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Identification of environmental costs for agricultural companies – methodological questions and problems
177-183Views:78It is the consensus in the economic literature that corporate leaders do not know accurately the magnitude of environmental costs in their firms, and this lack of information is often a barrier to making environmentally-friendly decisions. For this reason, an important task of environmental accounting is identification of these costs, and a more realistic distribution of them among the products.
In this paper, we carried out identification of costs and expenditures for Hungarian agricultural companies.
We have dealt with this theme previously, but without detailing the related methodological questions. Detailed discussion is necessary because of the novelty of the subject (environmental accounting for agricultural firms).
Therefore, we elaborate the following questions: scope of determination of environmental costs and expenditures; methods for quantifying specific items; technique for their accounting. We also attempt to confront different viewpoints in the literature. Differences in accounting found between companies investigated are also presented.
The importance of our investigation is related to the significant increase in the number of environmental statutes that concern agricultural companies (e.g. rules for waste treatment, environmental taxation, introduction of charges and fees, regulation on liquid manure). Following these regulations puts an increasingly significant burden on the companies; therefore, the quantifications of environmental cost items has a growing
importance. -
White rust species (Chromista, Peronosporomycetes, Albuginales, Albuginaceae) on common weeds in Hungary
30-33Views:204The obligate plant parasite fungi in the family Albuginaceae are responsible for causing white rust diseases on weeds and they are rather common worldwide. Weedy plants with characteristic symptoms have been collected in 2014 and 2015 on location Hajdú-Bihar and Jász-Nagykun-Szolnok counties in Hungary. The determination of the species were based on the morphological characters both pathogens and hosts. Albugo candida was determined on shepherd’s purse (Capsella bursa-pastoris). Common purslane (Portulaca oleracea) is a host for Wilsoniana portulacae. The fungus Wilsonia bliti (syn.: Albugo bliti), the causal agent of white rust disease was found on redroot pigweed (Amaranthus retroflexus).
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Possibilities of mass valuation in land use in Hungary
59-68Views:154Technological development makes it possible to simplify and accelerate decision-making processes by adequately processing and evaluating large volumes of data. Sub-data obtained from large data sets have a very important practical role in asset valuation, forecasting and valuing delineated or difficult-to-map areas, or in the context of portfolio management. Land valuation is a separate segment within asset valuation and it requires a specific methodological approach on behalf of evaluators. In this study, the authors compared the transaction data of arable land and the value of other land use categories. Based on empirical assessments, the authors developed proposals for the fast and cost-effective determination of the value of land use categories other than arable land - mainly meadows and pastures.
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New possibilities to determine wheat quality parameters by near-infrared spectroscopy
65-69Views:139Analyses and methods in wheat quality determination require more sample, time, work and cost, thatswhy flour quality control needs rapid, reliable tools. Near-infrared spectroscopy has many advantages, which make it suitable in quality control. NIR instruments need calibrations to their work. In our study we examined gluten content, falling number, valorigrpahic waterabsorption, alveographic P/L and W value of wheat samples. Modified partial least squares analyses on NIR spectra were developed for each property. The results show that we got such calibration modells, which are able to predict the properties (expect falling number) with enough accuracy.
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Effects of Cropping Year and Artificial Fertilization on Alveographic Quality of GK Öthalom Winter Wheat Variety
126-133Views:89Qualification of winter wheat became more complex for Hungary, after the expansion of the EU. Use of Chopin alveograph is a general method in Western- and Southern Europe. This method is not traditional in Hungary, so determination of alveographic properties of Hungarian winter wheat varieties is very important. Artificial fertilization is one very important component of applied agricultural engineering, which effects winter wheat quality. The another factor is the weather or rather the cropping year, which effects winter wheat quality too. We examined both of them and the alveographic quality of GK Öthalom winter wheat variety between 1996 and 2003.
We found that cropping year has significant effect on the alveographic quality of GK Öthalom winter wheat variety on each treatment of artificial fertilization. We found no connection between quantities of precipitations of vegetation periods and the W values of GK Öthalom.
We found a strong connection in 1999 and 2003 as well as a very strong connection in 1996, 1997 and 2002, between the increase of fertilizer dose and W values of GK Öthalom. Treatments of artificial fertilization had a significant effect to W values of GK Öthalom in 2001 and 2002. The differences between the two methods are because of large standard deviation of the results. -
Evaluation of various silo sorghum hybrids from the energetic aspect
107-111Views:121The sweet sorghum is a perspective plant of bioenergy, which can be the foundation of Hungarian bioethanol production in the future. By the examination six sweet sorghum hybrids have been examinated by the viewpoint of sugar aggregation and bioethanol production capacity. The founda tion of the surve y was three growing se ason (201 0, 2011, 2012 ). The ex per ime nt w as on the production sites of U niversity of Debrecen CAAES RISF Karcag Research Institute. The production site of the sweet sorghum have been sampled by samples of 1 m2 by hybrids within the period of august 15 and november 15. The sugar content of the samples have been measured by refractometer which was the base in the determination of ethanol production capacity.
As a continuation of the evaluation of energetic viewpoint the Higher Heating Value (HHV) have been measured from the bagasse of sweet sorghums. According to the results it can be stated that int he case of sugarcontent and the dinamics of the sugar aggregation can show several differences, while HHV of sorghum bagasse is within 16 200 and 16 900 J g-1.
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Environmental Consequences of Efficient Use of Nitrogen Fertilizers
41-46Views:73Nitrogen fertilizer represents major economic burden. For this reason, although the efficiency of nitrogen utilization varies highly, its actual use generally remains at low levels; these averaging between 25 and 50%. We set up an experiment at the Oradea Research Station, using 15N labeled fertilizers, in order to investigate the possibility of increasing N fertilizer efficiency in winter wheat under irrigation conditions.
Fertilizers labeled with 15N allows us to individually determine its effect on yield formation, as well as the use efficiency of N from fertilizer following application rate and time. The amount of N derived from fertilizer as determined in straw and grain yield is high. When the labeled fertilizer is applied at tillering time, the values of this indicator rise when higher N levels we applied.
In separate experiments, we investigated a series of aspects connected to chemical fertilizer regarding the determination of the type of fertilizer, optimum time and rates of application; all these as a function of the special pedoclimatic conditions.
The results obtained in the field show that the effectiveness of N utilization in wheat is most variable and generally low, often ranging between 25 and 33%, owing to N loss within the system through leaching and NH3 volatilization.
A readily achievable increase in efficiency of 5 percentage points would result in considerable savings, and can be brought about by reducing nitrogen losses. The added benefits to the environment in terms of reduced ground/water contamination and lowered nitrous oxide (N20) emissions would also be substantial.
The figures for N fertilizer use efficiency (% N range from 35.5 to 72.6, the highest value being recorded with an N application of 120 kg/ha at tillering, when the previous crop was sunflower).
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The effect of dried herbs on the souring of sunflower oil
87-89Views:120Many herbs have antioxidant activity, which can replace the use of potencionally harmful synthetic antioxidants. Sunflower oil fortified with eight different plants and stored at room temperatur in my experiment. Specified intervals with determination of peroxide value, FFA, iodine value, anisidine value and TOTOX value followed the quality of the sunflower oil.
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Assay of runoff conditions using a Digital Elevation Model
124-129Views:91We can get information about water conditions of plane areas by analyzing their relief. By using the Digital Elevation Model, we can get proper information about runoff conditions in the terrain surface, which is the basis for ponding analysis. In our study, a Digital Elevation Model of the sample plot in Hajdúsági-löszhát (loess ridge) was created that makes possible the determination of runoff conditions of the surface. Convergence and divergence of runoff direction showed the rate of ponding and the location of possible inland-water spots. By using this model, concrete location of those areas that tend to be overmoistured could be determined. Knowledge of this phenomenon can be an effective instrument for farmers in optimizing crop growing, and additionally in performing water management interventions in proper time and space.
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New wonder of an old hormone: Hungarian sour cherry varieties as natural melatonin sources
65-72Views:174Agriculture has always been an important role in economy, food supplies, sustainability of society During our experimental work an effective extraction procedure has been developed for melatonin. Further chromatography was developed the quantitative and qualitative determination of melatonin. A compound that was equal to the standard was purified from the extract by preparative HPLC technique and the structure of the purified sample was confirmed by MALDI-TOF-MS and NMR analysis. Based on our measurements, harvested in the state of biological maturity Hungarian sour cherry cultivars contain high levels of melatonin.
Our results show that "VN4" variety has extremely high melatonin content, 9.893 ug g-1 and suggest that "VN4" which were selected from the "Bosnyák" varieties is melatonin accumulating. The average value of the melatonin content of Hungarian sour cherry cultivars is 2.319 ug g-1.
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Coagulase-negative staphylococci in ewe udder surface and raw milk samples
221-225Views:132Coagulase-negative staphylococci (CNS) are among the major causes of subclinical mastitis in dairy ewe flocks. This has a financially significant impact on the ewe dairy sector and consumer health. The determination of the bacterial count, particularly CNS, is critical in terms of the quantity and quality of ovine milk. Thus, the purpose of this study was to quantify the CNS count in the udder surface and raw milk samples of the ewe, in addition to identifying CNS strains (n=8) collected from udder surface and individual raw milk samples by 16S rRNA gene sequencing. A total of 164 samples of udder surface and raw milk originated from four sheep farms were tested. The obtained values were compared between the different farms. Furthermore, values during 2018 and 2019 in the case of Farm I were compared. Significantly higher (p<0.05) average count was observed in udder surface samples taken from Farm I (2.8±1.0 lg CFU/cm2) than that of Farm III samples (2.3±0.6 lg CFU/cm2). In the case of individual raw milk, the higher value was observed in samples derived from Farm III (3.5±0.9 lg CFU/mL), while the obtained value was significantly lower (p<0.05) in samples originated from Farm IV (1.8±0.4 lg CFU/mL). In the bulk tank milk samples, the highest mean value was 5.3±0.4 CFU/mL, and there was no significant (p>0.05) variation between farms. Coagulase-negative staphylococci counts were decreased to a certain extent in both sample types tested during 2019 except for individual raw milk derived from the Tsigai breed. The correlation between the mean CNS counts of udder surface and individual raw milk was very weak (r=0.048). Staphylococcus simulans, Staphylococcus auricularis, and Staphylococcus equorum were identified by molecular sequencing and Staphylococcus simulans were the most frequently identified CNS species. A higher CNS count of bulk tank milk than individual raw milk indicates possible contamination during milking and storage. Therefore, further studies are required to investigate the other sources of bulk tank milk contamination to improve the hygienic quality of milk.
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Methodological development of the determination of the compost mixture ratio
29-33Views:72The mixing rate of the compost raw materials basically determines the procedure of degradation. In most cases, in practice the real mixing ratio is not equal with the counted ratio – which based on the C/N ratio – and the check of the fulfilled mixing is not possible.
The homogeneity of the prism is also one of the fundamentals of the effective degradation process. There is no available method to determine the homogeneity of the compost prism.
During our research we examined the reflectance values of different compost mixtures where sewage sludge and rape-straw were used as raw materials.
According to our results we built up a regression model which gives the ability to determine the mixing rate (in 5% margin of error) and makes possible to check the homogeneity of the prism together with the access of immediate action. -
Application of the Collision Cell (CCT) in the ICP-MS Analytical System
120-125Views:130Our laboratory has seen a sharp rise in the number of requests for the analysis of smaller and smaller concentrations of elements from foods, plants, soil, organic fertiliser, irrigation and ground water, sewage, sewage sludge, raw material of food, as well as human and animal origin samples. From the above elements the inductively coupled plasma mass spectrometer is capable of analysing the smallest concentration of elements. Our method of analysis is to use our ICP-MS instrument, together with a ICP-MS with Collision Cell Technology (CCT). The CCT method has better detection limit, with 1-3 magnitudes, compared to the normal ICP-MS analytical method. The CCT has better detection limits mainly for the following elements in the periodical system: analysis of arsenic, selenium, germanium, vanadium and chromium. Additionally a collision cell can be applied for the analysis of silicium, sulphur, zink, copper, iron, calcium, magnesium and potassium in smaller concentrations.
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Interpretation of rurality and the situation of land use in Hungary
79-85Views:160The summarizing data collection of our study has been carried out in the scope of the FP7-REGPOT-2010-1 ’UD_AGR_REPO’ project as a part of the cooperation with the University of Lincoln. The University of Lincoln is an important partner of the project, the knowledge transfer activities that have been carried jointly with them are multilateral. One of the most important cooperation areas is the analysis of rural areas, rurality itself, determination of breakout points, exploration of alternative income sources, diversification possibilities. Some part of the work of the University of Lincoln on the field of rural development is based on the assessment and documentation global similarities and differences of rural areas. Present study also contributes to that work, it has been prepared on the request of the University of Lincoln with the aim of providing insight into the special political and economic changes/processes that took place in Hungary, and through them into the structure and operation of the unique Hungarian rural areas.
The study first positions the definition of rurality and rural areas into context on the basis of official EU and Hungarian legal classification. Then it covers the important agricultural nature of Hungary, which significantly determines the possibilities and properties of Hungarian rural areas. The further description of rural areas is completed by some historical summary, the introduction of ownership changes, detailed description of employment and income conditions and finally by the listing of breakout points of rural areas as a conclusion.
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The possibility of use of the 0,01 M CaCl2 and Baker- Amacher extractants for the determination of plantavailable potassium
7-15Views:71The Hungarian fertilizing recommendation systems use AL soil test for the evaluation of potassium supply. The 0.01 M CaCl2 is a definitely milder extractant, it extracts the easily soluble and exchangeable potassium amount. Its European introduction was already taken into consideration in 1994. The research project on this topic is started in several european countries, also in Hungary at the Department of Agricultural Chemisty of Agricultural University of Debrecen. Another advantage this multielement method is that the different element-ratios can also be calculated.
The Baker-Amacher extractant’s principle is that it contains a known amount of K, P, Mg in the CaCl2 solution. During the soil extraction adsorption and desorption process take place, so the adsorption or desorption can be calculated from the original and the final concentrations.
In this paper we introduce the results of comparing analysis of the samples (n=630) from Soil Information and Monitoring System. Our aim was to measure the use of new extractants beside conventional extractant (AL) for the evaluation of K-supply would be reasonable.
It can be stated that there is a medium close relationship (r=0.75) between AL-K and 0.01 M CaCl2-K. My calculations confirmed the results of former examinations, and proved that the two extractants don’t extract and change the same rate of K-fractions. We found that regression between 0.01 M CaCl2 and AL depend on texture classes, pH classes, amount of lime, and organic matter content of soils.
Comparing the relations between AL and Baker-Amacher we find relatively loose correlation (r=0.45). We stated that there are K-fixing soils among soils considered to be well supplied with potassium by AL. This might be caused by the high amount of mineral clay and the quality of mineral clay. We stated that the dK averages show that the Hungarian nutrient-supply categories characterize generally well K-supplement of soil.
It can be stated that it would be necessary to use new extractants to specify evaluation of plant available K. We found that the 0.01 M CaCl2 and Baker-Amacher extractants could complete usefully the AL procedure and could help effective potassium fertilization. -
Changes in the Diaporthe helianthi Infection of Sunflower Hybrids Between 1998 and 2002
214-233Views:65Plant protection, and especially the efficiency of protection against mycosis, is a very important production technological element concerning sunflower. The efficiency of production can be increased on the basis of a thorough pathological survey and its results carried out in a wide variety of hybrids, as the features of hybrids, the reactions of genetic bases to pathogens can be found out under domestic conditions.
The tests were carried out at variety-test lots of OMMI for hybrids used for food or oil and other sunflower varieties admitted by the state at the Experimental Site DTTI Látókép, Centre of Agricultural Sciences, University of Debrecen between 1998 and 2002. The number of the tested hybrids was 49 in 1998, 45 in 1999, 49 in 2000, 55 in 2001, and 44 in 2002.
Due to the infection source of high amounts remained from 1997 and the favourable weather conditions for the pathogens, an infection of high degree was experienced in the tested stands. The lowest infection index in the hybrids with a very short vegetative period was experienced with Beni hybrids. Comparing to the average of the hybridgroup with short vegetative period, a low infection index characterised the Baleno, Trident, IBH-166, Hysun 321, Resia, Alexandra, Cergold and Pixel hybrids. In 1999, among hybrids with medium vegetative period, Zoltán, Zsuzsa and Util hybrids could be highlighted because of their low infection index. In 1998, among confentionary hybrids, Marica-2 hybrid had the most favourable index values (2,55).
During our trials, the experienced infection dynamics were compared in the event of hybrids with higher and lower susceptibility under different sowing technological elements. The results call for the fact that when a hybrid with higher susceptibility is produced, production technological elements, such as the time of sowing, influence considerably the damage caused by the pathogen, therefore it must not be ignored when its determination takes place. In the event of hybrids with lower susceptibility the change of the tested production technological elements within the biological optimum does not lead to the increased risk of the damage caused by Diaporthe helianthi. -
Comparison of the sample preparation methods worked out for the examination of the element content of wine
77-82Views:113The examination of the potentially toxic elements content of the wines is not easy task, because the most elements are in little concentration (mg kg-1 or μg kg-1) in the wine and the wines contain great amount of organic matrix. The efficient sample preparation is essential for the accurate determination of element content. The eim of our research was to determine which sample preparation method will be the most efficient in examination of wines with ICP technology. The examined wine sample was a 2008 Chardonnay from the Eger wine region. We did the sample preparation and analysis examination in University of Debrecen, Centre for Agricultural and Applied Economic Sciences, Institute of Food Science, Quality Assurance and Microbiology.
We did the analysis examinations with ICP- MS (inductively coupled plasma mass spectroscopy). We always did the sample preparations and the examinations in three times rehearsal. The applied sample preparation methods: dilution with distilled water, open digestion and microwave digestion.
We were able to measure B, Al, Mn, Fe and Zn with only dilution and open sample preparation. In the smaller quantity present Sr and Ba were measurable in the wine in the case of all three methods well. We were able to measure the Co with dilution and open digestion method, while Cr, Ni, and Te with only dilution method. In the case of arsenic we were not able to measure reliable result with dilution and open digestion method because of organic matrix and other components
(alcohols, monosaccharides, polysaccharides, polyalcohols and inorganic salts). On the whole we are able to say that in the case of certain elements (B, Mn, Fe, Zn, Sr, Ba) the open digestion and dilution sample preparation is applicable well, however, in the case of certain elements (As, Al, V, Cr, Se, Mo, Cd, Hg, Pb) we have to develop the methods. It may be development of one of the way, if we develop sample preparation methods to examined element specifically and not
to wine generally. -
The effect of plant density on maize yield in average and extremely dry years
7-16Views:85The yield safety of maize has not been satisfactory in Hungary for decades. Yield is influenced by the combination of several factors.
In recent years, the frequency of dry years increased and fertilization decreased. These factors call for a rational determination of the plant density.
I studied the relationship between plant density and yield in 2003-2004 and 2007 on meadow soil.
In 2003, the weather was dry. In the vegetation period, the amount of precipitation was 78.5 mm lower and the temperature was 0.97 °C higher than the average of 30 years, the number of hot days was 47-60 (days with a temperature higher than 30 °C). However, we obtained favourable results under experimental conditions in 2003 after wheat as a forecrop using the fertilizer Kemira Power.
The weather in 2004 was favourable. In the vegetation period, the amount of precipitation was 93.2 mm higher than the average of 30 years. Although, the distribution of the precipitation could have been more favourable. The yield of the hybrids ranged between 8.87-10.42 t/ha. Among the studied seven hybrids, the early hybrids gave the highest yield at the highest plant density of 90 thousand plants/ha (PR38Y09, PR38A67, PR37D25, PR37M34). However, FAO 400-500 hybrids gave favourable results also at the low plant density of 45 thousand plants/ha (8-9 t/ha). At this plant density, the aeration of the plant stock was better and the hybrids were prone to bringing several cobs. Yield stagnated with increasing plant density (60 thousand plants/ha), then at 75-90 thousand plants per ha, the yield started to increase again.
In 2004 the yield of hybrids was considerably higher than in the previous year. In contrast to yields of 8.87-10.42 t/ha in 2003, yields in 2004 were around 9-12 t/ha.
The yield of the hybrid XO 902 P is above 12 t/ha already at a plant density of 45 thousand plants/ha. It gives maximum yield at the plant density of 90 thousand plants/ha.
The hybrid PR38P92 showed a good response to changing plant density, but its yield was only 9 t/ha at the low plant density value.
In a favourable year, the yield of the hybrids PR38B85, PR37W05, PR37D25, PR37K85 at a plant density of 45 thousand plants/ha 11 t/ha, while at the higher plant density of 90 thousand plants/ha, it ranges around 13-15 t/ha.Hybrids PR36K20, PR35Y54, PR34H31 have a good individual yield and they are prone to bringing several cobs in favourable years at a low plant density. Their maximum yield at the plant density of 90 thousand plants/ha is almost 16 t/ha.
In 2007, the weather was similar to that of the extremely dry year of 2003. The amount of precipitation in the vegetation period was 41.9 mm lower than the average of 30 years and its distribution was not favourable either.
In the optimum NPK fertilizer treatment at an optimum plant density, the yield of hybrids ranged between 9.32-10.73 t/ha. The highest yields of 10.22-10.73 t/ha were measured for hybrids PR38A79 (FAO 300) and PR35F73 at a relatively low plant density of 60 thousand plants/ha.
In the average of the hybrids, the optimum NPK dosage was N 131, P2O5 82, K2O 93 kg/ha active ingredient. -
Application of molecular genetic methods for birds (literature review)
37-41Views:119Ornithology studies have been extended by molecular genetic techniques. In this paper we are dealing with the most common use of areas. Our basic aim is to give a comprehensive view of the most commonly used methods in ornithological studies, including the available results by their use. We also deal with the following areas: an essential step during examination namely the applicability of sample collecting methods, and the unique identification, also the sex determination, methodology of DNA barcoding, as well as the role of molecular methods to protect endangered species. We discussed the advantages and disadvantages of the methods, such as the current trend for each method.
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Effect of net shading technology on the yield quality and quantity of chilli pepper under greenhouse cultivation
5-9Views:226This study was undertaken to identify the effect of net shading technology on the total capsaicinoids, vitamin C and relative chlorophyll content expressed as SPAD (Soil Plant Analysis Development) values and total marketable fruit yield of ‘Star Flame’ chilli pepper (Capsicum annuum) for two harvesting times cultivated under modified atmosphere. ‘Star Flame’ pepper was grown under three (3) different net shading colours (white, red and green). Samples without net shading were used as control. Samples were subjected to chromatographic analyses using HPLC for the determination of total capsaicinoids and vitamin C. Interaction in shading technology and harvest times (p=0.010) had significant effect on total capsaicinoids as a result of green and white shading technologies showing higher differences when compared to control samples. Vitamin C content was observed to have increased in white and red shadings (p=0.001) after the first harvest and gradually decreased after the second harvesting time (p=0.002). Relative leaf chlorophyll content was significantly higher in white shading in the first and second harvest. Samples used as control had significantly higher marketable values when compared to white and green shadings with red recording low marketability of ‘Star Flame’ chilli peppers.
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Determination of conversion rate values to characterise the ethanol production of the Kluyveromyces marxianus CBS712 fungus branch
23-27Views:70The ethanol production of the Kluyveromyces marxianus CBS712 strain was investigated among different kind of condition. We defined the conversion rate in order to know the efficiency of the ethanol production. To determine this value, it is crucial to characterize the residual glucose concentracio. We chose two method to determine the amount of residual glucose. The first method is the thin layer chromatography (TLC) and the second method is the method of the glucose-oxidase enzyme. We found that the TLC method is reliable than the other method. The conversion rates were determined from these values and the ethanol values. The maximal ethanol production of the characterized Kluyveromyces marxianus CBS712 strain (5.6% (v/v) ethanol at 45 °C, 55.3% conversion rate) is comparable to those strains which are applied in industrial ethanol production nowadays.
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Evaluation of the correlations between the normalized difference vegetation index (NDVI) and yield in a seasoning paprika (Capsicum annuum L. var. longum) stand
45-49Views:242The aim of our examination was to evaluate the correlations between the normalized difference vegetation index (NDVI) and yield, as well as to examine the possibility of yield estimation basedon NDVI in a seasoning paprika population.
Significant correlations were observed during the examination of the correlation between NDVI and yield. Furthermore, it was concluded that yield can be estimated with a 6.6–8.3% mean error based on the regression equations. No significant difference was shown between the error of estimations performed with various regression types and that of the estimations performed at various dates. For this reason, the identification of the optimum estimation method and the determination of the optimum date for estimation call for further examinations.
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Influence of phytophagous mammals environment-forming activity on the soil invertase fermentative activity in conditions of mining impact region
127-130Views:97Excretorial and fossorial activity of mammals is an important part of environment-forming activity. Mammals have influences on important biogeocenotic processes, especially on the soil processes. Determination the maintenance of soil invertase as one of diagnostic description the ecological state allowed defining limits of oscillation index in dump areas and in clean (control) native areas. The obtained results of the investigation indicate the soil depth, duration of experiment and type of area influence on soil invertase activity with the high statistical level of significance. Positive influence is revealed on invertase activity changing on dump areas, where an active excretorial and fossorial activity of phytophagous mammals was observed.
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SSR based characterization of peach (Prunus persica L.) and apricot (Prunus armeniaca L.) varieties cultivated in Hungary
17-24Views:263The SSR (Simple Sequence Repeat) markers allow the discrimination of the cultivars and determination its specific DNA fingerprints. The aim of this research was to evaluate fifteen apricot (Prunus armeniaca L.) and fifty-one peach (Prunus persica L.) genotypes cultivated in Hungary to obtain their DNA fingerprints in 6 SSR (Simple Sequence Repeats) loci by allele numbers and sizes.DNAs were extracted from leaves. PCR was carried out with CY-5 fluorescent labeled Prunus microsatellite markers and the products were separated on polyacrylamide gel with ALF (Automated Laser Flourometer)-Express II.According to our results, in the case of peach genotypes, all 6 SSRs were able to amplify alleles. UDP 96 005 was the most informative marker and UCDCH 17 was the least due to its monomorphic pattern. Regarding the apricot samples BPPCT 041 did not amplify any allele. In the case of P. armeniaca UDP 96 005 had the highest heterozygosity index as well and the highest number of alleles. The least informative marker was the UCDCH 17. Since the 6 SSR were not enough to discriminate the apricot and peach genotypes, it is suggested to use more SSR primers. -
Application of AFLP-Method in Plant Sample Identification
207-213Views:62One possible method for the determination of DNA-polymorphism is the PCR-based AFLP (Amplified Fragment Length Polymorphism). This method had been succesfully introduced to the Department of Botany at University of Debrecen in 2000-2001 with the examination of hay saffron (Crocus sativus L.) and its allies. Hay saffron is grown as a spice for some thousand years producing the most expensive spice in the world. This plant is sterile, triploid reproduces only vegetatively with no fertile seeds. However its origin is unknown it exists only in cultivation and it is a mutated variety of another species or an artificial or natural hybrid. Usual methods for the systematic examination are restricted hence it seemed to be reasonable to apply molecular biological methods in its case. Results of this work include the introduction and many fold application of the method beside ensuring the consequences of science literature with determining the C. cartwrightianus to give the most similar genetical pattern to C. sativus.