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The effect of lead and copper heavy metal salts on soil microorganisms under laboratory circumstances
55-59Views:137he population dynamics of calcareous chernozem soils polluted with different concentrations of lead and copper heavy metal saline solutions was examined.
The experiment was carried out in the soil biological laboratory of the Institute of Agricultiral Chemistry and Soil Science at DE AGTC MÉK in 2012. For the determination of the concentration of the undiluted stock solutions we multiplied the smallest toxic concentration values of the MSZ 08-1721/1-86 Hungarian standard by forty. The intermediary concentrations of the treatments were produced with adequate dilution of the stock solutions until a dilution level equal to the values of the standard. The statistical evaluation of the data was performed with ANOVA (Analysis of Variance) including the determination of the standard deviation and significant difference. Investigating the effects of the different treatments on the soil microbes we established that both heavy metal saline solutions had a negative effect on the population dynamics of bacteria and microscopic fungi living in the soils. The negative effect of copper – as a potential toxic micro nutrient – turned out to be less strong than the negative effect of the toxic lead. According to our results the correction of the treatment levels is recommended in order to further tolerance examinations and the determination of the tolerance levels.
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Effect of PRPSOL soil conditioner on the physical status of the soil in conventional and reduced tillage systems
109-113Views:194The effect of PRP-SOL soil conditional on soil compaction, moisture content and bulk density is studied in a long-term soil cultivation experiment from 1997 on a heavy textured meadow chernozem soil, in reduced and conventional tillage at Karcag Research Institute. Our investigations were made in the vegetation period of corn, in June and after harvesting, on stubble. Soil compaction was measured with a penetrometer, the actual moisture content was determined by gravimetric method. The bulk density values of the regularly cultivated soil layer of 0–10 and 10–20 cm depths were defined from undisturbed soil samples. We established that after 3 years the application of the soil conditioner has positive effect on soil compaction and moisture status of the top layer in the reduced tillage system. We could not figure out this positive effect in the case of conventional tillage.
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Evaluation of long term experiments from a new aspect
55-60Views:112During our work, we developed a new, simple method to show the effects of fertilization on yield, which can both be applied over the long term as well as in series of independent experiments.
During the testing of this method, at the experimental farm of the Debrecen University Center for Agricultural Sciences at Látókép on a chernozem soil with lime deposits, we examined the fertilizer reaction of maize hybrids between 1989 and 1994. The treatments were: winter tillage, plant density of 70-80 thousand, unfertilized, N 120, N 240 kg/ha fertilized treatments, long term experiments using Dekalb 524 and Volga SC hybrids in long term experiments.
Four parameters are shown in the model. In the examined period TRmax represents the greatest yield in the fertilized treatments, NT the yield in unfertilized treatment, k the „efficiency of fertilizer” to NT and b the depression-coefficient, where the expected value is zero. The expected grain yield of the fertilized treatments (Y), in the function of the unfertilized grain yield (x) is the following:
The parameters were determined using the Monte Carlo method, in the optimizing process the sum of deviation square was minimized. The correct conformation of the functions was determined by the greatness of the R-value and the standard error. We found that during six years of testing, the tendency of fertilization efficiency was similar in the case of both hybrids. There was an unfavorable weather interval and, in these years, the yields were low, fertilization did not have an effect and moreover, in extremely bad conditions resulted in an obvious yield decrease. With the improvement of conditions, which in the case of our country means an increase in precipitation, the efficiency of fertilization increases and reaches its peak at 13-14 t/ha. At this point, the yield increasing effect of fertilization is 4-4,5 t/ha. If the yield of the unfertilized treatments increases from 8-9 t/ha, then the efficiency of the applied fertilizer decreases.
Most likely, the k and b parameters depend on the soil of the experimental location (nutrient and water management) and on the amount of pplied fertilizer and the characteristics of the hybrid. With the increase of fertilizer dosage the k-parameter also increases. The greater value though does not obviously mean a more favorable situation. It is true that in medium and good years this means great fertilizer efficiency, but in low or extreme precipitation conditions it also means greater risk. With the increase of the k-parameter, the yield deviation also increases which, from a cultivation point of view, is quite unfavorable. If the value of the b-parameter is other than, zero then the effect is clearly unfavorable, because with the increase of this value, the yield decrease is also greater. The fertilizer reaction of the two examined hybrids can be well characterized by these two hybrids.
Examining the six years, our created model estimated the effect of fertilization on the yield accurately and with a high degree of safety. Both in highly unfavorable and extremely good years, it gave an exact estimate. In our opinion, it can be used well to evaluate the effects of fertilization on yield in the future. -
Agronomic research in Martonvásár, aimed at promoting the efficiency of field crop production
89-93Views:115The effect of crop production factors on the grain yield was analysed on the basis of three-factorial experiments laid out in a split-split-plot design. In the case of maize the studies were made as part of a long-term experiment set up in 1980 on chernozem soil with forest residues, well supplied with N and very well with PK. The effects of five N levels in the main plots and four sowing dates in the subplots were compared in terms of the performance of four medium early hybrids (FAO 200). In the technological adaptation experiments carried out with durum wheat, the N supplies were moderate (2010) or good (2011), while the P and K supplies were good or very good in both years. Six N top-dressing treatments were applied in the main plots and five plant protection treatments in the subplots to test the responses of three varieties.
The results were evaluated using analysis of variance, while correlations between the variables were detected using regression analysis.
The effect of the tested factors on the grain yield was significant in the three-factorial maize experiment despite the annual fluctuations, reflected in extremely variable environmental means. During the given period the effect of N fertilisation surpassed that of the sowing date and the genotype. Regression analysis on the N responses for various sowing dates showed that maize sown in the middle 10 days of April gave the highest yield, but the N rates required to achieve maximum values declined as sowing was delayed.
In the very wet year, the yield of durum wheat was influenced to the greatest extent by the plant protection treatments, while N supplies and the choice of variety were of approximately the same importance. In the favourable year the yielding ability was determined by topdressing and the importance of plant protection dropped to half, while no significant difference could be detected between the tested varieties. According to the results of regression analysis, the positive effect of plant protection could not be substituted by an increase in the N rate in either year. The achievement of higher yields was only possible by a joint intensification of plant protection and N fertilisation. Nevertheless, the use of more efficient chemicals led to a slightly, though not significantly, higher yield, with a lower N requirement. -
A tárolási feltételek hatása a kukorica Fusarium fertőzöttségére és toxin szennyezettségére
28-32Views:194Corn samples harvested in 1997, 1998 and 1999 from different soil types were stored at different conditions (temperature, moisture content, state of kernels) for six months. The Fusarium contamination was examined by plate dilution method and the amount of mycotoxins (F-2, T-2, HT-2, DON, DAS) were determined applying HPLC method immediately after harvesting and in the third and sixth month of storage. The aim of our study was to find correlation between the ecological factors, storage conditions and the examined parameters mentioned above, as well as to prove them statistically. According to the examinations carried out after harvesting we could state that the soil type had no effect on the parameters. Analysing the effect of the years we found considerable differences. The Fusarium infection of corn samples in 1998, while the toxin contamination in 1999 was the highest. The results of storage experiment show that year (number of microscopic fungi, F-2, T-2, HT-2, DAS and total toxin content) and moisture content of kernels (F-2, T-2, and total toxin content) have a significant effect on the examined parameters. We could prove the effect of temperature on the T-2 content (samples with natural moisture content) and DON content (samples with 14% moisture content). Higher values were found at higher temperature storage. The ratio of damaged kernels influenced the DON concentration in the non- wetted samples and the number of microscopic fungi in the wetted samples.
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Effect of Irrigation on the Yield and Quantity of Potato Varieties
53-61Views:86In Hungary, the growing area of potato area dropped dramatically in the last few decades. Additionally not only are we lagging behind Western European countries as regards yields, but the competitiveness of production is further decreased by the great alternation in yields from year to year, unpredictable market conditions, poor consumption habits and, often the lack of quality products.
The experiment was carried out at the experimental site of the University of Debrecen, Farm and Regional Research Institute, at Látókép. In our experiment, we examined the yield and some quality parameters of 9 medium-early varieties in large parcels. Of the examined varieties, 3 are of Dutch, and 6 are of Hungarian breeding.
The experiment was set up in 2003 and 2004, in two years of significantly different precipitation, on 50 m2 parcels on calcareous chernozem soil after winter wheat as a forecrop in both years. The 9 varieties were examined in 4 repetitions in randomized blocks, from which two repetitions were irrigated, and two were non-irrigated.
We examined the yields of the varieties, the distribution of tubers according to size and their percentages, and the changes in specific parameters of quality and inner content due to irrigation. We studied the dry matter content, the starch content, the underwater mass, the amount of reducing sugars, and the colour index of frying of the tubers.
In Summary, it can be stated that among the agrotechniques, year effect, variety and irrigation factors have considerable impact on potato yield quality and quantity. However the effect of irrigation depends on the crop year. In a draughty year, like in 2003, irrigation could increase the yield by 10%, while in a more favourable wet year, the improving effect of irrigation was low. -
Comparative examination of a mineral fertiliser and a bacterial fertilizer on humic sandy soil
111-116Views:79In our pot experiment, the impact of a bacterial fertilizer, Bactofil® A10 and a mineral fertilizer Ca(NO3)2 applied in different rates was studied on some soil chemical and microbiological characteristics of a humic sandy soil (Pallag). Perennial rye-grass (Lolium perenne L.) was used as a test-plant. Samples were collected four and eight weeks after sowing in each year. The experiment was set up in 2007-2009 in the greenhouse of
the UD CASE Department of Agrochemistry and Soil Science. The available (AL-extractable) nutrient contents of soil, among the microbial parameters the total number of bacteria, the number of microscopic fungi, cellulose-decomposing and nitrifying bacteria, the sacharase and urease enzyme activity, as well as the soil respiration rate were measured.
Statistical analyses were made by means of the measurements deviation, LSD values at the P=0.05 level and correlation coefficients were calculated. Results of our experiment were summarised as follows:
− The readily available nutrient content of humic sandy soil increased as affected by the treatments, in case of the available (AL-extractable) phosphorus and potassium content the higher value was measured in high-dosage artificial fertilizer treatment.
− The treatments had also positive effect on several soil microbial parameters studied. The higher-dosage mineral fertilizer treatments had a beneficial effect on the total number of bacteria, cellulose-decomposing and nitrifying bacteria. No significant differences were obtained between the effect of treatment in case of the total-number of bacteria, the number of microscopic fungi and nitrifying bacteria.
− On the sacharase enzyme activity the artificial fertiliser treatments proved to be unambiguously stimulating, the urease activity significantly increased on the effect of the lower-dosage Ca(NO3)2 artificial fertilizer treatment.
− The soil respiration increased in all treatments in related to the amounts applied, significantly increased in the highest rate of Ca(NO3)2 fertilizer addition.
− Some medium and tight positive correlations were observed between the soil chemical and microbiological parameters studied in case of both nutrient sources.
Summarizing our results, it was established that the organic and all the mineral fertilizer treatments had beneficial effects on the major soil characteristics from the aspect of nutrient supply. In majority of the examined soil parameters (AL-extractable phosphorus- and potassium, total number of bacteria, number of cellulose-decomposing and nitrifying bacteria, activity of sacharase enzyme) the high rate of Ca(NO3)2 mineral fertilizer treatment proved to be more stimulating, but at the same time the high rate bacterium fertilizer resulted in significant increases in
the nitrate-N content, the AL-potassium content of soil, the total number of bacteria, the number of cellulose-decomposing and nitrifying bacteria and the urease enyme activity.
Our examinations showed that the mineral fertilizer treatments proved to be more stimulating on most of the soil parameters studied but according to our results, it was established that Bactofil is efficiently applicable in the maintenance of soil fertility and the combined application of
mineral fertilizer and bacterium fertilizer may be a favourable opportunity – also in aspect of the environmental protection – in maintaining soil fertility. -
Antimicrobial effect of dried sage on the microbiological state of fresh Hungarian sausage
189-192Views:109The purpose of this study was to evaluate the microbial effect of dried sage (Salvia officinalis L.) on the traditional Hungarian sausage. We added 0.5, 1, 1.5, and 2 w/w% of sage to the sausages and tested them on the 0th, 7th and 14th day. The added dried sage had no effect on the tested microorganisms, but the sage extract inhibited Salmonella, Enterococcus faecium and Staphylococcus aureus.
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Effect of Moringa leaf extract and set size on the bulb weight, diameter and yield of onions (Allium cepa L.)
127-131Views:229Studying the use of natural extracts or biostimulants in improving vegetable plants is the current needs as an alternative way to the use of chemical products. This experiment was carried out to evaluate the effect of Moringa oleifera leaf extract (MLE) as a natural growth regulator on bulb diameter, weight and yield of onions ‘Texas Early White’ cultivar. Therefore, three concentrations (control, 2%, 4% and 6%) of MLE and three different onion set sizes [small (3±1 g/set), medium (6±1 g/set), and large (10±1 g/set)] were prepared in a randomized complete block design with three replications and plant spacing 20×15 cm. MLE was applied as a foliar spray directly onto the plants with the amount of 25 ml/plant every two weeks from sprouting to maturity. The achieved results indicate that the interaction of foliar spray with moringa leaf extract 4% and large set size of 10±1 g/set gave the highest value of the total yield 4802.7 g m-2, while the lowest yield 1531 g m-2 was recorded in the interaction of control and the smallest set size of 3±1 g/set. Also, a significant difference was recorded in bulb diameter separately. Thus, the largest bulb diameter 68.90 mm was obtained from the planting of the largest set size 10±1 g/set with 4% of MLE while the smallest bulb diameter 38.40 mm was recorded from the smallest set size 3 ±1 g/set with control treatment. Similarly, a significant effect was recorded in both set size and MLE separately. The highest bulb diameter 58.71 mm was achieved in the planting of the largest sets 10±1 g/set, while the lowest diameter was 42.12 mm in the planting of the smallest set size 3 ±1 g/set. Similar results were recorded for the bulb weight and total yield. The concentration of 4% MLE produced the highest bulb weight 99.74 g/bulb and yield 3324.5 g m-2, while the lowest bulb weight and yield was obtained in the control treatment 55.61 g/bulb, and 1869.3 g m-2. Consequently, the results indicate the positive effect of Moringa leaf extract which can be applied as a natural substance in the form of foliar spray at critical growth stages to improve the growth and yield of dry onions.
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The impact of different fertilization methods on some microbiological soil characteristics
119-126Views:93In our experiment, we studied the impact of an organic fertilizer, Bactofil® A10 (half- and full dosage applied in field practice) and an artificial fertilizer of Ca(NO3)2 content in different dosages (20-40 mg kg-1) – in addition to control treatments – on two different soils (calcareous chernozem, humus sandy soil) in 2005-2006, the experiment was complemented with treatments applying 250% dosage (100 mg kg-1 N, Bactofil® A10 2.5 times the field dosage) and a compost from urban sewage (25 g kg-1 compost) was also tested on these two soil types. In the
experiment, several soil microbial parameters were studied. The experiment was set up at the Department of Agrochemistry and Soil Science using 1-kg pots.
Our laboratory experiments were performed at the soil microbiology laboratory of UD CAS Department of Agrochemistry and Soil Science, the total number of bacteria, microscopic fungi, nitrifying and aerob cellulose-decomposing bacteria were determined together with the CO2-production of soil, N content of the biomass and urease enzyme activity.
Statistical analysis of the data was done using the program SPSS 13.0, means of the measurements, deviation and significance values were calculated.
In 2005-2006, the effect of the different dosages of Bactofil® A10, and the Ca(NO3)2 fertilizer on the examined microbial parameters of calcareous chernozem and humus sandy soils can be summarized as follows:
• Concerning the total number of bacteria, both treatments were effective on calcareous chernozem soil, the higher (significant) increment in bacteria number was observed in the artificial fertilizer treatments, while in the humus sandy soil Bactofil treatments had a beneficial effect. The number of microscopic fungi also increased in both treatments, higher numbers were observed in the average of two years in the Bactofil treatments.
• The number of nitrifying bacteria was 2.5 times higher in both high-dosage treatments on calcareous chernozem soil, while on humus sandy soil a slight (not significant) increment was observed only int he high-dosage Bactofil treatment. The amount of aerob cellulose-decomposing bacteria significantly increased on calcareous chernozem soil in both the highdosage artificial fertilizer and the small-dosage Bactofil treatment, however, on humus sandy soil no significant increase was observed in either treatment.
• The CO2-production increased in both soil types, although it was not significant in either treatment. A higher (though not significant) soil respiration was observed in the Bactofil treatments in both soil types.
• The microbial biomass N values were significantly higher in the high-dosage Bactofil treatments, however, the high-dosage artificial fertilizer treatment also increased these values significantly on calcareous chernozem soil.
• On calcareous chernozem soil, urease activity was significantly increased and reduced by high-dosage artificial fertilizer treatments and Bactofil treatments, respectively. On humus sandy soil, urease activity was also reduced except for the high-dosage artificial fertilizer treatment. In 2007, the pot experiment with 250% dosages was complemented with the application of compost rich in organic matter, the results of these treatments are sumnmarized as follows:
• In the case of the total number of bacteria, all three treatments resulted in a significant increase on calcareous chernozem soil with the highest values in the Bactofil treatment. The Bactofil treatment was the most effective on the humus sandy soil, but the artificial fertilizer treatment also
resulted in a significant increment. In the case of the total number of fungi, Bactofil treatments resulted in the highest values on both soils, but the compost treatment also increased the number of fungi in calcareous chernozem significantly.
• The number of nitrifying bacteria was increased most (significantly) by the Bactofil and compost treatments on both soil types. The amount of cellulose-decomposing bacteria was significantly increased by he compost treatment on calcareous chernozem soil, while its effect was not significant on humus sandy soil. The number of these bacteria was increased significantly by the Bactofil treatment on humus sandy soil.
• On calcareous chernozem soil, all three treatments significantly increased CO2-production, while the compost treatments had the resulted in the largest increment in soil respiration on both soil types.
• The soil biomass N content was significantly increased in both soils by the compost treatment, while in the case of the humus sandy soil, the Bactofil treatment also resulted in a significant increment.
• Urease enzyme activity was significantly increased by the artificial fertilizer treatment on both soils. In calcareous chernozem soil, the Bactofil treatment resulted in a slight (not significant) reduction in enzyme activity. In humus sandy soil, the Bactoful treatment also resulted in a slight reduction, while the compost treatment increased (though not significantly) the urease activity.
Based on our results, it can be stated that all three treatments were effective with respect to the studied soil microbial parameters. For both the calcareous chernozem and the humus sandy soil, the organic fertilizer Bactofil and the compost with high organic matter content had a stronger effect on some soil microbial parameters than the artificial fertilizer. -
Impact of ammonium nitrate and Microbion UNC bacterial fertilizer on dry matter accumulation of ryegrass (Lolium perenne L.)
35-39Views:80Pot experiment was performed to investigate the effects of increasing NH4NO3 doses with or without Microbion UNC bacterial fertilizer
application on dry matter production of ryegrass (Lolium perenne L.). Experiment was set up on calcareous chernozem soil of Debrecen-Látókép and on humus sandy soil of Őrbottyán. The bi-factorial trials were arranged in a randomized complete block design with four replications. Grass was cut three times. Dry matter production was determined and the sum of biomass of cuts was calculated as cumulated dry weights. Analysis of variance was carried out on the data in order to provide a statistical comparison between the treatment means. The least significant difference (LSD5%) test was used to detect differences between means. On the basis of our results it can be concluded, that the dry weights of ryegrass cultivated on chernozem soil were higher than on sandy soil. With increasing nitrogen supply the dry matter production of grass significantly increased in both types of soils. In case of sandy soil the increasing effect was more expressed, but dry weights of this soil never reached the appropriate values of chernozem soil. Application of Microbion UNC had positive effect on dry matter production of ryegrass grown on both two types of soils but the effect was more expressed on chernozem soil. Finally it can be concluded that the increasing effect of NH4NO3 on biomass weights was more expressed in both types of soils, the biofertilizer application also increased the dry weights of plant in a small degree. -
Az NPK-trágyázás hatása a kukorica tápelemfelvételének dinamikájára, öntözött és nem öntözött viszonyok között
23-27Views:155The effect of NPK-fertilization on the dynamics of nutrient uptake of maize (Zea mays L., cv. Clarica) was examined on chernozem soil under irrigated and non-irrigated conditions in a field experiment.
The following results were made:
• the element concentrations in the plant decreased over time,
• there is no difference between the dynamics of nutrients on irrigated and non-irrigated sites because rainfall was satisfactory for plants in vegetation period,
• the N doses not only significantly increase the nitrogen content in maize, but also have a noticable effect on Ca and Mg concentrations,
• because of the acidifying effect of N-fertilizers, increasing the amount of N-fertilizer increased the Mn, Zn, Cu content of the plants,
• the P doses have a significant effect on the maize P and N content and the Zn concentration of the plant via P-Zn antagonism in the soil,
• as the high K doses treatments alter the ion ratios in the soil, the Ca, Mg content of the plant decreased. -
The Effect of Sowing Time and Plant Density on the Yield of MaizeHybrids
95-104Views:71The crop technology of maize has two important elements, sowing time and plant density. In 2003 and 2004 we studied the effect of these two factors on the growth and production of maize in an experiment carried out near Hajdúböszörmény.
The soil of the experimental plots was meadow soil.
Weather in both years was differed greatly. 2003 was drought. Neither the distribution nor the quantity of the precipitation were suitable in the growing season for maize. This fact basically determined the results.
In 2004, we could talk about a favorable and rainy season. The distribution and quantity of precipitation was suitable between April and September. The average temperature was also suitable for maize.
Results of the sowing time experiment:
In 2003, we tested seven hybrids at four sowing times. Hybrids in the early maturity group gave the highest yield at the later sowing time, while the hybrids of the long maturity group gave it at the earlier planting time. The yield of PR34B97, PR36N70, PR36M53 hybrids was the best at every planting time. The moisture loss of hybrids in the late maturity group was faster in the maturity season, but the seed moisture content was higher than the hybrids with early sowing time. The seed moisture content was very low due to the droughty year. In two hybrid cases, this value was higher than 20% only at the fourth sowing time.
In 2004, we examined the yield and seed moisture content of nine hybrids. In the favorable crop year, the yield of every hybrid was the highest at the second and third sowing time. Yields of PR34H31 and PR38B85 hybrids were significant. The seed moisture content at harvest was higher than the previous year due to the rainy season. In the case of hybrids sown later, this value was higher by 30%. However, we noticed that this value was lower at the earlier sowing time than at the later.
The crop year had a more dynamic effect on maize than the sowing time. First of all, the quantity and distribution of precipitation played an important role in respect to yield safety.
Results of the plant density experiment:
We tested the reaction of hybrids at four plant densities (45,000, 60,000, 75,000 and 90,000 stock/ha) every two years. In 2003, the tested seven hybrids reached the highest yield at the 90,000 stock/ha in the face of a droughty year. The effect of forecrop and favorable nutrients caused these results. In the rainy 2004 year, the yield grew linear with the growing plant density. The yield of the best hybrids were 14-15 t/ha at the 90,000 stock/ha.
Such a high plant density (90,000 stock/ha) couldn’t adaptable in farm conditions in rainy season. It is practical to determine the interval of plant density besides the optimum plant density of hybrids which gave correct yield. The farmers have to use the low value of this interval due to the frequent of the droughty years. -
The Carpathian lingonberry, raspberry and blackberry fruit extracts feature variable antimicrobial efficiency
27-32Views:306Wild berry is an excellent source of phytonutrients and/or bioactive compounds associated with significant therapeutic properties, so that they have been utilized in folk medicine and traditional nutrition throughout centuries. Multiple health-promoting effects, such as anti-inflammatory, anti-diabetic, anti-heart and coronary disease properties were attributed to such wild berries. It has also been proved that berries could feature antimicrobial effects that could be of a great importance for the prevention of food-feed poisoning and fighting back antibiotic resistance.
In this study, we investigated the antimicrobial properties of lingonberry (Vaccinium vitis-idaea), raspberry (Rubus idaeus) and blackberry (Rubus fruticosus) crude and ethanolic extracts prepared from fruits obtained from the spontaneous flora of Eastern Carpathian Mountains situated in Transylvania. The antimicrobial effect of crude and alcoholic extracts were assessed on four Gram-negative, five Gram-positive bacteria and one yeast species using the agar diffusion method. The studied bacteria can cause food or feed spoilage and foodborne diseases. Our results indicate the significant inhibitory effect of lingonberry extracts in the case of Gram-negative bacteria like Proteus vulgaris and Salmonella Hartford, while among Gram-positive bacteria the strongest inhibitory effect was observed for Bacillus species like B. cereus, B. subtilis, B. mojavensis and Micrococcus luteus. The raspberry and blackberry extracts featured milder inhibitory effects in the case of the studied bacteria species. Furthermore, we have studied the crude or ethanolic extract combinations associated antimicrobial effects synergistic/additive or antagonistic properties. Interestingly, the triple and double ethanolic extract mixes had stronger antimicrobial properties, whereas the crude extract mixes showed relatively reduced effects, if any. Our results indicate that the antimicrobial activity of studied fruit extracts obtained from wild berries can vary upon the applied extraction method and their combination formulae, so that all these considerations must be taken into account when such fruit extracts are considered for foodstuff development.
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The effect of different genotype cattle grazing on the nutrient content of saline grasslands vegetation
46-50Views:173Maintaining saline grasslands in good condition, preserving their yield and diversity is important not only for the purpose of nature conservation, but also for farming. Therefore, the primary purpose of our study is to analyse the effect of pasture use of the smaller weight extensive and the larger weight intensive beef cattle on the grassland vegetation and nutrient content. In this way, we can answer the question whether grazing for nature conservation can be achieved with more profitable, more economical and more intense varieties.
The tests were carried out in May 2016 and May 2017, in the Hortobágy National Park (Pap-ere and Zám puszta), where a total of 16 sample areas were analysed. These areas are grazed with extensive beef cattle (Hungarian grey) and mixed genotype of intensive cattle. The associations were selected along a moisture gradient, such as wet salt marsh (Bolboschoenetum maritimi) and drier saline meadow (Beckmannion eruciformis). All the vegetative material collected both years in May was analysed for the following parameters: dry matter, crude protein, crude fibre and life-sustaining net energy content. We compared the effects of medium grazing (0.46 livestock/ha) and abandonment on vegetation and nutrient content.
We examined the effect of (i) grazing, (ii) different grasslands (salt marsh, saline meadow) and (iii) grazing of different cattle breeds (Hungarian grey, intensive beef) on the nutrient content of the vegetation of grasslands Based on our results, it was found that grazing had an impact on crude protein and life-sustaining net energy content. The highest crude protein content (12.75 m/m%) was obtained in the year 2017 in the area where higher density had been grazed for two years. For the lifesustaining net energy, the highest value (5.05 MJ/kg d.m.) was also obtained in 2017 and the lowest in 2016. Furthermore, it was found that there was no significant difference between the effect of the two cattle breeds on the parameters examined. Significant effects were observed only in the case of life-sustaining net energy: in the area of intensive beef cattle we received a higher value (5.15 MJ/kg body weight) than in the area with extensive beef cattle (4.96 MJ/kg body weight).
Our results have also shown that cattle grazing is of the utmost importance for the maintenance of both wet and mesophilous habitats. Based on our three-year study, we can say that grazing by both extensive and intensive cattle breeds is suitable for the management of saline habitats.
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Examination the effects of different herbicides on the soil microorganisms of a calcareous chernozem
121-126Views:74Pesticides play a key role in fighting weeds, pests and parasitic fungi. According to surveys, pests reduce the yield of agricultural crops by 35% worldwide. Pests, fungi and weeds account for 14%, 12% and 9% yield loss, respectively (Gáborjányi et al., 1995). Chemicals have contributed to increasing and maintaining the yields of crop production for decades. Today, agricultural production (in spite of many efforts) is unthinkable without the use of pesticides (herbicides, insecticides and fungicides). On the other hand, these chemicals contribute to the pollution of the atmosphere, surface and underground waters, and agricultural soils, especially if they are applied improperly.
The sustainable agricultural production pays attention to environment-friendly cultivation-technologies; but at the same time it makes an effort to produce good quality and economical products. The examination of the herbicides’ secondary effects, fits into this chain of idas namely, how the herbicides affect – stimulating or inhibiting – the soil microbiological processes, prevention of soil fertility.
In the course of the experimental work the effect of herbicides on soil biological properties were examined in different maize (Zea mays) cultures. We wanted wished to know that how the herbicides affect the quantity change of soil microorganisms, the life of different physiological groups of bacteria and the activity of microorganisms. A small pot experiment was set up in 2008 with the application of two herbicides - Acenit A 880 EC and Merlin 480 SC – in the breeding house of the Department. The moisture content and nutrient supply were at optimal level in the experiment.
On the basis of results the following can be stated:
1. It can be stated that the two herbicides and all their doses affected negatively the number of total soil bacteria, the
inhibiting effects were significant. The quantity of microscopical fungi increased by the effect of Merlin 480 SC and decreased in the treatments of Acenit A 880 EC.
2. The Acenit A 880 EC had stimulating effect on the nitrate mobilization. The CO2-production was stimulated by the basic doses of herbicides; the other treatments did not influence the CO2-production significantly.
3. The quantity of microbial biomass-carbon –except for only one treatment- decreased significantly by the effect of herbicides. Besides it, the quantity of microbial biomass-nitrogen increased significantly in the treatments of Acenit A 880 EC.
4. The biomass of test plant decreased in the treatments of herbicides, their quantities were smaller than in the control. In the pots treated by Merlin 480 SC, parallel with the increase of doses decreased the quantity of plant-biomass. -
The Effect of Forecrop and Plant Protection on the Pathology Parameters and Yields of Winter Wheat
84-89Views:88We carried out our experiment in the cropyears of 2000/2001, 2001/2002 and 2002/2003, on calcareous chernozem soil, at the experimental site of the Debrecen University Farm and Regional Research Institute, at Látókép. We examined the disease resistance and the yield quantity of Mv Magvas variety by adopting different forecrops and plant protection technologies, at 30+30 N level and at normal cereal row spacing. We applied two forecrops (wheat and pea) and two plant protection technologies (extensive and intensive). We measured the rate of infection by population survey in the first ten days of June.
In the course of our examinations, we found, that the rate of powdery mildew infection was higher in the thicker population sown after pea forecrop in all three years, as powdery mildew is not a typical cereal disease.
The infection rate of leaf mildew and DTR (Dreschlera tritici-repentis) was higher after wheat forecrop in all examined years, because these are typical wheat diseases and infection centres in the soil promote the spreading of these diseases. However, it was possible to parry the adverse effect of forecrops by intensive plant protection.
Due to the chernozem soil, wich has good water management features, and due to the good preparation of the seedbed, the effect of forecrops on yield quantity did not appear in the examined years. The quantity of the yield was only slightly larger after pea forecrop in the cropyears of 2000/2001 and 2002/2003 than after wheat. Nonetheless, the data of technical literatures state that the yield quantity can be larger, even by 15-20%, after pea forecrop.
In the course of intensive plant protection technology, we applied systemic pesticides, while in the course of environmentally sound technology, we used contact pesticides of sulphur content. In those populations that were treated with environmentally sound plant protection technology, infection rate was higher in all three years.
Yield quantities were somewhat lower in the course of applying extensive, environmentally sound technology, because diseases appeared in these populations to the higher degree. Powdery mildew does not, but leaf mildew and Dreschlera tritici-repentis have a significant yield decreasing effect. With appropriate, well-selected fungicides, we were able to keep every leaf diseases well in hand, and the rate of infection was almost independent of the influence of the breeding year. -
Effects of different crop years and sowing date on maize yield
93-96Views:123We carried out the tests in the flood meadow soil formed on the alluvial cone of Nagykereki, Sebes-Körös belonging to the Bihar plane small region. The aim of the study was to analyse the effect of the different sowing date of maize on the yield trend based on a comprehensive study conducted for 6 years (2007–2012).
The sowing date of maize hybrids is a factor that significantly influences yield, however, its effect is not significant in each crop year. In the years when the date of sowing has a modifying effect, the reliable yield level can be reached with optimal sowing date management (24 April).
The advantage of early sowing (10 April) proved to be dominant in the year of 2012, the seeds were placed into the still wet soil therefore shooting was more balanced. Maize seeds sown at the time of optimal (24 April) and late (10 May) sowing dates were placed into the already dry soil, which deteriorated germination and the strength of early initial development that had an effect on the yield.
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Effect and interaction of crop management factors and crop year on the yield of maize (Zea mays L.)
31-41Views:186The aim of this study was to determine the combination of treatment levels of crop management factors which can optimize and sustain maize yield under varying climatic conditions. The effect of winter wheat forecrop, three tillage systems (Mouldboard-MT, Strip-ST, Ripper-RT), two planting densities (60,000 & 80,000 plants ha-1), three fertilizer levels (N0-control, N80, N160 kg ha-1) with four replications in irrigated and non-irrigated treatments were evaluated over a five year period, 2015–2019. The obtained results revealed that growing season rainfall positively correlated with yield, whereas, temperature negatively correlated with yield. Impact of adverse weather on yield was less severe in biculture, irrigated plots, at lower planting density (60,000), lower fertilizer rate (N80) and in RT and ST, compared to MT. In years with favorable rainfall, yields of MT and RT were significantly (P<0.05) higher than ST. However, in a less favorable year, such as 2015, with 299 mm growing season rainfall and the lowest July rainfall (59% below mean) there was no significant difference (P>0.05) in yield among the three tillage treatments. Higher planting density (80,000), and fertilization rate (N160) in tandem with MT are treatments combination conducive for high yield under favorable climatic conditions, whereas, in years with low rainfall and high temperatures, RT and ST offer alternative to MT for optimum yield with 60,000 plants ha-1 and N80 treatment level. Crop year effect accounted for 20.7% of yield variance, fertilization 35.8%, forecrop 12.8%, plant density 3.4%, tillage 1.2% and irrigation <1%. It is conclusive that with proper selection of the appropriate levels of agrotechnological inputs the adverse effect of weather on yield can be mitigated.
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Changing of some parameters of the soil-plant system as an effect of different composts
262-266Views:80Composting is an alternative way for practicing site-specific and environmental friendly plant nutrient supply. Our aim was to study the effect of different composts on plant and soil system.
Pot experiment with acidic sandy soil blended with three composts in five (0%, 5%, 10%, 25% and 50%) proportion was set up. Our experimental plant was perennial ryegrass (Lolium perenne L.). After the harvest of ryegrass we measured the fresh and dry weight of harvested plants, the P-, K-, Mg-contents, and the pH of soils.
The three composts had different effect on dry weight production and on nutrient-supply of soil. We established that one of the compost had significantly larger effect on the dry weight of ryegrass compared to the others. In this study we proved that favourable compost/soil proportion is different in the case of different composts. -
Studies on the development of food attractants catching noctuid mouths (Lepidoptera: Noctuidae)
86-91Views:150The monitoring of the most dangerous noctuid pests (Lepidoptera: Noctuidae) can be performed by species specific pheromone traps. Recently the development of traps catching female moths became the main objective of the studies. We studied the synergistic effect of vine and beer as natural additive on the effectiveness of baits containing isoamyl alcohol and acetic acid in Forró and Debrecen-Ondód. The addition of vine and beer had positive effect on catches and it was significant in case of two dangerous pest species Agrotis segetum and Lacanobia oleracea. The synergistic effect was also significant with regard to the number of detected species especially in case of lower abundances and rare species.
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‘Kindergarten’ keeping-system in farrowing house: effect the socialization of piglets on weight performances, fecal cortisol metabolite level and post-weaning behavior
167-174Views:148This study aimed to examine whether litters’ let-together system before weaning (‘kindergarten-system’) has any stress effect and effect on post-weaning behavior, respectively. One week before weaning piglets were socialized by removing four adjacent farrowing crate walls. After weaning, piglets from the same experimental groups were housed in the same rearing crates. The piglet’s body weight was measured at birth than weekly till the 7th life-week. Fecal cortisol metabolites were measured for evaluation of the adrenocortical activity. Personal observation and evaluation were carried out for behavioral analyses after weaning. There were no significant differences in weight development and cortisol metabolite levels between the control and experimental groups. Behavioral analysis showed that pigs grow up in a socialized system, rapidly evolve ranking in the rearing crates, and the self-maintenance and social behavior were more pronounced. In control groups, the activity involving movement (exploring, walking, and running) was much more decisive. Recent work suggested the beneficial effect of the ‘kindergarten’ system in the farrowing house in animal welfare aspects.
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The effect of NPK fertilization and the number of plants on the yield of maize hybrids with different genetic base in half-industrial experiment
103-108Views:178In our research we examined the effect of the hybrid, the nutrient supply, the number of plants and the abiotic factors (temperature, amount of precipitation) on the yield, crop quality and yield stability of maize. We devoted special attention to the natural nutrient utilization ability and fertilizer reaction of maize.
The experiment took place in Hajdúszoboszló on chernozem soil, on a nearly 8 ha field. The size of one plot was 206 m2, this it was a halfindustrial experiment. We tested six hybrids with different genetic characteristics and growing seasons. I analysed the correlation between the nutrient supply and the yield of maize hybrids with control treatment (treatment without fertilization) and with N 80, P2O5 60, K2O 70 kg ha-1 and N 160, P2O5 120, K2O 140 kg ha-1 fertilizer treatments. Yield increasing effect of the fertilizer also depended on the number of plants per hectare at a great extent. The number of plants of the six tested hybrids was 60, 70, and 80 thousand plants/ha.
In Hajdúszoboszló, in 2015 the amount of rainfall from January to October was 340.3 mm, which was less than the average of 30 years by 105.5 mm. This year was not only draughty but it was also extremely hot, as the average temperature was higher by 1.7 °C than the average of 30 years. In the critical months of the growing season the distribution of precipitation was unfavourable for maize: in June the amount of rainfall was less by 31mm and in July by 42 mm than the average of many years.
Unfavourable effects of the weather of year 2015 were reflected also by our experimental data. The yield of hybrids without fertilization changed between 5.28–7.13 t ha-1 depending on the number of plants.
It can be associated also with the unfavourable crop year that the yield of the six tested hybrids is 6.33 t ha-1 in the average of the stand density of 60, 70 and 80 thousand plants per hectare without fertilization, while it is 7.14 t ha-1 with N80+PK fertilizer treatment. That increase in the yield is only 0.81 t ha-1, but it is significant. Due to the especially draughty weather the yield increasing effect of fertilizers was moderate. In the average of the hybrids and the number of plants, increasing the N80+PK treatment to N160+PK, the yield did not increase but decreased, which is explicable by the water scarcity in the period of flowering, fertilization and grain filling.
The agroecological optimum of fertilization was N 80, P2O5 60 and K2O 70 kg ha-1. Due to the intense water scarcity, increased fertilization caused decrease in the yield. As for the number of plants, 70 000 plants ha-1 proved to be the optimum, and the further increase of the number of plants caused decrease in the yield.
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Effect of year and irrigation on yield and quantity of different potato varieties
7-14Views:78dramatically in the last few decades. We are lagging behind Western European countries as regards yields. The ecological conditions are not favourable for potato, but the low yields other causes: the use of non-suitable seed, the low level of irrigation and nutrient supply and out of date machinery. The competitiveness of production is further decreased by the great alternation in yields from year to year, unpredictable market conditions, poor consumption habit, and often the lack of quality products.
The experiment was carried out at the experimental site of the University of Debrecen, Farm and Regional Research Institute, at Látókép. In our experiment, we examined the yield and selected quality parameters of 9 medium-early varieties in large parcels. Of the examined varieties, 3 are of Dutch, and 6 are of Hungarian breeding.
The experiment was set up in 2004 and 2005, on 50 m2 parcels on calcareous chernozem soil after winter wheat (2004) and two rowed barley (2005) as a forecrop. The varieties were examined in 4 repetitions in randomized blocks, of which two repetitions were irrigated, and two were non-irrigated. We examined the yields of the varieties, the distribution of tubers according to size and their percentages and changes, and selected parameters of quality and chemical composition due to irrigation. We studied the dry matter content, the starch content, the underwater mass, the amount of reducing sugars, and the colour index of frying of the tubers.
Summary, it can be stated that among the agrotechniques, year effect, variety and irrigation factors have considerable impact on potato yield quality and quantity. However, the impact of irrigation depends on the year effect. -
Mitigation of the effect of secondary salinization by micro soil conditioning
115-119Views:182This research has the general goal to meet the customization of agriculture in small scale farming. We are developing a technique using micro doses of soil conditioners and organic material applied in the root zone of vegetable crops. We expected to change the physical and chemical properties of the affected soil, which has been irrigated with salty water. Two different soil conditioners were tested. A lysimeter experiment including 8 simple drainage lysimeters was set up in the Research Institute of Karcag IAREF University of Debrecen in 2017. The main goal was to study the effect of different soil conditioners on the soil endangered by secondary salinization induced by irrigation with saline water. In order to compare the difference between the treatments, we collected soil samples, water samples, and determined the yields. Chili pepper (Capsicum annuum) was used as an indicator crop during one specific agricultural season. The technique called micro soil conditioning is rational because several reasons. The roles of the technique are various, for example it can serve as a source of carbon or a container for soil amendments and can minimize evaporation. We found this technique not to interfere with the chemical reaction or the interaction with the plants. However, the micro doses of soil amendments had the role to minimize the risk of soil degradation and do not significantly influence soil respiration. In addition, by improving soil properties, soil conditioning increases the leaching of the excess of salts from the root zone. In fact, this technique can decrease the cost of the inputs and improves the production of vegetables, and at the same time mitigates the effect of secondary salinization.