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New wonder of an old hormone: Hungarian sour cherry varieties as natural melatonin sources
65-72Views:174Agriculture has always been an important role in economy, food supplies, sustainability of society During our experimental work an effective extraction procedure has been developed for melatonin. Further chromatography was developed the quantitative and qualitative determination of melatonin. A compound that was equal to the standard was purified from the extract by preparative HPLC technique and the structure of the purified sample was confirmed by MALDI-TOF-MS and NMR analysis. Based on our measurements, harvested in the state of biological maturity Hungarian sour cherry cultivars contain high levels of melatonin.
Our results show that "VN4" variety has extremely high melatonin content, 9.893 ug g-1 and suggest that "VN4" which were selected from the "Bosnyák" varieties is melatonin accumulating. The average value of the melatonin content of Hungarian sour cherry cultivars is 2.319 ug g-1.
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Effect of fertilization on the potentially mineralize N forms of soil of long term field experiment was set in an acidic sandy soil
20-24Views:120The aim of this paper was to provide further information about the nitrogen mineralization processes of soil. A modified incubation technique was applied to establish the amount of easily soluble mineral and organic N forms during the incubation period. An acidic sandy soil was used for incubation, which was sampled from the „Westsik” long-term field experiment. The incubation was carried out on fifteen selected soil samples which were received different treatments since the experiment was set up.
From the obtained results, the amount of potentially mineralizable N and the mineralization rate constant were determined. Results of chemical analysis and biological interpretation of results are discussed. -
Susceptibility of stem infected sweet corn hybrids to common smut disease
55-57Views:368The common smut of maize (corn smut, Ustilago maydis /DC/ Corda) can cause large economic losses in susceptible sweet corn hybrids as well. The protection against this pathogen is fundamentally based on prevention. Many methods to control corn smut have been recommended or evaluated, including crop rotation, sanitation, seed treatments, modification of fertility, and biological control. In spite of these frequently mentioned control strategies, the host resistance seems to be the only effective method to manage common smut in those areas where Ustilago maydis is prevalent.
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Changes in the Diaporthe helianthi Infection of Sunflower Hybrids Between 1998 and 2002
214-233Views:65Plant protection, and especially the efficiency of protection against mycosis, is a very important production technological element concerning sunflower. The efficiency of production can be increased on the basis of a thorough pathological survey and its results carried out in a wide variety of hybrids, as the features of hybrids, the reactions of genetic bases to pathogens can be found out under domestic conditions.
The tests were carried out at variety-test lots of OMMI for hybrids used for food or oil and other sunflower varieties admitted by the state at the Experimental Site DTTI Látókép, Centre of Agricultural Sciences, University of Debrecen between 1998 and 2002. The number of the tested hybrids was 49 in 1998, 45 in 1999, 49 in 2000, 55 in 2001, and 44 in 2002.
Due to the infection source of high amounts remained from 1997 and the favourable weather conditions for the pathogens, an infection of high degree was experienced in the tested stands. The lowest infection index in the hybrids with a very short vegetative period was experienced with Beni hybrids. Comparing to the average of the hybridgroup with short vegetative period, a low infection index characterised the Baleno, Trident, IBH-166, Hysun 321, Resia, Alexandra, Cergold and Pixel hybrids. In 1999, among hybrids with medium vegetative period, Zoltán, Zsuzsa and Util hybrids could be highlighted because of their low infection index. In 1998, among confentionary hybrids, Marica-2 hybrid had the most favourable index values (2,55).
During our trials, the experienced infection dynamics were compared in the event of hybrids with higher and lower susceptibility under different sowing technological elements. The results call for the fact that when a hybrid with higher susceptibility is produced, production technological elements, such as the time of sowing, influence considerably the damage caused by the pathogen, therefore it must not be ignored when its determination takes place. In the event of hybrids with lower susceptibility the change of the tested production technological elements within the biological optimum does not lead to the increased risk of the damage caused by Diaporthe helianthi. -
Values of Hungarian pigeon breeding
9-14Views:153Pigeon breeding has a history dating back several centuries in Hungary. It has internationally renowned and accepted results. Our aim was to estimate the range of values related to Hungarian pigeon breeding and draw attention to the importance of their conservation. We studied the biological values of native breeds and the appearance of pigeon breeding within the Hungarian culture. These include the use of the symbol, the relating beliefs and customs, language, gastronomy, architecture and object values, as well as the community-building effect. Based on our results we can say that this topic is worthy of further research.
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Investigation of the effect of allithiamine-enriched feed on the poultry gut microbiome composition and resistome
149-155Views:66Over the past 20–25 years, the poultry industry has evolved into a specific protein production system. However, the stress resulting from intensive rearing practices has led to numerous negative consequences, making the optimisation of livestock gut microbiome composition crucial for mitigating these effects. Advancements in modern molecular biology methods have brought attention to the impacts of nutrients on gut microbiota. In our study, we extensively investigated the changes induced by feed formulations rich in phytonutrients on the gastrointestinal microbiota of livestock using targeted 16S rRNA amplicon sequencing. Our objective is to examine how the developed feed prototype affects the composition of core microbiomes in raised poultry, community diversity, and the resilience of complex microbial networks. We seek correlations between biological livestock and environmental samples to identify which community constituents, in what proportions and occurrences, may play a role in the development of specific diseases. Based on our measurement results, it can be asserted that allithiamine positively modulated "beneficial" community constituents. Beyond the impact of allithiamine-enriched feed rich in phytonutrients, the composition of the microbial community in the poultry gastrointestinal tract is significantly influenced by the age of the birds. Furthermore, due to the presence of multi-drug-resistant pathogens in environmental samples from livestock facilities, appropriate transmission risk management measures are of paramount importance.
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Environmentally-benign plant protection possibilities against domestic Monilinia spp. in organic apple and stone fruit orchards
101-105Views:115In this study, possibilities of environmental-friendly plant protection against domestical brwon rot species were summarized for oecological pome and stone fruit orchards. Symtomps of the two most important brown rot species (Monilinia fructigena (Aderh. & Ruhl.) Honey and Monilinia laxa (Aderh. & Ruhl.) Honey) were described and then cultivar susceptibility to brown rot was discussed. Furthermore, mechanical, agrotecnical, biological, and other control possibilities (stone powders, plant extracts and restricted chemical materials) were shown.
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Element content analyses in the Institute for Food Sciences, Quality Assurance and Microbiology
203-207Views:111The role of chemical elements to ensure and promote our health is undisputed. Some of them are essential for plants, animals and human, others can cause diseases. The major source of mineral constituents is food, drinking water has a minor contribution to it, so the knowledge of elemental intake through food is crucial and needs continuous monitoring and by this way it promotes the food quality assurance and dietetics.
With the evolution of spectroscopic methods increasingly lower concentrations could be determined, so the elemental composition of a sample could be more precisely and fully described. Due to the results the gathered knowledge up to the present is supported and new observations can be done helping us to understand such complex systems as biological organisms are.
The quality of a food is determined by the full process of its production, consequently it starts with agricultural production so elemental-analysis usually cover the whole soil – plant – (animal) – food chain, by this way the „Fork-to-Farm” precept is true in elemental analysis field also.
The history of elemental analysis in the University of Debrecen, Centre for Agricultural and Applied Economic Sciences, Faculty of Agricultural and Food Sciences and Environmental Management, Institute of Food Processing, Quality Assurance and Microbiology goes back to 1980s when the so called Regional Measurement Central gave the background for research. The continuous deployment resulted in an obtain of an inductively coupled plasma atomic emission spectrometer (ICP-AES) in 1988, which extended the scope of examinations due to its excellent performance characteristics
compared to flame atom absorption (FAAS) and flame emission spectrometers (FES). The instrumental park retain up to date correlate to the developing analytical techniques due to acquiring a newer ICPAES in 1998 and an inductively coupled plasma mass spectrometer in 2004 – which sensitivity is three order of magnitude better compared to ICP-AES. The Institute supports the work with its own ICP-AES and ICP-MS since 2011. -
Impact of fertilization on production capacity and reaction to fertilization in maize hybrids
42-46Views:66New varieties and fertilization have significantly increased yields of maize in recent decades. It has to be taken into account however that the interactions and the balanced combination of cropping factors (ecological, biological and agrotechnical) are the main factors which determine yields. Weather conditions were rather unfavourable during the last decade. Extreme weather conditions occurred due to global warming; 6 of 10 years were plagued by drought. Consequently yields increased from 10-20% to 30-50%. In view of varieties the situation is advantageous, maybe the supply of hybrids is too high, nevertheless those hybrids need to be selected which are particularly well adjusted to the ecological conditions. Many technologies can be applied which vary according to intensity, but the balanced combination of cropping factors should be secured on the basis of the hybrid’s intensity. Among agrotechnical factors the compensation of nutrients and technological conditions were inadequate. These days farmers only use nitrogenous fertilizers consequently they significantly decrease the easily available P and K content of the soil which in long term leads to the deterioration of the soil’s productivity. The technological background is therefore important, because sufficient yields can only be expected if agrotechnical operations are carried out in the right time and quality.
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CO2 emission of the soil on barley stubble
95-102Views:100In the last decades the physical and biological status of the soils in Hungary significantly decreased. The degree and intensity of CO2-production of the soil is in close correlation to its structural status and organic matter content. In a complex soil tillage experiment at Karcag in situ measurements have been carried out since 2002 in order to determine the CO2-emission of the soil. Carbon-dioxide emission of the soil in the cases of conventional tillage and reduced cultivation system was analysed in a long-term cultivation experiment. The measurements were carried out after the harvest of the barley, thus root respiration was excluded. For the spatial delimitation of the measuring area a newly developed frame+bowl set was used. Based on measurements, significant differences between cultivation systems can be recognized due to the soil structure changes and its effects
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Agri-Environmental Issues in the National Sustainable Development Strategy of the Member States of the Enlarged EU
346-353Views:75In preparation for the World Summit on Sustainable Development (Johannesburg), almost all EU member-states and some accession countries presented their national strategies for sustainable development (NSSD) during the summer of 2002. Along with other countries, Hungary is only now setting up this important document. Everywhere during the last decade, as in the EU countries, the intention was to involve the environmental problems into other political questions (agricultural, energy and transport policy, etc.). Today the strategy-builders have given up the sectoral view because of difficulties and setbacks, and use a view aiming the problem.
Agri-environmental issues appear as the elements of sustainability in the national strategies. For example, in accordance with the danger of global warming, the emission of green-house gasses of agriculture; in line with eco-efficiency, the production of renewable raw materials and energy sources originating from agriculture, and, in relation to changing lifestyle, food-safety and the future of biological agriculture will be disputed. With this study, I investigate the main characteristics of sustainable development strategies at both the EU level and at the level of the different member states from an agri-environmental point of view. -
Possibilities of Weed Beet (Beta vulgaris, L. ssp. marritima) Regulation
133-136Views:87Weed infestation regulation in sugar beet belongs to the most difficult growing measures, the aim of our work was to find out, if active substances contained in preparations Roundup and Fazor influence number, weight and germination of clusters in plants of weed beets. Statistically significant differences in germinability were found among control and all variants in which preparation Fazor was applied. The Roundup treatment did not have significant influence on germination. Presented results are only preliminary and one-year results, more experiments are necessary to determine the effects of the studied applications.
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Complexity of ecosystem services in agricultural fields, in particular the biodiversity
43-51Views:111Since the Convention on Biological Diversity a lot of papers have been published how to measure and value biodiversity. In the last decades publications on agro-ecosystems become more frequent and play a significant role in the provision of ecosystem services. There is a uniform definition for biodiversity in general, however, in terms of agro-ecosystems and their services (including biodiversity) many weaknesses can be identified. The objective of this paper is to explore some of these problems with special regard to different definitions and terms and to the farmland ecosystem services. One solution could be to adopt a more complex system which has some ecological and environmental components (air, water and soil pollution) and also takes in to consideration the efficiency of agricultural production.
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Grain yield and quality of maize hybrids in different FAO maturity groups
126-131Views:64An improvement in the quality of maize grain by increasing the level of components responsible for its biological value is possible
by using genetic means. However, a change in the genotype, together with improving the nutrient properties of the grain, also has some
adverse consequences connected with a fall in yield and in resistance to diseases.
Field experiments were conducted during three years (2003, 2004and 2005) to evaluate environmental effects on grain yield and
quality responses of maize hybrids. Twenty one hybrids of various maturity groups (FAO 150-400) were planted to achieve an optimum
(60-70 000 plants per hectare) plant populations and grown under the medium-N (80 kg N ha-1) fertilization. Environmental conditions
significantly affected maize hybrid responses for grain yield, starch, oil and protein contents, and consequently, starch, oil and protein
yields per hectare. Hybrids of flint type, which have a short vegetation period, had high protein and oil content but the yield averages
were low due to the slower rate of starch incorporation. Hybrids of the dent type have a longer growing season and more intense
carbohydrate accumulation, but low protein and oil contents. In wet years there was a higher rate of starch accumulation, while dry
years are favorable for protein and oil accumulation. Positive correlation existed between starch content and grain yield and 1000-
weight as well as between oil content and volumetric weight among tested hybrids. Negatively correlation existed between grain oil and
starch content as well as between oil content and grain yield and 1000-weight. Thus, end-users that require high quality maize may need
to provide incentives to growers to off set the negative correlation of grain yield with oil and protein content. -
The examination of poultry feather digestility for biogas production
113-118Views:68The aim of this research was the elaboration of the technological parameters of biological digestion and biogas production from poultry feather produced in large quantities by slaughterhouses. Feather protein was digested by Bacillus licheniformis, keratin desintegrator bacteria. Investigations focused on the optimalization of parameters influencing poultry feather biodegradation. The optimal range of pH, temperature, feather size and bacillus:feather ratio were determined in the experiments as well as the analysis of relationship between the examined parameters. In order to be able to track the dinamics of the biodegradation, we determined the extintion level of the liquid phase of the biodegraded material in the different experimental treatments. The results showed that the rate of hydrolisis was significantly higher in the treatments with bacteria than in the treatments without it. The most extensive digestion were observed in case of 1:3 feather:water ratio. The highest intensity of feather digestion were detected in the treatment with 1% microbe ratio.
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Application of AFLP-Method in Plant Sample Identification
207-213Views:62One possible method for the determination of DNA-polymorphism is the PCR-based AFLP (Amplified Fragment Length Polymorphism). This method had been succesfully introduced to the Department of Botany at University of Debrecen in 2000-2001 with the examination of hay saffron (Crocus sativus L.) and its allies. Hay saffron is grown as a spice for some thousand years producing the most expensive spice in the world. This plant is sterile, triploid reproduces only vegetatively with no fertile seeds. However its origin is unknown it exists only in cultivation and it is a mutated variety of another species or an artificial or natural hybrid. Usual methods for the systematic examination are restricted hence it seemed to be reasonable to apply molecular biological methods in its case. Results of this work include the introduction and many fold application of the method beside ensuring the consequences of science literature with determining the C. cartwrightianus to give the most similar genetical pattern to C. sativus.
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The Biology, Ecology and Medicine Faculty of Dnipropetrovsk National University after Oles’ Gonchar
15-17Views:67The historical rewiev of Biology, Ecology and Medicine Faculty is presented. The Faculty of Biology, Ecology and Medicine has 7 Departments, Aquarium complex, Zoological Museum, Vivarium and Herbarium. It works in cooperation with the Research Institute of Biology, Botanical Garden, O. L. Bel’gard International Biosphere Station, and Biological Station of DNU and forms the regional Centre of Science, Education and Culture in the field of Biology, Ecology and Nature Conservation in Central Ukraine. The Faculty proposes courses in the following specialities: Biology, Zoology, Botany, Microbiology and Virology, Biochemistry, Physiology, Ecology, Environmental Protection and Balanced Nature Management. All of them have the highest IV level of accreditation. Students get a pedagogical education.
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Studies of plant density increase – on maize hybrids of various genotypes on chernozem soil
87-92Views:137The yield and crop safety of maize are influenced by numerous ecological, biological and agrotechnical factors. It is of special importance to study one of the agrotechnical elements, the plant density of maize hybrids, which is influenced by the growing area conditions and the selected hybrid.
We have investigated the effects of three different plant numbers (50 thousand plants ha-1, 70 thousand plants ha-1 and 90 thousand plants ha-1) on the yield of 12 maize hybrids of different genotypes in Hajdúság, on calcareous chernozem soil, in the Látókép Research Farm of the University of Debrecen, Centre for Agricultural Sciences, in 2013. The experiment was set in four replications, besides commonly applied agrotechnical actions. In the experiment, 1 hybrid of very early (Sarolta), 9 of early (P 9578, DKC 4014, DKC 4025, P 9175, NK Lucius, Reseda, P 37N01, DKC 4490, P 9494) and 2 of medium (Kenéz, SY Afinity) maturation were used.
With the increase of the plant number, the number of individuals per unit area increases. According to our experimental results, we have concluded that with the increase of the plant number, the yield increased in the average of the hybrids. In the average of the hybrids, in the case of 50 thousand plants ha-1, the yield was 13 130 kg ha-1, in the case of 70 thousand plants ha-1, it was 13 824 kg ha-1, while in the case of 90 thousand plants ha-1, the yield became 13 877 kg ha-1.
In addition to plant density increase, it is necessary to determine the optimal plant number that is the most favourable for the certain hybrid under the given conditions. To fulfil this aim, we have determined the optimal plant number corresponding to the maximum yield of the given hybrid, within the given plant number range. The optimal and applied plant numbers differ, since the optimal one could only be applied under ideal conditions. Since the agrotechnical actions cannot always be carried out in appropriate quality and one has to adapt to the weather conditions, thus we have determined a plant number range in the case of each hybrid. The hybrids were classified into categories of producible in narrow and broad plant number range.
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The potential of biological control on invasive weed species
73-75Views:41Sorghum halepense is one of the invasive species in Europe. This study was made to identify the morphology of fungi on invasive weed species samples on the roots of Sorghum halepense. The samples were collected in the region of Debrecen. The experiment was conducted under laboratory conditions to determine the microscopic form of fungi. The samples were put on PDA and for identification of fungi is based on the morphological characteristics of the features and colonies of conidia that were developed in Petri dishes.
The examination of the culture revealed that the fungus from the root of Sorghum halepense was Aspergillus niger. Pathogenicity and the relationship between the fungus and Sorghum halepense are still uncertain so in the future pathogenicity tests and re-isolations from plants are very important steps.
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The bioactive compounds of sour cherry (Prunus cerasus) with special regards to antioxidant activity and antioxidant density
83-87Views:165Evidence suggests that a diet high in fruits and vegetables may reduce the risk of chronic diseases, such as cardiovascular disease and cancer, and phytochemicals including phenolics, flavonoids and carotenoids from fruits and vegetables may play a key role in reducing chronic disease risk. Recent research has proven that sour cherry (Prunus cerasus L.) is a valuable natural source of some bioactive compounds important in human health preservation.
In our work, we identified the total antioxidant capacity and ”antioxidant density” of sour cherry varieties named ”Újfehértói fürtös”, ”Debreceni bõtermõ”, ”Kántorjánosi” and ”Érdi bõtermõ” and those of the ”Bosnyák” sour cherry clones. ”Antioxidant density” is a biological value indicator obtained in a synthetic way, which indicates the antioxidant capacity of the particular food, e. g. fruit and vegetable, per 1 Calorie. -
Preliminary estimation of the efficacy of Fusarium sporotrichioides Sherb. as biological control agent against common milkweed (Asclepias syriaca L.)
201-204Views:248A study of fungi responsible for severe leaf spots of common milkweed (Asclepias syriaca L.) in the Hajdúság region (East Hungary), Fusarium sporotrichioides and Alternaria alternata were isolated from infested leaf tissues. F. sporotrichioides was the most virulent fungus in pathogenicity tests conducted on healthy leaves of common milkweed plants. Inoculation of common milkweed (A. syriaca) in different growth stages with F. sporotrichioides yielded similar symptoms as the original ones. Spray mixtures containing 1.0×106 conidia/ml gave effective control when common milkweed plants were sprayed until runoff occurred. Laboratory (wet chamber) and field experiments showed that asexual spores of the fungal pathogen, F. sporotrichioides, exhibited bioherbicidal activity against common milkweed (A. syriaca).
More efficient control efficacy was observable on elder plants (at flowering stage) than younger ones. These results initiate that this fungus may be a biocontrol agent for controlling this invasive weed but should clarify its hosts because it could infect cultivated plants as well.
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The effect of lead and copper heavy metal salts on soil microorganisms under laboratory circumstances
55-59Views:137he population dynamics of calcareous chernozem soils polluted with different concentrations of lead and copper heavy metal saline solutions was examined.
The experiment was carried out in the soil biological laboratory of the Institute of Agricultiral Chemistry and Soil Science at DE AGTC MÉK in 2012. For the determination of the concentration of the undiluted stock solutions we multiplied the smallest toxic concentration values of the MSZ 08-1721/1-86 Hungarian standard by forty. The intermediary concentrations of the treatments were produced with adequate dilution of the stock solutions until a dilution level equal to the values of the standard. The statistical evaluation of the data was performed with ANOVA (Analysis of Variance) including the determination of the standard deviation and significant difference. Investigating the effects of the different treatments on the soil microbes we established that both heavy metal saline solutions had a negative effect on the population dynamics of bacteria and microscopic fungi living in the soils. The negative effect of copper – as a potential toxic micro nutrient – turned out to be less strong than the negative effect of the toxic lead. According to our results the correction of the treatment levels is recommended in order to further tolerance examinations and the determination of the tolerance levels.
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Studies of Expression of Peptaibol Synthetase of Trichoderma reesei
188-190Views:117Because of the potential importance of peptaibols in the biological control of plant diseases, a transgenic, a T. reesei strain carrying a tex1-promoter: goxA fusion plasmid was constructed for furthur studies. The peptaibol synthetase gene (which is highly similar to T. virens tex1) was identified in the genome sequence of T. reesei. A 900 bp 5’ upstream noncoding fragment, presumed to include the promoter region of tex1, was cloned into the pSJ3 plasmid (which contains the Aspergillus niger goxA gene encoding glucose oxidase). Finally, we transformed T. reesei with the tex1-promoter: goxA fusion containing pSJ3 plasmid.
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Fusarium culmorum isolated from rhizosphere of wooly cupgrass (Eriochloa villosa) in Debrecen (East Hungary)
93-96Views:143Wooly cupgrass (Eriochloa villosa) is an East-Asian originated weed species and it has been spreaded worldwide by now. The first occurrence of this species in Hungary was observed and published in 2008 nearby Gesztely village (Borsod-Abaúj-Zemplén county, North-East Hungary) than in the summer of 2011 a significant population was discovered next to Debrecen city (Hajdú-Bihar county, East Hungary).
In 2013 this weed was also reported from Szentborbás village, Somogy county (South-West Hungary). These observations of spreading and its biological features (production of stolons and large number of seeds, moreover herbicide tolerance) indicate that wooly cupgrass (E. villosa) has a great potential of invasiveness, so it may become a hazardous weed not only in Hungary but in all over the world.
The objective of this study was to identify the fungus which was isolated from wooly cupgrass (E. villosa) root residue samples which were collected after maize harvesting on arable land in late autumn, near Debrecen. The identification of the fungus based on morphological characters of colonies and the features of conidia developed on potato dextrose agar (PDA) plates. After the examination of axenic culture we revealed that the fungus from rhizosphere of wooly cupgrass was Fusarium culmorum. Pathogenicity and/or endophytic relationship between the fungus and wooly cupgrass is still uncertain so pathogenicity tests and reisolations from plants are in progress.
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Analysis of the photosynthetic parameters, the yield and the quality of winter wheat
101-106Views:125The environmental adaptability of crop production is basically determined by the selection of biological background (plant species and
varieties) suitable for the region and the site. The aim of our work is to parametrize the plant assimilation, its intensity, dynamics and the
most important characteristics and the relationships to the quality in winter wheat trials. The measurements were carried out at the research
site of the University of Debrecen in small parcel experiments. We measured the leaf net CO2 assimilation rate, stomatal conductance,
intercellular CO2 level, the transpiration, the leaf temperature and the air temperature by the LICOR LI-6400 portable photosynthesis
system in field trials on the nutrient supply. The soil of the experimental area is calciferous chernozem with favorable water regime.
We have examined the photosynthetic activity, the productivity and yield stability of winter wheat varieties. We have compared the yield
results, at similar agrotechnical conditions in seven cropyears. We also determined the quality parameters of the winter wheat varieties.
Then we valued the yield stability of genotypes with the help of analysis of variance and linear regression equations. We have defined the
connections between assimilation parameters, the yield stability and quality parameters of wheat varieties.