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The bioactive compounds of sour cherry (Prunus cerasus) with special regards to antioxidant activity and antioxidant density
83-87Views:165Evidence suggests that a diet high in fruits and vegetables may reduce the risk of chronic diseases, such as cardiovascular disease and cancer, and phytochemicals including phenolics, flavonoids and carotenoids from fruits and vegetables may play a key role in reducing chronic disease risk. Recent research has proven that sour cherry (Prunus cerasus L.) is a valuable natural source of some bioactive compounds important in human health preservation.
In our work, we identified the total antioxidant capacity and ”antioxidant density” of sour cherry varieties named ”Újfehértói fürtös”, ”Debreceni bõtermõ”, ”Kántorjánosi” and ”Érdi bõtermõ” and those of the ”Bosnyák” sour cherry clones. ”Antioxidant density” is a biological value indicator obtained in a synthetic way, which indicates the antioxidant capacity of the particular food, e. g. fruit and vegetable, per 1 Calorie. -
Preliminary estimation of the efficacy of Fusarium sporotrichioides Sherb. as biological control agent against common milkweed (Asclepias syriaca L.)
201-204Views:248A study of fungi responsible for severe leaf spots of common milkweed (Asclepias syriaca L.) in the Hajdúság region (East Hungary), Fusarium sporotrichioides and Alternaria alternata were isolated from infested leaf tissues. F. sporotrichioides was the most virulent fungus in pathogenicity tests conducted on healthy leaves of common milkweed plants. Inoculation of common milkweed (A. syriaca) in different growth stages with F. sporotrichioides yielded similar symptoms as the original ones. Spray mixtures containing 1.0×106 conidia/ml gave effective control when common milkweed plants were sprayed until runoff occurred. Laboratory (wet chamber) and field experiments showed that asexual spores of the fungal pathogen, F. sporotrichioides, exhibited bioherbicidal activity against common milkweed (A. syriaca).
More efficient control efficacy was observable on elder plants (at flowering stage) than younger ones. These results initiate that this fungus may be a biocontrol agent for controlling this invasive weed but should clarify its hosts because it could infect cultivated plants as well.
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The effect of lead and copper heavy metal salts on soil microorganisms under laboratory circumstances
55-59Views:137he population dynamics of calcareous chernozem soils polluted with different concentrations of lead and copper heavy metal saline solutions was examined.
The experiment was carried out in the soil biological laboratory of the Institute of Agricultiral Chemistry and Soil Science at DE AGTC MÉK in 2012. For the determination of the concentration of the undiluted stock solutions we multiplied the smallest toxic concentration values of the MSZ 08-1721/1-86 Hungarian standard by forty. The intermediary concentrations of the treatments were produced with adequate dilution of the stock solutions until a dilution level equal to the values of the standard. The statistical evaluation of the data was performed with ANOVA (Analysis of Variance) including the determination of the standard deviation and significant difference. Investigating the effects of the different treatments on the soil microbes we established that both heavy metal saline solutions had a negative effect on the population dynamics of bacteria and microscopic fungi living in the soils. The negative effect of copper – as a potential toxic micro nutrient – turned out to be less strong than the negative effect of the toxic lead. According to our results the correction of the treatment levels is recommended in order to further tolerance examinations and the determination of the tolerance levels.
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Studies of Expression of Peptaibol Synthetase of Trichoderma reesei
188-190Views:117Because of the potential importance of peptaibols in the biological control of plant diseases, a transgenic, a T. reesei strain carrying a tex1-promoter: goxA fusion plasmid was constructed for furthur studies. The peptaibol synthetase gene (which is highly similar to T. virens tex1) was identified in the genome sequence of T. reesei. A 900 bp 5’ upstream noncoding fragment, presumed to include the promoter region of tex1, was cloned into the pSJ3 plasmid (which contains the Aspergillus niger goxA gene encoding glucose oxidase). Finally, we transformed T. reesei with the tex1-promoter: goxA fusion containing pSJ3 plasmid.
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Fusarium culmorum isolated from rhizosphere of wooly cupgrass (Eriochloa villosa) in Debrecen (East Hungary)
93-96Views:143Wooly cupgrass (Eriochloa villosa) is an East-Asian originated weed species and it has been spreaded worldwide by now. The first occurrence of this species in Hungary was observed and published in 2008 nearby Gesztely village (Borsod-Abaúj-Zemplén county, North-East Hungary) than in the summer of 2011 a significant population was discovered next to Debrecen city (Hajdú-Bihar county, East Hungary).
In 2013 this weed was also reported from Szentborbás village, Somogy county (South-West Hungary). These observations of spreading and its biological features (production of stolons and large number of seeds, moreover herbicide tolerance) indicate that wooly cupgrass (E. villosa) has a great potential of invasiveness, so it may become a hazardous weed not only in Hungary but in all over the world.
The objective of this study was to identify the fungus which was isolated from wooly cupgrass (E. villosa) root residue samples which were collected after maize harvesting on arable land in late autumn, near Debrecen. The identification of the fungus based on morphological characters of colonies and the features of conidia developed on potato dextrose agar (PDA) plates. After the examination of axenic culture we revealed that the fungus from rhizosphere of wooly cupgrass was Fusarium culmorum. Pathogenicity and/or endophytic relationship between the fungus and wooly cupgrass is still uncertain so pathogenicity tests and reisolations from plants are in progress.
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Analysis of the photosynthetic parameters, the yield and the quality of winter wheat
101-106Views:125The environmental adaptability of crop production is basically determined by the selection of biological background (plant species and
varieties) suitable for the region and the site. The aim of our work is to parametrize the plant assimilation, its intensity, dynamics and the
most important characteristics and the relationships to the quality in winter wheat trials. The measurements were carried out at the research
site of the University of Debrecen in small parcel experiments. We measured the leaf net CO2 assimilation rate, stomatal conductance,
intercellular CO2 level, the transpiration, the leaf temperature and the air temperature by the LICOR LI-6400 portable photosynthesis
system in field trials on the nutrient supply. The soil of the experimental area is calciferous chernozem with favorable water regime.
We have examined the photosynthetic activity, the productivity and yield stability of winter wheat varieties. We have compared the yield
results, at similar agrotechnical conditions in seven cropyears. We also determined the quality parameters of the winter wheat varieties.
Then we valued the yield stability of genotypes with the help of analysis of variance and linear regression equations. We have defined the
connections between assimilation parameters, the yield stability and quality parameters of wheat varieties. -
Effect of plant extract against opportunist human pathogen soil bacteria
89-93Views:99Our experiments have repeatedly shown that the extract of ragweed (Ambrosia artemisiifolia) is an antifungal and antibacterial component.
In our paper we tested of the biological activity of the herbal substance against opportunistic human pathogenic bacteria strains (Staphylococcus
aureus 110003 and 25923; Staphylococcus saprophyticus 110008). Our laboratory tests show that the extract is bacteriostatic and in several cases bactericid. We can assume that from the the agricultural and public health aspects can be extremely dangerous weed, contains biologically active components and it may be suitable for the prepare of antibacterial agents. -
The effect of various composts on vegetable green mass on two soil types
179-183Views:136Composting of sewage sludges makes easier the utilization of sewage sludge in the agriculture and the composts in good quality could increase the nutrient content of soil. Due to the composting process, the sewage sludge composts with high organic matter content can be utilized in the same way as other composts or farmyard manure.
Composts produced in different ways have different effects on the physical, chemical and biological properties of different soils, although their positive effects have already proved in the literature. In our study the effects of composts from different composting processes were investigated in soil-plant systems. The different physical and chemical properties of the two examined soil types (arenosol and chernozem)strongly influenced the nutrient supply capacity of composts which could be characterized by the growth of ray-grass as a test plant in the pot experiment. In this work we examined the effects of three different composts on the green weight of plants on the fourth and eighth weeks after the treatment and sowing. -
Microbiological and Chemical Characterization of Different Composts
106-111Views:68Composting of agricultural waste is considered particularly important from the point-of-view of environmental protection. Degradation of organic substance results in a significant reduction of waste volume.
The end product of the composting process, mature compost, can be used as soil coverage against excess loss of wastes, for mulching, for organic manure etc. The problem of composting has come into limelight in environmental studies and in agriculture.
The quality of the mature compost is determined by physical, chemical and biological parameters of the composting process which, in turn, depend on initial composition of the raw materials, the technology, e.g. regular mixing and moistening and on environmental factors. Quality is the key question in compost use.
We studied the composting process in compost windrows of different raw material composition. We measured temperature, humidity content, pH, organic substance content, nitrogen and carbon content.
We counted the number of bacteria, microscopic fungy, ammonifying and cellulose decomposing microorganisms. We directed the composting process with turning weekly (to provide oxygen) and watering (to provide humidity content 40-60%).
We set up windrows of 1 m3 volume from dry plant substances (cornstalk, pea straw, tomato stalk and crop, weeds) and cow manure not older than 1 week. The cow manure was used at ratios of 0%, 35%, 50%, 65% and 100%, respectively.
We measured changes in compost temperature relationship with outside temperature until they were almoust the same. Humidity was 40-60% in most cases.
At the beginning of the process, pH was slightly acidic-neutral; it later becomes neutral-slightly alkaline (pH: 6.93-8.02) as ammonia is liberated from proteins.
At the end of the process, pH decreased again, due to humification.
Organic substance content decreased as microorganisms mineralized them. Organic carbon content decreased gradually due to microorganisms used it as an energy.
Total nitrogen content increased until middle of july and decreased gradually until than.
The carbon/nitrogen rate were higher in the beginning, it decreased until july-august and increased by smaller degree until end of the process.
The number of bacteria was higher in the first three weeks and between june-september. The number of cellulose degrading bacteria was the highest in the first three month, the number of ammonifying bacteria was the highest from the end of may until sepember.
The number of microscopic fungy was significant in the second part of process, after july. -
Preface
5Views:29In the frame of a common “Hungarian-Ukrainian Intergovernmental S&T Cooperation Programme” which title is “Change of soils ecological characteristics of Ukraine and Hungary in the conditions of anthropogenic transformed ecosystems and optimization of biological processes of plants primary feeds elements mobilization” a Workshop was held in Debrecen. The member institutes of project participated with different presentation in this program.
The title of Workshop was: “Anthropogenic effect on the properties of Middle and Eastern European chernozem soils and on the sustainable agricultural production”.
The aim of the Workshop was to give relevant information about the present situation of the Middle and Eastern European Chernozem soils, especially emphasize the effect of different loading on the quality (properties) of chernozem soils. With the Workshop we would like to create a tradition for discussion about the anthropogenic effect on the soil properties and through it on the productivity of different soils. It was a forum for discussion of research results related to problems and possibilities for prevention of soil quality. With this possibility we would like to contribute to the sustainable agricultural production.
The papers were read for the publisher and we would like to show them in a separate supplement of Journal of Agricultural Sciences, Acta Agraria Debreceniensis as one of the results of the project.
The papers comply with the requirements of the scientific issue except those two which show the university and the department of the Ukrainian partner taking part in this project.
The participant Institutes of the project:
- Dnepropetrovsk National University, Faculty of Biology and Ecology;
- Kryvyi Rig Botanical Garden NAS of Ukraine, Plant Physiology & Soil Biology Department;
- Department of Agrochemistry and Soil Sciences of Centre for Agricultural and Applied Economics;
- Research Institute of Karcag, Centre for Agricultural and Applied Economics. The collaboration with Ukrainian partners was successful and we have confidence in the further cooperation in scientific research. -
Selenium speciation analysis of selenium-enriched food sprouts
23-28Views:182In this present study, we prepared selenium-enriched pea and wheat sprouts. During our research we aimed not only to measure the total selenium content of the sprouts but to identify different selenium species.
Scientifical researches show why the analytical examination of different selenium (Se) species is necessary: consumption of all kind of Se-species is useful for a person who suffers in selenium deficit, while there is significant difference between effects of different Se-species on person, in whose body the Se-level is just satisfactory. Biological availability, capitalization, accumulation, toxicity of Se-species are different, but the main difference was manifested in the anti-cancer effect of selenium.
During our research selenium was used in form of sodium selenite and sodium selenate, the concentration of the solutions used for germination was 10 mg dm-3. Control treatment meant germination in distilled water. Total selenium content of sprout samples was measured after microwave digestion by inductively coupled plasma mass spectrometry (ICP-MS). Different extraction solvents were applied during sample preparation in order to separate different Se-species (0.1 M and 0.2 M HCl or 10 mM citric acid buffer). We wanted the following question to be answered: Which extraction solvent resulted the best extraction efficiency? Selenium speciation analysis of sprout sample extracts was performed by high performance liquid chromatography with anion exchange column, detection of selenium species was performed by ICP-MS.
Evaluating our experimental results we have been found that significant amount of selenium of inorganic forms used during germination transformed into organic selenium compounds. There was difference between the amount of Se-species in pea and wheat sprouts and selenium uptake and repartition of selenium species were depended on Se-form used during germination. In addition the chromatogram analysis made us clear as well, that the citric acid solvent proved to be the most effective extraction solvent during sample preparation int he view of organic Se species.
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Investigating the above-ground biomass values of sweet potatoes (Ipomoea batatas)
35-39Views:95The role of sweet potato tubers in human nutrition is not new. The above-ground biomass of sweet potatoes is not used for nutritional purposes in most countries, but it has a high biological value. Therefore, the aim of the present study is to investigate the production of press fibre from above ground biomass by wet fractionation. Two sweet potato varieties (purple- and white-fleshed sweet potato) and two types of irrigation system were used: bubbling water flow system (BWS) and continuous water flow system (CWS). Glucan, xylan, arabinan were analysed by HPLC and elemental content was measured by ICP-OES. Our results show that the total carbohydrate content in the pressed fibre of the leaf blades (27.64–29.88 %w/w) is lower than in the stem with petiole (51.14–57.36 %w/w). No significant difference in glucan, xylan and arabinan content was observed in the leaf blade. In the stem with petiole, significant differences were observed for xylan and arabinan contents. For elemental content, generally higher values were measured in the leaf blade than in the stem with petiole. This information may be relevant for the selection of the appropriate variety and treatment, even for the production of functional food.
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Comparison of Reproductive Performance of the Roe Deer (Capreolus capreolus, L.) Among Different Regions
41-46Views:79The potential and actual number of offspring of roe deer and the difference between these figures (prenatal and postnatal loss) significantly vary in each population yearly. The objective of this study is to examine the potential and actual number of offspring, the number of losses, and to find a link between the most important biological characteristics of does (body weight – BW, condition – KFI) and the number of raised offspring on four territories on the Great Hungarian Plain.
Where the number of corpora lutea (CL) is the highest, there the losses are the highest as well, and the number of raised offspring is the lowest (region I.). Here, the rearing loss is double that of the weakest territory (region IV.). Rearing losses can be associated with the fenotype of does (BW, KFI) but environmental factors also have determinative importance. Where the number of twin-calving does was the highest, I found four times more does without a fawn than where the number of twin-calving does was the lowest. The nursing success was the best (the losses were lowest) in the region where the potential offspring (number of CL) was also the lowest, but the coverage of the habitat and the proportion of forests were largest. The food supply for the animals in autumn and winter are not enough, the structure of the habitat has to be improved as well, so that it might become adequate for game protection in extreme weather conditions.
The results have to be considered as preliminary ones. It is essential to continue and extend the research to increase the reliability of the results. -
Development alternatives of rural economy
187-191Views:100Environmental, natural, social and economic processes undergoing both in Europe and in the world predict such a 21st century that is characterised by increasing resource-crisis from both economic and ecological aspects. Therefore, it is very important for Hungary to see what happens to its natural resources, epecially to its agricultural land, water reserves as well as the biodiversity of the local unique flora and fauna. One of the most significant issues of the rural areas of Hungary is whether we can preserve the natural habitats and the various biodiversity of the related species, the favourable biological background of agriculture. In addition, whether we are able to provide high quality food for the country as well as for the broader reagion, whether we are able to produce energy from the resources available as well as to provide sufficient opportunities for the population to live and work. These can be considered as the most significant issuesof the coming decades which determine the strategy of the Hungarian rural economies in long term.
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Saccharomyces cerevisiae growth kinetics study dairy byproduct
169-172Views:165By guess, annual volume of milk whey is 185–190 million tons and this volume probably will increase next years. Whey has significant biochemical oxygen demand due to its high organic matter content so whey as sewage is one of the most pollutant by-products in the food industry. Apart from environmental pollution, benefit of several whey constituents for human health is another reason to utilize whey. Corn and potato, as well as the processing of milk in the food industry in large quantities of by-products generated by low cost, substantial quantities of starch and lactic acid, which are due to high biological oxygen demand are considered as hazardous waste. Some of them are destroyed sewage storage tanks, and those products are excellent substrates for the growth of microorganisms could be. The traditional nutrient solution optimization methods are solution and time-consuming and are not able to determine the real optimum because of the interaction of factors involved.
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Introduction of DNA-based Methods to Agriculture Through Molecular Taxonomic Examination of Poa Species
139-142Views:83biological methods, one among them is AFLP that is well applicable for taxonomic research. Bluegrass species, that are important components of meadow associations, thus their thorough knowledge is necessary in maintaining biodiversity, were examined with bringing this method to perfection.
Taxonomic relationship of the members of Poa pratensis aggregation is a controversial issue. Present study aimes to identify the members of this group, with a developed AFLP method through molecular taxonomic examination of Poa species in meadows nearby Debrecen, revealing their genetical distances. Species of the aggregation show a great genetic variability, but their genetic proximity approves the use of the term aggregation. Results established wider geographical investigation of three species of the aggregation. The distinctness of the species based on their morphological features was confirmed according to their genetical basis as well. The method overall turned out to be appropriate for the taxonomic research of bluegrass species like Poa pratensis and its aggregation. -
Methods for detention of lipid rancidity
117-120Views:198There are various methods available for measurement of lipid oxidation in foods.Changes in chemical, physical, or organoleptic properties of fats and oils during oxidation may be monitored to assess the extent of lipid oxidation. However, there is no uniform and standard method for detecting all oxidative changes in all food systems. The available methods to monitor lipid oxidation in foods and biological systems may be divided into two groups. The first group measures primary oxidative changes and the second determines secondary changes that occur in each system.
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The dynamics of biodiversity structure of soil microorganisms under the impact of biopreparations during potato growing season
67-74Views:141The use of biological preparations such as Phytotsid and Planryz contributes the increase of the general number of soil bacteria population by 13.0–36.1% in the case of potato variety Scarbnytsya and by 4.5–24.6% for potato variety Oberig compared with control. It also increases the number of saprophyte microflora, which compete with plant pathogens, micromycetes, and causes 1.2–1.8 times reduction in the number of soil fungi – Fusarium and Alternaria. During the application of Rovral Akvaflo the Shannon ecological index of species biodiversity is lower than during the biopreparation use. The decrease of species biodiversity was observed as well as strengthening the dominance of some species (dark pigmentation in fungi).
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The examination of the agronomy, the amount of yield, and the yield stability of winter wheat varieties
61-72Views:78Our research was carried out at University of Debrecen Centre for Agricultural Sciences Faculty of Agriculture Institution of Plant Sciences Látókép Research Institute through the breeding year of 2003/2004, 2004/2005 and 2005/2006 using cherrnozem soil. In our research we tested 14 chosen autumn wheat varieties during the three crop years.
The different varieties showed very dissimilar ability of resistance against diseases through the three crop years. We could observe both susceptible and resistant varieties. Susceptible varieties got diseases even in favourable crop years. The observed winter wheat varieties showed higher susceptibility against helminthosporium (21.8%) and leaf rost (16.4%). Among the 14 varieties we experienced the least susceptibility in the case of ‘Gaspard’ and ‘GK Kalász’. The research showed that the disease of fusarium undoubtedly depends on the features of the crop year.
In terms of stem solidity we experienced big differences. Among all the observed winter wheat varieties the mid-late ripening ‘Gaspard’ showed the best results in the average of the three years, only 5.3% was beaten down.
The three ripening group of the winter wheat showed the following average yield in the average of three years: 7065 kg/hectare (early ripening varieties), 7261 kg/hectare (late ripening varieties), 6793 kg/hectare (mid-late ripening varieties). Among all the observed varieties the early ripening ‘Flori 2’ produced the biggest yield (7692 kg/hectare).
During the three crop years we reached very different amounts of yield which means that weather conditions had a telling affect on yield. In 2004 we reached an excellent average yield in all the tree breeding groups because of the favourable weather conditions. In 2005 we had a moderate amount of yield because of the unfavourable weather conditions of winter. The year of 2006 showed the smallest amount of yield which is due to the fact that the plant grew less thick than usually.
There were significant differences among the observed varieties in the term of yield, which can be attributed to dissimilar biological basics.
One of he most important questions is the yield stability of the varieties. We had extremely different results at this field. Speaking in general terms we can state that both weather conditions and genetical abilities have a determining effect on yield. In the case of winter wheat varieties the rate of yield fluctuation was quite big, moving in the interval of 33.7-70.3%. Among all the observed varieties ‘Gaspard’ showed the best yield stability (33.3%). -
The effect of plant density to the yield results and the yield components of maize hybrids
89-93Views:133Maize is the crop that is produced on the second largest area in our country, in Hungary. It is planted on nearly 25% of the country’s growing area and it was produced on 1 090 439 hectares in 2016. Despite the continuous development of the biological basis and production
technology, the growth of the yield results is not constant, its fluctuation is significant. It can be even up to 60%, because of the extremity of the years. The exploitation of the yield potential of modern hybrids is possible if we harmonize the effects of the ecological factors and properly applied instruments of agro technology and by these we ensure their interaction to reach a favorable outcome. The applied plant density is an important, well researched, but at industrial level a not enough utilized element of the maize production.
The results of the extensive tests, done between 2009 and 2015, showed that the genotype, the year effect and the plant density are in strong correlation with each other determining the yield results. In the past seven years the examined genotypes reached the highest yield
performance at the highest plant densities. The early hybrids (RM90–95, FAO 200–300) are capable of producing them at higher plant density, while in case of the mid and late maturity varieties the further increasing of the density after reaching the optimum level led to yield depression.
According to our experimental results, the yield is in close positive correlation with the increase of the plant density. The effect of the growing season has great significance in forming the yield results and this determines the applicable plant density too.
The yield of maize is determined by a resultant of components. The main component is the number of ears per plant and the amount of kernels per ear, which is calculated from the number of kernels on an ear and the weight of them. The number of the kernels on an ear is
calculated from the number of rows on the cob multiplied by the number of seeds in one row on the cob. In dry years, at lower yield levels the yield decreases because of the shorter ears, while at the higher levels the number of kernels in a row and the thousand-kernel weight decreases,causing yield depression this way. From our examinations it turned out that the plant density reaction of a genotype is individual, every variety reaches its maximum kernel number per hectare – in other words the maximum yield - in an individual way. -
Heavy Metals in Agricultural Soils
85-89Views:69The soil constitutes the basis of the food chain. To keep soil conditions in a good trim is very important, it’s part of the sustainable development and of producing food supply harmless to health.
In some cases, soil productivity is the only important part, qualitative requirements or economical characteristics can improve it. The soil is threatened by two danger factors: the soil degradation and the soil pollution. The accumulation of different harmful and/or toxic substances in the soil is well known. Heavy metals constitute a part of it. Metals in the soil and in the soil-solution are balanced. This balance depends on the type of the metal, on the pH, on the cation-band capacity of the soil, on the redox relations and the concentration of cations in the soil.
To be able to handle the metal contamination of the soil, it is important to estimate the form, the possible extension and the concentration of metals.
Of course, the different types of soils have different physical-chemical, biological and buffer capacity, they can moderate or reinforce the harmful effects of heavy metals. To draw general conclusion of the dispersion and quantitative relations on the metals originated from different contamination sources is hard, because in some emissive sources contamination is limited in small areas but on a high level, some others usually expand on larger areas, and as a result of equal dispersion, the contamination’s level is lower.
Heavy metals – unlike alkali ions – strongly bond to organic materials, or infiltrate in a kelát form. Their outstanding characteristic is the tendency to create metal-complex forms. Kelats take part in the uptaking and transportation of heavy metals. Heavy metals exert their effects mostly as enzyme-activators.
The metals cannot degrade in an organic way, they accumulate in living organisms, and they can form toxic compounds through biochemical reactions.
Lot of the heavy metals accumulate on the boundaries of the abiotic systems (air/soil, water/sediment), when physical or chemical parameters change, and this influences their remobilization.
Human activity plays a great part in heavy metal mobilization, results in the human origin of most biochemical process of metals.
To understand the toxic influence of accumulated metals of high concentration, their transportation from soils to plants or their damage in human health, must clearly defined and investigated.
For effective protection against soil pollution, the types and levels of harmful pollution to soil must identified, regarding legal, technical and soil-science aspects, preferable in a single way. Difficulties in this area mean that toxicity depends on loading, uptake, soil characteristics and living organisms (species, age, condition etc.), furthermore, local and economic conditions considerably differ. -
Study of the biodegradation of slaughterhause feather waste by Bradford method
77-81Views:119The 15–20% of the by-products of meat- and poultry industry – that unsuitable for human consumption – contains keratin. The slaughter technology of poultry produces large amount of poultry feather with 50–70% moisture content. This means more million tons annually worldwide (Williams et al., 1991; Hegedűs et al., 1998). The keratin content of feather can be difficulty digested, so physical, chemical and/or biological pre-treatment is needed in practice, which has to be set according to the utilization method. The microbiological and enzymatic degradation of feather to soluble protein and amino acids is a very favourable and relatively cheap opportunity to produce valuable products of the resulting feather. Our applied treatments were based on the determination of the most effective method, which is able to follow the biodegradation of waste poultry feather.
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Inhibition of the spread of Sclerotinia sclerotiorum in aquaponics
5-8Views:200Sclerotinia sclerotiorum, which causes white mold, is a widespread pathogen. In 2020, a new host plant of this fungus, the watercress (Nasturtium officinale) was identified in Hungary in an aquaponic system. During the cultivation of watercress S. sclerotiorum was detected on the plant, the fungus caused a 30% yield loss. Fungicides should not be used against fungi in aquaponic systems. Non-chemical methods of integrated pest management should be used. These include biological control (resistant species, predators, pathogens, antagonist microorganisms), manipulation of physical barriers, traps, and the physical environment. In the aquaponic system, the removal of the growing medium (expanded clay aggregate pellets) solved the damage of Sclerotinia sclerotiorum 100%. By removing the expanded clay aggregate pellets, the environmental conditions became unfavorable for the development and further spread of the S. sclerotium fungus.
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Application of the SDS-PAGE method for the characterisation of winter wheat flour quality
112-118Views:101The principle, development and importance of the SDS-PAGE method are presented in this article. The SDS-PAGE method has become one of the basic methods of molecular biological research, because it is widely applicable and its sensitivity is excellent in the separation of wheat storage proteins.
We have shown the application of this method with a concrete example. It was also tested whether, it was possible to obtain a better baking quality product from a large amount of poor quality less valuable wheat by fractioning the flour according to particle sizes after grinding. We studied the rheological properties of flours with different particle size fractions from the original flour. The baking quality of the original flour was B2. The 125-90 and 90-63 μm fractions have significantly better baking quality (B1) than the original flour. The protein contents of these flour fractions were also significantly higher than the protein content of the original flour. We had a question: what has influenced the baking quality: the protein content or other factors? We searched for an explanation on these results in the protein composition of the flour samples. We studied the distribution of glutenin-fractions by SDS-PAGE method and evaluated them. We found with correlation analysis that the amount of LMW-Glutenin D-group (52-60 kDa) is in a strong, negative correlation to the baking quality (r = – 0.855*). Therefore, the baking quality of flour samples was influenced by this glutenin fraction. -
Preservation of Biological Diversity of Domestic Animals in Hungary
18-29Views:95Since the 1992 Rio de Janeiro UNO Congress domestic animals belong also officially to the genetic diversity of the world. Non commercial domestic animal breeds should be maintained for many cultural and technical reasons. Conservation and preservation of living beings is nowadays in the programme of many regional, national and international organizations.
The preservation of domestic animals is possible in situ (at the original place and conditions) and ex situ (by cryogenic methods).
There are three era in the history of preservation of domestic animals: the epoch of spontaneous maintenance, the period of sporadic national activities and the era of international programme.
Some of the questions to be solved by scientific research: the principles of selection of the candidate populations for maintenance, the different degrees of endangeredness, the necessary population size to be subsidized, the problem of inbreeding, the best mating systems etc.
In Hungary the maintenance of endangered domestic animal breeds is based upon the low.
The following breeds are on the list of protected breeds:
− the Hungarian Grey cattle,
− the Lipizzan, Shagya, Nonius, Gidran, Furioso,Kisbér Halfbred, Murinsulaner and Hucul horses,
− the Racka, Tsigai and Cikta sheep,
− the Mangalitsa pig,
− the Hungarian yellow, white, speckled and the Transsylvanian naked necked hen,
− the Bronze Turkey,
− the Frizzle Feathered goose.
Hopefully in the near future the breeders of traditional domestic animal breeds will find the possible niches for their special products.