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Application of the SDS-PAGE method for the characterisation of winter wheat flour quality
Published May 23, 2006
112-118

The principle, development and importance of the SDS-PAGE method are presented in this article. The SDS-PAGE method has become one of the basic methods of molecular biological research, because it is widely applicable and its sensitivity is excellent in the separation of wheat storage proteins.
We have shown the application of this method wi...th a concrete example. It was also tested whether, it was possible to obtain a better baking quality product from a large amount of poor quality less valuable wheat by fractioning the flour according to particle sizes after grinding. We studied the rheological properties of flours with different particle size fractions from the original flour. The baking quality of the original flour was B2. The 125-90 and 90-63 μm fractions have significantly better baking quality (B1) than the original flour. The protein contents of these flour fractions were also significantly higher than the protein content of the original flour. We had a question: what has influenced the baking quality: the protein content or other factors? We searched for an explanation on these results in the protein composition of the flour samples. We studied the distribution of glutenin-fractions by SDS-PAGE method and evaluated them. We found with correlation analysis that the amount of LMW-Glutenin D-group (52-60 kDa) is in a strong, negative correlation to the baking quality (r = – 0.855*). Therefore, the baking quality of flour samples was influenced by this glutenin fraction.

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Occurrence of woolly cupgrass (Eriochloa villosa /Thunb./ Kunth) in Hajdú-Bihar county, Hungary
Published October 30, 2011
119-123

Woolly cupgrass (Eriochloa villosa /Thunb./ Kunth) is native to East Asia, it spreads in several parts of the World and causes difficulties in plant protection, especially in maize. Difficulties in control of Eriochloa villosa originated from several reasons: seeds continue to germinate later in the season, significant part of seeds emerges fro...m a deep layer of the soil, and the species is less susceptible to some herbicides applied to maize than other annual grass weeds.
The first report on the occurrence of woolly cupgrass in Hungary was published in 2008, and it reported about the appearance of this species near to Gesztely village (Borsod-Abaúj-Zemplén county), however, no information has been added about spread of the weed in Hungary until now.
A significant population was discovered next to Debrecen (Hajdú-Bihar county) in summer, 2011, and then weed associations were examined in maize, sunflower and stubble-fields on several km2 in the area to estimate the Eriochloa villosa infection. The weed species was found on every maize field bordering with a ground cover of 0.5-4%. Woolly cupgrass occurred inside of the 50% of maize fields, and reached a ground cover of 76% in case of most infected area, in addition it was found in sunflower and stubble-fields.
The spread of woolly cupgrass is expected in this area, which requires the consideration of this species in the planning of weed management technologies.

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Perspectives of air cargo activities in case of Debrecen International Airport
Published July 18, 2012
131-134

The improvement of Debrecen International Airport has been taking place for more than a decade. The goal is to revitalise the domestic and international aviation of regional centres and connect the Debrecen centred economy zone with other industrial and commercial regions. Considering the market segments of the air cargo industry, and sizing up... the requirements of the enterprises, we are seeking to answer for what possibilities an air cargo operation has, and scheduled cargo flights, what alternatives the location of a cargo base has. Getting to know the technical background of ground handling, communication, safety, and security, we can state that the airport is able to handle international cargo aircrafts as well. Regarding to the quantity of frights, we find that there is a possibility of two flights per week. There is minor need of handling dangerous or unique parcels. The surrounding area of working zone is an excellent territory for locating and building cargo facilities, and its technical background. In case of continuously increasing traffic it is suggestible to use the territory behind the apron as a first step of the development. As the activity extends the second step could be the improvement of the southern zone just beside the runway. This way primarily the
scheduled air cargo flights could appear, then as the traffic of commercial scheduled flights increases simultaneously could grow the amount of
small parcel transportation. 

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128
Studying the effects of traits in the genotype of three maize hybrids in Hungary
Published May 23, 2019
97-101

In this study, we selected three hybrids (Armagnac, Loupiac, and Sushi) for evaluation of the effect of traits in genotype in Debrecen. In 2017, the total rainfall from May to October was 314 mm in Debrecen, which was 236 mm in the winter period before sowing. The obtained results showed that there was a positive correlation between the weight ...of the cob maize and the rate of seed/cob, number of rows with number of seeds in column and outer diameter ear with weight of cob and number of rows in grain per ear and the rate of seed / cob; Also, there was a negative correlation between grain weight in ear with seed/cob rate, outer diameter ear and the rate of seed/cob and outer diameter ear with the number of leaves. There was a positive correlation between stem diameter, Seed/cob rate and the number of nodes by GGE biplot. In addition, there are traits of weight of all seeds and outer ear diameter that had the highest effect on average yield. Moreover, the number of seeds per row showed the least effect on the average yield of hybrids.

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The effect of water-stress on the mineral nutrition of fruit plantations
Published October 5, 2010
187-192

Besides agro-techniques the climatic conditions play an important role in agricultural production. Weather extremes are
significant hazards to many horticultural regions all over the word. It has a profound influence on the growth, development and yields of a
crop, incidence of pests and diseases, water needs and fertilizer requirements i...n terms of differences in nutrient mobilization due to water
stresses. Nowadays, the weather extremes cause more and more problems and significant hazards to many horticultural regions in Hungary.
The aim of this study is to explore the problems of nutrient uptake followed from climatic anomalies and response it. In this study
we focus on water supply problems (water-stress).
Reviewing the effects and nutrient disorders caused by climatic anomalies, the following statements can be taken:
· Nutrient demand of trees can be supplied only under even worse conditions.
· The most effective weapon against damage of climatic anomalies is preventative action.
· Proper choice of cultivars, species and cultivation should provide further possibilities to avoid and moderate the effects of
climatic anomalies.
· Fruit growing technologies especially nutrition should be corrected and adjusted to the climatic events as modifier factors.
· The role of foliar spraying, mulching and fertigation/irrigation is increasing continuously.
· Urgent task of the near future is to correct and adjust the tested technologies of fruit growing according to these climatic events as
modifier factors.
Optimal nutrient supply of trees decreases the sensitivity for unexpected climatic events. To solve these problems supplementary, foliar
fertilization is recommended, which adjusted to phonological phases of trees. Moreover, mulching is regarded as an excellent water saving
method.

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The effect of sowing date and plant density on the yield of maize (Zea mays L.) under different weather conditions
Published June 30, 2018
205-208

Maize has high productivity and produces huge vegetative and generative phytomass, but this crop is very sensitive to agroecological (mainly to climatic, partly to pedological conditions) and agrotechnical circumstances. In Hungary, maize is grown on 1.1–1.2 million hectares, the national average yields vary between 4–7 t ha-1 depending on ...the year and the intensity of production technology. The longterm experiment was set up in 2015–2016 on chernozem soil in the Hajdúság (eastern Hungary). The maize research was set up on chernozem soil at the Látókép MÉK (Faculty of Agricultural and Food Sciences and Environmental Management) research area of the University of Debrecen. We examined the following commonly used hybrids of Hungary: SY ARIOSO (FAO 300), P9074 (FAO 310), P9486 (FAO 360), SY Octavius (FAO 400), GK Kenéz (FAO 410), DKC 4943 (FAO 410). The experiment was set up in three different plant densities. These were 60, 76, 90 thousand plant ha-1. The experiment was set up with three different sowing dates, early, average and late sowing. The yield was measured using a special plot harvester (Sampo Rosenlew 2010), measuring the weight of the harvested plot and also taking a sample from it. As a next step, we calculated the yield (t ha-1) of each plot at 14% of moisture content to compare them to each other. We evaluated the obtained data using Microsoft Excel 2015.

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Evaluation of striptillage and conventional tillage in maize production
Published April 23, 2014
37-40

Tillage changes soil properties and the way how the environment affects those properties. Soil properties and environment determine the rate of water movement in liquid and gaseous form into and out of soil. Based on the experimental database of the Institute of Land Utilisation, Regional Development and Technology of the University of Debrecen..., Faculty of Agricultural and Food Sciences and Environmental Management and the KITE PLC, various cultivation systems were examined with maize (Zea mays L.) as indicator plant in Jász-Nagykun-Szolnok county. The sample area can be found in the outskirts of Kenderes on a meadow chernozem soil. On the examined plot, strip-tillage, subsoiling and moldboard ploughing were performed, each on 4.5 ha, respectively. The purpose of the present study is to compare these cultivation systems according to the soil- and maize kernel moisture content and to the yield based on the years of 2012 and 2013.

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Preliminary studies on in vitro sensitivity of Venturia inaequalis populations to some fungicide active ingredients
Published December 16, 2012
165-168

Aim of this study was to investigate the sensitivity of conidia of Spilocaea pomi to main fungicide active ingredients (cyprodinil, pirimethanil, fluquinconazole, tebuconazole, difenoconazole, dodine és trifloxistrobin) at dosages of 0.5, 1 and 2 times incubated in vitro at 24 and 48 hours collected from three integrated apple orchards. Result...s showed that degree of conidial germinations at the fungicide treatnemts decreased in the order of dosages of untreated, 0.5, 1 and 2 times. Considerable conidial germination was observed at higher dosages of some fungicides (e.g. groups of strobilurines and EBI) which confirmed the possibilities of sensitivity reduction of Spilocaea pomi to some funigicides in the sampled Hungarian apple orchards.

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Utilization and examination of red, elemental selenium nano spheres, produced by fermentation technology, in animal tests
Published November 13, 2012
245-247

In our experiments we tested the toxicity of Nano-Se and LactoMicroSel® compared with other organic and inorganic selenium forms, in case of a subakut animal test. We produced the Nano-Se and LactoMicroSel® by probiotic lactic acid bacteria in our laboratory. (Prokisch et al., 2010; Eszenyi et al., 2011). We mixed the inorganic selenium forms..., selenite and selenate, the organic form, Sel-Plex® and our products, Nano-Se and LactoMicroSel® into the standard food of laboratory mice and we fed them for two consecutive weeks. After the extermination we observed mortality, the change of body mass,and measured the blood antioxidant capacity with FRAP method.

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Comparison of alveohraph and extensigraph properties of winter wheat samples
Published November 2, 2009
27-30

The rheological properties of dough are determined by the amount and proportion of gliadin and glutenin proteins. Extensibility and resistance to the extension of doughs which can be measured using the extensigraph or alveograph allow good assesment of the baking behavior under realistic conditions. In our study, we compared the extensigraph an...d alveograph parameters of 87 flour samples. The results showed that the alveograph parameters were different from the extensigraph parameters. The classification of wheat varieties was different, and the order of varieties, as well. The alveograph properties were affected by other factors than the extensigraph properties, except the W value. The two rheological tests did not give similar results, therefor neither test are replaceable.

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Optimization of inductively coupled plasma mass spectrometer parameter’s to measuring arsenic and selenium
Published December 16, 2012
59-64

In the last decades an increased interest has been evolved about arsenic and selenium. The aim is to understand the environmental, agricultural and biological role of the these elements. In case of arsenic the mayor reasons are the relatively high concentration of arsenic in marine biota (mg kg-1) and the arsenic contaminated drinkin...g waterbases of some Asian countries besides Hungary. The toxicity of higher level selenium content is also known, nevertheless selenium is essential for some biological functions. Considering its esssentiality, in our country the insufficient selenium intake rate couse lack of selenium. Measuring the concentrations of these elements are cruital but not satisfactory information, but the speciation, that is the form of an element presented in a sample is also required. 
In both cases the most suitable method to determine concentration is the inductively coupled plasma mass spectrimetry. My objective was to optimase the changeable parameters of the ICP-MS for reaching the lowest (the best) detection limit. For this porpuse I have investigated the effect of parameter change on nett signal intensity and relative signal intensity. With the optimased parameter settings the limit of detection for arsenic and selenium were determined, which are 0,032 ng cm-3 for arsenic, and 0,097 ng cm-3 for
selenium.

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Morpometric study of Hungarian honey bee (Apis mellifera L.) colonies
Published February 10, 2013
59-63

...5); font-variant-ligatures: normal; font-variant-caps: normal; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial;">The honey bees are essential for the pollination of agricultural plants. The Pannonian honey bee, Apis mellifera pannonica, is native to Hungary, only these subspecies are being bread in our country. The parameters have been separated the pannon and italian honey bee subspecies, the colour of tergit, the cubital index and proboscis length. The morphometric analisys is of special importance because this, on the one hand, shows correlation with honey bee production and on the other hand, the pure morphometric charactersitics is the basis of any potential honey bee export. Mitochondrial DNA and microsatellites are the common methods to define genetic diversity and the separation of subspecies.

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Irén Dobos horticulturalist-teacher plant protection engineer expert awarded by Antal Gulyás medallion for crop protection" in 2015 (laudation)
Published June 2, 2015
8-10

The Public Utility for Development of Crop Protection Teaching (NOFKA) and The Hajdú-Bihar County Regional Association of Hungarian Chamber of Crop Protection Specialists and Plant Doctors established a joined Award Committee in 2011, which intend to serve as moral appreciation to prominent persons with excellent achievements by awarding the ...Antal Gulyás medallion for crop protection” which are available for outstanding teachers, researchers, and practical crop protection specialists. In 2015 dr. Irén Dobos horticulturist- teacher and plant doctor has been decorated with the „Antal Gulyás medallion for crop protection” for her “excellence in integrated plant protection practical research activity and effective knowledge transfer”.

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Analysis of maize and sunflower plants treated by molybdenum in rhizobox experiment
Published March 5, 2015
11-14

In this study, maize (Zea mays L. cv. Norma SC) and sunflower (Helianthus annuus L. cv Arena PR) seedlings treated by molybdenum (Mo) that were cultivated in special plant growth boxes, known as rhizoboxes. During our research we tried to examine whether increasing molybdenum (Mo) concentration effects on the dry mass and absorption of some ele...ments (molybdenum, iron, sulphur) of shoots and roots of experimental plants.

In this experiment calcareous chernozem soil was used and Mo was supplemented into the soil as ammonium molybdate [(NH4)6Mo7O24.4H2O] in four different concentrations as follow: 0 (control), 30, 90 and 270 mg kg-1.

In this study we found that molybdenum in small amount (30 mg kg-1) affected positively on growth of maize and sunflower seedlings, however, further increase of Mo content reduced the dry weights of shoots and roots. In case of maize the highest Mo treatment (270 mg kg-1) and in case of sunflower 90 mg kg-1 treatment caused a significant reduction in plant growth.

In addition, we observed that molybdenum levels in seedling were significantly elevated with increasing the concentration of molybdenum treatment in comparison with control but the applied molybdenum treatments did not affect iron and sulphur concentration in all cases significantly.

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Age of puberty and conception rates of Hungarian Merino, (Merino x Somali) F1 and (Merino x Barbados Blackbelly) F1 ewes
Published December 16, 2012
15-19

The aim of our examination was to detect the puberty of the Hungarian Merino and its hairsheep crossbred ewes, and also to determine their average conception rates after having ram exposed in May and June. Hormon diagnostation was used to evaluate the exact date of the very first ovulation. Ultrasound technique was used for checking the concept...ion rates of ewes. The pregnancy detection was applied after a month of the date of taking off the ram.
We evaluated the ages of ewes at puberty. Statistical deviation and standard error were calculated. The results proved the Hungarian Merino x Somali (N=10), ((x ± s) = 173 ± 43) and also the Hungarian Merino x Barbados Blackbelly crossbred genotypes (N=7), ((x ± s) = 186 ± 19) have their puberty much earlier as compared to the fullbred Hungarian Merino ewes (N=10), ((x ± s) = 231 ± 95). We experienced the highest conception rate in the group of the (Hungarian Merino x Somali) F1 ewes after exposing the ram in the „out-of season” period of time.

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Foreword
Published September 5, 2018
9-10

The higher education of economics and agriculture in Debrecen started 150 years ago. The historical roots of our institution reach back to the middle of the 19th century. In the 1850s, the wise leaders of the city of Debrecen realised that the development of agriculture is the sole opportunity which can make the region of the Tisza River and th...e Trans- Tisza region the most valuable pantry of the nation. Due to the generous support of the citizens of Debrecen, as well as the enthusiastic work of scientist and teachers, the National Higher Education Institute of Farming was established on 22nd October 1868 as the first agricultural higher education institution of the Great Hungarian Plain.

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Parameter optimization of an inductively coupled plasma mass spectrometer for measuring arsenic and selenium
Published November 20, 2011
81-85

In the last decades, an increased interest has evolved in arsenic and selenium. The aim is to understand the environmental, agricultural and biological roles of these elements. In the case of arsenic, the major reasons are the relatively high concentration of arsenic in marine biota (mg kg-1) and the arsenic contaminated drinking wat...er bases of some Asian countries, as well as Hungary. The toxicity of higher level selenium content is also known; nevertheless, selenium is essential for several biological functions. Considering its essentialness, in our country, the insufficient selenium intake rate causes a lack of selenium. Measuring the concentrations of these elements provides crucial, but unsatisfactory information, as the speciation, i.e. the form of an element presented in a sample is also required.
In both cases, the most suitable method to determine concentration is inductively coupled plasma mass spectrometry (ICP-MS). Our objective was to optimize the variable parameters of the ICP-MS to attain the lowest (the best) detection limit. For this purpose, we investigated the effect of parameter change on net signal intensity and relative signal intensity. With the optimized parameter settings, the limits of detection for arsenic and selenium were determined, which are 0,032 ng dm-3 for arsenic, and 0,097 ng dm-3 for selenium. 

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Mitigation of environment impact of mycotoxins produced by Fusarium species
Published December 16, 2012
159-164

The Fusarium fungi hazards the grain quality of cereals, therefore significantly affects their utilization as animal feed or consumable product. The Fusarium can decrease the quality of wheat in different ways: decreases the germination capability, causes visible discoloration, mould may appear, reduces the dry material and nutrient content of ...the grain, causes mycotoxin infection – as a result given by its by-product. Micotoxins produced by Fusarium genus, as the trichotecenes (T-2, HT-2, deoxynivalenol, nivalenol, diacetoxyscirpenol, Fusarenone-X) and the zearalenone (F-2) are the most common in Hungary. Occurrence of fumonisins first discovered in 1988 are must be identified carefully. About 20–30% of the overall worldwide production of cereals is infected with Fusarium and its toxins, which situation is similar in Hungary. This infection causes serious yield-losses in cereal production. In the case of cereal products, which non-utilizable as forage seems, an optimal solution is utilizing as biogas raw material, but it is also important to examine the effect of the infected cereal on the anaerobe digestion process.

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The scientific background of competitive maize production
Published September 5, 2018
33-46

The effect and interaction of crop production factors on maize yield has been examined for nearly 40 years at the Látókép Experiment Site of the University of Debrecen in a long-term field experiment that is unique and acknowledged in Europe. The research aim is to evaluate the effect of fertilisation, tillage, genotype, sowing, plant densit...y, crop protection and irrigation. The analysis of the database of the examined period makes it possible to evaluate maize yield, as well as the effect of crop production factors and crop year, as well as the interaction between these factors.

Based on the different tillage methods, it can be concluded that autumn ploughing provides the highest yield, but its effect significantly differed in irrigated and non-irrigated treatments. The periodical application of strip tillage is justified in areas with favourable soil conditions and free from compated layers (e.g. strip – strip – ploughing – loosening). Under conditions prone to drought, but especially in several consecutive years, a plant density of 70–80 thousand crops per hectare should be used in the case of favourable precipitation supply, but 60 thousand crops per hectare should not be exceeded in dry crop years. The yield increasing effect of fertilisation is significant both under non-irrigated and irrigated conditions, but it is much more moderate in the non-irrigated treatment.

Selecting the optimum sowing date is of key importance from the aspect of maize yield, especially in dry crop years. Irrigation is not enough in itself without intensive nutrient management, since it may lead to yield decrease.

The results of research, development and innovation, which are based on the performed long-term field experiment, contribute to the production technological methods which provide an opportunity to use sowing seeds, fertilisers and pesticides in a regionally tailored and differentiated way, adapted to the specific needs of the given plot, as well as to plan each operation and to implement precision maize production.

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Young-making of sour cherry cultivars and examination of their regenerative characteristics
Published May 23, 2006
102-105

Although a high portion of domestic orchards is cultivated extensively, quality Hungarian sour cherry is a highly demanded product throughout Europe. Trees are slightly pruned, or not pruned at all. Thanks to the unique character of the species, older wood parts are pilling up. Thus, the interior of the canopy is inactive, fruits and shoots con...fine the exterior layer of the canopy.
We established pruning treatments in the sour cherry plantation in the Horticultural Exhibition Garden of the University of Debrecen, involving two age groups in two spacings. The trees are standing on seedling rootstock. The involved varieties were: Érdi bőtermő, Debreceni bőtermő and Kántorjánosi. We examined how to encourage shoot formation and the regeneration of older, pilling wood of the interior canopy parts.
Applying strong rejuvenating cuts, the most shoots were generated on the 3rd and 4th year laterals. Wood parts older than the 3rd year shall be removed by leaving short (2-3cm) stubs. This way, older, passive woodparts can also be made to burst out. From the examined varieties the Kántorjánosi showed the most favourable regenerative ability. Due to summer pruning, more shoots regenerated on the axis of the trees, than on those rejuvenated in the dormant season.

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Studying of quality parameters of Hungarian and Greek honey samples
Published November 15, 2007
147-153

Honey has been a valuable food for mankind since ancient times. It was the only sweetener until the start of industrial sugar mass production. Honey plays an important role in our nutrition and its positive effects on health are well-known. The quality of Hungarian honey is perfect, so it is very important to safeguard and monitor its quality c...ontinuously and to build up a good traceability and quality assurance system. For such a system, it is necessary to study the nutritional properties and the origin of different honey samples. In our study, we study Hungarian and Greek honey.

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Leaf protein analyses in order to utilise the leaf shoot of artichoke
Published February 3, 2016
43-47

The constat growth of the Earth’s population brings with itself a higher demand for food and protein not only in human nutrition but also for the feeding of livestock. Currently, the feed industry is mainly built onseed-based protein, wherethebaseplant is soybean, which is large lycovered by imports in Hungary, similar toother European countr...ies. However, the long-term economically sustainable lifestock breeding demandschanges which has also worked out strategies. An alternative protein sources could be green leafy plants.

In current work the Jerusalem artichokes as an alternative source of protein was studied, compared to alfalfa as a valuable protein plant. Our results indicate that fiber fraction ofJerusalem artichoke shootswas 34 to 37% after pressing in the autumn period while alfalfa slightly lower values were obtained (30%). On the other hand extracted green leaf protein concentration was higher in alfalfa than in Jerusalem artichoke. Along with this higher protein content could be measured from the leaf protein concentration of alfalfa and almost each amino acids were more, as well comparing to Jerusalem artichoke.

Overall, the alfalfa proved to be advantageous as expected both in leaf protein extraction efficiency, both regarding the content of the protein in the Jerusalem artichoke. However, considering aminoacid composition and green biomass production, Jerusalem artichokecould be a promisingplant species asplant protein sourceinthefuture.

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Effect of arsenic treatments on physiological parameters of sunflower and maize plants
Published March 5, 2015
81-84

The environment is contaminated with heavy metals and other toxic compounds. One of the most important toxic element is the arsenic (As).

The objective of our study was to investigate the effect of As on fresh and dry weight of sunflower and maize in the early growth phases.

Seedlings were grown in climate room on nutrient solut...ion which were treated with 3, 10 and 30 mg kg-1 arsenic. The plants were treated separately with As(III) and As(V). After 14 day, changes in fresh and dry weight of maize shoots and roots were recorded. In the case of sunflower these parameters were measured after 21 day.

The applied As(III) and As(V) decreased the fresh and dry mass of the shoots and roots of seedlings, especially at concentration 30 mg kg-1. We can draw the conclusion that the treatments of the maize and sunflower roots with arsenic had negative effects on the biomass accumulation. We found that the sunflower plants are more sensitive to arsenic toxicity than maize plants, and all data demonstrate that the As(III) is more toxic to these plants than the As(V).

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Evaluating of soil sulphur forms changes in long-term field experiments of Látókép
Published December 15, 2019
71-76

The aim of this work was to evaluate the changes of different sulphur forms (soluble, adsorbed) in chernozem soil in a long-term field experiment supplied with increasing doses of NPK fertilizers for a long time. In addition, other objective of this study included the examination of the applicability of recommended extractants of the different ...sulphate fraction in Hungarian soils. A long-term field experiment was established at the Research Station of Látókép of the University of Debrecen in 1984. In addition to control, two levels of NPK fertilizer doses have been used with irrigated and non-irrigated variants. Winter wheat and corn were cropped in a crop rotation on plots. Soil samples were collected in three different development stages of winter wheat, at the stage of stem elongation (April), flowering (May) and ripening (June of 2018) from the topsoil (0–20 cm) of experiment plots. Water-soluble inorganic sulphate was extracted with 0.01M CaCl2 solutions. The soluble plus adsorbed sulphate was extracted with 0.016M KH2PO4 solution. Sulphate was measured by turbidimetric method. 0.01M CaCl2-SO42— ranged between 0.293–1.896 mg kg-1 and the 0.016 M KH2PO4-SO42- varied between 5.087–10.261 mg kg-1. The values of KH2PO4 SO42- was higher than that of CaCl2-SO42-, because KH2PO4 extracted the adsorbed and soluble fractions of sulphate, while CaCl2 extracted the soluble sulphate fraction. The amount of absorbed sulphate was calculated by the differences of KH2PO4- SO4 and CaCl2-SO4. The KH2PO4 characterizes mainly the adsorbed sulphate fraction much more than the water-soluble fraction. KCl is the most widely used extractant for the determination of plant available sulphate content of soil in Hungary; therefore, KCl-SO42- fraction also was determined. The KCl-SO42- ranged between 0.328–2.152 mg kg-1. The CaCl2-SO42- and KCl-SO42- fractions were compared and based on Pearson's linear correlation, moderate correlation was established (r=0.511) between them. In all three extractant (0.01M CaCl2, 1M KCl, 0.016 M KH2PO4) higher sulphate fractions were measured in the fertilized plots where superphosphate had been supplied for ages until 2010. The arylsulphatase activity of soil also was determined and ranged between 9.284 and 26.860 µg p-nitrophenol g-1 h-1. The lowest value was observed in the treatment with highest NPK2 dose, both in irrigated and non-irrigated areas.

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Occurrence and damage of the alfalfa root longhorn beetle (Plagionotus floralis Pallas, 1773) in old alfalfa stands in Hungary
Published November 10, 2010
82-88

The alfalfa root longhorn beetle is a pest of small importance in old alfalfa stands. It is rarely cited in the international, national literature, or even mentioned in specialized manuels. Plagionotus floralis is common in Hungary but its population density is low. It is a polyphagous species but it has importance - regarding the references ... only in alfalfa. This paper analyses data gained in old alfalfa fields at two different regions of the country (Máriabesnyő, Debrecen). Damage of P. floralis was 56% in the roots of an 8-year-old stand in Máriabesnyő. Lenghts of tunnels were between 3 and 22 cm. In spite of the tunnels the alfalfa plants were viable. There was no root longhorn beetle in the 15-year-old Debrecen stand, however it has been found former in the region. The reason for the important density of P. floralis in Máriabesnyő could be the considerable diversity and covering of flowering weeds around the field. Adults of P. floralis feed on flower pollen and nectar. As to the Debrecen area,weeds were cut regularly, so the edge vegetation was poor. Present conditions (no pest control on alfalfa fields, overuse of old and failing alfalfa stands, many untrained growers) favour the reproduction and possible damage of alfalfa root longhorn beetle. 

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