagykálló, Mátészalka and Csenger.
    Using the database, we separated and uncovered the soil conditions of the pilot areas: the chemical and physical properties of the soil layer which is exploited by the roots of the plants, the humus content, the nutrient supply, the thickness of the cultivated layer and the water management conditions.
    We separated the districts of regional cultivation, where the basic elements of the traditional Kreybig color systems were applied (light yellow, dark yellow, light brown, dark green, blue, pink, red, gray, greenish brown, reddish purple, light purple, dark purple, light green).
    By using the data collected from the pilot areas, we compiled a map database, which is suitable to illustrate the plant cultivating characteristics of the region. We made recommendations to determine the most favorable plants to cultivate in the specific region with the given meteorological and soil conditions, as well as for the shifting of crops.
    Our recommendations were also illustrated in a map with a resolution of 1:25000. 

  • The effect of nickel-contamination, nitrogen-supply and liming on the chemical composition of perennial ryegrass (Lolium perenne L.)
    85-92
    Views:
    90

    Plant-production is determined by many production-factors. Each of these factors became subject of research-works through the years, still we state, that studying their interaction is even more important. For studying these interactions we set up a potexperiment, within that the direct effect and the interactions of four factors was inspected: soil, nitrogen-supply, nickel-loading and liming. Experiments were carried out on two soil types with extremely different characters: one was a chernozem soil with good fertility and buffering capacity, the other was a shifting sand soil with low humus-content and buffering capacity. Nitrogensupply and liming was added on two levels, while nickel on three within 12 combinations on each soil types. Plant production was cut two times within the vegetation period. The amount of production and dry matter was weighted, fractured and their element-content was measured by an ICP-detector.
    Ca-content on the shifting sand soil was determined by all three factors, however the interaction between nickel-loading and liming was also significant. Nitrogen and liming increased Cauptake, that is due to appropriate nutrient-supply and improvement of a better pH-value. On the chernozem soil nitrogen and CaCO3 also increased the Ca-content. This is caused by a better nutrient supply and a higher amount of available Ca-ions.
    On the shifting sand soil nickel content was increasing parallel to higher nitrogen-dosages. In presence of higher nickelamount the nickel-content of plants was also increasing, still according to liming, this increment was different. On the chernozem soil nitrogen a nickel increased Ni-uptake. However, liming also had a positive effect on Ni-content, that can be explained by the high amount on colloids in the soil, the adsorption of Ni-ions on them and in presence of liming material the replacement of Ca-and Ni-ions. 
    The potassium-content on the shifting sand soil was different in each liming-combination. In combinations without nickel the potassium-content of limed and not limed combinations was on the same level. In not limed combinations by adding nickel potassiumcontent was increasing, while in limed combinations no change was observed. On the chernozem soil by adding liming material the amount of uptaken potassium was decreasing, that is due to the antagonism between Ca- and K-ions. 

  • The effect of diet composition, a probiotic and a symbiotic treatment on the ileal microbiota composition of one-week-old broiler chickens
    213-220
    Views:
    125

    A healthy microbiota present in the small intestine contributes significantly to small intestinal function, including digestion, nutrient absorption and health. The current study investigated the effects of a prebiotic, a probiotic and a symbiotic supplementation on ileal microbiota composition of broilers at 7 days of age. In a total of 574 male Ross 308 day-old chickens were divided into four groups using six replicate pens and 24 chickens per pen. A maise-soybean based control diet (C), a control diet supplemented with probiotics (Broilact; Br), a control diet supplemented with symbiotic (inulin, yeast, Bacillus subtilis; Sy) and a wheat based diet supplemented with wheat bran (W) were formulated. On day 7 of life, two chickens per pen were slaughtered and ileal chymus samples were collected. For microbiota analysis 16S rRNA (V3-V4 region) gene targeted Illumina MiSeq sequencing was used. Feeding all diets supplemented increased the diversity to varying degrees compared to the control (C) diet (p=0.006). As a conclusion, all supplementation substantially influenced ileal microbiota of broiler chickens at an early age. All these results could offer some information for the future study on the relationship between early intestinal microbiota and the compounds of the feed.

  • Changes in weed flora of basket willow (Salix viminalis L.) under different soil nutrient supply
    116-120
    Views:
    127

    The world is in a continuous progress, as a result of which energy consumption and with this the release of gases with adverse impact show rapid increase. As a result of the survey conducted by the International Energy Agency, if the major economic powers do not initiate a change in their energy policy, the increase of energy consumption may as well reach 40 % by 2030. This increased energy demand is getting more and more difficult to fulfill with the fossil energy resources, which is to lead to an increasing significance of renewable energy resources. In Hungary, these energy resources are the best to provide with biomass growth. Biomass growth for energetic purpose can mostly be provided by energy plants, out of which “energy willow” (Salix viminalis L.) is outstanding with its high yield and with its excellent burning technology characteristics of its timber. The willow’s cropping technology is being established in our country. One of our tasks is to work out an adequate weed control plan. The professional and safe use of herbicides can increase the success of production. In our paper, we discuss the weed flora data collected on  treatments applied in the different fertilizer and compost. We started our survey in 2010. We examined twelve different fertilizer and compost treated areas. The dominant weeds were: Amaranthus retroflexus, Chenopodium album, Echinochloa crus-galli among annuals; Cirsium arvense and Agropyron repens among the perennials. 

  • The effect of biopreparations in pot experiment
    45-49
    Views:
    129

    In pot experiment the effect of Amykor and Organic Green Gold bioproducts and their combinations with NPK fertilizer on some soil properties (chemical parameters) and on the biomass of testplant were studied. The experiment was set up in 2012 at the Institute of Agricultural Chemistry and Soil Science, in a three replications, in a random block design. The studied soil typein the pot experiment was humus sandy soil from Debrecen-Pallag with onion (Allium cepa) test plant. At the end of the experiment (after 4 week) in our laboratory the samples of soil and plant were determined. The nitrate-nitrogen, AL-soluble phosphorus and potassium content of soil, the weight of green onion leaves, the wet weight of bulb and root of onion and biomass of onion. The results of the study were the following: – The treatments influenced positively the nitrate-nitrogen, the AL-soluble phosphorus and potassium content of soil. – The most effective treatments were the artificial fertilization (NO3-N) and the NPK+ simple dose of Amykor (AL-P2O5 and Al-K2O). – The NPK fertilization and the NPK+OGG (sprinkle in every 10 days) combinations had significant positive effect on the weight of green onion leaves. – The biofertilization and the straw+biofertilizer combinations stimulated the AL-soluble potassium content of soil occasionally. – The OGG treatment (sprinkle in every 10 days) had significant effective impact on the wet weight of bulb and root of onion. – The biomass of onion was increased by the artificial fertilization and OGG (sprinkle in every 10 days) treatment.